Restriction map for
pFN21AB3818
(Linear) (Six-base) MAPSORT of: KIBB3818.seq Check: 2110 from: 1 to: 1248
KIBB3818.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AatII G_ACGT'C
Cuts at: 0 386 1248
Size: 386 862
AccI GT'mk_AC
Cuts at: 0 434 1109 1248
Size: 434 675 139
Fragments arranged by size:
675 434 139
AflIII A'CryG_T
Cuts at: 0 9 1248
Size: 9 1239
AleI CACnn'nnGTG
Cuts at: 0 1145 1248
Size: 1145 103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1163 1248
Size: 1163 85
ApoI r'AATT_y
Cuts at: 0 295 739 880 1248
Size: 295 444 141 368
Fragments arranged by size:
444 368 295 141
AvaI C'yCGr_G
Cuts at: 0 265 901 974 1240 1248
Size: 265 636 73 266 8
Fragments arranged by size:
636 266 265 73 8
BamHI G'GATC_C
Cuts at: 0 191 1202 1248
Size: 191 1011 46
Fragments arranged by size:
1011 191 46
BanI G'GyrC_C
Cuts at: 0 76 1248
Size: 76 1172
BanII G_rGCy'C
Cuts at: 0 628 647 981 1040 1248
Size: 628 19 334 59 208
Fragments arranged by size:
628 334 208 59 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 133 1248
Size: 133 1115
BbvCI CC'TCA_GC
Cuts at: 0 218 1248
Size: 218 1030
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 556 590 1248
Size: 556 34 658
Fragments arranged by size:
658 556 34
BclI T'GATC_A
Cuts at: 0 335 1248
Size: 335 913
BmrI ACTGGGnnnn_n'
Cuts at: 0 1190 1248
Size: 1190 58
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 607 1248
Size: 136 471 641
Fragments arranged by size:
641 471 136
Bpu10I CC'TnA_GC
Cuts at: 0 218 1248
Size: 218 1030
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 1248
Size: 289 959
BsaI GGTCTCn'nnnn_
Cuts at: 0 1200 1248
Size: 1200 48
BsaAI yAC'GTr
Cuts at: 0 10 603 1248
Size: 10 593 645
Fragments arranged by size:
645 593 10
BsaHI Gr'CG_yC
Cuts at: 0 383 473 1248
Size: 383 90 775
Fragments arranged by size:
775 383 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 610 640 1150 1180 1248
Size: 610 30 510 30 68
Fragments arranged by size:
610 510 68 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 955 1155 1158 1248
Size: 955 200 3 90
Fragments arranged by size:
955 200 90 3
BseYI C'CCAG_C
Cuts at: 0 140 252 411 1206 1248
Size: 140 112 159 795 42
Fragments arranged by size:
795 159 140 112 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 908 1123 1248
Size: 130 778 215 125
Fragments arranged by size:
778 215 130 125
BsiEI CG_ry'CG
Cuts at: 0 1113 1248
Size: 1113 135
BsiHKAI G_wGCw'C
Cuts at: 0 608 1248
Size: 608 640
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1167 1248
Size: 1167 81
Bsp1286I G_dGCh'C
Cuts at: 0 608 628 647 981 1040 1248
Size: 608 20 19 334 59 208
Fragments arranged by size:
608 334 208 59 20 19
BsrBI CCG'CTC
Cuts at: 0 50 767 921 1248
Size: 50 717 154 327
Fragments arranged by size:
717 327 154 50
BsrFI r'CCGG_y
Cuts at: 0 520 833 1016 1071 1248
Size: 520 313 183 55 177
Fragments arranged by size:
520 313 183 177 55
BstXI CCAn_nnnn'nTGG
Cuts at: 0 114 1075 1248
Size: 114 961 173
Fragments arranged by size:
961 173 114
BstYI r'GATC_y
Cuts at: 0 191 1202 1248
Size: 191 1011 46
Fragments arranged by size:
1011 191 46
BtgI C'CryG_G
Cuts at: 0 998 1040 1248
Size: 998 42 208
Fragments arranged by size:
998 208 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 69 1248
Size: 69 1179
BtsI GCAGTG_nn'
Cuts at: 0 1007 1032 1248
Size: 1007 25 216
Fragments arranged by size:
1007 216 25
EaeI y'GGCC_r
Cuts at: 0 323 455 1248
Size: 323 132 793
Fragments arranged by size:
793 323 132
EarI CTCTTCn'nnn_
Cuts at: 0 820 832 1248
Size: 820 12 416
Fragments arranged by size:
820 416 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 607 1248
Size: 136 471 641
Fragments arranged by size:
641 471 136
EcoO109I rG'GnC_Cy
Cuts at: 0 53 256 270 714 1248
Size: 53 203 14 444 534
Fragments arranged by size:
534 444 203 53 14
EcoRI G'AATT_C
Cuts at: 0 739 1248
Size: 739 509
EcoRV GAT'ATC
Cuts at: 0 66 1248
Size: 66 1182
HaeII r_GCGC'y
Cuts at: 0 666 1248
Size: 666 582
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 58 1248
Size: 26 32 1190
Fragments arranged by size:
1190 32 26
HincII GTy'rAC
Cuts at: 0 701 1110 1248
Size: 701 409 138
Fragments arranged by size:
701 409 138
MscI TGG'CCA
Cuts at: 0 457 1248
Size: 457 791
MslI CAynn'nnrTG
Cuts at: 0 1145 1248
Size: 1145 103
MspA1I CmG'CkG
Cuts at: 0 144 1248
Size: 144 1104
NaeI GCC'GGC
Cuts at: 0 1073 1248
Size: 1073 175
NgoMIV G'CCGG_C
Cuts at: 0 1071 1248
Size: 1071 177
NspI r_CATG'y
Cuts at: 0 876 1248
Size: 876 372
PflMI CCAn_nnn'nTGG
Cuts at: 0 206 1248
Size: 206 1042
PfoI T'CCnGG_A
Cuts at: 0 1198 1248
Size: 1198 50
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 145 177 259 291 1248
Size: 145 32 82 32 957
Fragments arranged by size:
957 145 82 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 53 270 714 1248
Size: 53 217 444 534
Fragments arranged by size:
534 444 217 53
PspXI vC'TCGA_Gb
Cuts at: 0 265 1248
Size: 265 983
PvuII CAG'CTG
Cuts at: 0 144 1248
Size: 144 1104
SalI G'TCGA_C
Cuts at: 0 1108 1248
Size: 1108 140
SapI GCTCTTCn'nnn_
Cuts at: 0 820 1248
Size: 820 428
SexAI A'CCwGG_T
Cuts at: 0 428 1248
Size: 428 820
SmlI C'TyrA_G
Cuts at: 0 265 304 1248
Size: 265 39 944
Fragments arranged by size:
944 265 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 64 496 1011 1248
Size: 64 432 515 237
Fragments arranged by size:
515 432 237 64
TatI w'GTAC_w
Cuts at: 0 611 1248
Size: 611 637
Tth111I GACn'n_nGTC
Cuts at: 0 856 1248
Size: 856 392
XhoI C'TCGA_G
Cuts at: 0 265 1248
Size: 265 983
ZraI GAC'GTC
Cuts at: 0 384 1248
Size: 384 864
Enzymes that do cut:
AatII AccI AflIII AleI AlfI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BcgI BclI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BstXI
BstYI BtgI BtgZI BtsI EaeI EarI Eco57MI EcoO109I
EcoRI EcoRV HaeII Hin4I HincII MscI MslI MspA1I
NaeI NgoMIV NspI PflMI PfoI PpiI PpuMI PspXI
PvuII SalI SapI SexAI SmlI TaqII TatI Tth111I
XhoI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BciVI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmtI BplI BsaBI BsaWI BsiWI BsmI BspEI
BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MmeI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PshAI PsiI PspOMI PsrI PstI
PvuI RsrII SacI SacII SanDI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TstI XbaI XcmI
XmaI XmnI