(Circular) (Six-base) MAPSORT of: pFN21AB3818.seq Check: 9298 from: 1 to: 5953 pFN21AB3818 5953 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2391 3763 278 Size: 53 83 186 936 855 1372 2468 Fragments arranged by size: 2468 1372 936 855 186 83 53 AccI GT'mk_AC Cuts at: 1937 2439 3114 3296 1937 Size: 502 675 182 4594 Fragments arranged by size: 4594 675 502 182 Acc65I G'GTAC_C Cuts at: 3274 3274 Size: 5953 AclI AA'CG_TT Cuts at: 1563 4080 4453 1563 Size: 2517 373 3063 Fragments arranged by size: 3063 2517 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4016 5400 5668 1632 Size: 2384 1384 268 1917 Fragments arranged by size: 2384 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5756 AflIII A'CryG_T Cuts at: 1203 2014 4852 5829 1203 Size: 811 2838 977 1327 Fragments arranged by size: 2838 1327 977 811 AgeI A'CCGG_T Cuts at: 4784 4784 Size: 5953 AhdI GACnn_n'nnGTC Cuts at: 4682 4682 Size: 5953 AleI CACnn'nnGTG Cuts at: 1166 3150 1166 Size: 1984 3969 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3168 1184 Size: 1984 3969 AlwNI CAG_nnn'CTG Cuts at: 3696 5268 3696 Size: 1572 4381 ApaLI G'TGCA_C Cuts at: 4010 5166 4010 Size: 1156 4797 ApoI r'AATT_y Cuts at: 1493 2300 2744 2885 3262 3488 3640 5749 1493 Size: 807 444 141 377 226 152 2109 1697 Fragments arranged by size: 2109 1697 807 444 377 226 152 141 AseI AT'TA_AT Cuts at: 160 1032 4507 160 Size: 872 3475 1606 Fragments arranged by size: 3475 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 AvaI C'yCGr_G Cuts at: 1870 1943 2270 2906 2979 3245 3278 1870 Size: 73 327 636 73 266 33 4545 Fragments arranged by size: 4545 636 327 266 73 73 33 BamHI G'GATC_C Cuts at: 2196 3207 3283 3650 2196 Size: 1011 76 367 4499 Fragments arranged by size: 4499 1011 367 76 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2081 3274 4729 618 Size: 325 217 627 294 1193 1455 1842 Fragments arranged by size: 1842 1455 1193 627 325 294 217 BanII G_rGCy'C Cuts at: 1391 2633 2652 2986 3045 3272 1391 Size: 1242 19 334 59 227 4072 Fragments arranged by size: 4072 1242 334 227 59 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2138 928 Size: 971 239 4743 Fragments arranged by size: 4743 971 239 BbvCI CC'TCA_GC Cuts at: 2223 2223 Size: 5953 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2561 2595 4144 4178 1903 Size: 34 624 34 1549 34 3678 Fragments arranged by size: 3678 1549 624 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3845 5055 3845 Size: 1210 4743 BclI T'GATC_A Cuts at: 1548 2340 1548 Size: 792 5161 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4564 136 Size: 107 122 71 631 605 2892 1525 Fragments arranged by size: 2892 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5948 5948 Size: 5953 BlpI GC'TnA_GC Cuts at: 3370 3370 Size: 5953 Bme1580I G_kGCm'C Cuts at: 1790 4014 5170 1790 Size: 2224 1156 2573 Fragments arranged by size: 2573 2224 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5953 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3195 3684 4642 Size: 416 53 83 399 24 1778 489 958 Cuts at: 4642 442 Size: 1753 Fragments arranged by size: 1778 1753 958 489 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5953 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2612 4613 1239 Size: 902 471 2001 2579 Fragments arranged by size: 2579 2001 902 471 Bpu10I CC'TnA_GC Cuts at: 2223 2223 Size: 5953 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2294 4078 4943 5241 2294 Size: 1784 865 298 3006 Fragments arranged by size: 3006 1784 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3205 4616 882 Size: 617 1706 1411 2219 Fragments arranged by size: 2219 1706 1411 617 BsaAI yAC'GTr Cuts at: 493 1204 2015 2608 3744 493 Size: 711 811 593 1136 2702 Fragments arranged by size: 2702 1136 811 711 593 BsaBI GATnn'nnATC Cuts at: 5947 5947 Size: 5953 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2388 2478 3760 4142 Size: 53 83 186 936 855 90 1282 382 Cuts at: 4142 4777 275 Size: 635 1451 Fragments arranged by size: 1451 1282 936 855 635 382 186 90 83 53 BsaWI w'CCGG_w Cuts at: 4386 4784 5058 5205 5616 4386 Size: 398 274 147 411 4723 Fragments arranged by size: 4723 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2615 2645 3155 3185 1178 Size: 30 165 30 1212 30 510 30 3946 Fragments arranged by size: 3946 1212 510 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2960 3160 3163 1186 Size: 1774 200 3 3976 Fragments arranged by size: 3976 1774 200 3 BseYI C'CCAG_C Cuts at: 2145 2257 2416 3211 5156 5652 2145 Size: 112 159 795 1945 496 2446 Fragments arranged by size: 2446 1945 795 496 159 112 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 2913 3128 1163 Size: 573 399 778 215 3988 Fragments arranged by size: 3988 778 573 399 215 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3118 3403 4164 5192 1540 Size: 275 186 1117 285 761 1028 2301 Fragments arranged by size: 2301 1117 1028 761 285 275 186 BsiHKAI G_wGCw'C Cuts at: 2613 4014 4099 5170 2613 Size: 1401 85 1071 3396 Fragments arranged by size: 3396 1401 1071 85 BsmI GAATG_Cn' Cuts at: 3464 3557 3464 Size: 93 5860 BsmBI CGTCTCn'nnnn_ Cuts at: 3172 3172 Size: 5953 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2613 2633 2652 2986 3045 3272 4014 Size: 399 823 20 19 334 59 227 742 Cuts at: 4014 4099 5170 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 823 742 399 334 227 85 59 20 19 BspEI T'CCGG_A Cuts at: 5616 5616 Size: 5953 BspHI T'CATG_A Cuts at: 3842 5572 3842 Size: 1730 4223 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3308 844 Size: 2464 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2055 2772 2926 3840 1394 Size: 429 232 717 154 914 3507 Fragments arranged by size: 3507 914 717 429 232 154 BsrDI GCAATG_nn' Cuts at: 65 1248 4448 4622 65 Size: 1183 3200 174 1396 Fragments arranged by size: 3200 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2525 2838 3021 3076 4597 4784 1700 Size: 110 715 313 183 55 1521 187 2869 Fragments arranged by size: 2869 1521 715 313 187 183 110 55 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5953 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5953 BssSI C'ACGA_G Cuts at: 4013 5025 4013 Size: 1012 4941 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5953 BstBI TT'CG_AA Cuts at: 4757 4757 Size: 5953 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5953 BstXI CCAn_nnnn'nTGG Cuts at: 2119 3080 2119 Size: 961 4992 BstYI r'GATC_y Cuts at: 1972 2196 3207 3283 3650 4035 4052 5504 5590 Size: 224 1011 76 367 385 17 1452 86 Cuts at: 5590 5602 5948 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1011 385 367 346 224 86 76 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3003 3045 513 Size: 552 939 999 42 3421 Fragments arranged by size: 3421 999 939 552 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2074 487 Size: 683 56 848 4366 Fragments arranged by size: 4366 848 683 56 BtsI GCAGTG_nn' Cuts at: 3012 3037 3469 4263 4283 3012 Size: 25 432 794 20 4682 Fragments arranged by size: 4682 794 432 25 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5953 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5918 DraI TTT'AAA Cuts at: 3257 3257 Size: 5953 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4960 809 Size: 507 3644 1802 Fragments arranged by size: 3644 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2328 2460 3400 Size: 54 1425 50 275 18 498 132 940 Cuts at: 3400 4289 5918 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 940 889 498 275 132 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3400 1537 Size: 275 1588 4090 Fragments arranged by size: 4090 1588 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2825 2837 3883 1353 Size: 1472 12 1046 3423 Fragments arranged by size: 3423 1472 1046 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4540 4914 5060 387 Size: 1064 138 2951 374 146 1280 Fragments arranged by size: 2951 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2612 4016 4613 5400 5668 1239 Size: 393 509 471 1404 597 787 268 1524 Fragments arranged by size: 1524 1404 787 597 509 471 393 268 EcoO109I rG'GnC_Cy Cuts at: 2058 2261 2275 2719 2058 Size: 203 14 444 5292 Fragments arranged by size: 5292 444 203 14 EcoRI G'AATT_C Cuts at: 2744 3262 3640 2744 Size: 518 378 5057 Fragments arranged by size: 5057 518 378 EcoRV GAT'ATC Cuts at: 2071 2071 Size: 5953 FspI TGC'GCA Cuts at: 4459 4459 Size: 5953 HaeII r_GCGC'y Cuts at: 1678 2671 5100 1678 Size: 993 2429 2531 Fragments arranged by size: 2531 2429 993 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2031 2063 4594 4626 4668 Size: 32 135 32 459 32 2531 32 42 Cuts at: 4668 4700 1373 Size: 32 2626 Fragments arranged by size: 2626 2531 459 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2706 3115 3297 3543 669 Size: 988 281 768 409 182 246 3079 Fragments arranged by size: 3079 988 768 409 281 246 182 HindIII A'AGCT_T Cuts at: 748 748 Size: 5953 HpaI GTT'AAC Cuts at: 3543 3543 Size: 5953 KpnI G_GTAC'C Cuts at: 3278 3278 Size: 5953 MfeI C'AATT_G Cuts at: 3552 3552 Size: 5953 MluI A'CGCG_T Cuts at: 5829 5829 Size: 5953 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5067 5251 1305 Size: 3762 184 2007 Fragments arranged by size: 3762 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2462 10 Size: 54 1425 343 630 3501 Fragments arranged by size: 3501 1425 630 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3150 3911 4270 4429 518 Size: 648 1984 761 359 159 2042 Fragments arranged by size: 2042 1984 761 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2149 3369 4046 5194 5439 1606 Size: 543 1220 677 1148 245 2120 Fragments arranged by size: 2120 1220 1148 677 543 245 NaeI GCC'GGC Cuts at: 3078 3078 Size: 5953 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4462 Fragments arranged by size: 4462 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5953 NgoMIV G'CCGG_C Cuts at: 3076 3076 Size: 5953 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5953 NotI GC'GGCC_GC Cuts at: 3400 3400 Size: 5953 NspI r_CATG'y Cuts at: 1132 1229 2881 3311 4856 1132 Size: 97 1652 430 1545 2229 Fragments arranged by size: 2229 1652 1545 430 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5953 PciI A'CATG_T Cuts at: 4852 4852 Size: 5953 PflMI CCAn_nnn'nTGG Cuts at: 1108 2211 1108 Size: 1103 4850 PfoI T'CCnGG_A Cuts at: 3203 3203 Size: 5953 PmeI GTTT'AAAC Cuts at: 3257 3257 Size: 5953 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2150 2182 2264 2296 4022 4054 5535 5567 2150 Size: 32 82 32 1726 32 1481 32 2536 Fragments arranged by size: 2536 1726 1481 82 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2058 2275 2719 2058 Size: 217 444 5292 Fragments arranged by size: 5292 444 217 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5953 PsiI TTA'TAA Cuts at: 3523 3523 Size: 5953 PspXI vC'TCGA_Gb Cuts at: 2270 2270 Size: 5953 PstI C_TGCA'G Cuts at: 830 3305 830 Size: 2475 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5953 PvuII CAG'CTG Cuts at: 2149 2149 Size: 5953 SalI G'TCGA_C Cuts at: 1936 3113 3295 1936 Size: 1177 182 4594 Fragments arranged by size: 4594 1177 182 SapI GCTCTTCn'nnn_ Cuts at: 2825 2825 Size: 5953 SbfI CC_TGCA'GG Cuts at: 3305 3305 Size: 5953 ScaI AGT'ACT Cuts at: 4201 5913 4201 Size: 1712 4241 SexAI A'CCwGG_T Cuts at: 2433 2433 Size: 5953 SfcI C'TryA_G Cuts at: 826 1045 3301 4436 5117 5308 826 Size: 219 2256 1135 681 191 1471 Fragments arranged by size: 2256 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5953 SmaI CCC'GGG Cuts at: 1872 3280 1872 Size: 1408 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2270 2309 4057 4958 5220 820 Size: 197 926 327 39 1748 901 262 1553 Fragments arranged by size: 1748 1553 926 901 327 262 197 39 SnaBI TAC'GTA Cuts at: 493 3744 493 Size: 3251 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5953 SphI G_CATG'C Cuts at: 1132 3311 1132 Size: 2179 3774 SspI AAT'ATT Cuts at: 5 52 3877 5 Size: 47 3825 2081 Fragments arranged by size: 3825 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5953 StyI C'CwwG_G Cuts at: 513 1065 2004 3392 513 Size: 552 939 1388 3074 Fragments arranged by size: 3074 1388 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2069 2501 3016 3980 3997 4150 4335 Size: 1255 489 432 515 964 17 153 185 Cuts at: 4335 4780 325 Size: 445 1498 Fragments arranged by size: 1498 1255 964 515 489 445 432 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2616 4199 5911 Size: 274 80 33 51 1443 639 1583 1712 Cuts at: 5911 96 Size: 138 Fragments arranged by size: 1712 1583 1443 639 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5913 5945 5913 Size: 32 5921 Tth111I GACn'n_nGTC Cuts at: 1316 2861 1316 Size: 1545 4408 XbaI T'CTAG_A Cuts at: 3289 3289 Size: 5953 XhoI C'TCGA_G Cuts at: 1943 2270 1943 Size: 327 5626 XmaI C'CCGG_G Cuts at: 1870 3278 1870 Size: 1408 4545 XmnI GAAnn'nnTTC Cuts at: 4082 4082 Size: 5953 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2389 3761 276 Size: 53 83 186 936 855 1372 2468 Fragments arranged by size: 2468 1372 936 855 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI