(Circular) (Six-base) MAPSORT of: pFN21AB3796.seq Check: 4877 from: 1 to: 7285 pFN21AB3796 7285 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3512 3512 Size: 7285 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5095 278 Size: 53 83 186 936 3559 2468 Fragments arranged by size: 3559 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4628 1937 Size: 2691 4594 Acc65I G'GTAC_C Cuts at: 3012 3641 4606 3012 Size: 629 965 5691 Fragments arranged by size: 5691 965 629 AclI AA'CG_TT Cuts at: 1563 5412 5785 1563 Size: 3849 373 3063 Fragments arranged by size: 3849 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3251 3302 5348 6732 7000 1632 Size: 1619 51 2046 1384 268 1917 Fragments arranged by size: 2046 1917 1619 1384 268 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7088 AflIII A'CryG_T Cuts at: 1203 2365 2405 3372 3714 6184 7161 1203 Size: 1162 40 967 342 2470 977 1327 Fragments arranged by size: 2470 1327 1162 977 967 342 40 AgeI A'CCGG_T Cuts at: 6116 6116 Size: 7285 AhdI GACnn_n'nnGTC Cuts at: 6014 6014 Size: 7285 AleI CACnn'nnGTG Cuts at: 1166 3834 1166 Size: 2668 4617 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3862 1184 Size: 2678 4607 AlwNI CAG_nnn'CTG Cuts at: 2955 5028 6600 2955 Size: 2073 1572 3640 Fragments arranged by size: 3640 2073 1572 ApaLI G'TGCA_C Cuts at: 2830 4338 5342 6498 2830 Size: 1508 1004 1156 3617 Fragments arranged by size: 3617 1508 1156 1004 ApoI r'AATT_y Cuts at: 1493 2711 3993 4594 4820 4972 7081 1493 Size: 1218 1282 601 226 152 2109 1697 Fragments arranged by size: 2109 1697 1282 1218 601 226 152 AseI AT'TA_AT Cuts at: 160 1032 5839 160 Size: 872 4807 1606 Fragments arranged by size: 4807 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7285 AvaI C'yCGr_G Cuts at: 1870 1943 2088 4610 1870 Size: 73 145 2522 4545 Fragments arranged by size: 4545 2522 145 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2304 2337 2304 Size: 33 7252 BamHI G'GATC_C Cuts at: 2163 4615 4982 2163 Size: 2452 367 4466 Fragments arranged by size: 4466 2452 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3012 3620 3641 3904 4516 Size: 325 217 627 1225 608 21 263 612 Cuts at: 4516 4606 6061 618 Size: 90 1455 1842 Fragments arranged by size: 1842 1455 1225 627 612 608 325 263 217 90 21 BanII G_rGCy'C Cuts at: 1391 4333 4509 4604 1391 Size: 2942 176 95 4072 Fragments arranged by size: 4072 2942 176 95 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3276 4007 4058 4091 928 Size: 971 1377 731 51 33 4122 Fragments arranged by size: 4122 1377 971 731 51 33 BbvCI CC'TCA_GC Cuts at: 4287 4287 Size: 7285 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5476 5510 1903 Size: 34 3539 34 3678 Fragments arranged by size: 3678 3539 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5177 6387 5177 Size: 1210 6075 BclI T'GATC_A Cuts at: 1548 2241 1548 Size: 693 6592 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3902 5896 136 Size: 107 122 71 631 605 2230 1994 1525 Fragments arranged by size: 2230 1994 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7280 7280 Size: 7285 BlpI GC'TnA_GC Cuts at: 4702 4702 Size: 7285 Bme1580I G_kGCm'C Cuts at: 1790 2834 3393 3907 4342 4557 5346 6502 1790 Size: 1044 559 514 435 215 789 1156 2573 Fragments arranged by size: 2573 1156 1044 789 559 514 435 215 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7285 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2162 3910 4288 Size: 416 53 83 399 24 745 1748 378 Cuts at: 4288 4512 5016 5974 442 Size: 224 504 958 1753 Fragments arranged by size: 1753 1748 958 745 504 416 399 378 224 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7285 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2743 3089 3295 5945 1239 Size: 1504 346 206 2650 2579 Fragments arranged by size: 2650 2579 1504 346 206 Bpu10I CC'TnA_GC Cuts at: 4287 4287 Size: 7285 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5410 6275 6573 5410 Size: 865 298 6122 Fragments arranged by size: 6122 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2273 2420 3053 5948 882 Size: 617 774 147 633 2895 2219 Fragments arranged by size: 2895 2219 774 633 617 147 BsaAI yAC'GTr Cuts at: 493 1204 2406 3715 4337 5076 493 Size: 711 1202 1309 622 739 2702 Fragments arranged by size: 2702 1309 1202 739 711 622 BsaBI GATnn'nnATC Cuts at: 7279 7279 Size: 7285 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5092 5474 6109 275 Size: 53 83 186 936 3559 382 635 1451 Fragments arranged by size: 3559 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5718 6116 6390 6537 6948 5718 Size: 398 274 147 411 6055 Fragments arranged by size: 6055 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7060 Fragments arranged by size: 7060 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3179 1186 Size: 1993 5292 BseYI C'CCAG_C Cuts at: 2497 2911 3364 6488 6984 2497 Size: 414 453 3124 496 2798 Fragments arranged by size: 3124 2798 496 453 414 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2907 3322 3325 3365 3792 4362 1163 Size: 573 1171 415 3 40 427 570 4086 Fragments arranged by size: 4086 1171 573 570 427 415 40 3 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4735 5496 6524 1540 Size: 275 186 2734 761 1028 2301 Fragments arranged by size: 2734 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2068 2834 4342 5346 5431 6502 2068 Size: 766 1508 1004 85 1071 2851 Fragments arranged by size: 2851 1508 1071 1004 766 85 BsmI GAATG_Cn' Cuts at: 4796 4889 4796 Size: 93 7192 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2068 2834 3393 3907 4333 4342 4509 Size: 399 278 766 559 514 426 9 167 Cuts at: 4509 4557 4604 5346 5431 6502 1391 Size: 48 47 742 85 1071 2174 Fragments arranged by size: 2174 1071 766 742 559 514 426 399 278 167 85 48 47 9 BspEI T'CCGG_A Cuts at: 6948 6948 Size: 7285 BspHI T'CATG_A Cuts at: 2820 2935 2994 5174 6904 2820 Size: 115 59 2180 1730 3201 Fragments arranged by size: 3201 2180 1730 115 59 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3108 3387 3493 3512 4537 4640 844 Size: 2264 279 106 19 1025 103 3489 Fragments arranged by size: 3489 2264 1025 279 106 103 19 BsrBI CCG'CTC Cuts at: 1394 1823 5172 1394 Size: 429 3349 3507 Fragments arranged by size: 3507 3349 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2271 3217 3846 5780 5954 65 Size: 1183 1023 946 629 1934 174 1396 Fragments arranged by size: 1934 1396 1183 1023 946 629 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2060 3627 5929 6116 1700 Size: 110 250 1567 2302 187 2869 Fragments arranged by size: 2869 2302 1567 250 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7285 BssHII G'CGCG_C Cuts at: 1925 2012 1925 Size: 87 7198 BssSI C'ACGA_G Cuts at: 3443 3830 5345 6357 3443 Size: 387 1515 1012 4371 Fragments arranged by size: 4371 1515 1012 387 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7285 BstBI TT'CG_AA Cuts at: 6089 6089 Size: 7285 BstEII G'GTnAC_C Cuts at: 1184 4000 1184 Size: 2816 4469 BstXI CCAn_nnnn'nTGG Cuts at: 2809 3137 4494 2809 Size: 328 1357 5600 Fragments arranged by size: 5600 1357 328 BstYI r'GATC_y Cuts at: 1972 2163 3495 4438 4559 4615 4982 5367 5384 Size: 191 1332 943 121 56 367 385 17 Cuts at: 5384 6836 6922 6934 7280 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1332 943 385 367 346 191 121 86 56 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2359 3024 3850 4132 513 Size: 552 939 355 665 826 282 3666 Fragments arranged by size: 3666 939 826 665 552 355 282 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3741 487 Size: 683 56 2515 4031 Fragments arranged by size: 4031 2515 683 56 BtsI GCAGTG_nn' Cuts at: 3358 3458 3851 3886 4244 4801 5595 5615 3358 Size: 100 393 35 358 557 794 20 5028 Fragments arranged by size: 5028 794 557 393 358 100 35 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7285 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7250 DraI TTT'AAA Cuts at: 4589 4589 Size: 7285 DraIII CAC_nnn'GTG Cuts at: 3768 4133 4552 3768 Size: 365 419 6501 Fragments arranged by size: 6501 419 365 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6292 809 Size: 507 4976 1802 Fragments arranged by size: 4976 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 3629 4124 Size: 54 1425 50 275 18 228 1571 495 Cuts at: 4124 4162 4446 4476 4732 5621 7250 8 Size: 38 284 30 256 889 1629 43 Fragments arranged by size: 1629 1571 1425 889 495 284 275 256 228 54 50 43 38 30 18 EagI C'GGCC_G Cuts at: 1537 1812 4732 1537 Size: 275 2920 4090 Fragments arranged by size: 4090 2920 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2110 2168 2498 3551 5215 1353 Size: 757 58 330 1053 1664 3423 Fragments arranged by size: 3423 1664 1053 757 330 58 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4499 5872 6246 6392 387 Size: 1064 138 2910 1373 374 146 1280 Fragments arranged by size: 2910 1373 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2743 3089 3251 3295 3302 5348 5945 Size: 393 1111 346 162 44 7 2046 597 Cuts at: 5945 6732 7000 1239 Size: 787 268 1524 Fragments arranged by size: 2046 1524 1111 787 597 393 346 268 162 44 7 EcoNI CCTnn'n_nnAGG Cuts at: 4477 4477 Size: 7285 EcoO109I rG'GnC_Cy Cuts at: 2155 2689 3708 4398 4482 2155 Size: 534 1019 690 84 4958 Fragments arranged by size: 4958 1019 690 534 84 EcoRI G'AATT_C Cuts at: 3993 4594 4972 3993 Size: 601 378 6306 Fragments arranged by size: 6306 601 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3580 3946 3580 Size: 366 6919 FspI TGC'GCA Cuts at: 2766 3250 5791 2766 Size: 484 2541 4260 Fragments arranged by size: 4260 2541 484 FspAI rTGC'GCAy Cuts at: 3250 3250 Size: 7285 HaeII r_GCGC'y Cuts at: 1678 6432 1678 Size: 4754 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2233 2265 2475 2507 2660 Size: 32 135 32 661 32 210 32 153 Cuts at: 2660 2692 2976 3008 3377 3409 5926 5958 6000 Size: 32 284 32 369 32 2517 32 42 Cuts at: 6000 6032 1373 Size: 32 2626 Fragments arranged by size: 2626 2517 661 369 284 210 153 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4252 4299 4629 4875 669 Size: 988 281 2314 47 330 246 3079 Fragments arranged by size: 3079 2314 988 330 281 246 47 HindIII A'AGCT_T Cuts at: 748 2735 748 Size: 1987 5298 HpaI GTT'AAC Cuts at: 4875 4875 Size: 7285 KpnI G_GTAC'C Cuts at: 3016 3645 4610 3016 Size: 629 965 5691 Fragments arranged by size: 5691 965 629 MfeI C'AATT_G Cuts at: 4884 4884 Size: 7285 MluI A'CGCG_T Cuts at: 7161 7161 Size: 7285 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4165 6399 6583 1305 Size: 2860 2234 184 2007 Fragments arranged by size: 2860 2234 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4448 4478 10 Size: 54 1425 343 2616 30 2817 Fragments arranged by size: 2817 2616 1425 343 54 30 MslI CAynn'nnrTG Cuts at: 518 1166 2364 2760 2807 2825 3834 5243 5602 Size: 648 1198 396 47 18 1009 1409 359 Cuts at: 5602 5761 518 Size: 159 2042 Fragments arranged by size: 2042 1409 1198 1009 648 396 359 159 47 18 MspA1I CmG'CkG Cuts at: 1606 2020 3280 3951 4701 5378 6526 6771 1606 Size: 414 1260 671 750 677 1148 245 2120 Fragments arranged by size: 2120 1260 1148 750 677 671 414 245 NaeI GCC'GGC Cuts at: 3629 3629 Size: 7285 NcoI C'CATG_G Cuts at: 513 1065 2004 2359 3024 513 Size: 552 939 355 665 4774 Fragments arranged by size: 4774 939 665 552 355 NdeI CA'TA_TG Cuts at: 387 387 Size: 7285 NgoMIV G'CCGG_C Cuts at: 3627 3627 Size: 7285 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7285 NotI GC'GGCC_GC Cuts at: 4732 4732 Size: 7285 NspI r_CATG'y Cuts at: 1132 1229 2369 2830 3736 4643 6188 1132 Size: 97 1140 461 906 907 1545 2229 Fragments arranged by size: 2229 1545 1140 907 906 461 97 PasI CC'CwG_GG Cuts at: 1348 3491 3743 4393 1348 Size: 2143 252 650 4240 Fragments arranged by size: 4240 2143 650 252 PciI A'CATG_T Cuts at: 2365 6184 2365 Size: 3819 3466 PflMI CCAn_nnn'nTGG Cuts at: 1108 3638 4133 1108 Size: 2530 495 4260 Fragments arranged by size: 4260 2530 495 PmeI GTTT'AAAC Cuts at: 4589 4589 Size: 7285 PmlI CAC'GTG Cuts at: 2406 3715 4337 2406 Size: 1309 622 5354 Fragments arranged by size: 5354 1309 622 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5354 5386 6867 6899 5354 Size: 32 1481 32 5740 Fragments arranged by size: 5740 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2155 2689 3708 4398 4482 2155 Size: 534 1019 690 84 4958 Fragments arranged by size: 4958 1019 690 534 84 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7285 PsiI TTA'TAA Cuts at: 4855 4855 Size: 7285 PstI C_TGCA'G Cuts at: 830 3292 3523 3681 4239 4438 4637 830 Size: 2462 231 158 558 199 199 3478 Fragments arranged by size: 3478 2462 558 231 199 199 158 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7285 PvuII CAG'CTG Cuts at: 3280 3280 Size: 7285 SalI G'TCGA_C Cuts at: 1936 4627 1936 Size: 2691 4594 SanDI GG'GwC_CC Cuts at: 3708 3708 Size: 7285 SapI GCTCTTCn'nnn_ Cuts at: 2110 2168 2110 Size: 58 7227 SbfI CC_TGCA'GG Cuts at: 3292 4637 3292 Size: 1345 5940 ScaI AGT'ACT Cuts at: 5533 7245 5533 Size: 1712 5573 SexAI A'CCwGG_T Cuts at: 3472 3472 Size: 7285 SfcI C'TryA_G Cuts at: 826 1045 2956 3288 3519 3677 4235 4434 4633 Size: 219 1911 332 231 158 558 199 199 Cuts at: 4633 5768 6449 6640 826 Size: 1135 681 191 1471 Fragments arranged by size: 1911 1471 1135 681 558 332 231 219 199 199 191 158 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7285 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7285 SmaI CCC'GGG Cuts at: 1872 4612 1872 Size: 2740 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5389 6290 6552 820 Size: 197 926 3446 901 262 1553 Fragments arranged by size: 3446 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5076 493 Size: 4583 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7285 SphI G_CATG'C Cuts at: 1132 4643 1132 Size: 3511 3774 SspI AAT'ATT Cuts at: 5 52 2212 3811 5209 5 Size: 47 2160 1599 1398 2081 Fragments arranged by size: 2160 2081 1599 1398 47 StuI AGG'CCT Cuts at: 1517 4179 1517 Size: 2662 4623 StyI C'CwwG_G Cuts at: 513 1065 2004 2110 2359 2791 2881 3024 3976 Size: 552 939 106 249 432 90 143 952 Cuts at: 3976 4168 4473 4724 513 Size: 192 305 251 3074 Fragments arranged by size: 3074 952 939 552 432 305 251 249 192 143 106 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4580 5312 5329 5482 5667 6112 325 Size: 1255 3000 732 17 153 185 445 1498 Fragments arranged by size: 3000 1498 1255 732 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3717 5531 7243 Size: 274 80 33 51 1443 1740 1814 1712 Cuts at: 7243 96 Size: 138 Fragments arranged by size: 1814 1740 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2415 2447 3069 3101 3230 3262 3984 4016 4332 Size: 32 622 32 129 32 722 32 316 Cuts at: 4332 4364 7245 7277 2415 Size: 32 2881 32 2423 Fragments arranged by size: 2881 2423 722 622 316 129 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7285 XbaI T'CTAG_A Cuts at: 4621 4621 Size: 7285 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2806 3994 2806 Size: 1188 6097 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7285 XmaI C'CCGG_G Cuts at: 1870 4610 1870 Size: 2740 4545 XmnI GAAnn'nnTTC Cuts at: 2177 5414 2177 Size: 3237 4048 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5093 276 Size: 53 83 186 936 3559 2468 Fragments arranged by size: 3559 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI KasI NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI