Restriction map for
pFN21AB3831
(Linear) (Six-base) MAPSORT of: KIBB3831.seq Check: 1640 from: 1 to: 2103
KIBB3831.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 393 698 2103
Size: 393 305 1405
Fragments arranged by size:
1405 393 305
AatII G_ACGT'C
Cuts at: 0 218 2103
Size: 218 1885
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 360 2103
Size: 112 248 1743
Fragments arranged by size:
1743 248 112
AfeI AGC'GCT
Cuts at: 0 940 2103
Size: 940 1163
AflIII A'CryG_T
Cuts at: 0 779 2103
Size: 779 1324
AhdI GACnn_n'nnGTC
Cuts at: 0 219 2103
Size: 219 1884
AleI CACnn'nnGTG
Cuts at: 0 910 1264 2103
Size: 910 354 839
Fragments arranged by size:
910 839 354
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1549 1945 2103
Size: 1549 396 158
Fragments arranged by size:
1549 396 158
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2011 2043 2103
Size: 2011 32 60
Fragments arranged by size:
2011 60 32
AlwNI CAG_nnn'CTG
Cuts at: 0 156 630 1128 1222 1896 2103
Size: 156 474 498 94 674 207
Fragments arranged by size:
674 498 474 207 156 94
ApaI G_GGCC'C
Cuts at: 0 725 1359 1783 1917 1999 2103
Size: 725 634 424 134 82 104
Fragments arranged by size:
725 634 424 134 104 82
ApaLI G'TGCA_C
Cuts at: 0 2038 2103
Size: 2038 65
AvaI C'yCGr_G
Cuts at: 0 71 948 1987 1999 2103
Size: 71 877 1039 12 104
Fragments arranged by size:
1039 877 104 71 12
BamHI G'GATC_C
Cuts at: 0 704 2103
Size: 704 1399
BanI G'GyrC_C
Cuts at: 0 42 249 346 713 763 867 1290 1426
Size: 42 207 97 367 50 104 423 136
Cuts at: 1426 1806 2103
Size: 380 297
Fragments arranged by size:
423 380 367 297 207 136 104 97
50 42
BanII G_rGCy'C
Cuts at: 0 422 725 839 1004 1359 1402 1604 1732
Size: 422 303 114 165 355 43 202 128
Cuts at: 1732 1783 1917 1999 2067 2103
Size: 51 134 82 68 36
Fragments arranged by size:
422 355 303 202 165 134 128 114
82 68 51 43 36
BbeI G_GCGC'C
Cuts at: 0 767 2103
Size: 767 1336
BbsI GAAGACnn'nnnn_
Cuts at: 0 1219 2103
Size: 1219 884
BbvCI CC'TCA_GC
Cuts at: 0 2012 2103
Size: 2012 91
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 625 659 2103
Size: 625 34 1444
Fragments arranged by size:
1444 625 34
BglI GCCn_nnn'nGGC
Cuts at: 0 652 772 1051 1195 1505 1537 2103
Size: 652 120 279 144 310 32 566
Fragments arranged by size:
652 566 310 279 144 120 32
BlpI GC'TnA_GC
Cuts at: 0 748 1495 2103
Size: 748 747 608
Fragments arranged by size:
748 747 608
Bme1580I G_kGCm'C
Cuts at: 0 349 725 1359 1783 1811 1917 1999 2042
Size: 349 376 634 424 28 106 82 43
Cuts at: 2042 2103
Size: 61
Fragments arranged by size:
634 424 376 349 106 82 61 43
28
BmgBI CAC'GTC
Cuts at: 0 789 2103
Size: 789 1314
BmtI G_CTAG'C
Cuts at: 0 826 1939 2103
Size: 826 1113 164
Fragments arranged by size:
1113 826 164
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1746 2002 2103
Size: 1746 256 101
Fragments arranged by size:
1746 256 101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 2101 2103
Size: 406 1695 2
Fragments arranged by size:
1695 406 2
Bpu10I CC'TnA_GC
Cuts at: 0 14 1598 2012 2103
Size: 14 1584 414 91
Fragments arranged by size:
1584 414 91 14
BsaBI GATnn'nnATC
Cuts at: 0 2096 2103
Size: 2096 7
BsaHI Gr'CG_yC
Cuts at: 0 215 587 764 1478 2103
Size: 215 372 177 714 625
Fragments arranged by size:
714 625 372 215 177
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2011 2041 2103
Size: 2011 30 62
Fragments arranged by size:
2011 62 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1991 2029 2032 2103
Size: 1991 38 3 71
Fragments arranged by size:
1991 71 38 3
BseYI C'CCAG_C
Cuts at: 0 63 597 693 2103
Size: 63 534 96 1410
Fragments arranged by size:
1410 534 96 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 141 169 2071 2103
Size: 129 12 28 1902 32
Fragments arranged by size:
1902 129 32 28 12
BsiEI CG_ry'CG
Cuts at: 0 1509 1820 2103
Size: 1509 311 283
Fragments arranged by size:
1509 311 283
BsiHKAI G_wGCw'C
Cuts at: 0 422 839 1885 2042 2103
Size: 422 417 1046 157 61
Fragments arranged by size:
1046 422 417 157 61
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1729 2103
Size: 1729 374
Bsp1286I G_dGCh'C
Cuts at: 0 349 422 725 839 1004 1359 1402 1604
Size: 349 73 303 114 165 355 43 202
Cuts at: 1604 1732 1783 1811 1885 1917 1999 2042 2067
Size: 128 51 28 74 32 82 43 25
Cuts at: 2067 2103
Size: 36
Fragments arranged by size:
355 349 303 202 165 128 114 82
74 73 51 43 43 36 32 28
25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 393 698 1904 2103
Size: 164 229 305 1206 199
Fragments arranged by size:
1206 305 229 199 164
BsrDI GCAATG_nn'
Cuts at: 0 271 2103
Size: 271 1832
BsrFI r'CCGG_y
Cuts at: 0 39 45 430 1504 1819 1873 2103
Size: 39 6 385 1074 315 54 230
Fragments arranged by size:
1074 385 315 230 54 39 6
BssSI C'ACGA_G
Cuts at: 0 117 910 1048 2060 2103
Size: 117 793 138 1012 43
Fragments arranged by size:
1012 793 138 117 43
BstAPI GCAn_nnn'nTGC
Cuts at: 0 156 806 1896 2103
Size: 156 650 1090 207
Fragments arranged by size:
1090 650 207 156
BstEII G'GTnAC_C
Cuts at: 0 792 2103
Size: 792 1311
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1074 1206 2103
Size: 1074 132 897
Fragments arranged by size:
1074 897 132
BstYI r'GATC_y
Cuts at: 0 704 2103
Size: 704 1399
Bsu36I CC'TnA_GG
Cuts at: 0 862 989 2103
Size: 862 127 1114
Fragments arranged by size:
1114 862 127
BtgI C'CryG_G
Cuts at: 0 193 365 647 709 878 971 1034 1262
Size: 193 172 282 62 169 93 63 228
Cuts at: 1262 1381 1430 2103
Size: 119 49 673
Fragments arranged by size:
673 282 228 193 172 169 119 93
63 62 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 246 2103
Size: 246 1857
BtsI GCAGTG_nn'
Cuts at: 0 129 628 1170 2103
Size: 129 499 542 933
Fragments arranged by size:
933 542 499 129
DraIII CAC_nnn'GTG
Cuts at: 0 806 1833 2103
Size: 806 1027 270
Fragments arranged by size:
1027 806 270
DrdI GACnn_nn'nnGTC
Cuts at: 0 1239 1414 2103
Size: 1239 175 689
Fragments arranged by size:
1239 689 175
EaeI y'GGCC_r
Cuts at: 0 278 428 902 1196 1433 1502 1506 1817
Size: 278 150 474 294 237 69 4 311
Cuts at: 1817 2103
Size: 286
Fragments arranged by size:
474 311 294 286 278 237 150 69
4
EagI C'GGCC_G
Cuts at: 0 1506 1817 2103
Size: 1506 311 286
Fragments arranged by size:
1506 311 286
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 600 1093 1407 2103
Size: 600 493 314 696
Fragments arranged by size:
696 600 493 314
EcoICRI GAG'CTC
Cuts at: 0 420 837 2103
Size: 420 417 1266
Fragments arranged by size:
1266 420 417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 360 406 2101 2103
Size: 112 248 46 1695 2
Fragments arranged by size:
1695 248 112 46 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1363 1990 2103
Size: 1363 627 113
Fragments arranged by size:
1363 627 113
EcoO109I rG'GnC_Cy
Cuts at: 0 153 576 653 721 993 1355 1356 1374
Size: 153 423 77 68 272 362 1 18
Cuts at: 1374 1587 1756 1773 1893 1913 1914 1995 1996
Size: 213 169 17 120 20 1 81 1
Cuts at: 1996 2103
Size: 107
Fragments arranged by size:
423 362 272 213 169 153 120 107
81 77 68 20 18 17 1 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1674 2103
Size: 1674 429
FseI GG_CCGG'CC
Cuts at: 0 1508 2103
Size: 1508 595
FspI TGC'GCA
Cuts at: 0 1363 2103
Size: 1363 740
HaeII r_GCGC'y
Cuts at: 0 63 145 767 942 1190 1259 1288 2103
Size: 63 82 622 175 248 69 29 815
Fragments arranged by size:
815 622 248 175 82 69 63 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 2103
Size: 205 32 1866
Fragments arranged by size:
1866 205 32
KasI G'GCGC_C
Cuts at: 0 763 2103
Size: 763 1340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 2103
Size: 75 2028
MscI TGG'CCA
Cuts at: 0 904 1198 2103
Size: 904 294 905
Fragments arranged by size:
905 904 294
MslI CAynn'nnrTG
Cuts at: 0 247 556 898 910 1264 1441 2103
Size: 247 309 342 12 354 177 662
Fragments arranged by size:
662 354 342 309 247 177 12
MspA1I CmG'CkG
Cuts at: 0 113 195 426 711 873 1239 1296 1383
Size: 113 82 231 285 162 366 57 87
Cuts at: 1383 1432 1635 1816 1854 2025 2103
Size: 49 203 181 38 171 78
Fragments arranged by size:
366 285 231 203 181 171 162 113
87 82 78 57 49 38
NaeI GCC'GGC
Cuts at: 0 432 1506 1821 2103
Size: 432 1074 315 282
Fragments arranged by size:
1074 432 315 282
NarI GG'CG_CC
Cuts at: 0 764 2103
Size: 764 1339
NcoI C'CATG_G
Cuts at: 0 647 2103
Size: 647 1456
NgoMIV G'CCGG_C
Cuts at: 0 430 1504 1819 2103
Size: 430 1074 315 284
Fragments arranged by size:
1074 430 315 284
NheI G'CTAG_C
Cuts at: 0 822 1935 2103
Size: 822 1113 168
Fragments arranged by size:
1113 822 168
NspI r_CATG'y
Cuts at: 0 361 2103
Size: 361 1742
PasI CC'CwG_GG
Cuts at: 0 1764 1917 2103
Size: 1764 153 186
Fragments arranged by size:
1764 186 153
PflMI CCAn_nnn'nTGG
Cuts at: 0 104 173 1833 2103
Size: 104 69 1660 270
Fragments arranged by size:
1660 270 104 69
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2011 2043 2103
Size: 2011 32 60
Fragments arranged by size:
2011 60 32
PpuMI rG'GwC_Cy
Cuts at: 0 153 576 993 1374 1773 1893 2103
Size: 153 423 417 381 399 120 210
Fragments arranged by size:
423 417 399 381 210 153 120
PshAI GACnn'nnGTC
Cuts at: 0 1741 2103
Size: 1741 362
PspOMI G'GGCC_C
Cuts at: 0 721 1355 1779 1913 1995 2103
Size: 721 634 424 134 82 108
Fragments arranged by size:
721 634 424 134 108 82
PspXI vC'TCGA_Gb
Cuts at: 0 948 2103
Size: 948 1155
PstI C_TGCA'G
Cuts at: 0 26 290 461 1667 1892 1948 2103
Size: 26 264 171 1206 225 56 155
Fragments arranged by size:
1206 264 225 171 155 56 26
PvuII CAG'CTG
Cuts at: 0 113 426 1239 2025 2103
Size: 113 313 813 786 78
Fragments arranged by size:
813 786 313 113 78
RsrII CG'GwC_CG
Cuts at: 0 48 2103
Size: 48 2055
SacI G_AGCT'C
Cuts at: 0 422 839 2103
Size: 422 417 1264
Fragments arranged by size:
1264 422 417
SacII CC_GC'GG
Cuts at: 0 196 712 1384 1433 2103
Size: 196 516 672 49 670
Fragments arranged by size:
672 670 516 196 49
SexAI A'CCwGG_T
Cuts at: 0 572 2103
Size: 572 1531
SfcI C'TryA_G
Cuts at: 0 22 286 297 457 1309 1663 1674 1888
Size: 22 264 11 160 852 354 11 214
Cuts at: 1888 1944 2103
Size: 56 159
Fragments arranged by size:
852 354 264 214 160 159 56 22
11 11
SfoI GGC'GCC
Cuts at: 0 765 2103
Size: 765 1338
SmaI CCC'GGG
Cuts at: 0 73 2001 2103
Size: 73 1928 102
Fragments arranged by size:
1928 102 73
SmlI C'TyrA_G
Cuts at: 0 948 2103
Size: 948 1155
StyI C'CwwG_G
Cuts at: 0 647 2103
Size: 647 1456
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1528 2103
Size: 1528 575
TatI w'GTAC_w
Cuts at: 0 1637 2103
Size: 1637 466
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1283 1315 1684 1716 2103
Size: 1283 32 369 32 387
Fragments arranged by size:
1283 387 369 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 790 2103
Size: 790 1313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 885 940 1191 1443 1800 2103
Size: 885 55 251 252 357 303
Fragments arranged by size:
885 357 303 252 251 55
XhoI C'TCGA_G
Cuts at: 0 948 2103
Size: 948 1155
XmaI C'CCGG_G
Cuts at: 0 71 1999 2103
Size: 71 1928 104
Fragments arranged by size:
1928 104 71
ZraI GAC'GTC
Cuts at: 0 216 2103
Size: 216 1887
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI
BmtI BplI BpmI Bpu10I BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI
BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII DrdI EaeI EagI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PflMI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuII RsrII SacI SacII SexAI
SfcI SfoI SmaI SmlI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI ApoI AscI AseI
AsiSI AvrII BaeI BciVI BclI BfrBI BglII BmrI
BpuEI BsaI BsaAI BsaWI BsiWI BsmI BspEI BspHI
BsrBI BsrGI BssHII BstBI BstZ17I ClaI CspCI DraI
EarI EcoRI EcoRV FspAI HincII HindIII HpaI KpnI
MfeI MluI NdeI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PsiI PsrI PvuI SalI SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XmnI