Restriction map for
pFN21AB3726
(Circular) (Six-base) MAPSORT of: pFN21AB3726.seq Check: 9878 from: 1 to: 7282
pFN21AB3726 7282 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4290 4290
Size: 7282
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4485 5092 278
Size: 53 83 186 936 2949 607 2468
Fragments arranged by size:
2949 2468 936 607 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4625 1937
Size: 2688 4594
Acc65I G'GTAC_C
Cuts at: 4603 4603
Size: 7282
AclI AA'CG_TT
Cuts at: 1563 5409 5782 1563
Size: 3846 373 3063
Fragments arranged by size:
3846 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2053 2384 2786 3023 3245 3923 5345 6729
Size: 421 331 402 237 222 678 1422 1384
Cuts at: 6729 6997 1632
Size: 268 1917
Fragments arranged by size:
1917 1422 1384 678 421 402 331 268
237 222
AfeI AGC'GCT
Cuts at: 3974 3974
Size: 7282
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7085
AflIII A'CryG_T
Cuts at: 1203 2685 6181 7158 1203
Size: 1482 3496 977 1327
Fragments arranged by size:
3496 1482 1327 977
AgeI A'CCGG_T
Cuts at: 6113 6113
Size: 7282
AhdI GACnn_n'nnGTC
Cuts at: 3496 4471 6011 3496
Size: 975 1540 4767
Fragments arranged by size:
4767 1540 975
AleI CACnn'nnGTG
Cuts at: 1166 2777 3836 4577 1166
Size: 1611 1059 741 3871
Fragments arranged by size:
3871 1611 1059 741
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7282
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2822 2854 2822
Size: 32 7250
AlwNI CAG_nnn'CTG
Cuts at: 2533 3697 4253 5025 6597 2533
Size: 1164 556 772 1572 3218
Fragments arranged by size:
3218 1572 1164 772 556
ApaI G_GGCC'C
Cuts at: 2124 2124
Size: 7282
ApaLI G'TGCA_C
Cuts at: 3110 5339 6495 3110
Size: 2229 1156 3897
Fragments arranged by size:
3897 2229 1156
ApoI r'AATT_y
Cuts at: 1493 3866 3878 4591 4817 4969 7078 1493
Size: 2373 12 713 226 152 2109 1697
Fragments arranged by size:
2373 2109 1697 713 226 152 12
AseI AT'TA_AT
Cuts at: 160 1032 5836 160
Size: 872 4804 1606
Fragments arranged by size:
4804 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
AvaI C'yCGr_G
Cuts at: 1870 1943 2293 3083 4190 4607 1870
Size: 73 350 790 1107 417 4545
Fragments arranged by size:
4545 1107 790 417 350 73
BamHI G'GATC_C
Cuts at: 3288 3434 3702 4612 4979 3288
Size: 146 268 910 367 5591
Fragments arranged by size:
5591 910 367 268 146
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3982 4603 6058 618
Size: 325 217 627 2195 621 1455 1842
Fragments arranged by size:
2195 1842 1455 627 621 325 217
BanII G_rGCy'C
Cuts at: 1391 2124 2135 2948 3410 3420 3651 3689 4365
Size: 733 11 813 462 10 231 38 676
Cuts at: 4365 4601 1391
Size: 236 4072
Fragments arranged by size:
4072 813 733 676 462 236 231 38
11 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2959 3014 3928 4087 928
Size: 971 1060 55 914 159 4123
Fragments arranged by size:
4123 1060 971 914 159 55
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5473 5507 1903
Size: 34 3536 34 3678
Fragments arranged by size:
3678 3536 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5174 6384 5174
Size: 1210 6072
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7282
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2025 5893 136
Size: 107 122 71 631 605 353 3868 1525
Fragments arranged by size:
3868 1525 631 605 353 122 107 71
BglII A'GATC_T
Cuts at: 2316 2634 3153 7277 2316
Size: 318 519 4124 2321
Fragments arranged by size:
4124 2321 519 318
BlpI GC'TnA_GC
Cuts at: 2015 2183 4699 2015
Size: 168 2516 4598
Fragments arranged by size:
4598 2516 168
Bme1580I G_kGCm'C
Cuts at: 1790 2124 2480 3114 3987 5343 6499 1790
Size: 334 356 634 873 1356 1156 2573
Fragments arranged by size:
2573 1356 1156 873 634 356 334
BmgBI CAC'GTC
Cuts at: 1318 2688 2965 3838 4579 1318
Size: 1370 277 873 741 4021
Fragments arranged by size:
4021 1370 873 741 277
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3685 5013 5971
Size: 416 53 83 399 24 2268 1328 958
Cuts at: 5971 442
Size: 1753
Fragments arranged by size:
2268 1753 1328 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 2609 3155 3374 3404 3486 3602 3701
Size: 1052 318 546 219 30 82 116 99
Cuts at: 3701 5942 1239
Size: 2241 2579
Fragments arranged by size:
2579 2241 1052 546 318 219 116 99
82 30
Bpu10I CC'TnA_GC
Cuts at: 3404 3449 3404
Size: 45 7237
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3149 4070 4151 4532 5407 6272 6570 3149
Size: 921 81 381 875 865 298 3861
Fragments arranged by size:
3861 921 875 865 381 298 81
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2132 2408 3809 4414 5945 882
Size: 617 633 276 1401 605 1531 2219
Fragments arranged by size:
2219 1531 1401 633 617 605 276
BsaAI yAC'GTr
Cuts at: 493 1204 5073 493
Size: 711 3869 2702
Fragments arranged by size:
3869 2702 711
BsaBI GATnn'nnATC
Cuts at: 3152 7276 3152
Size: 4124 3158
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2214 3933 4482 5089
Size: 53 83 186 936 681 1719 549 607
Cuts at: 5089 5471 6106 275
Size: 382 635 1451
Fragments arranged by size:
1719 1451 936 681 635 607 549 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5715 6113 6387 6534 6945 5715
Size: 398 274 147 411 6052
Fragments arranged by size:
6052 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3638 3668 1178
Size: 30 165 30 2235 30 4792
Fragments arranged by size:
4792 2235 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 3677 4025 4049 4190 4496 1186
Size: 937 1554 348 24 141 306 3972
Fragments arranged by size:
3972 1554 937 348 306 141 24
BseYI C'CCAG_C
Cuts at: 2385 4219 6485 6981 2385
Size: 1834 2266 496 2686
Fragments arranged by size:
2686 2266 1834 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2626 1163
Size: 573 890 5819
Fragments arranged by size:
5819 890 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4732 5493 6521 1540
Size: 275 186 2731 761 1028 2301
Fragments arranged by size:
2731 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2397 2744 2948 3114 3410 3420 3429 5343 5428
Size: 347 204 166 296 10 9 1914 85
Cuts at: 5428 6499 2397
Size: 1071 3180
Fragments arranged by size:
3180 1914 1071 347 296 204 166 85
10 9
BsmI GAATG_Cn'
Cuts at: 4793 4886 4793
Size: 93 7189
BsmBI CGTCTCn'nnnn_
Cuts at: 2221 2891 2221
Size: 670 6612
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2135 2397 2480 2744 2948 3114
Size: 399 334 11 262 83 264 204 166
Cuts at: 3114 3410 3420 3429 3651 3689 3987 4365 4601
Size: 296 10 9 222 38 298 378 236
Cuts at: 4601 5343 5428 6499 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 378 334 298 296
264 262 236 222 204 166 85 83
38 11 10 9
BspEI T'CCGG_A
Cuts at: 6945 6945
Size: 7282
BspHI T'CATG_A
Cuts at: 5171 6901 5171
Size: 1730 5552
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2769 4290 4483 4524 4637 844
Size: 1925 1521 193 41 113 3489
Fragments arranged by size:
3489 1925 1521 193 113 41
BsrBI CCG'CTC
Cuts at: 1394 1823 2014 4198 4290 5169 1394
Size: 429 191 2184 92 879 3507
Fragments arranged by size:
3507 2184 879 429 191 92
BsrDI GCAATG_nn'
Cuts at: 65 1248 5777 5951 65
Size: 1183 4529 174 1396
Fragments arranged by size:
4529 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3929 5926 6113 1700
Size: 110 2119 1997 187 2869
Fragments arranged by size:
2869 2119 1997 187 110
BsrGI T'GTAC_A
Cuts at: 96 2553 3457 96
Size: 2457 904 3921
Fragments arranged by size:
3921 2457 904
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7282
BssSI C'ACGA_G
Cuts at: 5342 6354 5342
Size: 1012 6270
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7282
BstBI TT'CG_AA
Cuts at: 6086 6086
Size: 7282
BstEII G'GTnAC_C
Cuts at: 1184 2506 1184
Size: 1322 5960
BstXI CCAn_nnnn'nTGG
Cuts at: 4579 4579
Size: 7282
BstYI r'GATC_y
Cuts at: 1972 2316 2430 2448 2634 3153 3288 3385 3434
Size: 344 114 18 186 519 135 97 49
Cuts at: 3434 3702 3789 4612 4979 5364 5381 6833 6919
Size: 268 87 823 367 385 17 1452 86
Cuts at: 6919 6931 7277 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 823 519 385 367 346 344
268 186 135 114 97 87 86 49
18 17 12
Bsu36I CC'TnA_GG
Cuts at: 2232 3698 2232
Size: 1466 5816
BtgI C'CryG_G
Cuts at: 513 1065 2004 2511 2697 3810 4068 513
Size: 552 939 507 186 1113 258 3727
Fragments arranged by size:
3727 1113 939 552 507 258 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4138 4222 487
Size: 683 56 2912 84 3547
Fragments arranged by size:
3547 2912 683 84 56
BtsI GCAGTG_nn'
Cuts at: 2883 4007 4798 5592 5612 2883
Size: 1124 791 794 20 4553
Fragments arranged by size:
4553 1124 794 791 20
ClaI AT'CG_AT
Cuts at: 1554 2154 1554
Size: 600 6682
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7247
DraI TTT'AAA
Cuts at: 4586 4586
Size: 7282
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2504 6289 809
Size: 507 1188 3785 1802
Fragments arranged by size:
3785 1802 1188 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2304 2535 3060
Size: 54 1425 50 275 18 474 231 525
Cuts at: 3060 4065 4729 5618 7247 8
Size: 1005 664 889 1629 43
Fragments arranged by size:
1629 1425 1005 889 664 525 474 275
231 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4729 1537
Size: 275 2917 4090
Fragments arranged by size:
4090 2917 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2307 2528 3899 4016 4328 5212 1353
Size: 954 221 1371 117 312 884 3423
Fragments arranged by size:
3423 1371 954 884 312 221 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5869 6243 6389 387
Size: 1064 138 4280 374 146 1280
Fragments arranged by size:
4280 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2946 3408 3418 2946
Size: 462 10 6810
Fragments arranged by size:
6810 462 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2291 2384 2609 2786 3023 3155
Size: 393 421 238 93 225 177 237 132
Cuts at: 3155 3245 3374 3404 3486 3602 3701 3923 5345
Size: 90 129 30 82 116 99 222 1422
Cuts at: 5345 5942 6729 6997 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1422 787 597 421 393 268 238
237 225 222 177 132 129 116 99
93 90 82 30
EcoO109I rG'GnC_Cy
Cuts at: 2026 2120 2121 2660 3909 4501 2026
Size: 94 1 539 1249 592 4807
Fragments arranged by size:
4807 1249 592 539 94 1
EcoRI G'AATT_C
Cuts at: 4591 4969 4591
Size: 378 6904
EcoRV GAT'ATC
Cuts at: 4056 4056
Size: 7282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2938 4111 4192 2938
Size: 1173 81 6028
Fragments arranged by size:
6028 1173 81
FspI TGC'GCA
Cuts at: 5788 5788
Size: 7282
HaeII r_GCGC'y
Cuts at: 1678 2190 3223 3976 4085 6429 1678
Size: 512 1033 753 109 2344 2531
Fragments arranged by size:
2531 2344 1033 753 512 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2201 2233 3280 3312 3482
Size: 32 135 32 629 32 1047 32 170
Cuts at: 3482 3514 4457 4489 5923 5955 5997 6029 1373
Size: 32 943 32 1434 32 42 32 2626
Fragments arranged by size:
2626 1434 1047 943 629 170 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3742 4626 4872 669
Size: 988 281 1804 884 246 3079
Fragments arranged by size:
3079 1804 988 884 281 246
HindIII A'AGCT_T
Cuts at: 748 3446 748
Size: 2698 4584
HpaI GTT'AAC
Cuts at: 4872 4872
Size: 7282
KpnI G_GTAC'C
Cuts at: 4607 4607
Size: 7282
MfeI C'AATT_G
Cuts at: 4881 4881
Size: 7282
MluI A'CGCG_T
Cuts at: 7158 7158
Size: 7282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2640 6396 6580 1305
Size: 1335 3756 184 2007
Fragments arranged by size:
3756 2007 1335 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2306 2537 4067 10
Size: 54 1425 343 474 231 1530 3225
Fragments arranged by size:
3225 1530 1425 474 343 231 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2777 3146 3836 4577 5240 5599 5758
Size: 648 1611 369 690 741 663 359 159
Cuts at: 5758 518
Size: 2042
Fragments arranged by size:
2042 1611 741 690 663 648 369 359
159
MspA1I CmG'CkG
Cuts at: 1606 2332 2597 2800 2908 3644 3796 4698 5375
Size: 726 265 203 108 736 152 902 677
Cuts at: 5375 6523 6768 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 902 736 726 677 265 245
203 152 108
NcoI C'CATG_G
Cuts at: 513 1065 2004 2511 2697 513
Size: 552 939 507 186 5098
Fragments arranged by size:
5098 939 552 507 186
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7282
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7282
NotI GC'GGCC_GC
Cuts at: 4729 4729
Size: 7282
NspI r_CATG'y
Cuts at: 1132 1229 4640 6185 1132
Size: 97 3411 1545 2229
Fragments arranged by size:
3411 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7282
PciI A'CATG_T
Cuts at: 6181 6181
Size: 7282
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2533 3133 3825 1108
Size: 1425 600 692 4565
Fragments arranged by size:
4565 1425 692 600
PfoI T'CCnGG_A
Cuts at: 2042 2433 2948 3502 3633 4179 2042
Size: 391 515 554 131 546 5145
Fragments arranged by size:
5145 554 546 515 391 131
PmeI GTTT'AAAC
Cuts at: 4586 4586
Size: 7282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3233 3265 5351 5383 6864 6896 3233
Size: 32 2086 32 1481 32 3619
Fragments arranged by size:
3619 2086 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2660 3909 4501 2660
Size: 1249 592 5441
Fragments arranged by size:
5441 1249 592
PshAI GACnn'nnGTC
Cuts at: 1613 3737 3932 1613
Size: 2124 195 4963
Fragments arranged by size:
4963 2124 195
PsiI TTA'TAA
Cuts at: 4852 4852
Size: 7282
PspOMI G'GGCC_C
Cuts at: 2120 2120
Size: 7282
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4547 4579 4547
Size: 32 7250
PstI C_TGCA'G
Cuts at: 830 2808 2878 3019 4521 4634 830
Size: 1978 70 141 1502 113 3478
Fragments arranged by size:
3478 1978 1502 141 113 70
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7282
PvuII CAG'CTG
Cuts at: 2332 2597 2908 3796 2332
Size: 265 311 888 5818
Fragments arranged by size:
5818 888 311 265
RsrII CG'GwC_CG
Cuts at: 4207 4207
Size: 7282
SacI G_AGCT'C
Cuts at: 2948 3410 3420 2948
Size: 462 10 6810
Fragments arranged by size:
6810 462 10
SalI G'TCGA_C
Cuts at: 1936 4624 1936
Size: 2688 4594
SbfI CC_TGCA'GG
Cuts at: 4634 4634
Size: 7282
ScaI AGT'ACT
Cuts at: 5530 7242 5530
Size: 1712 5570
SexAI A'CCwGG_T
Cuts at: 3066 3066
Size: 7282
SfcI C'TryA_G
Cuts at: 826 1045 2059 2804 2874 3015 3374 4048 4517
Size: 219 1014 745 70 141 359 674 469
Cuts at: 4517 4630 5765 6446 6637 826
Size: 113 1135 681 191 1471
Fragments arranged by size:
1471 1135 1014 745 681 674 469 359
219 191 141 113 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7282
SmaI CCC'GGG
Cuts at: 1872 4609 1872
Size: 2737 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3164 4085 4166 4547 5386 6287
Size: 197 926 1221 921 81 381 839 901
Cuts at: 6287 6549 820
Size: 262 1553
Fragments arranged by size:
1553 1221 926 921 901 839 381 262
197 81
SnaBI TAC'GTA
Cuts at: 493 5073 493
Size: 4580 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7282
SphI G_CATG'C
Cuts at: 1132 4640 1132
Size: 3508 3774
SspI AAT'ATT
Cuts at: 5 52 5206 5
Size: 47 5154 2081
Fragments arranged by size:
5154 2081 47
StuI AGG'CCT
Cuts at: 1517 2268 3395 1517
Size: 751 1127 5404
Fragments arranged by size:
5404 1127 751
StyI C'CwwG_G
Cuts at: 513 1065 2004 2481 2511 2664 2673 2697 2889
Size: 552 939 477 30 153 9 24 192
Cuts at: 2889 3543 3735 3978 4260 4721 513
Size: 654 192 243 282 461 3074
Fragments arranged by size:
3074 939 654 552 477 461 282 243
192 192 153 30 24 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4224 5309 5326 5479 5664 6109 325
Size: 1255 2644 1085 17 153 185 445 1498
Fragments arranged by size:
2644 1498 1255 1085 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2553 3457 3570
Size: 274 80 33 51 1443 576 904 113
Cuts at: 3570 4173 4563 5528 7240 96
Size: 603 390 965 1712 138
Fragments arranged by size:
1712 1443 965 904 603 576 390 274
138 113 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3638 3670 7242 7274 3638
Size: 32 3572 32 3646
Fragments arranged by size:
3646 3572 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2729 3913 1316
Size: 1413 1184 4685
Fragments arranged by size:
4685 1413 1184
XbaI T'CTAG_A
Cuts at: 4618 4618
Size: 7282
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3299 3299
Size: 7282
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7282
XmaI C'CCGG_G
Cuts at: 1870 4607 1870
Size: 2737 4545
XmnI GAAnn'nnTTC
Cuts at: 3248 3363 5411 3248
Size: 115 2048 5119
Fragments arranged by size:
5119 2048 115
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4483 5090 276
Size: 53 83 186 936 2949 607 2468
Fragments arranged by size:
2949 2468 936 607 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI
BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI SacII SanDI
SapI SfiI SfoI SrfI SwaI