Restriction map for
pFN21AB3818
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# NO CUT ENZYMES ( 128 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BbeI BbrPI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoT14I EcoT22I FseI FspI HindIII
HpaI KasI KpnI KspI MaeIII
MamI MfeI MluI MroI MseI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tsp45I XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 382 GACGTC GACGT^C
AflIII 9 ACGTGT A^C[GA][CT]GT
AspHI 604 GTGCTC G[AT]GC[AT]^C
AspI 853 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 455 TGGCCA TGG^CCA
BanI 76 GGTGCC G^G[CT][GA]CC
BbsI 126 GAAGAC GAAGAC(2/6)
BbvCI 217 GCTGAGG CCTCAGC(-5/-2)
BcgI 567 GCAAAGCCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 335 TGATCA T^GATCA
BfaI 260 CTAG C^TAG
BpuAI 126 GAAGAC GAAGAC(2/6)
BsaI 1194 GGTCTC GGTCTC(1/5)
BsiEI 1110 CGACCG CG[GA][CT]^CG
BsiHKAI 604 GTGCTC G[AT]GC[AT]^C
BsmBI 1161 CGTCTC CGTCTC(1/5)
EcoRI 739 GAATTC G^AATTC
EcoRV 64 GATATC GAT^ATC
FbaI 335 TGATCA T^GATCA
HaeII 662 AGCGCC [GA]GCGC^[CT]
HgaI 472 GACGC GACGC(5/10)
HinfI 897 GAATC G^A[ACGT]TC
MaeI 260 CTAG C^TAG
MluNI 455 TGGCCA TGG^CCA
MscI 455 TGGCCA TGG^CCA
MslI 1141 CACAGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 142 CAGCTG C[AC]G^C[GT]G
NaeI 1071 GCCGGC GCC^GGC
NgoMIV 1071 GCCGGC G^CCGGC
NspI 872 ACATGC [GA]CATG^[CT]
PaeR7I 265 CTCGAG C^TCGAG
PflFI 853 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 200 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 142 CAGCTG CAG^CTG
SalI 1108 GTCGAC G^TCGAC
SapI 813 GCTCTTC GCTCTTC(1/4)
SexAI 428 ACCTGGT A^CC[AT]GGT
TfiI 897 GAATC G^A[AT]TC
Tth111I 853 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 200 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 265 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 433 GTCTAC GT^[AC][GT]AC
1108 GTCGAC
AcyI 382 GACGTC G[GA]^CG[CT]C
472 GACGCC
BamHI 191 GGATCC G^GATCC
1202 GGATCC
BpmI 115 CTGGAG CTGGAG(16/14)
586 CTGGAG
BsaAI 8 TACGTG [CT]AC^GT[GA]
601 TACGTG
BsaHI 382 GACGTC G[GA]^CG[CT]C
472 GACGCC
BsmAI 1162 GTCTC GTCTC(1/5)
1195 GTCTC
BsrI 866 ACTGG ACTGG(1/-1)
1180 ACTGG
BstXI 107 CCATCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1068 CCAGCCGGCTGG
BstYI 191 GGATCC [GA]^GATC[CT]
1202 GGATCC
CfoI 663 GCGC GCG^C
1150 GCGC
DdeI 218 CTGAG C^T[ACGT]AG
1170 CTCAG
DsaI 998 CCACGG C^C[GA][CT]GG
1040 CCGTGG
EaeI 323 TGGCCG [CT]^GGCC[GA]
455 TGGCCA
EarI 814 CTCTTC CTCTTC(1/4)
826 CTCTTC
HhaI 663 GCGC GCG^C
1150 GCGC
Hin1I 382 GACGTC G[GA]^CG[CT]C
472 GACGCC
HinP1I 663 GCGC G^CGC
1150 GCGC
HincII 699 GTCAAC GT[CT]^[GA]AC
1108 GTCGAC
HindII 699 GTCAAC GT[CT]^[GA]AC
1108 GTCGAC
HphI 94 GGTGA GGTGA(8/7)
1125 GGTGA
Ksp632I 814 CTCTTC CTCTTC(1/4)
826 CTCTTC
MflI 191 GGATCC [GA]^GATC[CT]
1202 GGATCC
SmlI 265 CTCGAG C^T[CT][GA]AG
304 CTCAAG
XhoII 191 GGATCC [GA]^GATC[CT]
1202 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaI AvaII BanII
BbvI BcnI BmyI BsaJI BseRI
BsgI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BssKI BstF5I
BstNI BstUI Cac8I Cfr10I Cfr13I
DpnII DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HpaII
ItaI MaeII MboI MboII MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI Tsp509I TspRI TthHB8I