Restriction map for
pFN21AB3796
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI AspEI
AspI AvrII BbeI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BsrBI BsrGI Bst1107I BstAPI
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
EcoT22I FseI HaeII Hin1I HpaI
KasI KspI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AlwNI 945 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 168 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 83 CCCGAG C^[CT]CG[GA]G
BamHI 158 GGATCC G^GATCC
BbvCI 2281 CCTCAGC CCTCAGC(-5/-2)
BclI 236 TGATCA T^GATCA
BfaI 2339 CTAG C^TAG
BglI 1891 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 1159 GAGGAG GAGGAG(10/8)
BsoBI 83 CCCGAG C^[CT]CG[GA]G
BspLU11I 360 ACATGT A^CATGT
BssHII 7 GCGCGC G^CGCGC
BstEII 1995 GGTGACC G^GT[ACGT]ACC
BstPI 1995 GGTGACC G^GT[ACGT]ACC
EcoNI 2468 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1995 GGTGACC G^GT[ACGT]ACC
EcoRI 1988 GAATTC G^AATTC
FbaI 236 TGATCA T^GATCA
HindIII 730 AAGCTT A^AGCTT
MaeI 2339 CTAG C^TAG
NaeI 1622 GCCGGC GCC^GGC
NgoMIV 1622 GCCGGC G^CCGGC
PvuII 1273 CAGCTG CAG^CTG
SbfI 1282 CCTGCAGG CCTGCA^GG
SexAI 1467 ACCAGGT A^CC[AT]GGT
Sse8387I 1282 CCTGCAGG CCTGCA^GG
StuI 2172 AGGCCT AGG^CCT
XmnI 168 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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Acc65I 1007 GGTACC G^GTACC
1636 GGTACC
AcsI 706 GAATTT [GA]^AATT[CT]
1988 GAATTC
Alw44I 825 GTGCAC G^TGCAC
2333 GTGCAC
ApaLI 825 GTGCAC G^TGCAC
2333 GTGCAC
ApoI 706 GAATTT [GA]^AATT[CT]
1988 GAATTC
Asp718I 1007 GGTACC G^GTACC
1636 GGTACC
AviII 759 TGCGCA TGC^GCA
1243 TGCGCA
BalI 2441 TGGCCA TGG^CCA
2471 TGGCCA
BanII 2324 GGGCTC G[GA]GC[CT]^C
2500 GGGCTC
BsrFI 55 GCCGGT [GA]^CCGG[CT]
1622 GCCGGC
BssSI 1438 CACGAG CACGAG(-5/-1)
1825 CACGAG
Cfr10I 55 GCCGGT [GA]^CCGG[CT]
1622 GCCGGC
FspI 759 TGCGCA TGC^GCA
1243 TGCGCA
HincII 2245 GTTGAC GT[CT]^[GA]AC
2292 GTCAAC
HindII 2245 GTTGAC GT[CT]^[GA]AC
2292 GTCAAC
KpnI 1007 GGTACC GGTAC^C
1636 GGTACC
MluNI 2441 TGGCCA TGG^CCA
2471 TGGCCA
MscI 2441 TGGCCA TGG^CCA
2471 TGGCCA
NcoI 354 CCATGG C^CATGG
1019 CCATGG
PflMI 1627 CCAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2122 CCACCCCGTGG
SapI 110 GAAGAGC GCTCTTC(1/4)
168 GAAGAGC
SspI 205 AATATT AAT^ATT
1804 AATATT
Van91I 1627 CCAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2122 CCACCCCGTGG
XcmI 794 CCACCATCGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1982 CCATAGGAATTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AspHI AvaII BanI BbrPI
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PmaCI PmlI PpuMI PstI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII