Restriction map for
pFN21AB3717
(Circular) (Six-base) MAPSORT of: pFN21AB3717.seq Check: 8603 from: 1 to: 6139
pFN21AB3717 6139 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3949 278
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2103 2186 2941 3274 3482 1937
Size: 166 83 755 333 208 4594
Fragments arranged by size:
4594 755 333 208 166 83
Acc65I G'GTAC_C
Cuts at: 3460 3460
Size: 6139
AclI AA'CG_TT
Cuts at: 1563 4266 4639 1563
Size: 2703 373 3063
Fragments arranged by size:
3063 2703 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3299 4202 5586 5854 1632
Size: 1667 903 1384 268 1917
Fragments arranged by size:
1917 1667 1384 903 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5942
AflIII A'CryG_T
Cuts at: 1203 5038 6015 1203
Size: 3835 977 1327
Fragments arranged by size:
3835 1327 977
AgeI A'CCGG_T
Cuts at: 4970 4970
Size: 6139
AhdI GACnn_n'nnGTC
Cuts at: 3270 4868 3270
Size: 1598 4541
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6139
AlwNI CAG_nnn'CTG
Cuts at: 2872 2988 3882 5454 2872
Size: 116 894 1572 3557
Fragments arranged by size:
3557 1572 894 116
ApaLI G'TGCA_C
Cuts at: 2450 3000 4196 5352 2450
Size: 550 1196 1156 3237
Fragments arranged by size:
3237 1196 1156 550
ApoI r'AATT_y
Cuts at: 1493 3448 3674 3826 5935 1493
Size: 1955 226 152 2109 1697
Fragments arranged by size:
2109 1955 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2748 3111 4693 160
Size: 872 1716 363 1582 1606
Fragments arranged by size:
1716 1606 1582 872 363
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
AvaI C'yCGr_G
Cuts at: 1870 1943 2082 2247 3464 1870
Size: 73 139 165 1217 4545
Fragments arranged by size:
4545 1217 165 139 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2095 2128 2095
Size: 33 6106
BamHI G'GATC_C
Cuts at: 3389 3469 3836 3389
Size: 80 367 5692
Fragments arranged by size:
5692 367 80
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3460 4915 618
Size: 325 217 627 1673 1455 1842
Fragments arranged by size:
1842 1673 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2966 3458 1391
Size: 1575 492 4072
Fragments arranged by size:
4072 1575 492
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2080 2667 2800 3093 928
Size: 971 181 587 133 293 3974
Fragments arranged by size:
3974 971 587 293 181 133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4330 4364 1903
Size: 34 2393 34 3678
Fragments arranged by size:
3678 2393 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4031 5241 4031
Size: 1210 4929
BclI T'GATC_A
Cuts at: 1548 3303 1548
Size: 1755 4384
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4750 136
Size: 107 122 71 631 605 3078 1525
Fragments arranged by size:
3078 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6134 6134
Size: 6139
BlpI GC'TnA_GC
Cuts at: 3556 3556
Size: 6139
Bme1580I G_kGCm'C
Cuts at: 1790 2454 3004 4200 5356 1790
Size: 664 550 1196 1156 2573
Fragments arranged by size:
2573 1196 1156 664 550
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3218 3870 4828
Size: 416 53 83 399 24 1801 652 958
Cuts at: 4828 442
Size: 1753
Fragments arranged by size:
1801 1753 958 652 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6139
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3443
Size: 6139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2676 4799 1239
Size: 1437 2123 2579
Fragments arranged by size:
2579 2123 1437
Bpu10I CC'TnA_GC
Cuts at: 2965 2965
Size: 6139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4264 5129 5427 4264
Size: 865 298 4976
Fragments arranged by size:
4976 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2234 2361 2376 4802 882
Size: 617 735 127 15 2426 2219
Fragments arranged by size:
2426 2219 735 617 127 15
BsaAI yAC'GTr
Cuts at: 493 1204 3930 493
Size: 711 2726 2702
Fragments arranged by size:
2726 2702 711
BsaBI GATnn'nnATC
Cuts at: 6133 6133
Size: 6139
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3946 4328 4963 275
Size: 53 83 186 936 2413 382 635 1451
Fragments arranged by size:
2413 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4572 4970 5244 5391 5802 4572
Size: 398 274 147 411 4909
Fragments arranged by size:
4909 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2628 2658 1178
Size: 30 165 30 1225 30 4659
Fragments arranged by size:
4659 1225 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 1186
Size: 1051 5088
BseYI C'CCAG_C
Cuts at: 3337 5342 5838 3337
Size: 2005 496 3638
Fragments arranged by size:
3638 2005 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5566
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3589 4350 5378 1540
Size: 275 186 1588 761 1028 2301
Fragments arranged by size:
2301 1588 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2454 3004 3424 4200 4285 5356 2454
Size: 550 420 776 85 1071 3237
Fragments arranged by size:
3237 1071 776 550 420 85
BsmI GAATG_Cn'
Cuts at: 3650 3743 3650
Size: 93 6046
BsmBI CGTCTCn'nnnn_
Cuts at: 3257 3257
Size: 6139
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2454 2966 3004 3424 3458 4200 4285
Size: 399 664 512 38 420 34 742 85
Cuts at: 4285 5356 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 664 512 420 399 85
38 34
BspEI T'CCGG_A
Cuts at: 5802 5802
Size: 6139
BspHI T'CATG_A
Cuts at: 4028 5758 4028
Size: 1730 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2817 3494 844
Size: 1973 677 3489
Fragments arranged by size:
3489 1973 677
BsrBI CCG'CTC
Cuts at: 1394 1823 4026 1394
Size: 429 2203 3507
Fragments arranged by size:
3507 2203 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4634 4808 65
Size: 1183 3386 174 1396
Fragments arranged by size:
3386 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2930 4783 4970 1700
Size: 110 1120 1853 187 2869
Fragments arranged by size:
2869 1853 1120 187 110
BsrGI T'GTAC_A
Cuts at: 96 2863 96
Size: 2767 3372
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6139
BssSI C'ACGA_G
Cuts at: 4199 5211 4199
Size: 1012 5127
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6139
BstBI TT'CG_AA
Cuts at: 3433 4943 3433
Size: 1510 4629
BstEII G'GTnAC_C
Cuts at: 1184 2056 1184
Size: 872 5267
BstXI CCAn_nnnn'nTGG
Cuts at: 2378 2378
Size: 6139
BstYI r'GATC_y
Cuts at: 1972 2226 3389 3469 3836 4221 4238 5690 5776
Size: 254 1163 80 367 385 17 1452 86
Cuts at: 5776 5788 6134 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1163 385 367 346 254 86
80 17 12
BstZ17I GTA'TAC
Cuts at: 2187 2942 2187
Size: 755 5384
Bsu36I CC'TnA_GG
Cuts at: 2459 2946 2459
Size: 487 5652
BtgI C'CryG_G
Cuts at: 513 1065 2004 3213 3393 513
Size: 552 939 1209 180 3259
Fragments arranged by size:
3259 1209 939 552 180
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5400
Fragments arranged by size:
5400 683 56
BtsI GCAGTG_nn'
Cuts at: 3655 4449 4469 3655
Size: 794 20 5325
Fragments arranged by size:
5325 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2670 2705 529
Size: 35 2106 35 3963
Fragments arranged by size:
3963 2106 35 35
DraI TTT'AAA
Cuts at: 3443 3443
Size: 6139
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5146 809
Size: 507 3830 1802
Fragments arranged by size:
3830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3586 4475 6104
Size: 54 1425 50 275 18 1756 889 1629
Cuts at: 6104 8
Size: 43
Fragments arranged by size:
1756 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3586 1537
Size: 275 1774 4090
Fragments arranged by size:
4090 1774 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4069 1353
Size: 2716 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4726 5100 5246 387
Size: 1064 138 3137 374 146 1280
Fragments arranged by size:
3137 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2676 3299 4202 4799 5586 5854 1239
Size: 393 1044 623 903 597 787 268 1524
Fragments arranged by size:
1524 1044 903 787 623 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2797 2797
Size: 6139
EcoRI G'AATT_C
Cuts at: 3448 3826 3448
Size: 378 5761
EcoRV GAT'ATC
Cuts at: 2587 2587
Size: 6139
FspI TGC'GCA
Cuts at: 4645 4645
Size: 6139
HaeII r_GCGC'y
Cuts at: 1678 3338 5286 1678
Size: 1660 1948 2531
Fragments arranged by size:
2531 1948 1660
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3256 3288 4780 4812 4854
Size: 32 135 32 1684 32 1492 32 42
Cuts at: 4854 4886 1373
Size: 32 2626
Fragments arranged by size:
2626 1684 1492 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2524 3275 3483 3729 669
Size: 988 281 586 751 208 246 3079
Fragments arranged by size:
3079 988 751 586 281 246 208
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6139
HpaI GTT'AAC
Cuts at: 3729 3729
Size: 6139
KpnI G_GTAC'C
Cuts at: 3464 3464
Size: 6139
MfeI C'AATT_G
Cuts at: 3738 3738
Size: 6139
MluI A'CGCG_T
Cuts at: 6015 6015
Size: 6139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2359 5253 5437 1305
Size: 1054 2894 184 2007
Fragments arranged by size:
2894 2007 1054 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4317
Fragments arranged by size:
4317 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3011 3329 4097 4456 4615 518
Size: 648 1845 318 768 359 159 2042
Fragments arranged by size:
2042 1845 768 648 359 318 159
MspA1I CmG'CkG
Cuts at: 1606 3410 3555 4232 5380 5625 1606
Size: 1804 145 677 1148 245 2120
Fragments arranged by size:
2120 1804 1148 677 245 145
NaeI GCC'GGC
Cuts at: 2932 2932
Size: 6139
NcoI C'CATG_G
Cuts at: 513 1065 2004 3213 3393 513
Size: 552 939 1209 180 3259
Fragments arranged by size:
3259 1209 939 552 180
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6139
NgoMIV G'CCGG_C
Cuts at: 2930 2930
Size: 6139
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6139
NotI GC'GGCC_GC
Cuts at: 3586 3586
Size: 6139
NspI r_CATG'y
Cuts at: 1132 1229 3073 3378 3497 5042 1132
Size: 97 1844 305 119 1545 2229
Fragments arranged by size:
2229 1844 1545 305 119 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6139
PciI A'CATG_T
Cuts at: 5038 5038
Size: 6139
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6139
PmeI GTTT'AAAC
Cuts at: 3443 3443
Size: 6139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 5721 5753 4208
Size: 32 1481 32 4594
Fragments arranged by size:
4594 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2797 2797
Size: 6139
PshAI GACnn'nnGTC
Cuts at: 1613 2063 3280 1613
Size: 450 1217 4472
Fragments arranged by size:
4472 1217 450
PsiI TTA'TAA
Cuts at: 3709 3709
Size: 6139
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2177 2209 2177
Size: 32 6107
PstI C_TGCA'G
Cuts at: 830 2902 3491 830
Size: 2072 589 3478
Fragments arranged by size:
3478 2072 589
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6139
SalI G'TCGA_C
Cuts at: 1936 3273 3481 1936
Size: 1337 208 4594
Fragments arranged by size:
4594 1337 208
SbfI CC_TGCA'GG
Cuts at: 3491 3491
Size: 6139
ScaI AGT'ACT
Cuts at: 4387 6099 4387
Size: 1712 4427
SfcI C'TryA_G
Cuts at: 826 1045 2100 2275 2898 3487 4622 5303 5494
Size: 219 1055 175 623 589 1135 681 191
Cuts at: 5494 826
Size: 1471
Fragments arranged by size:
1471 1135 1055 681 623 589 219 191
175
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6139
SmaI CCC'GGG
Cuts at: 1872 2084 3466 1872
Size: 212 1382 4545
Fragments arranged by size:
4545 1382 212
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4243 5144 5406 820
Size: 197 926 2300 901 262 1553
Fragments arranged by size:
2300 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3930 493
Size: 3437 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6139
SphI G_CATG'C
Cuts at: 1132 3073 3378 3497 1132
Size: 1941 305 119 3774
Fragments arranged by size:
3774 1941 305 119
SspI AAT'ATT
Cuts at: 5 52 2302 2416 2907 4063 5
Size: 47 2250 114 491 1156 2081
Fragments arranged by size:
2250 2081 1156 491 114 47
StuI AGG'CCT
Cuts at: 1517 2351 1517
Size: 834 5305
StyI C'CwwG_G
Cuts at: 513 1065 2004 3213 3393 3578 513
Size: 552 939 1209 180 185 3074
Fragments arranged by size:
3074 1209 939 552 185 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2790 3019 3090 3329 4166 4183 4336
Size: 1255 1210 229 71 239 837 17 153
Cuts at: 4336 4521 4966 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1210 837 445 239 229 185
153 71 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2863 4385 6097
Size: 274 80 33 51 1443 886 1522 1712
Cuts at: 6097 96
Size: 138
Fragments arranged by size:
1712 1522 1443 886 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6099 6131 6099
Size: 32 6107
Tth111I GACn'n_nGTC
Cuts at: 1316 2038 1316
Size: 722 5417
XbaI T'CTAG_A
Cuts at: 3475 3475
Size: 6139
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6139
XmaI C'CCGG_G
Cuts at: 1870 2082 3464 1870
Size: 212 1382 4545
Fragments arranged by size:
4545 1382 212
XmnI GAAnn'nnTTC
Cuts at: 4268 4268
Size: 6139
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3947 276
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BsiWI DraIII EcoICRI EcoNI FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI