Restriction map for
pFN21AB3523
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AviII AvrII BamHI BbrPI
BciVI BclI BfrI BlnI BsaAI
BsaBI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI KspI
MamI MfeI MluI MunI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 290 CGCG CG^CG
AcsI 1279 GAATTC [GA]^AATT[CT]
AhdI 2372 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2229 AGCGCT AGC^GCT
ApoI 1279 GAATTC [GA]^AATT[CT]
AspEI 2372 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 230 GGCGCC GGCGC^C
BbvCI 2053 GCTGAGG CCTCAGC(-5/-2)
BcgI 2292 CGACCTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 2129 CTAG C^TAG
BglII 1079 AGATCT A^GATCT
BsiEI 467 CGGCCG CG[GA][CT]^CG
BsmI 1089 GAATGC GAATGC(1/-1)
BstAPI 489 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 438 GGTGACC G^GT[ACGT]ACC
BstPI 438 GGTGACC G^GT[ACGT]ACC
BstUI 290 CGCG CG^CG
DraIII 694 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1699 GACTACCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 467 CGGCCG C^GGCCG
Eam1105I 2372 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 467 CGGCCG C^GGCCG
Eco47III 2229 AGCGCT AGC^GCT
Eco52I 467 CGGCCG C^GGCCG
EcoO65I 438 GGTGACC G^GT[ACGT]ACC
EcoRI 1279 GAATTC G^AATTC
HincII 1447 GTTGAC GT[CT]^[GA]AC
HindII 1447 GTTGAC GT[CT]^[GA]AC
KasI 230 GGCGCC G^GCGCC
MaeI 2129 CTAG C^TAG
MseI 1490 TTAA T^TAA
MvnI 290 CGCG CG^CG
NarI 230 GGCGCC GG^CGCC
NdeI 779 CATATG CA^TATG
SapI 1029 GCTCTTC GCTCTTC(1/4)
SbfI 1647 CCTGCAGG CCTGCA^GG
SexAI 1703 ACCTGGT A^CC[AT]GGT
SfoI 230 GGCGCC GGC^GCC
SphI 534 GCATGC GCATG^C
Sse8387I 1647 CCTGCAGG CCTGCA^GG
Tru9I 1490 TTAA T^TAA
XcmI 2189 CCATTGTCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 44 enzymes) #
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AccI 205 GTCTAC GT^[AC][GT]AC
2029 GTCTAC
AccIII 983 TCCGGA T^CCGGA
1869 TCCGGA
AcyI 230 GGCGCC G[GA]^CG[CT]C
2011 GACGCC
AflIII 143 ACACGT A^C[GA][CT]GT
770 ACGTGT
AlwNI 257 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
1661 CAGTAGCTG
AocI 55 CCTAAGG CC^T[ACGT]AGG
2170 CCTGAGG
ApaI 1571 GGGCCC GGGCC^C
1610 GGGCCC
Asp700I 1077 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1561 GAACTGGTTC
AvaI 395 CCCGGG C^[CT]CG[GA]G
2210 CCCGGG
BlpI 817 GCTGAGC GC^T[ACGT]AGC
2333 GCTCAGC
Bpu1102I 817 GCTGAGC GC^T[ACGT]AGC
2333 GCTCAGC
BsaHI 230 GGCGCC G[GA]^CG[CT]C
2011 GACGCC
BsaI 65 GAGACC GGTCTC(1/5)
266 GAGACC
BseAI 983 TCCGGA T^CCGGA
1869 TCCGGA
BsgI 446 GTGCAG GTGCAG(16/14)
496 GTGCAG
BsoBI 395 CCCGGG C^[CT]CG[GA]G
2210 CCCGGG
BspEI 983 TCCGGA T^CCGGA
1869 TCCGGA
BspMI 522 GCAGGT ACCTGC(4/8)
1620 GCAGGT
BsrBI 2331 CCGCTC CCGCTC(-3/-3)
1198 GAGCGG
BsrDI 1233 CATTGC GCAATG(2/0)
1848 CATTGC
BsrFI 193 ACCGGC [GA]^CCGG[CT]
1292 ACCGGC
BstYI 1079 AGATCT [GA]^GATC[CT]
2273 GGATCT
Bsu36I 55 CCTAAGG CC^T[ACGT]AGG
2170 CCTGAGG
CelII 817 GCTGAGC GC^T[ACGT]AGC
2333 GCTCAGC
Cfr10I 193 ACCGGC [GA]^CCGG[CT]
1292 ACCGGC
EarI 1030 CTCTTC CTCTTC(1/4)
1537 GAAGAG
Eco81I 55 CCTAAGG CC^T[ACGT]AGG
2170 CCTGAGG
EcoNI 250 CCTGCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
324 CCTTAGCGAGG
HgaI 662 GACGC GACGC(5/10)
2011 GACGC
Hin1I 230 GGCGCC G[GA]^CG[CT]C
2011 GACGCC
Ksp632I 1030 CTCTTC CTCTTC(1/4)
1537 GAAGAG
MflI 1079 AGATCT [GA]^GATC[CT]
2273 GGATCT
MroI 983 TCCGGA T^CCGGA
1869 TCCGGA
NspI 534 GCATGC [GA]CATG^[CT]
845 ACATGC
PstI 1648 CTGCAG CTGCA^G
1819 CTGCAG
SacI 1356 GAGCTC GAGCT^C
1786 GAGCTC
SfiI 1602 GGCCTACGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1644 GGCCCTGCAGGCC
SmaI 395 CCCGGG CCC^GGG
2210 CCCGGG
SmlI 547 CTCAAG C^T[CT][GA]AG
949 CTCAAG
StuI 1601 AGGCCT AGG^CCT
2219 AGGCCT
XhoII 1079 AGATCT [GA]^GATC[CT]
2273 GGATCT
XmaCI 395 CCCGGG C^CCGGG
2210 CCCGGG
XmaI 395 CCCGGG C^CCGGG
2210 CCCGGG
XmnI 1077 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1561 GAACTGGTTC
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AspI AvaII BalI BanI BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflFI PflMI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I