(Circular) (Six-base) MAPSORT of: pFN21AB3736.seq Check: 8404 from: 1 to: 6058 pFN21AB3736 6058 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2956 3235 2956 Size: 279 5779 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3868 278 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2685 3401 1937 Size: 748 716 4594 Fragments arranged by size: 4594 748 716 Acc65I G'GTAC_C Cuts at: 3379 3379 Size: 6058 AclI AA'CG_TT Cuts at: 1563 4185 4558 1563 Size: 2622 373 3063 Fragments arranged by size: 3063 2622 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3143 4121 5505 5773 1632 Size: 1511 978 1384 268 1917 Fragments arranged by size: 1917 1511 1384 978 268 AfeI AGC'GCT Cuts at: 2423 2423 Size: 6058 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5861 AflIII A'CryG_T Cuts at: 1203 4957 5934 1203 Size: 3754 977 1327 Fragments arranged by size: 3754 1327 977 AgeI A'CCGG_T Cuts at: 4889 4889 Size: 6058 AhdI GACnn_n'nnGTC Cuts at: 3171 4787 3171 Size: 1616 4442 AleI CACnn'nnGTG Cuts at: 1166 2596 1166 Size: 1430 4628 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6058 AlwNI CAG_nnn'CTG Cuts at: 2893 3801 5373 2893 Size: 908 1572 3578 Fragments arranged by size: 3578 1572 908 ApaI G_GGCC'C Cuts at: 2818 2818 Size: 6058 ApaLI G'TGCA_C Cuts at: 4115 5271 4115 Size: 1156 4902 ApoI r'AATT_y Cuts at: 1493 2228 3367 3593 3745 5854 1493 Size: 735 1139 226 152 2109 1697 Fragments arranged by size: 2109 1697 1139 735 226 152 AseI AT'TA_AT Cuts at: 160 1032 4612 160 Size: 872 3580 1606 Fragments arranged by size: 3580 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 AvaI C'yCGr_G Cuts at: 1870 1943 3383 1870 Size: 73 1440 4545 Fragments arranged by size: 4545 1440 73 BamHI G'GATC_C Cuts at: 3388 3755 3388 Size: 367 5691 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3379 4834 618 Size: 325 217 627 1592 1455 1842 Fragments arranged by size: 1842 1592 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2569 2818 3060 3131 3377 1391 Size: 1178 249 242 71 246 4072 Fragments arranged by size: 4072 1178 249 246 242 71 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2227 2233 928 Size: 971 328 6 4753 Fragments arranged by size: 4753 971 328 6 BbvCI CC'TCA_GC Cuts at: 3107 3261 3107 Size: 154 5904 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4249 4283 1903 Size: 34 2312 34 3678 Fragments arranged by size: 3678 2312 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3950 5160 3950 Size: 1210 4848 BclI T'GATC_A Cuts at: 1548 1548 Size: 6058 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2714 3126 4669 Size: 107 122 71 631 605 1042 412 1543 Cuts at: 4669 136 Size: 1525 Fragments arranged by size: 1543 1525 1042 631 605 412 122 107 71 BglII A'GATC_T Cuts at: 6053 6053 Size: 6058 BlpI GC'TnA_GC Cuts at: 2946 3059 3475 2946 Size: 113 416 5529 Fragments arranged by size: 5529 416 113 Bme1580I G_kGCm'C Cuts at: 1790 2818 4119 5275 1790 Size: 1028 1301 1156 2573 Fragments arranged by size: 2573 1301 1156 1028 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6058 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2020 2565 2970 Size: 416 53 83 399 24 603 545 405 Cuts at: 2970 3789 4747 442 Size: 819 958 1753 Fragments arranged by size: 1753 958 819 603 545 416 405 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6058 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2079 3286 2079 Size: 1207 4851 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2287 2579 3078 3302 3371 4718 1239 Size: 1048 292 499 224 69 1347 2579 Fragments arranged by size: 2579 1347 1048 499 292 224 69 Bpu10I CC'TnA_GC Cuts at: 2809 3107 3261 2809 Size: 298 154 5606 Fragments arranged by size: 5606 298 154 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4183 5048 5346 4183 Size: 865 298 4895 Fragments arranged by size: 4895 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2554 3260 4721 882 Size: 617 1055 706 1461 2219 Fragments arranged by size: 2219 1461 1055 706 617 BsaAI yAC'GTr Cuts at: 493 1204 2434 2745 3849 493 Size: 711 1230 311 1104 2702 Fragments arranged by size: 2702 1230 1104 711 311 BsaBI GATnn'nnATC Cuts at: 6052 6052 Size: 6058 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2213 3865 4247 4882 Size: 53 83 186 936 680 1652 382 635 Cuts at: 4882 275 Size: 1451 Fragments arranged by size: 1652 1451 936 680 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2835 4491 4889 5163 5310 5721 2835 Size: 1656 398 274 147 411 3172 Fragments arranged by size: 3172 1656 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2593 2623 1178 Size: 30 165 30 1190 30 4613 Fragments arranged by size: 4613 1190 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2660 3113 3116 3152 3278 1186 Size: 1474 453 3 36 126 3966 Fragments arranged by size: 3966 1474 453 126 36 3 BseYI C'CCAG_C Cuts at: 2470 3206 5261 5757 2470 Size: 736 2055 496 2771 Fragments arranged by size: 2771 2055 736 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5485 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3508 4269 5297 1540 Size: 275 186 1507 761 1028 2301 Fragments arranged by size: 2301 1507 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2569 2947 3060 4119 4204 5275 2569 Size: 378 113 1059 85 1071 3352 Fragments arranged by size: 3352 1071 1059 378 113 85 BsmI GAATG_Cn' Cuts at: 3569 3662 3569 Size: 93 5965 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2569 2818 2947 3060 3131 3377 4119 Size: 399 779 249 129 113 71 246 742 Cuts at: 4119 4204 5275 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 779 742 399 249 246 129 113 85 71 BspEI T'CCGG_A Cuts at: 5721 5721 Size: 6058 BspHI T'CATG_A Cuts at: 3947 5677 3947 Size: 1730 4328 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2271 2956 3235 3413 844 Size: 1427 685 279 178 3489 Fragments arranged by size: 3489 1427 685 279 178 BsrBI CCG'CTC Cuts at: 1394 1823 2790 3945 1394 Size: 429 967 1155 3507 Fragments arranged by size: 3507 1155 967 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2188 4553 4727 65 Size: 1183 940 2365 174 1396 Fragments arranged by size: 2365 1396 1183 940 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3039 4702 4889 1700 Size: 110 1229 1663 187 2869 Fragments arranged by size: 2869 1663 1229 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6058 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6058 BssSI C'ACGA_G Cuts at: 4118 5130 4118 Size: 1012 5046 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6058 BstBI TT'CG_AA Cuts at: 3241 4862 3241 Size: 1621 4437 BstEII G'GTnAC_C Cuts at: 1184 2800 2980 1184 Size: 1616 180 4262 Fragments arranged by size: 4262 1616 180 BstXI CCAn_nnnn'nTGG Cuts at: 2416 2749 2416 Size: 333 5725 BstYI r'GATC_y Cuts at: 1972 3045 3388 3755 4140 4157 5609 5695 5707 Size: 1073 343 367 385 17 1452 86 12 Cuts at: 5707 6053 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1073 385 367 346 343 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2520 513 Size: 552 939 516 4051 Fragments arranged by size: 4051 939 552 516 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5319 Fragments arranged by size: 5319 683 56 BtsI GCAGTG_nn' Cuts at: 2868 3574 4368 4388 2868 Size: 706 794 20 4538 Fragments arranged by size: 4538 794 706 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6058 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6023 DraI TTT'AAA Cuts at: 3362 3362 Size: 6058 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5065 809 Size: 507 3749 1802 Fragments arranged by size: 3749 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2196 2292 2388 Size: 54 1425 50 275 18 366 96 96 Cuts at: 2388 2517 3505 4394 6023 8 Size: 129 988 889 1629 43 Fragments arranged by size: 1629 1425 988 889 366 275 129 96 96 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3505 1537 Size: 275 1693 4090 Fragments arranged by size: 4090 1693 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2047 2147 3988 1353 Size: 694 100 1841 3423 Fragments arranged by size: 3423 1841 694 100 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4645 5019 5165 387 Size: 1064 138 3056 374 146 1280 Fragments arranged by size: 3056 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2567 3058 2567 Size: 491 5567 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2287 2579 3078 3143 3302 3371 4121 Size: 393 655 292 499 65 159 69 750 Cuts at: 4121 4718 5505 5773 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 750 655 597 499 393 292 268 159 69 65 EcoO109I rG'GnC_Cy Cuts at: 2059 2075 2095 2814 2815 2059 Size: 16 20 719 1 5302 Fragments arranged by size: 5302 719 20 16 1 EcoRI G'AATT_C Cuts at: 3367 3745 3367 Size: 378 5680 FspI TGC'GCA Cuts at: 2462 4564 2462 Size: 2102 3956 FspAI rTGC'GCAy Cuts at: 2462 2462 Size: 6058 HaeII r_GCGC'y Cuts at: 1678 2425 5205 1678 Size: 747 2780 2531 Fragments arranged by size: 2780 2531 747 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3157 3189 4699 4731 4773 Size: 32 135 32 1585 32 1510 32 42 Cuts at: 4773 4805 1373 Size: 32 2626 Fragments arranged by size: 2626 1585 1510 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3235 3402 3648 669 Size: 988 281 1297 167 246 3079 Fragments arranged by size: 3079 1297 988 281 246 167 HindIII A'AGCT_T Cuts at: 748 748 Size: 6058 HpaI GTT'AAC Cuts at: 3648 3648 Size: 6058 KpnI G_GTAC'C Cuts at: 3383 3383 Size: 6058 MfeI C'AATT_G Cuts at: 3657 3657 Size: 6058 MluI A'CGCG_T Cuts at: 5934 5934 Size: 6058 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3036 5172 5356 1305 Size: 1731 2136 184 2007 Fragments arranged by size: 2136 2007 1731 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2294 2390 2519 10 Size: 54 1425 343 462 96 129 3549 Fragments arranged by size: 3549 1425 462 343 129 96 54 MslI CAynn'nnrTG Cuts at: 518 1166 2414 2596 2678 2747 2928 3308 4016 Size: 648 1248 182 82 69 181 380 708 Cuts at: 4016 4375 4534 518 Size: 359 159 2042 Fragments arranged by size: 2042 1248 708 648 380 359 182 181 159 82 69 MspA1I CmG'CkG Cuts at: 1606 2189 2737 3474 4151 5299 5544 1606 Size: 583 548 737 677 1148 245 2120 Fragments arranged by size: 2120 1148 737 677 583 548 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2520 513 Size: 552 939 516 4051 Fragments arranged by size: 4051 939 552 516 NdeI CA'TA_TG Cuts at: 387 387 Size: 6058 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6058 NotI GC'GGCC_GC Cuts at: 3505 3505 Size: 6058 NspI r_CATG'y Cuts at: 1132 1229 2698 3416 4961 1132 Size: 97 1469 718 1545 2229 Fragments arranged by size: 2229 1545 1469 718 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6058 PciI A'CATG_T Cuts at: 4957 4957 Size: 6058 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6058 PfoI T'CCnGG_A Cuts at: 2097 3279 2097 Size: 1182 4876 PmeI GTTT'AAAC Cuts at: 3362 3362 Size: 6058 PmlI CAC'GTG Cuts at: 2745 2745 Size: 6058 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4127 4159 5640 5672 4127 Size: 32 1481 32 4513 Fragments arranged by size: 4513 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2059 2075 2095 2059 Size: 16 20 6022 Fragments arranged by size: 6022 20 16 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6058 PsiI TTA'TAA Cuts at: 3628 3628 Size: 6058 PspOMI G'GGCC_C Cuts at: 2814 2814 Size: 6058 PstI C_TGCA'G Cuts at: 830 2693 3153 3410 830 Size: 1863 460 257 3478 Fragments arranged by size: 3478 1863 460 257 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6058 SacI G_AGCT'C Cuts at: 2569 3060 2569 Size: 491 5567 SalI G'TCGA_C Cuts at: 1936 3400 1936 Size: 1464 4594 SanDI GG'GwC_CC Cuts at: 2059 2095 2059 Size: 36 6022 SbfI CC_TGCA'GG Cuts at: 3410 3410 Size: 6058 ScaI AGT'ACT Cuts at: 4306 6018 4306 Size: 1712 4346 SfcI C'TryA_G Cuts at: 826 1045 2689 3149 3406 4541 5222 5413 826 Size: 219 1644 460 257 1135 681 191 1471 Fragments arranged by size: 1644 1471 1135 681 460 257 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6058 SmaI CCC'GGG Cuts at: 1872 3385 1872 Size: 1513 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4162 5063 5325 820 Size: 197 926 2219 901 262 1553 Fragments arranged by size: 2219 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2434 3849 493 Size: 1941 1415 2702 Fragments arranged by size: 2702 1941 1415 SpeI A'CTAG_T Cuts at: 152 152 Size: 6058 SphI G_CATG'C Cuts at: 1132 3416 1132 Size: 2284 3774 SspI AAT'ATT Cuts at: 5 52 3982 5 Size: 47 3930 2081 Fragments arranged by size: 3930 2081 47 StuI AGG'CCT Cuts at: 1517 2513 1517 Size: 996 5062 StyI C'CwwG_G Cuts at: 513 1065 2004 2514 2520 3497 513 Size: 552 939 510 6 977 3074 Fragments arranged by size: 3074 977 939 552 510 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2032 2486 2494 4085 4102 4255 4440 Size: 1255 452 454 8 1591 17 153 185 Cuts at: 4440 4885 325 Size: 445 1498 Fragments arranged by size: 1591 1498 1255 454 452 445 185 153 17 8 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4304 6016 96 Size: 274 80 33 51 1443 2327 1712 138 Fragments arranged by size: 2327 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2593 2625 6018 6050 2593 Size: 32 3393 32 2601 Fragments arranged by size: 3393 2601 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6058 XbaI T'CTAG_A Cuts at: 3394 3394 Size: 6058 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2330 2330 Size: 6058 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6058 XmaI C'CCGG_G Cuts at: 1870 3383 1870 Size: 1513 4545 XmnI GAAnn'nnTTC Cuts at: 4187 4187 Size: 6058 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3866 276 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI