Restriction map for
pFN21AB3827
##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
ApaI AscI AseI AsnI Asp718I
AspI AviII BbvCI BciVI BfrI
BsiWI BspDI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
EcoO65I FseI FspI HpaI KpnI
MfeI MluI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI SbfI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 1336 GTCTAC GT^[AC][GT]AC
AccIII 458 TCCGGA T^CCGGA
AhdI 2441 GACACAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1381 CCTGAGG CC^T[ACGT]AGG
Aor51HI 255 AGCGCT AGC^GCT
Asp700I 1467 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2441 GACACAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 157 CCTAGG C^CTAGG
BamHI 3120 GGATCC G^GATCC
BcgI 2726 CGACGGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 158 CTAG C^TAG
BlnI 157 CCTAGG C^CTAGG
BlpI 39 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 39 GCTGAGC GC^T[ACGT]AGC
BsaBI 1255 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 458 TCCGGA T^CCGGA
BsmBI 324 GAGACG CGTCTC(1/5)
Bsp1407I 1057 TGTACA T^GTACA
BspEI 458 TCCGGA T^CCGGA
BspLU11I 2652 ACATGT A^CATGT
BsrGI 1057 TGTACA T^GTACA
BssHII 44 GCGCGC G^CGCGC
Bsu36I 1381 CCTGAGG CC^T[ACGT]AGG
CelII 39 GCTGAGC GC^T[ACGT]AGC
DraI 3364 TTTAAA TTT^AAA
DraIII 1328 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2632 GACAGCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 315 CGGCCG C^GGCCG
Eam1105I 2441 GACACAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 315 CGGCCG C^GGCCG
Eco47III 255 AGCGCT AGC^GCT
Eco52I 315 CGGCCG C^GGCCG
Eco81I 1381 CCTGAGG CC^T[ACGT]AGG
EcoRI 2923 GAATTC G^AATTC
EcoRV 3165 GATATC GAT^ATC
KspI 2389 CCGCGG CCGC^GG
MaeI 158 CTAG C^TAG
MamI 1255 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 458 TCCGGA T^CCGGA
PflMI 522 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1510 CTGCAG CTGCA^G
SacI 612 GAGCTC GAGCT^C
SacII 2389 CCGCGG CCGC^GG
SapI 1750 GAAGAGC GCTCTTC(1/4)
ScaI 2296 AGTACT AGT^ACT
SexAI 3411 ACCTGGT A^CC[AT]GGT
SmlI 1000 CTTGAG C^T[CT][GA]AG
SspBI 1057 TGTACA T^GTACA
Van91I 522 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2499 CCAAGTGGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1467 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 901 GAATTT [GA]^AATT[CT]
2923 GAATTC
Alw44I 1546 GTGCAC G^TGCAC
2677 GTGCAC
ApaLI 1546 GTGCAC G^TGCAC
2677 GTGCAC
ApoI 901 GAATTT [GA]^AATT[CT]
2923 GAATTC
BalI 543 TGGCCA TGG^CCA
2217 TGGCCA
BbeI 13 GGCGCC GGCGC^C
1515 GGCGCC
BbrPI 1328 CACGTG CAC^GTG
2674 CACGTG
BbsI 850 GAAGAC GAAGAC(2/6)
1467 GAAGAC
BclI 767 TGATCA T^GATCA
2801 TGATCA
BglI 1379 GCCCTGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2747 GCCAAGTGGGC
BglII 1739 AGATCT A^GATCT
1852 AGATCT
BpuAI 850 GAAGAC GAAGAC(2/6)
1467 GAAGAC
BsaWI 458 TCCGGA [AT]^CCGG[AT]
2927 TCCGGT
BsiEI 315 CGGCCG CG[GA][CT]^CG
3003 CGGTCG
BspHI 1394 TCATGA T^CATGA
1934 TCATGA
BspMI 2607 GCAGGT ACCTGC(4/8)
2865 GCAGGT
BsrDI 1620 CATTGC GCAATG(2/0)
2213 GCAATG
BstAPI 1548 GCACTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3490 GCACAGTATGC
EcoT22I 1190 ATGCAT ATGCA^T
1237 ATGCAT
FbaI 767 TGATCA T^GATCA
2801 TGATCA
HgaI 89 GCGTC GACGC(5/10)
222 GCGTC
HincII 627 GTCAAC GT[CT]^[GA]AC
3144 GTCAAC
HindII 627 GTCAAC GT[CT]^[GA]AC
3144 GTCAAC
HindIII 825 AAGCTT A^AGCTT
1207 AAGCTT
KasI 13 GGCGCC G^GCGCC
1515 GGCGCC
MluNI 543 TGGCCA TGG^CCA
2217 TGGCCA
MscI 543 TGGCCA TGG^CCA
2217 TGGCCA
MseI 3256 TTAA T^TAA
3365 TTAA
NarI 13 GGCGCC GG^CGCC
1515 GGCGCC
NsiI 1190 ATGCAT ATGCA^T
1237 ATGCAT
PmaCI 1328 CACGTG CAC^GTG
2674 CACGTG
PmlI 1328 CACGTG CAC^GTG
2674 CACGTG
PpuMI 434 GGGACCC [GA]G^G[AT]CC[CT]
1538 GGGACCC
PshAI 2614 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2998 GACATCGGTC
RcaI 1394 TCATGA T^CATGA
1934 TCATGA
SfcI 1510 CTGCAG C^T[GA][CT]AG
2031 CTACAG
SfoI 13 GGCGCC GGC^GCC
1515 GGCGCC
SgrAI 15 CGCCGGCG C[GA]^CCGG[CT]G
54 CGCCGGCG
Tru9I 3256 TTAA T^TAA
3365 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SmaI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI