Restriction map for
pFN21AB3827
(Linear) (Six-base) MAPSORT of: KIBB3827.seq Check: 1337 from: 1 to: 3504
KIBB3827.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2598 3504
Size: 2598 906
AccI GT'mk_AC
Cuts at: 0 1337 3504
Size: 1337 2167
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1606 1697 1938 2811 2822 3238 3504
Size: 1606 91 241 873 11 416 266
Fragments arranged by size:
1606 873 416 266 241 91 11
AfeI AGC'GCT
Cuts at: 0 257 3504
Size: 257 3247
AflIII A'CryG_T
Cuts at: 0 1172 1301 1327 2568 2652 3504
Size: 1172 129 26 1241 84 852
Fragments arranged by size:
1241 1172 852 129 84 26
AhdI GACnn_n'nnGTC
Cuts at: 0 2446 3504
Size: 2446 1058
AleI CACnn'nnGTG
Cuts at: 0 2767 3504
Size: 2767 737
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3089 3121 3504
Size: 3089 32 383
Fragments arranged by size:
3089 383 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1499 1690 2226 3504
Size: 1499 191 536 1278
Fragments arranged by size:
1499 1278 536 191
ApaLI G'TGCA_C
Cuts at: 0 1546 2677 3504
Size: 1546 1131 827
Fragments arranged by size:
1546 1131 827
ApoI r'AATT_y
Cuts at: 0 901 2923 3504
Size: 901 2022 581
Fragments arranged by size:
2022 901 581
AvaI C'yCGr_G
Cuts at: 0 84 244 275 383 425 3504
Size: 84 160 31 108 42 3079
Fragments arranged by size:
3079 160 108 84 42 31
AvrII C'CTAG_G
Cuts at: 0 157 3504
Size: 157 3347
BamHI G'GATC_C
Cuts at: 0 3120 3504
Size: 3120 384
BanI G'GyrC_C
Cuts at: 0 13 1515 2755 3504
Size: 13 1502 1240 749
Fragments arranged by size:
1502 1240 749 13
BanII G_rGCy'C
Cuts at: 0 68 195 276 382 616 3504
Size: 68 127 81 106 234 2888
Fragments arranged by size:
2888 234 127 106 81 68
BbeI G_GCGC'C
Cuts at: 0 17 1519 3504
Size: 17 1502 1985
Fragments arranged by size:
1985 1502 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 857 1474 3504
Size: 857 617 2030
Fragments arranged by size:
2030 857 617
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2715 2749 3504
Size: 2715 34 755
Fragments arranged by size:
2715 755 34
BclI T'GATC_A
Cuts at: 0 767 2801 3504
Size: 767 2034 703
Fragments arranged by size:
2034 767 703
BfrBI ATG'CAT
Cuts at: 0 1192 1239 3504
Size: 1192 47 2265
Fragments arranged by size:
2265 1192 47
BglI GCCn_nnn'nGGC
Cuts at: 0 1385 2753 3504
Size: 1385 1368 751
Fragments arranged by size:
1385 1368 751
BglII A'GATC_T
Cuts at: 0 1739 1852 3504
Size: 1739 113 1652
Fragments arranged by size:
1739 1652 113
BlpI GC'TnA_GC
Cuts at: 0 40 3504
Size: 40 3464
Bme1580I G_kGCm'C
Cuts at: 0 283 1550 2377 2681 2758 3492 3504
Size: 283 1267 827 304 77 734 12
Fragments arranged by size:
1267 827 734 304 283 77 12
BmgBI CAC'GTC
Cuts at: 0 2571 3504
Size: 2571 933
BmrI ACTGGGnnnn_n'
Cuts at: 0 293 2309 3504
Size: 293 2016 1195
Fragments arranged by size:
2016 1195 293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 1752 1937 3149 3178 3504
Size: 286 1466 185 1212 29 326
Fragments arranged by size:
1466 1212 326 286 185 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1021 3504
Size: 1021 2483
BsaI GGTCTCn'nnnn_
Cuts at: 0 1596 2874 2936 3504
Size: 1596 1278 62 568
Fragments arranged by size:
1596 1278 568 62
BsaAI yAC'GTr
Cuts at: 0 1173 1304 1330 2676 3504
Size: 1173 131 26 1346 828
Fragments arranged by size:
1346 1173 828 131 26
BsaBI GATnn'nnATC
Cuts at: 0 1259 3504
Size: 1259 2245
BsaHI Gr'CG_yC
Cuts at: 0 14 89 1516 3504
Size: 14 75 1427 1988
Fragments arranged by size:
1988 1427 75 14
BsaWI w'CCGG_w
Cuts at: 0 458 2927 3504
Size: 458 2469 577
Fragments arranged by size:
2469 577 458
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1775 1805 3218 3248 3504
Size: 1775 30 1413 30 256
Fragments arranged by size:
1775 1413 256 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 336 481 3006 3504
Size: 40 296 145 2525 498
Fragments arranged by size:
2525 498 296 145 40
BseYI C'CCAG_C
Cuts at: 0 127 2743 2875 3484 3504
Size: 127 2616 132 609 20
Fragments arranged by size:
2616 609 132 127 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 2032 2151 2626 2884 3504
Size: 290 1742 119 475 258 620
Fragments arranged by size:
1742 620 475 290 258 119
BsiEI CG_ry'CG
Cuts at: 0 318 3006 3504
Size: 318 2688 498
Fragments arranged by size:
2688 498 318
BsiHKAI G_wGCw'C
Cuts at: 0 616 1550 1757 2675 2681 3074 3504
Size: 616 934 207 918 6 393 430
Fragments arranged by size:
934 918 616 430 393 207 6
BsmI GAATG_Cn'
Cuts at: 0 1052 1194 2886 3504
Size: 1052 142 1692 618
Fragments arranged by size:
1692 1052 618 142
BsmBI CGTCTCn'nnnn_
Cuts at: 0 318 3504
Size: 318 3186
Bsp1286I G_dGCh'C
Cuts at: 0 68 195 276 283 382 616 1550 1757
Size: 68 127 81 7 99 234 934 207
Cuts at: 1757 2377 2675 2681 2758 3074 3492 3504
Size: 620 298 6 77 316 418 12
Fragments arranged by size:
934 620 418 316 298 234 207 127
99 81 77 68 12 7 6
BspEI T'CCGG_A
Cuts at: 0 458 3504
Size: 458 3046
BspHI T'CATG_A
Cuts at: 0 1394 1934 3504
Size: 1394 540 1570
Fragments arranged by size:
1570 1394 540
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2598 2856 3504
Size: 2598 258 648
Fragments arranged by size:
2598 648 258
BsrBI CCG'CTC
Cuts at: 0 270 332 337 432 1964 2112 3504
Size: 270 62 5 95 1532 148 1392
Fragments arranged by size:
1532 1392 270 148 95 62 5
BsrDI GCAATG_nn'
Cuts at: 0 1619 2220 3504
Size: 1619 601 1284
Fragments arranged by size:
1619 1284 601
BsrFI r'CCGG_y
Cuts at: 0 16 55 803 2229 3504
Size: 16 39 748 1426 1275
Fragments arranged by size:
1426 1275 748 39 16
BsrGI T'GTAC_A
Cuts at: 0 1057 3504
Size: 1057 2447
BssHII G'CGCG_C
Cuts at: 0 44 3504
Size: 44 3460
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1554 3496 3504
Size: 1554 1942 8
Fragments arranged by size:
1942 1554 8
BstXI CCAn_nnnn'nTGG
Cuts at: 0 541 1014 2751 3504
Size: 541 473 1737 753
Fragments arranged by size:
1737 753 541 473
BstYI r'GATC_y
Cuts at: 0 1527 1739 1852 3120 3298 3478 3504
Size: 1527 212 113 1268 178 180 26
Fragments arranged by size:
1527 1268 212 180 178 113 26
Bsu36I CC'TnA_GG
Cuts at: 0 1382 3504
Size: 1382 2122
BtgI C'CryG_G
Cuts at: 0 293 527 2389 2549 3504
Size: 293 234 1862 160 955
Fragments arranged by size:
1862 955 293 234 160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2528 2892 3504
Size: 2528 364 612
Fragments arranged by size:
2528 612 364
BtsI GCAGTG_nn'
Cuts at: 0 1548 2354 2738 3089 3350 3504
Size: 1548 806 384 351 261 154
Fragments arranged by size:
1548 806 384 351 261 154
DraI TTT'AAA
Cuts at: 0 3366 3504
Size: 3366 138
DraIII CAC_nnn'GTG
Cuts at: 0 1333 3504
Size: 1333 2171
DrdI GACnn_nn'nnGTC
Cuts at: 0 2638 3504
Size: 2638 866
EaeI y'GGCC_r
Cuts at: 0 181 315 543 1159 2217 3504
Size: 181 134 228 616 1058 1287
Fragments arranged by size:
1287 1058 616 228 181 134
EagI C'GGCC_G
Cuts at: 0 315 3504
Size: 315 3189
EarI CTCTTCn'nnn_
Cuts at: 0 1716 1745 1916 2018 2465 2693 2939 3504
Size: 1716 29 171 102 447 228 246 565
Fragments arranged by size:
1716 565 447 246 228 171 102 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3446 3504
Size: 3446 58
EcoICRI GAG'CTC
Cuts at: 0 614 3504
Size: 614 2890
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 1606 1697 1752 1937 1938 2811 2822
Size: 286 1320 91 55 185 1 873 11
Cuts at: 2822 3149 3178 3238 3504
Size: 327 29 60 266
Fragments arranged by size:
1320 873 327 286 266 185 91 60
55 29 11 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1085 1182 1408 3504
Size: 1085 97 226 2096
Fragments arranged by size:
2096 1085 226 97
EcoO109I rG'GnC_Cy
Cuts at: 0 435 1378 1539 3504
Size: 435 943 161 1965
Fragments arranged by size:
1965 943 435 161
EcoRI G'AATT_C
Cuts at: 0 2923 3504
Size: 2923 581
EcoRV GAT'ATC
Cuts at: 0 3167 3504
Size: 3167 337
HaeII r_GCGC'y
Cuts at: 0 17 259 1519 2639 3504
Size: 17 242 1260 1120 865
Fragments arranged by size:
1260 1120 865 242 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1090 1122 1981 2013 2432 2464 3151 3183
Size: 1090 32 859 32 419 32 687 32
Cuts at: 3183 3218 3224 3250 3256 3504
Size: 35 6 26 6 248
Fragments arranged by size:
1090 859 687 419 248 35 32 32
32 32 26 6 6
HincII GTy'rAC
Cuts at: 0 629 3146 3504
Size: 629 2517 358
Fragments arranged by size:
2517 629 358
HindIII A'AGCT_T
Cuts at: 0 825 1207 3504
Size: 825 382 2297
Fragments arranged by size:
2297 825 382
KasI G'GCGC_C
Cuts at: 0 13 1515 3504
Size: 13 1502 1989
Fragments arranged by size:
1989 1502 13
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 3310 3504
Size: 355 2955 194
Fragments arranged by size:
2955 355 194
MscI TGG'CCA
Cuts at: 0 545 2219 3504
Size: 545 1674 1285
Fragments arranged by size:
1674 1285 545
MslI CAynn'nnrTG
Cuts at: 0 1651 1699 1939 2213 2455 2500 2767 2860
Size: 1651 48 240 274 242 45 267 93
Cuts at: 2860 3061 3310 3504
Size: 201 249 194
Fragments arranged by size:
1651 274 267 249 242 240 201 194
93 48 45
MspA1I CmG'CkG
Cuts at: 0 1143 1276 1977 2046 2391 2431 2487 2875
Size: 1143 133 701 69 345 40 56 388
Cuts at: 2875 3127 3262 3504
Size: 252 135 242
Fragments arranged by size:
1143 701 388 345 252 242 135 133
69 56 40
NaeI GCC'GGC
Cuts at: 0 18 57 2231 3504
Size: 18 39 2174 1273
Fragments arranged by size:
2174 1273 39 18
NarI GG'CG_CC
Cuts at: 0 14 1516 3504
Size: 14 1502 1988
Fragments arranged by size:
1988 1502 14
NcoI C'CATG_G
Cuts at: 0 293 527 2549 3504
Size: 293 234 2022 955
Fragments arranged by size:
2022 955 293 234
NgoMIV G'CCGG_C
Cuts at: 0 16 55 2229 3504
Size: 16 39 2174 1275
Fragments arranged by size:
2174 1275 39 16
NsiI A_TGCA'T
Cuts at: 0 1194 1241 3504
Size: 1194 47 2263
Fragments arranged by size:
2263 1194 47
NspI r_CATG'y
Cuts at: 0 1239 1243 1704 2069 2656 3504
Size: 1239 4 461 365 587 848
Fragments arranged by size:
1239 848 587 461 365 4
PasI CC'CwG_GG
Cuts at: 0 2377 3504
Size: 2377 1127
PciI A'CATG_T
Cuts at: 0 2652 3504
Size: 2652 852
PflMI CCAn_nnn'nTGG
Cuts at: 0 528 3504
Size: 528 2976
PmlI CAC'GTG
Cuts at: 0 1330 2676 3504
Size: 1330 1346 828
Fragments arranged by size:
1346 1330 828
PpuMI rG'GwC_Cy
Cuts at: 0 435 1539 3504
Size: 435 1104 1965
Fragments arranged by size:
1965 1104 435
PshAI GACnn'nnGTC
Cuts at: 0 2618 3002 3504
Size: 2618 384 502
Fragments arranged by size:
2618 502 384
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1656 1688 3504
Size: 1656 32 1816
Fragments arranged by size:
1816 1656 32
PstI C_TGCA'G
Cuts at: 0 1514 3504
Size: 1514 1990
PvuII CAG'CTG
Cuts at: 0 1143 1977 2046 2875 3127 3504
Size: 1143 834 69 829 252 377
Fragments arranged by size:
1143 834 829 377 252 69
SacI G_AGCT'C
Cuts at: 0 616 3504
Size: 616 2888
SacII CC_GC'GG
Cuts at: 0 2392 3504
Size: 2392 1112
SanDI GG'GwC_CC
Cuts at: 0 435 1539 3504
Size: 435 1104 1965
Fragments arranged by size:
1965 1104 435
SapI GCTCTTCn'nnn_
Cuts at: 0 1745 3504
Size: 1745 1759
ScaI AGT'ACT
Cuts at: 0 2298 3504
Size: 2298 1206
SexAI A'CCwGG_T
Cuts at: 0 3411 3504
Size: 3411 93
SfcI C'TryA_G
Cuts at: 0 1510 2031 3504
Size: 1510 521 1473
Fragments arranged by size:
1510 1473 521
SfoI GGC'GCC
Cuts at: 0 15 1517 3504
Size: 15 1502 1987
Fragments arranged by size:
1987 1502 15
SgrAI Cr'CCGG_yG
Cuts at: 0 16 55 3504
Size: 16 39 3449
Fragments arranged by size:
3449 39 16
SmaI CCC'GGG
Cuts at: 0 86 246 385 3504
Size: 86 160 139 3119
Fragments arranged by size:
3119 160 139 86
SmlI C'TyrA_G
Cuts at: 0 1000 3504
Size: 1000 2504
StyI C'CwwG_G
Cuts at: 0 157 293 527 1229 1352 2038 2549 2666
Size: 157 136 234 702 123 686 511 117
Cuts at: 2666 2807 3504
Size: 141 697
Fragments arranged by size:
702 697 686 511 234 157 141 136
123 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 266 1897 2992 3504
Size: 266 1631 1095 512
Fragments arranged by size:
1631 1095 512 266
TatI w'GTAC_w
Cuts at: 0 1057 2296 2351 2645 3204 3504
Size: 1057 1239 55 294 559 300
Fragments arranged by size:
1239 1057 559 300 294 55
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1866 1898 3027 3059 3504
Size: 1866 32 1129 32 445
Fragments arranged by size:
1866 1129 445 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2506 3504
Size: 2506 998
XmaI C'CCGG_G
Cuts at: 0 84 244 383 3504
Size: 84 160 139 3121
Fragments arranged by size:
3121 160 139 84
XmnI GAAnn'nnTTC
Cuts at: 0 1471 3504
Size: 1471 2033
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AloI
AlwNI ApaLI ApoI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII
Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PmlI PpuMI PshAI PsrI PstI PvuII SacI
SacII SanDI SapI ScaI SexAI SfcI SfoI SgrAI
SmaI SmlI StyI TaqII TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AlfI ApaI AscI
AseI AsiSI BaeI BbvCI BciVI BmtI BplI Bpu10I
BsiWI BssSI BstBI BstEII BstZ17I ClaI CspCI FalI
FseI FspI FspAI HpaI KpnI MfeI MluI NdeI
NheI NotI NruI PacI PfoI PmeI PpiI PsiI
PspOMI PspXI PvuI RsrII SalI SbfI SfiI SgfI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XhoI ZraI