(Circular) (Six-base) MAPSORT of: pFN21AB3767.seq Check: 9765 from: 1 to: 7984 pFN21AB3767 7984 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5794 278 Size: 53 83 186 936 4258 2468 Fragments arranged by size: 4258 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2645 3936 5327 1937 Size: 708 1291 1391 4594 Fragments arranged by size: 4594 1391 1291 708 Acc65I G'GTAC_C Cuts at: 4599 5305 4599 Size: 706 7278 AclI AA'CG_TT Cuts at: 1563 6111 6484 1563 Size: 4548 373 3063 Fragments arranged by size: 4548 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2278 2544 3144 3156 3914 4226 5079 6047 Size: 646 266 600 12 758 312 853 968 Cuts at: 6047 7431 7699 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 968 853 758 646 600 312 268 266 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7787 AflIII A'CryG_T Cuts at: 1203 6883 7860 1203 Size: 5680 977 1327 Fragments arranged by size: 5680 1327 977 AgeI A'CCGG_T Cuts at: 6815 6815 Size: 7984 AhdI GACnn_n'nnGTC Cuts at: 6713 6713 Size: 7984 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7984 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7984 AlwNI CAG_nnn'CTG Cuts at: 2053 3712 4282 5727 7299 2053 Size: 1659 570 1445 1572 2738 Fragments arranged by size: 2738 1659 1572 1445 570 ApaLI G'TGCA_C Cuts at: 6041 7197 6041 Size: 1156 6828 ApoI r'AATT_y Cuts at: 1493 2287 3359 3889 4333 4479 4507 5293 5519 Size: 794 1072 530 444 146 28 786 226 Cuts at: 5519 5671 7780 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1072 794 786 530 444 226 152 146 28 AseI AT'TA_AT Cuts at: 160 1032 6538 160 Size: 872 5506 1606 Fragments arranged by size: 5506 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7984 AvaI C'yCGr_G Cuts at: 1870 1943 2827 5309 1870 Size: 73 884 2482 4545 Fragments arranged by size: 4545 2482 884 73 BamHI G'GATC_C Cuts at: 4352 5314 5681 4352 Size: 962 367 6655 Fragments arranged by size: 6655 962 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2569 4599 5305 6760 618 Size: 325 217 627 782 2030 706 1455 1842 Fragments arranged by size: 2030 1842 1455 782 706 627 325 217 BanII G_rGCy'C Cuts at: 1391 2819 2828 5303 1391 Size: 1428 9 2475 4072 Fragments arranged by size: 4072 2475 1428 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2715 2852 3920 4482 928 Size: 971 816 137 1068 562 4430 Fragments arranged by size: 4430 1068 971 816 562 137 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6175 6209 1903 Size: 34 4238 34 3678 Fragments arranged by size: 4238 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2170 5876 7086 2170 Size: 3706 1210 3068 Fragments arranged by size: 3706 3068 1210 BclI T'GATC_A Cuts at: 1548 4876 1548 Size: 3328 4656 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6595 136 Size: 107 122 71 631 605 4923 1525 Fragments arranged by size: 4923 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3916 5218 7979 3916 Size: 1302 2761 3921 Fragments arranged by size: 3921 2761 1302 BlpI GC'TnA_GC Cuts at: 2043 2818 3288 5401 2043 Size: 775 470 2113 4626 Fragments arranged by size: 4626 2113 775 470 Bme1580I G_kGCm'C Cuts at: 1790 6045 7201 1790 Size: 4255 1156 2573 Fragments arranged by size: 4255 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7984 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5715 6673 442 Size: 416 53 83 399 24 4298 958 1753 Fragments arranged by size: 4298 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7984 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2017 2343 4238 4601 6644 1239 Size: 778 326 1895 363 2043 2579 Fragments arranged by size: 2579 2043 1895 778 363 326 Bpu10I CC'TnA_GC Cuts at: 4988 4988 Size: 7984 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4725 4792 6109 6974 7272 4725 Size: 67 1317 865 298 5437 Fragments arranged by size: 5437 1317 865 298 67 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2117 6647 882 Size: 617 618 4530 2219 Fragments arranged by size: 4530 2219 618 617 BsaAI yAC'GTr Cuts at: 493 1204 5775 493 Size: 711 4571 2702 Fragments arranged by size: 4571 2702 711 BsaBI GATnn'nnATC Cuts at: 5148 7978 5148 Size: 2830 5154 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5791 6173 6808 275 Size: 53 83 186 936 4258 382 635 1451 Fragments arranged by size: 4258 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6417 6815 7089 7236 7647 6417 Size: 398 274 147 411 6754 Fragments arranged by size: 6754 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2716 2746 3007 3037 3380 Size: 30 165 30 1313 30 261 30 343 Cuts at: 3380 3410 1178 Size: 30 5752 Fragments arranged by size: 5752 1313 343 261 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2724 3824 1186 Size: 931 607 1100 5346 Fragments arranged by size: 5346 1100 931 607 BseYI C'CCAG_C Cuts at: 2025 4545 7187 7683 2025 Size: 2520 2642 496 2326 Fragments arranged by size: 2642 2520 2326 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3698 4470 1163 Size: 573 1962 772 4677 Fragments arranged by size: 4677 1962 772 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5434 6195 7223 1540 Size: 275 186 3433 761 1028 2301 Fragments arranged by size: 3433 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2819 2828 6045 6130 7201 2819 Size: 9 3217 85 1071 3602 Fragments arranged by size: 3602 3217 1071 85 9 BsmI GAATG_Cn' Cuts at: 5495 5588 5495 Size: 93 7891 BsmBI CGTCTCn'nnnn_ Cuts at: 4646 4646 Size: 7984 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2819 2828 5303 6045 6130 7201 1391 Size: 399 1029 9 2475 742 85 1071 2174 Fragments arranged by size: 2475 2174 1071 1029 742 399 85 9 BspEI T'CCGG_A Cuts at: 7647 7647 Size: 7984 BspHI T'CATG_A Cuts at: 2794 4873 5873 7603 2794 Size: 2079 1000 1730 3175 Fragments arranged by size: 3175 2079 1730 1000 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2492 3310 5339 844 Size: 1648 818 2029 3489 Fragments arranged by size: 3489 2029 1648 818 BsrBI CCG'CTC Cuts at: 1394 1823 5871 1394 Size: 429 4048 3507 Fragments arranged by size: 4048 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4175 4313 6479 6653 65 Size: 1183 2927 138 2166 174 1396 Fragments arranged by size: 2927 2166 1396 1183 174 138 BsrFI r'CCGG_y Cuts at: 1700 1810 6628 6815 1700 Size: 110 4818 187 2869 Fragments arranged by size: 4818 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2995 5241 96 Size: 2899 2246 2839 Fragments arranged by size: 2899 2839 2246 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7984 BssSI C'ACGA_G Cuts at: 6044 7056 6044 Size: 1012 6972 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7984 BstBI TT'CG_AA Cuts at: 6788 6788 Size: 7984 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7984 BstXI CCAn_nnnn'nTGG Cuts at: 4156 4156 Size: 7984 BstYI r'GATC_y Cuts at: 1972 3666 3916 4352 5218 5314 5681 6066 6083 Size: 1694 250 436 866 96 367 385 17 Cuts at: 6083 7535 7621 7633 7979 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1694 1452 866 436 385 367 346 250 96 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2691 4166 2691 Size: 1475 6509 BtgI C'CryG_G Cuts at: 513 1065 3894 4494 513 Size: 552 2829 600 4003 Fragments arranged by size: 4003 2829 600 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7245 Fragments arranged by size: 7245 683 56 BtsI GCAGTG_nn' Cuts at: 3299 5500 6294 6314 3299 Size: 2201 794 20 4969 Fragments arranged by size: 4969 2201 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7984 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7949 DraI TTT'AAA Cuts at: 5288 5288 Size: 7984 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6991 809 Size: 507 5675 1802 Fragments arranged by size: 5675 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2422 3922 5431 Size: 54 1425 50 275 18 592 1500 1509 Cuts at: 5431 6320 7949 8 Size: 889 1629 43 Fragments arranged by size: 1629 1509 1500 1425 889 592 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5431 1537 Size: 275 3619 4090 Fragments arranged by size: 4090 3619 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2094 2330 2473 3801 3843 4644 5094 5190 Size: 741 236 143 1328 42 801 450 96 Cuts at: 5190 5914 1353 Size: 724 3423 Fragments arranged by size: 3423 1328 801 741 724 450 236 143 96 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6571 6945 7091 387 Size: 1064 138 4982 374 146 1280 Fragments arranged by size: 4982 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2817 2826 2817 Size: 9 7975 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2017 2278 2343 2544 3144 3156 3914 Size: 393 385 261 65 201 600 12 758 Cuts at: 3914 4226 4238 4601 5079 6047 6644 7431 7699 Size: 312 12 363 478 968 597 787 268 Cuts at: 7699 1239 Size: 1524 Fragments arranged by size: 1524 968 787 758 600 597 478 393 385 363 312 268 261 201 65 12 12 EcoNI CCTnn'n_nnAGG Cuts at: 2057 2057 Size: 7984 EcoO109I rG'GnC_Cy Cuts at: 2050 3832 4162 2050 Size: 1782 330 5872 Fragments arranged by size: 5872 1782 330 EcoRI G'AATT_C Cuts at: 4507 5293 5671 4507 Size: 786 378 6820 Fragments arranged by size: 6820 786 378 EcoRV GAT'ATC Cuts at: 3232 3232 Size: 7984 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3623 4106 4484 3623 Size: 483 378 7123 Fragments arranged by size: 7123 483 378 FspI TGC'GCA Cuts at: 6490 6490 Size: 7984 HaeII r_GCGC'y Cuts at: 1678 7131 1678 Size: 5453 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2837 2869 3032 3064 5205 Size: 32 135 32 1265 32 163 32 2141 Cuts at: 5205 5237 5262 5294 6625 6657 6699 6731 1373 Size: 32 25 32 1331 32 42 32 2626 Fragments arranged by size: 2626 2141 1331 1265 163 135 42 32 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 3214 5328 5574 669 Size: 988 281 1276 2114 246 3079 Fragments arranged by size: 3079 2114 1276 988 281 246 HindIII A'AGCT_T Cuts at: 748 3435 748 Size: 2687 5297 HpaI GTT'AAC Cuts at: 3214 5574 3214 Size: 2360 5624 KpnI G_GTAC'C Cuts at: 4603 5309 4603 Size: 706 7278 MfeI C'AATT_G Cuts at: 5583 5583 Size: 7984 MluI A'CGCG_T Cuts at: 7860 7860 Size: 7984 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7098 7282 1305 Size: 5793 184 2007 Fragments arranged by size: 5793 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2424 10 Size: 54 1425 343 592 5570 Fragments arranged by size: 5570 1425 592 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2799 4154 4366 5942 6301 6460 518 Size: 648 1633 1355 212 1576 359 159 2042 Fragments arranged by size: 2042 1633 1576 1355 648 359 212 159 MspA1I CmG'CkG Cuts at: 1606 3712 5400 6077 7225 7470 1606 Size: 2106 1688 677 1148 245 2120 Fragments arranged by size: 2120 2106 1688 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3894 4494 513 Size: 552 2829 600 4003 Fragments arranged by size: 4003 2829 600 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7984 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7984 NotI GC'GGCC_GC Cuts at: 5431 5431 Size: 7984 NspI r_CATG'y Cuts at: 1132 1229 5342 6887 1132 Size: 97 4113 1545 2229 Fragments arranged by size: 4113 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4348 1348 Size: 3000 4984 PciI A'CATG_T Cuts at: 6883 6883 Size: 7984 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 7082 PfoI T'CCnGG_A Cuts at: 2094 4254 2094 Size: 2160 5824 PmeI GTTT'AAAC Cuts at: 5288 5288 Size: 7984 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2941 2973 6053 6085 7566 7598 2941 Size: 32 3080 32 1481 32 3327 Fragments arranged by size: 3327 3080 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3832 4162 3832 Size: 330 7654 PshAI GACnn'nnGTC Cuts at: 1613 2270 1613 Size: 657 7327 PsiI TTA'TAA Cuts at: 5554 5554 Size: 7984 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4583 4615 4583 Size: 32 7952 PstI C_TGCA'G Cuts at: 830 3640 4099 5175 5336 830 Size: 2810 459 1076 161 3478 Fragments arranged by size: 3478 2810 1076 459 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7984 PvuII CAG'CTG Cuts at: 3712 3712 Size: 7984 SacI G_AGCT'C Cuts at: 2819 2828 2819 Size: 9 7975 SalI G'TCGA_C Cuts at: 1936 5326 1936 Size: 3390 4594 SbfI CC_TGCA'GG Cuts at: 5336 5336 Size: 7984 ScaI AGT'ACT Cuts at: 6232 7944 6232 Size: 1712 6272 SexAI A'CCwGG_T Cuts at: 4602 4602 Size: 7984 SfcI C'TryA_G Cuts at: 826 1045 2841 3636 4095 4455 4841 4970 5171 Size: 219 1796 795 459 360 386 129 201 Cuts at: 5171 5332 6467 7148 7339 826 Size: 161 1135 681 191 1471 Fragments arranged by size: 1796 1471 1135 795 681 459 386 360 219 201 191 161 129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7984 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7984 SmaI CCC'GGG Cuts at: 1872 5311 1872 Size: 3439 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2827 4740 4807 6088 6989 7251 Size: 197 926 884 1913 67 1281 901 262 Cuts at: 7251 820 Size: 1553 Fragments arranged by size: 1913 1553 1281 926 901 884 262 197 67 SnaBI TAC'GTA Cuts at: 493 5775 493 Size: 5282 2702 SpeI A'CTAG_T Cuts at: 152 5065 152 Size: 4913 3071 SphI G_CATG'C Cuts at: 1132 5342 1132 Size: 4210 3774 SspI AAT'ATT Cuts at: 5 52 3877 5908 5 Size: 47 3825 2031 2081 Fragments arranged by size: 3825 2081 2031 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7984 StyI C'CwwG_G Cuts at: 513 1065 3894 4494 5423 513 Size: 552 2829 600 929 3074 Fragments arranged by size: 3074 2829 929 600 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6011 6028 6181 6366 6811 325 Size: 1255 4431 17 153 185 445 1498 Fragments arranged by size: 4431 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2995 5241 6230 Size: 274 80 33 51 1443 1018 2246 989 Cuts at: 6230 7942 96 Size: 1712 138 Fragments arranged by size: 2246 1712 1443 1018 989 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3007 3039 3255 3287 7944 7976 3007 Size: 32 216 32 4657 32 3015 Fragments arranged by size: 4657 3015 216 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4363 5274 1316 Size: 3047 911 4026 Fragments arranged by size: 4026 3047 911 XbaI T'CTAG_A Cuts at: 3427 5032 5320 3427 Size: 1605 288 6091 Fragments arranged by size: 6091 1605 288 XhoI C'TCGA_G Cuts at: 1943 2827 1943 Size: 884 7100 XmaI C'CCGG_G Cuts at: 1870 5309 1870 Size: 3439 4545 XmnI GAAnn'nnTTC Cuts at: 2152 5208 6113 2152 Size: 3056 905 4023 Fragments arranged by size: 4023 3056 905 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5792 276 Size: 53 83 186 936 4258 2468 Fragments arranged by size: 4258 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI