Restriction map for
pFN21AB3796
(Linear) (Six-base) MAPSORT of: KIBB3796.seq Check: 4814 from: 1 to: 2580
KIBB3796.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1507 2580
Size: 1507 1073
Acc65I G'GTAC_C
Cuts at: 0 1007 1636 2580
Size: 1007 629 944
Fragments arranged by size:
1007 944 629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1246 1297 2580
Size: 1246 51 1283
Fragments arranged by size:
1283 1246 51
AflIII A'CryG_T
Cuts at: 0 360 400 1367 1709 2580
Size: 360 40 967 342 871
Fragments arranged by size:
967 871 360 342 40
AleI CACnn'nnGTG
Cuts at: 0 1829 2580
Size: 1829 751
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1857 2580
Size: 1857 723
AlwNI CAG_nnn'CTG
Cuts at: 0 950 2580
Size: 950 1630
ApaLI G'TGCA_C
Cuts at: 0 825 2333 2580
Size: 825 1508 247
Fragments arranged by size:
1508 825 247
ApoI r'AATT_y
Cuts at: 0 706 1988 2580
Size: 706 1282 592
Fragments arranged by size:
1282 706 592
AvaI C'yCGr_G
Cuts at: 0 83 2580
Size: 83 2497
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 299 332 2580
Size: 299 33 2248
Fragments arranged by size:
2248 299 33
BamHI G'GATC_C
Cuts at: 0 158 2580
Size: 158 2422
BanI G'GyrC_C
Cuts at: 0 1007 1615 1636 1899 2511 2580
Size: 1007 608 21 263 612 69
Fragments arranged by size:
1007 612 608 263 69 21
BanII G_rGCy'C
Cuts at: 0 2328 2504 2580
Size: 2328 176 76
Fragments arranged by size:
2328 176 76
BbsI GAAGACnn'nnnn_
Cuts at: 0 1271 2002 2053 2086 2580
Size: 1271 731 51 33 494
Fragments arranged by size:
1271 731 494 51 33
BbvCI CC'TCA_GC
Cuts at: 0 2282 2580
Size: 2282 298
BclI T'GATC_A
Cuts at: 0 236 2580
Size: 236 2344
BglI GCCn_nnn'nGGC
Cuts at: 0 1897 2580
Size: 1897 683
Bme1580I G_kGCm'C
Cuts at: 0 829 1388 1902 2337 2552 2580
Size: 829 559 514 435 215 28
Fragments arranged by size:
829 559 514 435 215 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 157 1905 2283 2507 2580
Size: 157 1748 378 224 73
Fragments arranged by size:
1748 378 224 157 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 738 1084 1290 2580
Size: 738 346 206 1290
Fragments arranged by size:
1290 738 346 206
Bpu10I CC'TnA_GC
Cuts at: 0 2282 2580
Size: 2282 298
BsaI GGTCTCn'nnnn_
Cuts at: 0 268 415 1048 2580
Size: 268 147 633 1532
Fragments arranged by size:
1532 633 268 147
BsaAI yAC'GTr
Cuts at: 0 401 1710 2332 2580
Size: 401 1309 622 248
Fragments arranged by size:
1309 622 401 248
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1174 2580
Size: 1174 1406
BseYI C'CCAG_C
Cuts at: 0 492 906 1359 2580
Size: 492 414 453 1221
Fragments arranged by size:
1221 492 453 414
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 1317 1320 1360 1787 2357 2580
Size: 902 415 3 40 427 570 223
Fragments arranged by size:
902 570 427 415 223 40 3
BsiHKAI G_wGCw'C
Cuts at: 0 63 829 2337 2580
Size: 63 766 1508 243
Fragments arranged by size:
1508 766 243 63
Bsp1286I G_dGCh'C
Cuts at: 0 63 829 1388 1902 2328 2337 2504 2552
Size: 63 766 559 514 426 9 167 48
Cuts at: 2552 2580
Size: 28
Fragments arranged by size:
766 559 514 426 167 63 48 28
9
BspHI T'CATG_A
Cuts at: 0 815 930 989 2580
Size: 815 115 59 1591
Fragments arranged by size:
1591 815 115 59
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1103 1382 1488 1507 2532 2580
Size: 1103 279 106 19 1025 48
Fragments arranged by size:
1103 1025 279 106 48 19
BsrDI GCAATG_nn'
Cuts at: 0 266 1212 1841 2580
Size: 266 946 629 739
Fragments arranged by size:
946 739 629 266
BsrFI r'CCGG_y
Cuts at: 0 55 1622 2580
Size: 55 1567 958
Fragments arranged by size:
1567 958 55
BssHII G'CGCG_C
Cuts at: 0 7 2580
Size: 7 2573
BssSI C'ACGA_G
Cuts at: 0 1438 1825 2580
Size: 1438 387 755
Fragments arranged by size:
1438 755 387
BstEII G'GTnAC_C
Cuts at: 0 1995 2580
Size: 1995 585
BstXI CCAn_nnnn'nTGG
Cuts at: 0 804 1132 2489 2580
Size: 804 328 1357 91
Fragments arranged by size:
1357 804 328 91
BstYI r'GATC_y
Cuts at: 0 158 1490 2433 2554 2580
Size: 158 1332 943 121 26
Fragments arranged by size:
1332 943 158 121 26
BtgI C'CryG_G
Cuts at: 0 354 1019 1845 2127 2580
Size: 354 665 826 282 453
Fragments arranged by size:
826 665 453 354 282
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1736 2580
Size: 1736 844
BtsI GCAGTG_nn'
Cuts at: 0 1353 1453 1846 1881 2239 2580
Size: 1353 100 393 35 358 341
Fragments arranged by size:
1353 393 358 341 100 35
DraIII CAC_nnn'GTG
Cuts at: 0 1763 2128 2547 2580
Size: 1763 365 419 33
Fragments arranged by size:
1763 419 365 33
EaeI y'GGCC_r
Cuts at: 0 53 1624 2119 2157 2441 2471 2580
Size: 53 1571 495 38 284 30 109
Fragments arranged by size:
1571 495 284 109 53 38 30
EarI CTCTTCn'nnn_
Cuts at: 0 105 163 493 1546 2580
Size: 105 58 330 1053 1034
Fragments arranged by size:
1053 1034 330 105 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2494 2580
Size: 2494 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 738 1084 1246 1290 1297 2580
Size: 738 346 162 44 7 1283
Fragments arranged by size:
1283 738 346 162 44 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2472 2580
Size: 2472 108
EcoO109I rG'GnC_Cy
Cuts at: 0 150 684 1703 2393 2477 2580
Size: 150 534 1019 690 84 103
Fragments arranged by size:
1019 690 534 150 103 84
EcoRI G'AATT_C
Cuts at: 0 1988 2580
Size: 1988 592
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1575 1941 2580
Size: 1575 366 639
Fragments arranged by size:
1575 639 366
FspI TGC'GCA
Cuts at: 0 761 1245 2580
Size: 761 484 1335
Fragments arranged by size:
1335 761 484
FspAI rTGC'GCAy
Cuts at: 0 1245 2580
Size: 1245 1335
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 228 260 470 502 655 687 971 1003
Size: 228 32 210 32 153 32 284 32
Cuts at: 1003 1372 1404 2580
Size: 369 32 1176
Fragments arranged by size:
1176 369 284 228 210 153 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2247 2294 2580
Size: 2247 47 286
Fragments arranged by size:
2247 286 47
HindIII A'AGCT_T
Cuts at: 0 730 2580
Size: 730 1850
KpnI G_GTAC'C
Cuts at: 0 1011 1640 2580
Size: 1011 629 940
Fragments arranged by size:
1011 940 629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2160 2580
Size: 2160 420
MscI TGG'CCA
Cuts at: 0 2443 2473 2580
Size: 2443 30 107
Fragments arranged by size:
2443 107 30
MslI CAynn'nnrTG
Cuts at: 0 359 755 802 820 1829 2580
Size: 359 396 47 18 1009 751
Fragments arranged by size:
1009 751 396 359 47 18
MspA1I CmG'CkG
Cuts at: 0 15 1275 1946 2580
Size: 15 1260 671 634
Fragments arranged by size:
1260 671 634 15
NaeI GCC'GGC
Cuts at: 0 1624 2580
Size: 1624 956
NcoI C'CATG_G
Cuts at: 0 354 1019 2580
Size: 354 665 1561
Fragments arranged by size:
1561 665 354
NgoMIV G'CCGG_C
Cuts at: 0 1622 2580
Size: 1622 958
NspI r_CATG'y
Cuts at: 0 364 825 1731 2580
Size: 364 461 906 849
Fragments arranged by size:
906 849 461 364
PasI CC'CwG_GG
Cuts at: 0 1486 1738 2388 2580
Size: 1486 252 650 192
Fragments arranged by size:
1486 650 252 192
PciI A'CATG_T
Cuts at: 0 360 2580
Size: 360 2220
PflMI CCAn_nnn'nTGG
Cuts at: 0 1633 2128 2580
Size: 1633 495 452
Fragments arranged by size:
1633 495 452
PmlI CAC'GTG
Cuts at: 0 401 1710 2332 2580
Size: 401 1309 622 248
Fragments arranged by size:
1309 622 401 248
PpuMI rG'GwC_Cy
Cuts at: 0 150 684 1703 2393 2477 2580
Size: 150 534 1019 690 84 103
Fragments arranged by size:
1019 690 534 150 103 84
PstI C_TGCA'G
Cuts at: 0 1287 1518 1676 2234 2433 2580
Size: 1287 231 158 558 199 147
Fragments arranged by size:
1287 558 231 199 158 147
PvuII CAG'CTG
Cuts at: 0 1275 2580
Size: 1275 1305
SanDI GG'GwC_CC
Cuts at: 0 1703 2580
Size: 1703 877
SapI GCTCTTCn'nnn_
Cuts at: 0 105 163 2580
Size: 105 58 2417
Fragments arranged by size:
2417 105 58
SbfI CC_TGCA'GG
Cuts at: 0 1287 2580
Size: 1287 1293
SexAI A'CCwGG_T
Cuts at: 0 1467 2580
Size: 1467 1113
SfcI C'TryA_G
Cuts at: 0 951 1283 1514 1672 2230 2429 2580
Size: 951 332 231 158 558 199 151
Fragments arranged by size:
951 558 332 231 199 158 151
SspI AAT'ATT
Cuts at: 0 207 1806 2580
Size: 207 1599 774
Fragments arranged by size:
1599 774 207
StuI AGG'CCT
Cuts at: 0 2174 2580
Size: 2174 406
StyI C'CwwG_G
Cuts at: 0 105 354 786 876 1019 1971 2163 2468
Size: 105 249 432 90 143 952 192 305
Cuts at: 2468 2580
Size: 112
Fragments arranged by size:
952 432 305 249 192 143 112 105
90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2575 2580
Size: 2575 5
TatI w'GTAC_w
Cuts at: 0 1712 2580
Size: 1712 868
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 410 442 1064 1096 1225 1257 1979 2011
Size: 410 32 622 32 129 32 722 32
Cuts at: 2011 2327 2359 2580
Size: 316 32 221
Fragments arranged by size:
722 622 410 316 221 129 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 801 1989 2580
Size: 801 1188 591
Fragments arranged by size:
1188 801 591
XmnI GAAnn'nnTTC
Cuts at: 0 172 2580
Size: 172 2408
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AlwNI ApaLI
ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BclI BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI
BsrFI BssHII BssSI BstEII BstXI BstYI BtgI BtgZI
BtsI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI Hin4I HincII HindIII KpnI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PciI PflMI PmlI PpuMI PstI PvuII SanDI
SapI SbfI SexAI SfcI SspI StuI StyI TaqII
TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AloI
ApaI AscI AseI AsiSI AvrII BbeI BcgI BciVI
BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrGI BstAPI BstBI BstZ17I Bsu36I ClaI CspCI
DraI DrdI EagI EcoICRI EcoRV FseI HaeII HpaI
KasI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SwaI Tth111I XbaI XhoI XmaI ZraI