(Circular) (Six-base) MAPSORT of: pFN21AB3827.seq Check: 7259 from: 1 to: 8209 pFN21AB3827 8209 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4603 4603 Size: 8209 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6019 278 Size: 53 83 186 936 4483 2468 Fragments arranged by size: 4483 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3342 5552 1937 Size: 1405 2210 4594 Fragments arranged by size: 4594 2210 1405 Acc65I G'GTAC_C Cuts at: 5530 5530 Size: 8209 AclI AA'CG_TT Cuts at: 1563 6336 6709 1563 Size: 4773 373 3063 Fragments arranged by size: 4773 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3611 3702 3943 4816 4827 5243 6272 7656 Size: 1979 91 241 873 11 416 1029 1384 Cuts at: 7656 7924 1632 Size: 268 1917 Fragments arranged by size: 1979 1917 1384 1029 873 416 268 241 91 11 AfeI AGC'GCT Cuts at: 2262 2262 Size: 8209 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8012 AflIII A'CryG_T Cuts at: 1203 3177 3306 3332 4573 4657 7108 8085 1203 Size: 1974 129 26 1241 84 2451 977 1327 Fragments arranged by size: 2451 1974 1327 1241 977 129 84 26 AgeI A'CCGG_T Cuts at: 7040 7040 Size: 8209 AhdI GACnn_n'nnGTC Cuts at: 4451 6938 4451 Size: 2487 5722 AleI CACnn'nnGTG Cuts at: 1166 4772 1166 Size: 3606 4603 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8209 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5094 5126 5094 Size: 32 8177 AlwNI CAG_nnn'CTG Cuts at: 3504 3695 4231 5952 7524 3504 Size: 191 536 1721 1572 4189 Fragments arranged by size: 4189 1721 1572 536 191 ApaLI G'TGCA_C Cuts at: 3551 4682 6266 7422 3551 Size: 1131 1584 1156 4338 Fragments arranged by size: 4338 1584 1156 1131 ApoI r'AATT_y Cuts at: 1493 2906 4928 5518 5744 5896 8005 1493 Size: 1413 2022 590 226 152 2109 1697 Fragments arranged by size: 2109 2022 1697 1413 590 226 152 AseI AT'TA_AT Cuts at: 160 1032 6763 160 Size: 872 5731 1606 Fragments arranged by size: 5731 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8209 AvaI C'yCGr_G Cuts at: 1870 1943 2089 2249 2280 2388 2430 5534 1870 Size: 73 146 160 31 108 42 3104 4545 Fragments arranged by size: 4545 3104 160 146 108 73 42 31 AvrII C'CTAG_G Cuts at: 2162 2162 Size: 8209 BamHI G'GATC_C Cuts at: 5125 5539 5906 5125 Size: 414 367 7428 Fragments arranged by size: 7428 414 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 3520 4760 5530 6985 Size: 325 217 627 231 1502 1240 770 1455 Cuts at: 6985 618 Size: 1842 Fragments arranged by size: 1842 1502 1455 1240 770 627 325 231 217 BanII G_rGCy'C Cuts at: 1391 2073 2200 2281 2387 2621 5528 1391 Size: 682 127 81 106 234 2907 4072 Fragments arranged by size: 4072 2907 682 234 127 106 81 BbeI G_GCGC'C Cuts at: 2022 3524 2022 Size: 1502 6707 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2862 3479 928 Size: 971 963 617 5658 Fragments arranged by size: 5658 971 963 617 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4720 4754 6400 6434 1903 Size: 34 2783 34 1646 34 3678 Fragments arranged by size: 3678 2783 1646 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6101 7311 6101 Size: 1210 6999 BclI T'GATC_A Cuts at: 1548 2772 4806 1548 Size: 1224 2034 4951 Fragments arranged by size: 4951 2034 1224 BfrBI ATG'CAT Cuts at: 3197 3244 3197 Size: 47 8162 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3390 4758 6820 Size: 107 122 71 631 605 1718 1368 2062 Cuts at: 6820 136 Size: 1525 Fragments arranged by size: 2062 1718 1525 1368 631 605 122 107 71 BglII A'GATC_T Cuts at: 3744 3857 8204 3744 Size: 113 4347 3749 Fragments arranged by size: 4347 3749 113 BlpI GC'TnA_GC Cuts at: 2045 5626 2045 Size: 3581 4628 Bme1580I G_kGCm'C Cuts at: 1790 2288 3555 4382 4686 4763 5497 6270 7426 Size: 498 1267 827 304 77 734 773 1156 Cuts at: 7426 1790 Size: 2573 Fragments arranged by size: 2573 1267 1156 827 773 734 498 304 77 BmgBI CAC'GTC Cuts at: 1318 4576 1318 Size: 3258 4951 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2298 4314 5940 Size: 416 53 83 399 24 881 2016 1626 Cuts at: 5940 6898 442 Size: 958 1753 Fragments arranged by size: 2016 1753 1626 958 881 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8209 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2291 3757 3942 5154 5183 6869 1239 Size: 1052 1466 185 1212 29 1686 2579 Fragments arranged by size: 2579 1686 1466 1212 1052 185 29 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3026 6334 7199 7497 3026 Size: 3308 865 298 3738 Fragments arranged by size: 3738 3308 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3601 4879 4941 6872 882 Size: 617 2102 1278 62 1931 2219 Fragments arranged by size: 2219 2102 1931 1278 617 62 BsaAI yAC'GTr Cuts at: 493 1204 3178 3309 3335 4681 6000 493 Size: 711 1974 131 26 1346 1319 2702 Fragments arranged by size: 2702 1974 1346 1319 711 131 26 BsaBI GATnn'nnATC Cuts at: 3264 8203 3264 Size: 4939 3270 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2019 2094 3521 6016 Size: 53 83 186 936 486 75 1427 2495 Cuts at: 6016 6398 7033 275 Size: 382 635 1451 Fragments arranged by size: 2495 1451 1427 936 635 486 382 186 83 75 53 BsaWI w'CCGG_w Cuts at: 2463 4932 6642 7040 7314 7461 7872 2463 Size: 2469 1710 398 274 147 411 2800 Fragments arranged by size: 2800 2469 1710 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3780 3810 5223 5253 1178 Size: 30 165 30 2377 30 1413 30 4134 Fragments arranged by size: 4134 2377 1413 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2341 2486 5011 1186 Size: 859 296 145 2525 4384 Fragments arranged by size: 4384 2525 859 296 145 BseYI C'CCAG_C Cuts at: 2132 4748 4880 5489 7412 7908 2132 Size: 2616 132 609 1923 496 2433 Fragments arranged by size: 2616 2433 1923 609 496 132 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2295 4037 4156 4631 4889 1163 Size: 573 559 1742 119 475 258 4483 Fragments arranged by size: 4483 1742 573 559 475 258 119 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2323 5011 5659 6420 7448 1540 Size: 275 186 322 2688 648 761 1028 2301 Fragments arranged by size: 2688 2301 1028 761 648 322 275 186 BsiHKAI G_wGCw'C Cuts at: 2621 3555 3762 4680 4686 5079 6270 6355 7426 Size: 934 207 918 6 393 1191 85 1071 Cuts at: 7426 2621 Size: 3404 Fragments arranged by size: 3404 1191 1071 934 918 393 207 85 6 BsmI GAATG_Cn' Cuts at: 3057 3199 4891 5720 5813 3057 Size: 142 1692 829 93 5453 Fragments arranged by size: 5453 1692 829 142 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2323 2323 Size: 8209 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2200 2281 2288 2387 2621 3555 Size: 399 283 127 81 7 99 234 934 Cuts at: 3555 3762 4382 4680 4686 4763 5079 5497 5528 Size: 207 620 298 6 77 316 418 31 Cuts at: 5528 6270 6355 7426 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 934 742 620 418 399 316 298 283 234 207 127 99 85 81 77 31 7 6 BspEI T'CCGG_A Cuts at: 2463 7872 2463 Size: 5409 2800 BspHI T'CATG_A Cuts at: 3399 3939 6098 7828 3399 Size: 540 2159 1730 3780 Fragments arranged by size: 3780 2159 1730 540 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4603 4861 5564 844 Size: 3759 258 703 3489 Fragments arranged by size: 3759 3489 703 258 BsrBI CCG'CTC Cuts at: 1394 1823 2275 2337 2342 2437 3969 4117 6096 Size: 429 452 62 5 95 1532 148 1979 Cuts at: 6096 1394 Size: 3507 Fragments arranged by size: 3507 1979 1532 452 429 148 95 62 5 BsrDI GCAATG_nn' Cuts at: 65 1248 3624 4225 6704 6878 65 Size: 1183 2376 601 2479 174 1396 Fragments arranged by size: 2479 2376 1396 1183 601 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2021 2060 2808 4234 6853 7040 1700 Size: 110 211 39 748 1426 2619 187 2869 Fragments arranged by size: 2869 2619 1426 748 211 187 110 39 BsrGI T'GTAC_A Cuts at: 96 3062 96 Size: 2966 5243 BssHII G'CGCG_C Cuts at: 1925 2049 1925 Size: 124 8085 BssSI C'ACGA_G Cuts at: 6269 7281 6269 Size: 1012 7197 BstAPI GCAn_nnn'nTGC Cuts at: 794 3559 5501 794 Size: 2765 1942 3502 Fragments arranged by size: 3502 2765 1942 BstBI TT'CG_AA Cuts at: 7013 7013 Size: 8209 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8209 BstXI CCAn_nnnn'nTGG Cuts at: 2546 3019 4756 2546 Size: 473 1737 5999 Fragments arranged by size: 5999 1737 473 BstYI r'GATC_y Cuts at: 1972 3532 3744 3857 5125 5303 5483 5539 5906 Size: 1560 212 113 1268 178 180 56 367 Cuts at: 5906 6291 6308 7760 7846 7858 8204 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1560 1452 1268 385 367 346 212 180 178 113 86 56 17 12 Bsu36I CC'TnA_GG Cuts at: 3387 3387 Size: 8209 BtgI C'CryG_G Cuts at: 513 1065 2004 2298 2532 4394 4554 513 Size: 552 939 294 234 1862 160 4168 Fragments arranged by size: 4168 1862 939 552 294 234 160 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4533 4897 487 Size: 683 56 3307 364 3799 Fragments arranged by size: 3799 3307 683 364 56 BtsI GCAGTG_nn' Cuts at: 3553 4359 4743 5094 5355 5725 6519 6539 3553 Size: 806 384 351 261 370 794 20 5223 Fragments arranged by size: 5223 806 794 384 370 351 261 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8209 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8174 DraI TTT'AAA Cuts at: 5371 5513 5371 Size: 142 8067 DraIII CAC_nnn'GTG Cuts at: 3338 3338 Size: 8209 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4643 7216 809 Size: 507 3327 2573 1802 Fragments arranged by size: 3327 2573 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2186 2320 2548 Size: 54 1425 50 275 18 356 134 228 Cuts at: 2548 3164 4222 5656 6545 8174 8 Size: 616 1058 1434 889 1629 43 Fragments arranged by size: 1629 1434 1425 1058 889 616 356 275 228 134 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2320 5656 1537 Size: 275 508 3336 4090 Fragments arranged by size: 4090 3336 508 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3721 3750 3921 4023 4470 4698 4944 6139 Size: 2368 29 171 102 447 228 246 1195 Cuts at: 6139 1353 Size: 3423 Fragments arranged by size: 3423 2368 1195 447 246 228 171 102 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5451 6796 7170 7316 387 Size: 1064 138 3862 1345 374 146 1280 Fragments arranged by size: 3862 1345 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2619 2619 Size: 8209 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2291 3611 3702 3757 3942 3943 4816 Size: 393 659 1320 91 55 185 1 873 Cuts at: 4816 4827 5154 5183 5243 6272 6869 7656 7924 Size: 11 327 29 60 1029 597 787 268 Cuts at: 7924 1239 Size: 1524 Fragments arranged by size: 1524 1320 1029 873 787 659 597 393 327 268 185 91 60 55 29 11 1 EcoNI CCTnn'n_nnAGG Cuts at: 3090 3187 3413 3090 Size: 97 226 7886 Fragments arranged by size: 7886 226 97 EcoO109I rG'GnC_Cy Cuts at: 2440 3383 3544 2440 Size: 943 161 7105 Fragments arranged by size: 7105 943 161 EcoRI G'AATT_C Cuts at: 4928 5518 5896 4928 Size: 590 378 7241 Fragments arranged by size: 7241 590 378 EcoRV GAT'ATC Cuts at: 5172 5172 Size: 8209 FspI TGC'GCA Cuts at: 6715 6715 Size: 8209 HaeII r_GCGC'y Cuts at: 1678 2022 2264 3524 4644 7356 1678 Size: 344 242 1260 1120 2712 2531 Fragments arranged by size: 2712 2531 1260 1120 344 242 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3095 3127 3986 4018 4437 Size: 32 135 32 1523 32 859 32 419 Cuts at: 4437 4469 5156 5188 5223 5229 5255 5261 6850 Size: 32 687 32 35 6 26 6 1589 Cuts at: 6850 6882 6924 6956 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1589 1523 859 687 419 135 42 35 32 32 32 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 2634 5151 5553 5799 669 Size: 988 281 696 2517 402 246 3079 Fragments arranged by size: 3079 2517 988 696 402 281 246 HindIII A'AGCT_T Cuts at: 748 2830 3212 748 Size: 2082 382 5745 Fragments arranged by size: 5745 2082 382 HpaI GTT'AAC Cuts at: 5799 5799 Size: 8209 KasI G'GCGC_C Cuts at: 2018 3520 2018 Size: 1502 6707 KpnI G_GTAC'C Cuts at: 5534 5534 Size: 8209 MfeI C'AATT_G Cuts at: 5505 5808 5505 Size: 303 7906 MluI A'CGCG_T Cuts at: 8085 8085 Size: 8209 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2360 5315 7323 7507 1305 Size: 1055 2955 2008 184 2007 Fragments arranged by size: 2955 2008 2007 1055 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2550 4224 10 Size: 54 1425 343 718 1674 3995 Fragments arranged by size: 3995 1674 1425 718 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3656 3704 3944 4218 4460 4505 4772 Size: 648 2490 48 240 274 242 45 267 Cuts at: 4772 4865 5066 5315 6167 6526 6685 518 Size: 93 201 249 852 359 159 2042 Fragments arranged by size: 2490 2042 852 648 359 274 267 249 242 240 201 159 93 48 45 MspA1I CmG'CkG Cuts at: 1606 3148 3281 3982 4051 4396 4436 4492 4880 Size: 1542 133 701 69 345 40 56 388 Cuts at: 4880 5132 5267 5625 6302 7450 7695 1606 Size: 252 135 358 677 1148 245 2120 Fragments arranged by size: 2120 1542 1148 701 677 388 358 345 252 245 135 133 69 56 40 NaeI GCC'GGC Cuts at: 2023 2062 4236 2023 Size: 39 2174 5996 Fragments arranged by size: 5996 2174 39 NarI GG'CG_CC Cuts at: 2019 3521 2019 Size: 1502 6707 NcoI C'CATG_G Cuts at: 513 1065 2004 2298 2532 4554 513 Size: 552 939 294 234 2022 4168 Fragments arranged by size: 4168 2022 939 552 294 234 NdeI CA'TA_TG Cuts at: 387 387 Size: 8209 NgoMIV G'CCGG_C Cuts at: 2021 2060 4234 2021 Size: 39 2174 5996 Fragments arranged by size: 5996 2174 39 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8209 NotI GC'GGCC_GC Cuts at: 5656 5656 Size: 8209 NsiI A_TGCA'T Cuts at: 3199 3246 3199 Size: 47 8162 NspI r_CATG'y Cuts at: 1132 1229 3244 3248 3709 4074 4661 5567 7112 Size: 97 2015 4 461 365 587 906 1545 Cuts at: 7112 1132 Size: 2229 Fragments arranged by size: 2229 2015 1545 906 587 461 365 97 4 PasI CC'CwG_GG Cuts at: 1348 4382 1348 Size: 3034 5175 PciI A'CATG_T Cuts at: 4657 7108 4657 Size: 2451 5758 PflMI CCAn_nnn'nTGG Cuts at: 1108 2533 1108 Size: 1425 6784 PmeI GTTT'AAAC Cuts at: 5513 5513 Size: 8209 PmlI CAC'GTG Cuts at: 3335 4681 3335 Size: 1346 6863 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6278 6310 7791 7823 6278 Size: 32 1481 32 6664 Fragments arranged by size: 6664 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2440 3544 2440 Size: 1104 7105 PshAI GACnn'nnGTC Cuts at: 1613 4623 5007 1613 Size: 3010 384 4815 Fragments arranged by size: 4815 3010 384 PsiI TTA'TAA Cuts at: 5779 5779 Size: 8209 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3661 3693 3661 Size: 32 8177 PstI C_TGCA'G Cuts at: 830 3519 5561 830 Size: 2689 2042 3478 Fragments arranged by size: 3478 2689 2042 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8209 PvuII CAG'CTG Cuts at: 3148 3982 4051 4880 5132 3148 Size: 834 69 829 252 6225 Fragments arranged by size: 6225 834 829 252 69 SacI G_AGCT'C Cuts at: 2621 2621 Size: 8209 SacII CC_GC'GG Cuts at: 4397 4397 Size: 8209 SalI G'TCGA_C Cuts at: 1936 5551 1936 Size: 3615 4594 SanDI GG'GwC_CC Cuts at: 2440 3544 2440 Size: 1104 7105 SapI GCTCTTCn'nnn_ Cuts at: 3750 3750 Size: 8209 SbfI CC_TGCA'GG Cuts at: 5561 5561 Size: 8209 ScaI AGT'ACT Cuts at: 4303 6457 8169 4303 Size: 2154 1712 4343 Fragments arranged by size: 4343 2154 1712 SexAI A'CCwGG_T Cuts at: 5416 5416 Size: 8209 SfcI C'TryA_G Cuts at: 826 1045 3515 4036 5557 6692 7373 7564 826 Size: 219 2470 521 1521 1135 681 191 1471 Fragments arranged by size: 2470 1521 1471 1135 681 521 219 191 SfoI GGC'GCC Cuts at: 2020 3522 2020 Size: 1502 6707 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8209 SgrAI Cr'CCGG_yG Cuts at: 1700 2021 2060 1700 Size: 321 39 7849 Fragments arranged by size: 7849 321 39 SmaI CCC'GGG Cuts at: 1872 2091 2251 2390 5536 1872 Size: 219 160 139 3146 4545 Fragments arranged by size: 4545 3146 219 160 139 SmlI C'TyrA_G Cuts at: 820 1017 1943 3005 6313 7214 7476 820 Size: 197 926 1062 3308 901 262 1553 Fragments arranged by size: 3308 1553 1062 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6000 493 Size: 5507 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8209 SphI G_CATG'C Cuts at: 1132 5567 1132 Size: 4435 3774 SspI AAT'ATT Cuts at: 5 52 6133 5 Size: 47 6081 2081 Fragments arranged by size: 6081 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8209 StyI C'CwwG_G Cuts at: 513 1065 2004 2162 2298 2532 3234 3357 4043 Size: 552 939 158 136 234 702 123 686 Cuts at: 4043 4554 4671 4812 5648 513 Size: 511 117 141 836 3074 Fragments arranged by size: 3074 939 836 702 686 552 511 234 158 141 136 123 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2271 3902 4997 6236 6253 6406 6591 Size: 1255 691 1631 1095 1239 17 153 185 Cuts at: 6591 7036 325 Size: 445 1498 Fragments arranged by size: 1631 1498 1255 1239 1095 691 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3062 4301 4356 Size: 274 80 33 51 1443 1085 1239 55 Cuts at: 4356 4650 5209 6455 8167 96 Size: 294 559 1246 1712 138 Fragments arranged by size: 1712 1443 1246 1239 1085 559 294 274 138 80 55 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3871 3903 5032 5064 8169 8201 3871 Size: 32 1129 32 3105 32 3879 Fragments arranged by size: 3879 3105 1129 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8209 XbaI T'CTAG_A Cuts at: 5545 5545 Size: 8209 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4511 4511 Size: 8209 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8209 XmaI C'CCGG_G Cuts at: 1870 2089 2249 2388 5534 1870 Size: 219 160 139 3146 4545 Fragments arranged by size: 4545 3146 219 160 139 XmnI GAAnn'nnTTC Cuts at: 3476 6338 3476 Size: 2862 5347 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6017 276 Size: 53 83 186 936 4483 2468 Fragments arranged by size: 4483 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI BaeI BbvCI BplI Bpu10I BsiWI BstZ17I FalI FseI FspAI NruI PacI PfoI PspOMI PspXI RsrII SfiI SrfI SwaI