(Circular) (Six-base) MAPSORT of: pFN21AB3810.seq Check: 6721 from: 1 to: 6205 pFN21AB3810 6205 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4015 278 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3548 1937 Size: 1611 4594 Acc65I G'GTAC_C Cuts at: 3526 3526 Size: 6205 AclI AA'CG_TT Cuts at: 1563 4332 4705 1563 Size: 2769 373 3063 Fragments arranged by size: 3063 2769 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2046 2345 2747 3377 4268 5652 5920 1632 Size: 414 299 402 630 891 1384 268 1917 Fragments arranged by size: 1917 1384 891 630 414 402 299 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6008 AflIII A'CryG_T Cuts at: 1203 5104 6081 1203 Size: 3901 977 1327 Fragments arranged by size: 3901 1327 977 AgeI A'CCGG_T Cuts at: 5036 5036 Size: 6205 AhdI GACnn_n'nnGTC Cuts at: 4934 4934 Size: 6205 AleI CACnn'nnGTG Cuts at: 1166 2492 1166 Size: 1326 4879 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2510 1184 Size: 1326 4879 AlwNI CAG_nnn'CTG Cuts at: 3948 5520 3948 Size: 1572 4633 ApaLI G'TGCA_C Cuts at: 2621 2658 4262 5418 2621 Size: 37 1604 1156 3408 Fragments arranged by size: 3408 1604 1156 37 ApoI r'AATT_y Cuts at: 1493 3514 3740 3892 6001 1493 Size: 2021 226 152 2109 1697 Fragments arranged by size: 2109 2021 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4759 160 Size: 872 3727 1606 Fragments arranged by size: 3727 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 AvaI C'yCGr_G Cuts at: 1870 1943 2905 3530 1870 Size: 73 962 625 4545 Fragments arranged by size: 4545 962 625 73 BamHI G'GATC_C Cuts at: 3157 3397 3535 3902 3157 Size: 240 138 367 5460 Fragments arranged by size: 5460 367 240 138 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3526 4981 618 Size: 325 217 627 1739 1455 1842 Fragments arranged by size: 1842 1739 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2802 2906 3524 1391 Size: 1411 104 618 4072 Fragments arranged by size: 4072 1411 618 104 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5234 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2511 2545 4396 4430 1903 Size: 34 574 34 1851 34 3678 Fragments arranged by size: 3678 1851 574 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4097 5307 4097 Size: 1210 4995 BclI T'GATC_A Cuts at: 1548 2925 1548 Size: 1377 4828 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4816 136 Size: 107 122 71 631 605 3144 1525 Fragments arranged by size: 3144 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6200 6200 Size: 6205 BlpI GC'TnA_GC Cuts at: 3622 3622 Size: 6205 Bme1580I G_kGCm'C Cuts at: 1790 2625 2662 3108 4266 5422 1790 Size: 835 37 446 1158 1156 2573 Fragments arranged by size: 2573 1158 1156 835 446 37 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6205 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3936 4894 442 Size: 416 53 83 399 24 2519 958 1753 Fragments arranged by size: 2519 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6205 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2522 4865 1239 Size: 1283 2343 2579 Fragments arranged by size: 2579 2343 1283 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3111 4330 5195 5493 3111 Size: 1219 865 298 3823 Fragments arranged by size: 3823 1219 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3186 3429 4868 882 Size: 617 1687 243 1439 2219 Fragments arranged by size: 2219 1687 1439 617 243 BsaAI yAC'GTr Cuts at: 493 1204 3996 493 Size: 711 2792 2702 Fragments arranged by size: 2792 2702 711 BsaBI GATnn'nnATC Cuts at: 6199 6199 Size: 6205 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4012 4394 5029 275 Size: 53 83 186 936 2479 382 635 1451 Fragments arranged by size: 2479 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2310 2604 2806 4638 5036 5310 5457 5868 2310 Size: 294 202 1832 398 274 147 411 2647 Fragments arranged by size: 2647 1832 411 398 294 274 202 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2248 2278 1178 Size: 30 165 30 845 30 5105 Fragments arranged by size: 5105 845 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6205 BseYI C'CCAG_C Cuts at: 3366 5408 5904 3366 Size: 2042 496 3667 Fragments arranged by size: 3667 2042 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2194 3207 3413 1163 Size: 573 458 1013 206 3955 Fragments arranged by size: 3955 1013 573 458 206 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3655 4416 5444 1540 Size: 275 186 1654 761 1028 2301 Fragments arranged by size: 2301 1654 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2508 2625 2662 2802 2906 4266 4351 5422 2508 Size: 117 37 140 104 1360 85 1071 3291 Fragments arranged by size: 3291 1360 1071 140 117 104 85 37 BsmI GAATG_Cn' Cuts at: 3716 3809 3716 Size: 93 6112 BsmBI CGTCTCn'nnnn_ Cuts at: 2354 2354 Size: 6205 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2508 2625 2662 2802 2906 3108 3524 Size: 399 718 117 37 140 104 202 416 Cuts at: 3524 4266 4351 5422 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 718 416 399 202 140 117 104 85 37 BspEI T'CCGG_A Cuts at: 2604 5868 2604 Size: 3264 2941 BspHI T'CATG_A Cuts at: 4094 5824 4094 Size: 1730 4475 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2777 3560 844 Size: 1933 783 3489 Fragments arranged by size: 3489 1933 783 BsrBI CCG'CTC Cuts at: 1394 1823 4092 1394 Size: 429 2269 3507 Fragments arranged by size: 3507 2269 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2779 4700 4874 65 Size: 1183 1531 1921 174 1396 Fragments arranged by size: 1921 1531 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2580 4849 5036 1700 Size: 110 770 2269 187 2869 Fragments arranged by size: 2869 2269 770 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6205 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6205 BssSI C'ACGA_G Cuts at: 4265 5277 4265 Size: 1012 5193 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6205 BstBI TT'CG_AA Cuts at: 5009 5009 Size: 6205 BstEII G'GTnAC_C Cuts at: 1184 2447 1184 Size: 1263 4942 BstYI r'GATC_y Cuts at: 1972 2909 3157 3388 3397 3535 3902 4287 4304 Size: 937 248 231 9 138 367 385 17 Cuts at: 4304 5756 5842 5854 6200 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 937 385 367 346 248 231 138 86 17 12 9 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4714 Fragments arranged by size: 4714 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5466 Fragments arranged by size: 5466 683 56 BtsI GCAGTG_nn' Cuts at: 3721 4515 4535 3721 Size: 794 20 5391 Fragments arranged by size: 5391 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6205 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6170 DraI TTT'AAA Cuts at: 2413 3285 3509 2413 Size: 872 224 5109 Fragments arranged by size: 5109 872 224 DraIII CAC_nnn'GTG Cuts at: 2617 2617 Size: 6205 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5212 809 Size: 507 3896 1802 Fragments arranged by size: 3896 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3652 4541 Size: 54 1425 50 275 18 177 1645 889 Cuts at: 4541 6170 8 Size: 1629 43 Fragments arranged by size: 1645 1629 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3652 1537 Size: 275 1840 4090 Fragments arranged by size: 4090 1840 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2699 3115 4135 1353 Size: 1346 416 1020 3423 Fragments arranged by size: 3423 1346 1020 416 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4792 5166 5312 387 Size: 1064 138 3203 374 146 1280 Fragments arranged by size: 3203 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2800 2904 2800 Size: 104 6101 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2046 2345 2522 2747 3377 4268 4865 Size: 393 414 299 177 225 630 891 597 Cuts at: 4865 5652 5920 1239 Size: 787 268 1524 Fragments arranged by size: 1524 891 787 630 597 414 393 299 268 225 177 EcoO109I rG'GnC_Cy Cuts at: 2184 3171 2184 Size: 987 5218 EcoRI G'AATT_C Cuts at: 3514 3892 3514 Size: 378 5827 FspI TGC'GCA Cuts at: 4711 4711 Size: 6205 HaeII r_GCGC'y Cuts at: 1678 5352 1678 Size: 3674 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2698 2730 2822 2854 4846 Size: 32 135 32 1126 32 92 32 1992 Cuts at: 4846 4878 4920 4952 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1992 1126 135 92 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3549 3795 669 Size: 988 281 1611 246 3079 Fragments arranged by size: 3079 1611 988 281 246 HindIII A'AGCT_T Cuts at: 748 2402 3495 748 Size: 1654 1093 3458 Fragments arranged by size: 3458 1654 1093 HpaI GTT'AAC Cuts at: 3795 3795 Size: 6205 KpnI G_GTAC'C Cuts at: 3530 3530 Size: 6205 MfeI C'AATT_G Cuts at: 3804 3804 Size: 6205 MluI A'CGCG_T Cuts at: 6081 6081 Size: 6205 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3135 5319 5503 1305 Size: 1830 2184 184 2007 Fragments arranged by size: 2184 2007 1830 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 4206 Fragments arranged by size: 4206 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2492 2618 4163 4522 4681 518 Size: 648 1326 126 1545 359 159 2042 Fragments arranged by size: 2042 1545 1326 648 359 159 126 MspA1I CmG'CkG Cuts at: 1606 3621 4298 5446 5691 1606 Size: 2015 677 1148 245 2120 Fragments arranged by size: 2120 2015 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4714 Fragments arranged by size: 4714 939 552 NdeI CA'TA_TG Cuts at: 387 2697 3025 387 Size: 2310 328 3567 Fragments arranged by size: 3567 2310 328 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6205 NotI GC'GGCC_GC Cuts at: 3652 3652 Size: 6205 NspI r_CATG'y Cuts at: 1132 1229 2435 2955 3563 5108 1132 Size: 97 1206 520 608 1545 2229 Fragments arranged by size: 2229 1545 1206 608 520 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6205 PciI A'CATG_T Cuts at: 5104 5104 Size: 6205 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6205 PmeI GTTT'AAAC Cuts at: 3509 3509 Size: 6205 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4274 4306 5787 5819 4274 Size: 32 1481 32 4660 Fragments arranged by size: 4660 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3171 3171 Size: 6205 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6205 PsiI TTA'TAA Cuts at: 3775 3775 Size: 6205 PstI C_TGCA'G Cuts at: 830 3557 830 Size: 2727 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6205 SacI G_AGCT'C Cuts at: 2802 2906 2802 Size: 104 6101 SalI G'TCGA_C Cuts at: 1936 3547 1936 Size: 1611 4594 SanDI GG'GwC_CC Cuts at: 3171 3171 Size: 6205 SbfI CC_TGCA'GG Cuts at: 3557 3557 Size: 6205 ScaI AGT'ACT Cuts at: 4453 6165 4453 Size: 1712 4493 SfcI C'TryA_G Cuts at: 826 1045 3411 3553 4688 5369 5560 826 Size: 219 2366 142 1135 681 191 1471 Fragments arranged by size: 2366 1471 1135 681 219 191 142 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6205 SmaI CCC'GGG Cuts at: 1872 3532 1872 Size: 1660 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3126 4309 5210 5472 820 Size: 197 926 1183 1183 901 262 1553 Fragments arranged by size: 1553 1183 1183 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3996 493 Size: 3503 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6205 SphI G_CATG'C Cuts at: 1132 2435 3563 1132 Size: 1303 1128 3774 Fragments arranged by size: 3774 1303 1128 SspI AAT'ATT Cuts at: 5 52 4129 5 Size: 47 4077 2081 Fragments arranged by size: 4077 2081 47 StuI AGG'CCT Cuts at: 1517 2129 2219 1517 Size: 612 90 5503 Fragments arranged by size: 5503 612 90 StyI C'CwwG_G Cuts at: 513 1065 2004 2214 3322 3644 513 Size: 552 939 210 1108 322 3074 Fragments arranged by size: 3074 1108 939 552 322 210 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4232 4249 4402 4587 5032 325 Size: 1255 2652 17 153 185 445 1498 Fragments arranged by size: 2652 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2232 4451 6163 Size: 274 80 33 51 1443 255 2219 1712 Cuts at: 6163 96 Size: 138 Fragments arranged by size: 2219 1712 1443 274 255 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2002 2034 2962 2994 6165 6197 2002 Size: 32 928 32 3171 32 2010 Fragments arranged by size: 3171 2010 928 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6205 XbaI T'CTAG_A Cuts at: 3541 3541 Size: 6205 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6205 XmaI C'CCGG_G Cuts at: 1870 3530 1870 Size: 1660 4545 XmnI GAAnn'nnTTC Cuts at: 4334 4334 Size: 6205 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4013 276 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI