Restriction map for
pFN21AB3736
(Linear) (Six-base) MAPSORT of: KIBB3736.seq Check: 4007 from: 1 to: 1353
KIBB3736.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 951 1230 1353
Size: 951 279 123
Fragments arranged by size:
951 279 123
AccI GT'mk_AC
Cuts at: 0 680 1353
Size: 680 673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1138 1353
Size: 1138 215
AfeI AGC'GCT
Cuts at: 0 418 1353
Size: 418 935
AhdI GACnn_n'nnGTC
Cuts at: 0 1166 1353
Size: 1166 187
AleI CACnn'nnGTG
Cuts at: 0 591 1353
Size: 591 762
AlwNI CAG_nnn'CTG
Cuts at: 0 888 1353
Size: 888 465
ApaI G_GGCC'C
Cuts at: 0 813 1353
Size: 813 540
ApoI r'AATT_y
Cuts at: 0 223 1353
Size: 223 1130
BanII G_rGCy'C
Cuts at: 0 564 813 1055 1126 1353
Size: 564 249 242 71 227
Fragments arranged by size:
564 249 242 227 71
BbsI GAAGACnn'nnnn_
Cuts at: 0 222 228 1353
Size: 222 6 1125
Fragments arranged by size:
1125 222 6
BbvCI CC'TCA_GC
Cuts at: 0 1102 1256 1353
Size: 1102 154 97
Fragments arranged by size:
1102 154 97
BglI GCCn_nnn'nGGC
Cuts at: 0 709 1121 1353
Size: 709 412 232
Fragments arranged by size:
709 412 232
BlpI GC'TnA_GC
Cuts at: 0 941 1054 1353
Size: 941 113 299
Fragments arranged by size:
941 299 113
Bme1580I G_kGCm'C
Cuts at: 0 813 1353
Size: 813 540
BmrI ACTGGGnnnn_n'
Cuts at: 0 15 560 965 1353
Size: 15 545 405 388
Fragments arranged by size:
545 405 388 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 74 1281 1353
Size: 74 1207 72
Fragments arranged by size:
1207 74 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 574 1073 1297 1353
Size: 282 292 499 224 56
Fragments arranged by size:
499 292 282 224 56
Bpu10I CC'TnA_GC
Cuts at: 0 804 1102 1256 1353
Size: 804 298 154 97
Fragments arranged by size:
804 298 154 97
BsaI GGTCTCn'nnnn_
Cuts at: 0 549 1255 1353
Size: 549 706 98
Fragments arranged by size:
706 549 98
BsaAI yAC'GTr
Cuts at: 0 429 740 1353
Size: 429 311 613
Fragments arranged by size:
613 429 311
BsaHI Gr'CG_yC
Cuts at: 0 208 1353
Size: 208 1145
BsaWI w'CCGG_w
Cuts at: 0 830 1353
Size: 830 523
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 588 618 1353
Size: 588 30 735
Fragments arranged by size:
735 588 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 655 1108 1111 1147 1273 1353
Size: 655 453 3 36 126 80
Fragments arranged by size:
655 453 126 80 36 3
BseYI C'CCAG_C
Cuts at: 0 465 1201 1353
Size: 465 736 152
Fragments arranged by size:
736 465 152
BsiHKAI G_wGCw'C
Cuts at: 0 564 942 1055 1353
Size: 564 378 113 298
Fragments arranged by size:
564 378 298 113
Bsp1286I G_dGCh'C
Cuts at: 0 564 813 942 1055 1126 1353
Size: 564 249 129 113 71 227
Fragments arranged by size:
564 249 227 129 113 71
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 266 951 1230 1353
Size: 266 685 279 123
Fragments arranged by size:
685 279 266 123
BsrBI CCG'CTC
Cuts at: 0 785 1353
Size: 785 568
BsrDI GCAATG_nn'
Cuts at: 0 183 1353
Size: 183 1170
BsrFI r'CCGG_y
Cuts at: 0 1034 1353
Size: 1034 319
BstBI TT'CG_AA
Cuts at: 0 1236 1353
Size: 1236 117
BstEII G'GTnAC_C
Cuts at: 0 795 975 1353
Size: 795 180 378
Fragments arranged by size:
795 378 180
BstXI CCAn_nnnn'nTGG
Cuts at: 0 411 744 1353
Size: 411 333 609
Fragments arranged by size:
609 411 333
BstYI r'GATC_y
Cuts at: 0 1040 1353
Size: 1040 313
BtgI C'CryG_G
Cuts at: 0 515 1353
Size: 515 838
BtsI GCAGTG_nn'
Cuts at: 0 863 1353
Size: 863 490
EaeI y'GGCC_r
Cuts at: 0 191 287 383 512 1353
Size: 191 96 96 129 841
Fragments arranged by size:
841 191 129 96 96
EarI CTCTTCn'nnn_
Cuts at: 0 42 142 1353
Size: 42 100 1211
Fragments arranged by size:
1211 100 42
EcoICRI GAG'CTC
Cuts at: 0 562 1053 1353
Size: 562 491 300
Fragments arranged by size:
562 491 300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 574 1073 1138 1297 1353
Size: 282 292 499 65 159 56
Fragments arranged by size:
499 292 282 159 65 56
EcoO109I rG'GnC_Cy
Cuts at: 0 54 70 90 809 810 1353
Size: 54 16 20 719 1 543
Fragments arranged by size:
719 543 54 20 16 1
FspI TGC'GCA
Cuts at: 0 457 1353
Size: 457 896
FspAI rTGC'GCAy
Cuts at: 0 457 1353
Size: 457 896
HaeII r_GCGC'y
Cuts at: 0 420 1353
Size: 420 933
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1152 1184 1353
Size: 1152 32 169
Fragments arranged by size:
1152 169 32
HincII GTy'rAC
Cuts at: 0 1230 1353
Size: 1230 123
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1031 1353
Size: 1031 322
MscI TGG'CCA
Cuts at: 0 289 385 514 1353
Size: 289 96 129 839
Fragments arranged by size:
839 289 129 96
MslI CAynn'nnrTG
Cuts at: 0 409 591 673 742 923 1303 1353
Size: 409 182 82 69 181 380 50
Fragments arranged by size:
409 380 182 181 82 69 50
MspA1I CmG'CkG
Cuts at: 0 184 732 1353
Size: 184 548 621
Fragments arranged by size:
621 548 184
NcoI C'CATG_G
Cuts at: 0 515 1353
Size: 515 838
NspI r_CATG'y
Cuts at: 0 693 1353
Size: 693 660
PfoI T'CCnGG_A
Cuts at: 0 92 1274 1353
Size: 92 1182 79
Fragments arranged by size:
1182 92 79
PmlI CAC'GTG
Cuts at: 0 740 1353
Size: 740 613
PpuMI rG'GwC_Cy
Cuts at: 0 54 70 90 1353
Size: 54 16 20 1263
Fragments arranged by size:
1263 54 20 16
PspOMI G'GGCC_C
Cuts at: 0 809 1353
Size: 809 544
PstI C_TGCA'G
Cuts at: 0 688 1148 1353
Size: 688 460 205
Fragments arranged by size:
688 460 205
SacI G_AGCT'C
Cuts at: 0 564 1055 1353
Size: 564 491 298
Fragments arranged by size:
564 491 298
SanDI GG'GwC_CC
Cuts at: 0 54 90 1353
Size: 54 36 1263
Fragments arranged by size:
1263 54 36
SfcI C'TryA_G
Cuts at: 0 684 1144 1353
Size: 684 460 209
Fragments arranged by size:
684 460 209
SnaBI TAC'GTA
Cuts at: 0 429 1353
Size: 429 924
StuI AGG'CCT
Cuts at: 0 508 1353
Size: 508 845
StyI C'CwwG_G
Cuts at: 0 509 515 1353
Size: 509 6 838
Fragments arranged by size:
838 509 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 27 481 489 1353
Size: 27 454 8 864
Fragments arranged by size:
864 454 27 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 588 620 1353
Size: 588 32 733
Fragments arranged by size:
733 588 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 325 1353
Size: 325 1028
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AleI AlwNI ApaI
ApoI BanII BbsI BbvCI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BstBI BstEII BstXI BstYI BtgI BtsI EaeI EarI
EcoICRI Eco57MI EcoO109I FspI FspAI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NcoI NspI PfoI PmlI
PpuMI PspOMI PstI SacI SanDI SfcI SnaBI StuI
StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AlfI AloI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BbeI BcgI BciVI BclI BfrBI BglII BmgBI
BmtI BpuEI BsaBI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrGI BssHII BssSI BstAPI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoRI EcoRV FalI FseI HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI