(Circular) (Six-base) MAPSORT of: pFN21AB3683.seq Check: 9337 from: 1 to: 8227 pFN21AB3683 8227 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3456 3456 Size: 8227 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 6037 278 Size: 53 83 186 936 2245 2256 2468 Fragments arranged by size: 2468 2256 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3434 3871 4407 4816 5570 1937 Size: 1497 437 536 409 754 4594 Fragments arranged by size: 4594 1497 754 536 437 409 Acc65I G'GTAC_C Cuts at: 5548 5548 Size: 8227 AclI AA'CG_TT Cuts at: 1563 6354 6727 1563 Size: 4791 373 3063 Fragments arranged by size: 4791 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2232 2282 2408 3822 4405 6290 7674 7942 Size: 600 50 126 1414 583 1885 1384 268 Cuts at: 7942 1632 Size: 1917 Fragments arranged by size: 1917 1885 1414 1384 600 583 268 126 50 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8030 AflIII A'CryG_T Cuts at: 1203 4502 7126 8103 1203 Size: 3299 2624 977 1327 Fragments arranged by size: 3299 2624 1327 977 AgeI A'CCGG_T Cuts at: 7058 7058 Size: 8227 AhdI GACnn_n'nnGTC Cuts at: 4938 6956 4938 Size: 2018 6209 AleI CACnn'nnGTG Cuts at: 1166 5187 1166 Size: 4021 4206 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8227 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3093 3125 3093 Size: 32 8195 AlwNI CAG_nnn'CTG Cuts at: 5421 5970 7542 5421 Size: 549 1572 6106 Fragments arranged by size: 6106 1572 549 ApaLI G'TGCA_C Cuts at: 6284 7440 6284 Size: 1156 7071 ApoI r'AATT_y Cuts at: 1493 2274 2457 2683 2959 3232 3266 3460 3577 Size: 781 183 226 276 273 34 194 117 Cuts at: 3577 3632 3658 3669 4754 4805 5536 5762 5914 Size: 55 26 11 1085 51 731 226 152 Cuts at: 5914 8023 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1085 781 731 276 273 226 226 194 183 152 117 55 51 34 26 11 AseI AT'TA_AT Cuts at: 160 1032 2565 6781 160 Size: 872 1533 4216 1606 Fragments arranged by size: 4216 1606 1533 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8227 AvaI C'yCGr_G Cuts at: 1870 1943 5552 1870 Size: 73 3609 4545 Fragments arranged by size: 4545 3609 73 BamHI G'GATC_C Cuts at: 2542 5557 5924 2542 Size: 3015 367 4845 Fragments arranged by size: 4845 3015 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2741 5548 7003 618 Size: 325 217 627 954 2807 1455 1842 Fragments arranged by size: 2807 1842 1455 954 627 325 217 BanII G_rGCy'C Cuts at: 1391 5546 1391 Size: 4155 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2190 4383 928 Size: 971 291 2193 4772 Fragments arranged by size: 4772 2193 971 291 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6418 6452 1903 Size: 34 4481 34 3678 Fragments arranged by size: 4481 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3095 6119 7329 3095 Size: 3024 1210 3993 Fragments arranged by size: 3993 3024 1210 BclI T'GATC_A Cuts at: 1548 3367 3813 4147 4474 1548 Size: 1819 446 334 327 5301 Fragments arranged by size: 5301 1819 446 334 327 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3590 6838 136 Size: 107 122 71 631 605 1918 3248 1525 Fragments arranged by size: 3248 1918 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5237 8222 5237 Size: 2985 5242 BlpI GC'TnA_GC Cuts at: 2217 5644 2217 Size: 3427 4800 Bme1580I G_kGCm'C Cuts at: 1790 6288 7444 1790 Size: 4498 1156 2573 Fragments arranged by size: 4498 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8227 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3370 5299 5958 Size: 416 53 83 399 24 1953 1929 659 Cuts at: 5958 6916 442 Size: 958 1753 Fragments arranged by size: 1953 1929 1753 958 659 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3491 1056 Size: 2435 5792 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3417 4023 4554 6887 1239 Size: 2178 606 531 2333 2579 Fragments arranged by size: 2579 2333 2178 606 531 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2699 6352 7217 7515 2699 Size: 3653 865 298 3411 Fragments arranged by size: 3653 3411 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3071 3536 6890 882 Size: 617 1572 465 3354 2219 Fragments arranged by size: 3354 2219 1572 617 465 BsaAI yAC'GTr Cuts at: 493 1204 2361 4505 6018 493 Size: 711 1157 2144 1513 2702 Fragments arranged by size: 2702 2144 1513 1157 711 BsaBI GATnn'nnATC Cuts at: 2090 2511 3788 3842 5343 8221 2090 Size: 421 1277 54 1501 2878 2096 Fragments arranged by size: 2878 2096 1501 1277 421 54 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3778 6034 6416 7051 Size: 53 83 186 936 2245 2256 382 635 Cuts at: 7051 275 Size: 1451 Fragments arranged by size: 2256 2245 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6660 7058 7332 7479 7890 6660 Size: 398 274 147 411 6997 Fragments arranged by size: 6997 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4581 4611 1178 Size: 30 165 30 3178 30 4794 Fragments arranged by size: 4794 3178 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2181 2745 2991 1186 Size: 995 564 246 6422 Fragments arranged by size: 6422 995 564 246 BseYI C'CCAG_C Cuts at: 3191 3720 7430 7926 3191 Size: 529 3710 496 3492 Fragments arranged by size: 3710 3492 529 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5048 1163 Size: 573 3312 4342 Fragments arranged by size: 4342 3312 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5677 6438 7466 1540 Size: 275 186 3676 761 1028 2301 Fragments arranged by size: 3676 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2317 4652 6288 6373 7444 2317 Size: 2335 1636 85 1071 3100 Fragments arranged by size: 3100 2335 1636 1071 85 BsmI GAATG_Cn' Cuts at: 2537 3206 4958 5417 5460 5738 5831 2537 Size: 669 1752 459 43 278 93 4933 Fragments arranged by size: 4933 1752 669 459 278 93 43 BsmBI CGTCTCn'nnnn_ Cuts at: 3471 3769 4697 3471 Size: 298 928 7001 Fragments arranged by size: 7001 928 298 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2317 4652 5546 6288 6373 7444 1391 Size: 399 527 2335 894 742 85 1071 2174 Fragments arranged by size: 2335 2174 1071 894 742 527 399 85 BspEI T'CCGG_A Cuts at: 7890 7890 Size: 8227 BspHI T'CATG_A Cuts at: 4389 6116 7846 4389 Size: 1727 1730 4770 Fragments arranged by size: 4770 1730 1727 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3456 5582 844 Size: 2612 2126 3489 Fragments arranged by size: 3489 2612 2126 BsrBI CCG'CTC Cuts at: 1394 1823 6114 1394 Size: 429 4291 3507 Fragments arranged by size: 4291 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4038 4347 6722 6896 65 Size: 1183 2790 309 2375 174 1396 Fragments arranged by size: 2790 2375 1396 1183 309 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6871 7058 1700 Size: 110 5061 187 2869 Fragments arranged by size: 5061 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5088 96 Size: 4992 3235 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8227 BssSI C'ACGA_G Cuts at: 5187 5241 6287 7299 5187 Size: 54 1046 1012 6115 Fragments arranged by size: 6115 1046 1012 54 BstAPI GCAn_nnn'nTGC Cuts at: 794 3686 5421 794 Size: 2892 1735 3600 Fragments arranged by size: 3600 2892 1735 BstBI TT'CG_AA Cuts at: 7031 7031 Size: 8227 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8227 BstYI r'GATC_y Cuts at: 1972 2542 2884 3184 3252 3608 3739 3843 3967 Size: 570 342 300 68 356 131 104 124 Cuts at: 3967 5208 5237 5338 5557 5924 6309 6326 7778 Size: 1241 29 101 219 367 385 17 1452 Cuts at: 7778 7864 7876 8222 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1241 570 385 367 356 346 342 300 219 131 124 104 101 86 68 29 17 12 Bsu36I CC'TnA_GG Cuts at: 3764 3764 Size: 8227 BtgI C'CryG_G Cuts at: 513 1065 4411 5226 5293 513 Size: 552 3346 815 67 3447 Fragments arranged by size: 3447 3346 815 552 67 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7488 Fragments arranged by size: 7488 683 56 BtsI GCAGTG_nn' Cuts at: 5743 6537 6557 5743 Size: 794 20 7413 Fragments arranged by size: 7413 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8227 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2238 2273 5350 5385 529 Size: 35 1674 35 3077 35 3371 Fragments arranged by size: 3371 3077 1674 35 35 35 DraI TTT'AAA Cuts at: 3459 5531 3459 Size: 2072 6155 DraIII CAC_nnn'GTG Cuts at: 4091 4091 Size: 8227 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3430 4288 7234 809 Size: 507 2114 858 2946 1802 Fragments arranged by size: 2946 2114 1802 858 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3975 4344 5674 Size: 54 1425 50 275 18 2145 369 1330 Cuts at: 5674 6563 8192 8 Size: 889 1629 43 Fragments arranged by size: 2145 1629 1425 1330 889 369 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5674 1537 Size: 275 3862 4090 Fragments arranged by size: 4090 3862 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2744 3136 3908 6157 1353 Size: 1391 392 772 2249 3423 Fragments arranged by size: 3423 2249 1391 772 392 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6814 7188 7334 387 Size: 1064 138 5225 374 146 1280 Fragments arranged by size: 5225 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2232 2282 2408 3417 3822 4023 4405 Size: 393 600 50 126 1009 405 201 382 Cuts at: 4405 4554 6290 6887 7674 7942 1239 Size: 149 1736 597 787 268 1524 Fragments arranged by size: 1736 1524 1009 787 600 597 405 393 382 268 201 149 126 50 EcoO109I rG'GnC_Cy Cuts at: 5083 5198 5397 5083 Size: 115 199 7913 Fragments arranged by size: 7913 199 115 EcoRI G'AATT_C Cuts at: 2683 5536 5914 2683 Size: 2853 378 4996 Fragments arranged by size: 4996 2853 378 EcoRV GAT'ATC Cuts at: 4897 4897 Size: 8227 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2438 2438 Size: 8227 FspI TGC'GCA Cuts at: 6733 6733 Size: 8227 HaeII r_GCGC'y Cuts at: 1678 7374 1678 Size: 5696 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2419 2451 2654 2686 3169 Size: 32 135 32 847 32 203 32 483 Cuts at: 3169 3201 4197 4229 4924 4956 5026 5058 5170 Size: 32 996 32 695 32 70 32 112 Cuts at: 5170 5202 5222 5254 6868 6900 6942 6974 1373 Size: 32 20 32 1614 32 42 32 2626 Fragments arranged by size: 2626 1614 996 847 695 483 203 135 112 70 42 32 32 32 32 32 32 32 32 32 32 32 32 20 HincII GTy'rAC Cuts at: 669 1657 1938 2478 4817 5023 5571 5817 669 Size: 988 281 540 2339 206 548 246 3079 Fragments arranged by size: 3079 2339 988 548 540 281 246 206 HindIII A'AGCT_T Cuts at: 748 2925 748 Size: 2177 6050 HpaI GTT'AAC Cuts at: 5023 5817 5023 Size: 794 7433 KpnI G_GTAC'C Cuts at: 5552 5552 Size: 8227 MfeI C'AATT_G Cuts at: 3929 4338 5826 3929 Size: 409 1488 6330 Fragments arranged by size: 6330 1488 409 MluI A'CGCG_T Cuts at: 8103 8103 Size: 8227 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2868 3586 3706 7341 7525 1305 Size: 1563 718 120 3635 184 2007 Fragments arranged by size: 3635 2007 1563 718 184 120 MscI TGG'CCA Cuts at: 10 64 1489 1832 3977 4346 10 Size: 54 1425 343 2145 369 3891 Fragments arranged by size: 3891 2145 1425 369 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2226 2816 3057 3794 5187 5406 5410 Size: 648 1060 590 241 737 1393 219 4 Cuts at: 5410 6185 6544 6703 518 Size: 775 359 159 2042 Fragments arranged by size: 2042 1393 1060 775 737 648 590 359 241 219 159 4 MspA1I CmG'CkG Cuts at: 1606 2134 3199 4403 4413 5643 6320 7468 7713 Size: 528 1065 1204 10 1230 677 1148 245 Cuts at: 7713 1606 Size: 2120 Fragments arranged by size: 2120 1230 1204 1148 1065 677 528 245 10 NcoI C'CATG_G Cuts at: 513 1065 5226 5293 513 Size: 552 4161 67 3447 Fragments arranged by size: 4161 3447 552 67 NdeI CA'TA_TG Cuts at: 387 5163 5407 387 Size: 4776 244 3207 Fragments arranged by size: 4776 3207 244 NheI G'CTAG_C Cuts at: 1052 3487 1052 Size: 2435 5792 NotI GC'GGCC_GC Cuts at: 5674 5674 Size: 8227 NspI r_CATG'y Cuts at: 1132 1229 2082 2225 2722 4515 4532 5469 5585 Size: 97 853 143 497 1793 17 937 116 Cuts at: 5585 7130 1132 Size: 1545 2229 Fragments arranged by size: 2229 1793 1545 937 853 497 143 116 97 17 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8227 PciI A'CATG_T Cuts at: 7126 7126 Size: 8227 PflMI CCAn_nnn'nTGG Cuts at: 1108 4796 1108 Size: 3688 4539 PmeI GTTT'AAAC Cuts at: 5531 5531 Size: 8227 PmlI CAC'GTG Cuts at: 4505 4505 Size: 8227 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6296 6328 7809 7841 6296 Size: 32 1481 32 6682 Fragments arranged by size: 6682 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5083 5198 5397 5083 Size: 115 199 7913 Fragments arranged by size: 7913 199 115 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8227 PsiI TTA'TAA Cuts at: 5797 5797 Size: 8227 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5081 5113 5081 Size: 32 8195 PstI C_TGCA'G Cuts at: 830 3682 5579 830 Size: 2852 1897 3478 Fragments arranged by size: 3478 2852 1897 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8227 PvuII CAG'CTG Cuts at: 2134 4403 2134 Size: 2269 5958 SacII CC_GC'GG Cuts at: 4414 4414 Size: 8227 SalI G'TCGA_C Cuts at: 1936 4815 5569 1936 Size: 2879 754 4594 Fragments arranged by size: 4594 2879 754 SbfI CC_TGCA'GG Cuts at: 5579 5579 Size: 8227 ScaI AGT'ACT Cuts at: 2704 6475 8187 2704 Size: 3771 1712 2744 Fragments arranged by size: 3771 2744 1712 SfcI C'TryA_G Cuts at: 826 1045 2196 2204 3561 3678 3955 4404 4678 Size: 219 1151 8 1357 117 277 449 274 Cuts at: 4678 5575 6710 7391 7582 826 Size: 897 1135 681 191 1471 Fragments arranged by size: 1471 1357 1151 1135 897 681 449 277 274 219 191 117 8 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8227 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8227 SmaI CCC'GGG Cuts at: 1872 5554 1872 Size: 3682 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2678 6331 7232 7494 820 Size: 197 926 735 3653 901 262 1553 Fragments arranged by size: 3653 1553 926 901 735 262 197 SnaBI TAC'GTA Cuts at: 493 6018 493 Size: 5525 2702 SpeI A'CTAG_T Cuts at: 152 4697 152 Size: 4545 3682 SphI G_CATG'C Cuts at: 1132 2082 2225 2722 4532 5469 5585 1132 Size: 950 143 497 1810 937 116 3774 Fragments arranged by size: 3774 1810 950 937 497 143 116 SspI AAT'ATT Cuts at: 5 52 5127 6151 5 Size: 47 5075 1024 2081 Fragments arranged by size: 5075 2081 1024 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8227 StyI C'CwwG_G Cuts at: 513 1065 4114 5226 5293 5666 513 Size: 552 3049 1112 67 373 3074 Fragments arranged by size: 3074 3049 1112 552 373 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6254 6271 6424 6609 7054 325 Size: 1255 4674 17 153 185 445 1498 Fragments arranged by size: 4674 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2702 5088 6473 Size: 274 80 33 51 1443 725 2386 1385 Cuts at: 6473 8185 96 Size: 1712 138 Fragments arranged by size: 2386 1712 1443 1385 725 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8187 8219 8187 Size: 32 8195 Tth111I GACn'n_nGTC Cuts at: 1316 4821 1316 Size: 3505 4722 XbaI T'CTAG_A Cuts at: 5563 5563 Size: 8227 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3985 3985 Size: 8227 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8227 XmaI C'CCGG_G Cuts at: 1870 5552 1870 Size: 3682 4545 XmnI GAAnn'nnTTC Cuts at: 2421 2580 4956 6356 2421 Size: 159 2376 1400 4292 Fragments arranged by size: 4292 2376 1400 159 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 6035 276 Size: 53 83 186 936 2245 2256 2468 Fragments arranged by size: 2468 2256 2245 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI