(Circular) (Six-base) MAPSORT of: pFN21AB3626.seq Check: 79 from: 1 to: 8167 pFN21AB3626 8167 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3452 3454 4705 3452 Size: 2 1251 6914 Fragments arranged by size: 6914 1251 2 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5196 5977 278 Size: 53 83 186 936 3660 781 2468 Fragments arranged by size: 3660 2468 936 781 186 83 53 AccI GT'mk_AC Cuts at: 1937 3705 5510 1937 Size: 1768 1805 4594 Fragments arranged by size: 4594 1805 1768 Acc65I G'GTAC_C Cuts at: 5488 5488 Size: 8167 AclI AA'CG_TT Cuts at: 1563 2931 6294 6667 1563 Size: 1368 3363 373 3063 Fragments arranged by size: 3363 3063 1368 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2822 3047 3206 3861 4614 4754 6230 7614 Size: 1190 225 159 655 753 140 1476 1384 Cuts at: 7614 7882 1632 Size: 268 1917 Fragments arranged by size: 1917 1476 1384 1190 753 655 268 225 159 140 AfeI AGC'GCT Cuts at: 4622 4622 Size: 8167 AflII C'TTAA_G Cuts at: 820 1017 4789 4895 820 Size: 197 3772 106 4092 Fragments arranged by size: 4092 3772 197 106 AflIII A'CryG_T Cuts at: 1203 7066 8043 1203 Size: 5863 977 1327 Fragments arranged by size: 5863 1327 977 AgeI A'CCGG_T Cuts at: 6998 6998 Size: 8167 AhdI GACnn_n'nnGTC Cuts at: 2305 5197 6896 2305 Size: 2892 1699 3576 Fragments arranged by size: 3576 2892 1699 AleI CACnn'nnGTG Cuts at: 1166 3281 1166 Size: 2115 6052 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2221 1184 Size: 1037 7130 AlwNI CAG_nnn'CTG Cuts at: 3595 4592 5910 7482 3595 Size: 997 1318 1572 4280 Fragments arranged by size: 4280 1572 1318 997 ApaLI G'TGCA_C Cuts at: 6224 7380 6224 Size: 1156 7011 ApoI r'AATT_y Cuts at: 1493 2753 4076 4874 5174 5402 5476 5702 5854 Size: 1260 1323 798 300 228 74 226 152 Cuts at: 5854 7963 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1323 1260 798 300 228 226 152 74 AseI AT'TA_AT Cuts at: 160 1032 4539 6721 160 Size: 872 3507 2182 1606 Fragments arranged by size: 3507 2182 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8167 AvaI C'yCGr_G Cuts at: 1870 1943 3648 5492 1870 Size: 73 1705 1844 4545 Fragments arranged by size: 4545 1844 1705 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2456 2489 2456 Size: 33 8134 BamHI G'GATC_C Cuts at: 3735 4635 5497 5864 3735 Size: 900 862 367 6038 Fragments arranged by size: 6038 900 862 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3473 5488 6943 618 Size: 325 217 627 1686 2015 1455 1842 Fragments arranged by size: 2015 1842 1686 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2069 2145 4677 5486 1391 Size: 678 76 2532 809 4072 Fragments arranged by size: 4072 2532 809 678 76 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2081 4848 928 Size: 971 182 2767 4247 Fragments arranged by size: 4247 2767 971 182 BbvCI CC'TCA_GC Cuts at: 3398 3398 Size: 8167 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6358 6392 1903 Size: 34 50 34 4337 34 3678 Fragments arranged by size: 4337 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6059 7269 6059 Size: 1210 6957 BclI T'GATC_A Cuts at: 1548 1548 Size: 8167 BfrBI ATG'CAT Cuts at: 4260 4260 Size: 8167 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2149 6778 136 Size: 107 122 71 631 605 477 4629 1525 Fragments arranged by size: 4629 1525 631 605 477 122 107 71 BglII A'GATC_T Cuts at: 4785 8162 4785 Size: 3377 4790 BlpI GC'TnA_GC Cuts at: 5584 5584 Size: 8167 Bme1580I G_kGCm'C Cuts at: 1790 3478 4108 5354 6228 7384 1790 Size: 1688 630 1246 874 1156 2573 Fragments arranged by size: 2573 1688 1246 1156 874 630 BmgBI CAC'GTC Cuts at: 1318 3283 3985 1318 Size: 1965 702 5500 Fragments arranged by size: 5500 1965 702 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2967 5898 6856 Size: 416 53 83 399 24 1550 2931 958 Cuts at: 6856 442 Size: 1753 Fragments arranged by size: 2931 1753 1550 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8167 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 2489 5136 6827 1239 Size: 872 378 2647 1691 2579 Fragments arranged by size: 2647 2579 1691 872 378 Bpu10I CC'TnA_GC Cuts at: 2100 2484 3398 4109 2100 Size: 384 914 711 6158 Fragments arranged by size: 6158 914 711 384 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3566 3725 6292 7157 7455 3566 Size: 159 2567 865 298 4278 Fragments arranged by size: 4278 2567 865 298 159 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2520 3558 3943 6830 882 Size: 617 1021 1038 385 2887 2219 Fragments arranged by size: 2887 2219 1038 1021 617 385 BsaAI yAC'GTr Cuts at: 493 1204 3394 5958 493 Size: 711 2190 2564 2702 Fragments arranged by size: 2702 2564 2190 711 BsaBI GATnn'nnATC Cuts at: 3261 8161 3261 Size: 4900 3267 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2386 3969 5193 5974 Size: 53 83 186 936 853 1583 1224 781 Cuts at: 5974 6356 6991 275 Size: 382 635 1451 Fragments arranged by size: 1583 1451 1224 936 853 781 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2325 4044 6600 6998 7272 7419 7830 2325 Size: 1719 2556 398 274 147 411 2662 Fragments arranged by size: 2662 2556 1719 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2462 2492 3197 3227 3374 Size: 30 165 30 1059 30 705 30 147 Cuts at: 3374 3404 1178 Size: 30 5941 Fragments arranged by size: 5941 1059 705 165 147 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2077 2208 3080 3218 3395 4154 1186 Size: 891 131 872 138 177 759 5199 Fragments arranged by size: 5199 891 872 759 177 138 131 BseYI C'CCAG_C Cuts at: 2152 3554 3881 5397 7370 7866 2152 Size: 1402 327 1516 1973 496 2453 Fragments arranged by size: 2453 1973 1516 1402 496 327 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2181 3259 3394 3488 3515 3530 3776 Size: 573 445 1078 135 94 27 15 246 Cuts at: 3776 4453 4819 1163 Size: 677 366 4511 Fragments arranged by size: 4511 1078 677 573 445 366 246 135 94 27 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3733 5617 6378 7406 1540 Size: 275 186 1732 1884 761 1028 2301 Fragments arranged by size: 2301 1884 1732 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2069 3768 4677 6228 6313 7384 2069 Size: 1699 909 1551 85 1071 2852 Fragments arranged by size: 2852 1699 1551 1071 909 85 BsmI GAATG_Cn' Cuts at: 4262 5678 5771 4262 Size: 1416 93 6658 Fragments arranged by size: 6658 1416 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2145 3478 3768 4108 4677 5354 Size: 399 279 76 1333 290 340 569 677 Cuts at: 5354 5486 6228 6313 7384 1391 Size: 132 742 85 1071 2174 Fragments arranged by size: 2174 1333 1071 742 677 569 399 340 290 279 132 85 76 BspEI T'CCGG_A Cuts at: 7830 7830 Size: 8167 BspHI T'CATG_A Cuts at: 3432 3570 4473 4527 4804 6056 7786 3432 Size: 138 903 54 277 1252 1730 3813 Fragments arranged by size: 3813 1730 1252 903 277 138 54 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3452 3454 3672 4671 4705 5522 844 Size: 2608 2 218 999 34 817 3489 Fragments arranged by size: 3489 2608 999 817 218 34 2 BsrBI CCG'CTC Cuts at: 1394 1823 6054 1394 Size: 429 4231 3507 Fragments arranged by size: 4231 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2041 6662 6836 65 Size: 1183 793 4621 174 1396 Fragments arranged by size: 4621 1396 1183 793 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4428 6811 6998 1700 Size: 110 2618 2383 187 2869 Fragments arranged by size: 2869 2618 2383 187 110 BsrGI T'GTAC_A Cuts at: 96 3338 96 Size: 3242 4925 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8167 BssSI C'ACGA_G Cuts at: 3200 3781 6227 7239 3200 Size: 581 2446 1012 4128 Fragments arranged by size: 4128 2446 1012 581 BstAPI GCAn_nnn'nTGC Cuts at: 794 3595 5224 794 Size: 2801 1629 3737 Fragments arranged by size: 3737 2801 1629 BstBI TT'CG_AA Cuts at: 2915 6971 2915 Size: 4056 4111 BstEII G'GTnAC_C Cuts at: 1184 4751 1184 Size: 3567 4600 BstXI CCAn_nnnn'nTGG Cuts at: 2467 3667 4096 2467 Size: 1200 429 6538 Fragments arranged by size: 6538 1200 429 BstYI r'GATC_y Cuts at: 1972 3078 3591 3735 3748 4030 4377 4635 4785 Size: 1106 513 144 13 282 347 258 150 Cuts at: 4785 5497 5864 6249 6266 7718 7804 7816 8162 Size: 712 367 385 17 1452 86 12 346 Cuts at: 8162 1972 Size: 1977 Fragments arranged by size: 1977 1452 1106 712 513 385 367 347 346 282 258 150 144 86 17 13 12 BtgI C'CryG_G Cuts at: 513 1065 2040 4070 4138 4274 5256 5290 513 Size: 552 975 2030 68 136 982 34 3390 Fragments arranged by size: 3390 2030 982 975 552 136 68 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4068 487 Size: 683 56 2842 4586 Fragments arranged by size: 4586 2842 683 56 BtsI GCAGTG_nn' Cuts at: 2164 5683 6477 6497 2164 Size: 3519 794 20 3834 Fragments arranged by size: 3834 3519 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8167 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4578 4594 4613 4629 529 Size: 35 4014 16 19 16 4067 Fragments arranged by size: 4067 4014 35 19 16 16 DraI TTT'AAA Cuts at: 5463 5471 5463 Size: 8 8159 DraIII CAC_nnn'GTG Cuts at: 3955 5053 3955 Size: 1098 7069 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7174 809 Size: 507 5858 1802 Fragments arranged by size: 5858 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2589 4098 5293 Size: 54 1425 50 275 18 759 1509 1195 Cuts at: 5293 5614 6503 8132 8 Size: 321 889 1629 43 Fragments arranged by size: 1629 1509 1425 1195 889 759 321 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5614 1537 Size: 275 3802 4090 Fragments arranged by size: 4090 3802 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3081 3199 3837 3999 4040 4545 6097 1353 Size: 1728 118 638 162 41 505 1552 3423 Fragments arranged by size: 3423 1728 1552 638 505 162 118 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6754 7128 7274 387 Size: 1064 138 5165 374 146 1280 Fragments arranged by size: 5165 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2067 4675 2067 Size: 2608 5559 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2489 2822 3047 3206 3861 4614 Size: 393 479 378 333 225 159 655 753 Cuts at: 4614 4754 5136 6230 6827 7614 7882 1239 Size: 140 382 1094 597 787 268 1524 Fragments arranged by size: 1524 1094 787 753 655 597 479 393 382 378 333 268 225 159 140 EcoNI CCTnn'n_nnAGG Cuts at: 2103 3743 2103 Size: 1640 6527 EcoO109I rG'GnC_Cy Cuts at: 2095 3804 4022 2095 Size: 1709 218 6240 Fragments arranged by size: 6240 1709 218 EcoRI G'AATT_C Cuts at: 5402 5476 5854 5402 Size: 74 378 7715 Fragments arranged by size: 7715 378 74 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4354 4354 Size: 8167 FspI TGC'GCA Cuts at: 3599 6673 3599 Size: 3074 5093 HaeII r_GCGC'y Cuts at: 1678 4624 7314 1678 Size: 2946 2690 2531 Fragments arranged by size: 2946 2690 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2110 2142 2291 2323 2447 Size: 32 135 32 538 32 149 32 124 Cuts at: 2447 2479 2947 2979 3197 3229 3715 3747 5183 Size: 32 468 32 218 32 486 32 1436 Cuts at: 5183 5215 6808 6840 6882 6914 1373 Size: 32 1593 32 42 32 2626 Fragments arranged by size: 2626 1593 1436 538 486 468 218 149 135 124 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5511 5757 669 Size: 988 281 3573 246 3079 Fragments arranged by size: 3573 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 4544 5425 748 Size: 3796 881 3490 Fragments arranged by size: 3796 3490 881 HpaI GTT'AAC Cuts at: 5757 5757 Size: 8167 KpnI G_GTAC'C Cuts at: 5492 5492 Size: 8167 MfeI C'AATT_G Cuts at: 2347 5085 5766 2347 Size: 2738 681 4748 Fragments arranged by size: 4748 2738 681 MluI A'CGCG_T Cuts at: 8043 8043 Size: 8167 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2112 3684 5431 7281 7465 1305 Size: 807 1572 1747 1850 184 2007 Fragments arranged by size: 2007 1850 1747 1572 807 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2591 4100 5295 10 Size: 54 1425 343 759 1509 1195 2882 Fragments arranged by size: 2882 1509 1425 1195 759 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2203 2714 2847 2916 3281 3431 4143 Size: 648 1037 511 133 69 365 150 712 Cuts at: 4143 4238 4450 6125 6484 6643 518 Size: 95 212 1675 359 159 2042 Fragments arranged by size: 2042 1675 1037 712 648 511 365 359 212 159 150 133 95 69 MspA1I CmG'CkG Cuts at: 1606 3721 4096 4482 5583 6260 7408 7653 1606 Size: 2115 375 386 1101 677 1148 245 2120 Fragments arranged by size: 2120 2115 1148 1101 677 386 375 245 NcoI C'CATG_G Cuts at: 513 1065 2040 4070 4138 4274 5256 5290 513 Size: 552 975 2030 68 136 982 34 3390 Fragments arranged by size: 3390 2030 982 975 552 136 68 34 NdeI CA'TA_TG Cuts at: 387 387 Size: 8167 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8167 NotI GC'GGCC_GC Cuts at: 5614 5614 Size: 8167 NsiI A_TGCA'T Cuts at: 4262 4262 Size: 8167 NspI r_CATG'y Cuts at: 1132 1229 2208 5525 7070 1132 Size: 97 979 3317 1545 2229 Fragments arranged by size: 3317 2229 1545 979 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8167 PciI A'CATG_T Cuts at: 7066 7066 Size: 8167 PflMI CCAn_nnn'nTGG Cuts at: 1108 2265 4071 4353 5291 1108 Size: 1157 1806 282 938 3984 Fragments arranged by size: 3984 1806 1157 938 282 PfoI T'CCnGG_A Cuts at: 3081 3081 Size: 8167 PmeI GTTT'AAAC Cuts at: 5471 5471 Size: 8167 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3174 3206 6236 6268 7749 7781 3174 Size: 32 3030 32 1481 32 3560 Fragments arranged by size: 3560 3030 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3804 4022 3804 Size: 218 7949 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8167 PsiI TTA'TAA Cuts at: 5737 5737 Size: 8167 PstI C_TGCA'G Cuts at: 830 4682 5519 830 Size: 3852 837 3478 Fragments arranged by size: 3852 3478 837 PvuI CG_AT'CG Cuts at: 2001 3733 2001 Size: 1732 6435 PvuII CAG'CTG Cuts at: 3721 4096 3721 Size: 375 7792 RsrII CG'GwC_CG Cuts at: 3541 3541 Size: 8167 SacI G_AGCT'C Cuts at: 2069 4677 2069 Size: 2608 5559 SalI G'TCGA_C Cuts at: 1936 5509 1936 Size: 3573 4594 SanDI GG'GwC_CC Cuts at: 3804 3804 Size: 8167 SapI GCTCTTCn'nnn_ Cuts at: 4545 4545 Size: 8167 SbfI CC_TGCA'GG Cuts at: 5519 5519 Size: 8167 ScaI AGT'ACT Cuts at: 6415 8127 6415 Size: 1712 6455 SexAI A'CCwGG_T Cuts at: 4131 4131 Size: 8167 SfcI C'TryA_G Cuts at: 826 1045 3523 4678 5515 6650 7331 7522 826 Size: 219 2478 1155 837 1135 681 191 1471 Fragments arranged by size: 2478 1471 1155 1135 837 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8167 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8167 SmaI CCC'GGG Cuts at: 1872 5494 1872 Size: 3622 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3581 3740 4789 4895 6271 7172 Size: 197 926 1638 159 1049 106 1376 901 Cuts at: 7172 7434 820 Size: 262 1553 Fragments arranged by size: 1638 1553 1376 1049 926 901 262 197 159 106 SnaBI TAC'GTA Cuts at: 493 5958 493 Size: 5465 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8167 SphI G_CATG'C Cuts at: 1132 2208 5525 1132 Size: 1076 3317 3774 Fragments arranged by size: 3774 3317 1076 SspI AAT'ATT Cuts at: 5 52 2859 6091 5 Size: 47 2807 3232 2081 Fragments arranged by size: 3232 2807 2081 47 StuI AGG'CCT Cuts at: 1517 3209 4510 1517 Size: 1692 1301 5174 Fragments arranged by size: 5174 1692 1301 StyI C'CwwG_G Cuts at: 513 1065 2040 2640 2823 3769 4070 4138 4274 Size: 552 975 600 183 946 301 68 136 Cuts at: 4274 5256 5290 5606 513 Size: 982 34 316 3074 Fragments arranged by size: 3074 982 975 946 600 552 316 301 183 136 68 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2552 6194 6211 6364 6549 6994 325 Size: 1255 972 3642 17 153 185 445 1498 Fragments arranged by size: 3642 1498 1255 972 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2166 3338 4151 Size: 274 80 33 51 1443 189 1172 813 Cuts at: 4151 4821 6413 8125 96 Size: 670 1592 1712 138 Fragments arranged by size: 1712 1592 1443 1172 813 670 274 189 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8127 8159 8127 Size: 32 8135 Tth111I GACn'n_nGTC Cuts at: 1316 4133 1316 Size: 2817 5350 XbaI T'CTAG_A Cuts at: 5503 5503 Size: 8167 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3667 5057 3667 Size: 1390 6777 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8167 XmaI C'CCGG_G Cuts at: 1870 5492 1870 Size: 3622 4545 XmnI GAAnn'nnTTC Cuts at: 3485 4905 6296 3485 Size: 1420 1391 5356 Fragments arranged by size: 5356 1420 1391 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5194 5975 276 Size: 53 83 186 936 3660 781 2468 Fragments arranged by size: 3660 2468 936 781 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI SacII SfiI SfoI SrfI SwaI