Restriction map for
pFN21AB3717
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
Aor51HI ApaI ApoI AscI Asp700I
Asp718I AviII AvrII BalI BanI
BbeI BbrPI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BssHII BssSI BstAPI
BstUI CelII ClaI CpoI DraI
DraIII DrdI EaeI EagI EarI
EclXI Eco47III Eco52I EcoNI EcoRI
EcoT22I FseI FspI Hin1I HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmlI SnaBI SpeI Sse8387I
SwaI Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AhdI 1260 GACGGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 497 AGCT AG^CT
AspEI 1260 GACGGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 30 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1384 GGATCC G^GATCC
BanII 957 GGGCTC G[GA]GC[CT]^C
BclI 1298 TGATCA T^GATCA
BpmI 650 CTGGAG CTGGAG(16/14)
BseRI 217 GAGGAG GAGGAG(10/8)
BsmBI 1258 GAGACG CGTCTC(1/5)
Bsp1407I 858 TGTACA T^GTACA
BspMI 821 GCAGGT ACCTGC(4/8)
BsrFI 925 GCCGGC [GA]^CCGG[CT]
BsrGI 858 TGTACA T^GTACA
BstBI 1427 TTCGAA TT^CGAA
BstEII 51 GGTGACC G^GT[ACGT]ACC
BstPI 51 GGTGACC G^GT[ACGT]ACC
BstXI 366 CCAATCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1330 GCGC GCG^C
Cfr10I 925 GCCGGC [GA]^CCGG[CT]
DraII 791 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 1260 GACGGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 791 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 51 GGTGACC G^GT[ACGT]ACC
EcoRV 580 GATATC GAT^ATC
FauI 25 GCGGG CCCGC(4/6)
FbaI 1298 TGATCA T^GATCA
HaeII 1329 GGCGCT [GA]GCGC^[CT]
HgaI 59 GCGTC GACGC(5/10)
HhaI 1330 GCGC GCG^C
HinP1I 1330 GCGC G^CGC
MspA1I 1403 CAGCGG C[AC]G^C[GT]G
NaeI 925 GCCGGC GCC^GGC
NgoMIV 925 GCCGGC G^CCGGC
NspV 1427 TTCGAA TT^CGAA
PflFI 30 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 791 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 893 CTGCAG CTGCA^G
SalI 1268 GTCGAC G^TCGAC
SfuI 1427 TTCGAA TT^CGAA
SmaI 77 CCCGGG CCC^GGG
SspBI 858 TGTACA T^GTACA
StuI 344 AGGCCT AGG^CCT
TfiI 760 GATTC G^A[AT]TC
Tth111I 30 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 77 CCCGGG C^CCGGG
XmaI 77 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AciI 25 GCGG CCGC(-3/-1)
1405 GCGG
Alw44I 445 GTGCAC G^TGCAC
995 GTGCAC
AlwNI 862 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
978 CAGCATCTG
AocI 453 CCTCAGG CC^T[ACGT]AGG
940 CCTGAGG
ApaLI 445 GTGCAC G^TGCAC
995 GTGCAC
AseI 742 ATTAAT AT^TAAT
1105 ATTAAT
AsnI 742 ATTAAT AT^TAAT
1105 ATTAAT
AvaI 77 CCCGGG C^[CT]CG[GA]G
242 CTCGGG
AvaII 437 GGTCC G^G[AT]CC
792 GGTCC
BcnI 77 CCCGG CC^[GC]GG
78 CCGGG
BsoBI 77 CCCGGG C^[CT]CG[GA]G
242 CTCGGG
Bst1107I 180 GTATAC GTA^TAC
935 GTATAC
BstF5I 1392 GGATG GGATG(2/0)
1407 GGATG
BstYI 221 AGATCC [GA]^GATC[CT]
1384 GGATCC
BstZ17I 180 GTATAC GTA^TAC
935 GTATAC
Bsu36I 453 CCTCAGG CC^T[ACGT]AGG
940 CCTGAGG
DsaI 1208 CCATGG C^C[GA][CT]GG
1388 CCATGG
Eco81I 453 CCTCAGG CC^T[ACGT]AGG
940 CCTGAGG
EcoT14I 1208 CCATGG C^C[AT][AT]GG
1388 CCATGG
FokI 1392 GGATG GGATG(9/13)
1407 GGATG
HapII 78 CCGG C^CGG
926 CCGG
HincII 517 GTTGAC GT[CT]^[GA]AC
1268 GTCGAC
HindII 517 GTTGAC GT[CT]^[GA]AC
1268 GTCGAC
HpaII 78 CCGG C^CGG
926 CCGG
MflI 221 AGATCC [GA]^GATC[CT]
1384 GGATCC
MslI 1002 CATCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1320 CATCATCATG
MspI 78 CCGG C^CGG
926 CCGG
MwoI 5 GCCGATGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
165 GCCCCCTCTGC
NciI 77 CCCGG CC^[GC]GG
78 CCGGG
NcoI 1208 CCATGG C^CATGG
1388 CCATGG
NspI 1064 GCATGC [GA]CATG^[CT]
1369 GCATGC
PshAI 54 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1271 GACTGAAGTC
PshBI 742 ATTAAT AT^TAAT
1105 ATTAAT
SfaNI 8 GATGC GCATC(5/9)
980 GCATC
SphI 1064 GCATGC GCATG^C
1369 GCATGC
StyI 1208 CCATGG C^C[AT][AT]GG
1388 CCATGG
XhoII 221 AGATCC [GA]^GATC[CT]
1384 GGATCC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccI AfaI AlwI AspHI BbsI
BbvI BmyI BpuAI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
HaeIII HinfI HphI ItaI MaeII
MaeIII MboI MboII MnlI MseI
MvaI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfcI
SspI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I