Restriction map for
pFN21AB3523
(Linear) (Six-base) MAPSORT of: KIBB3523.seq Check: 4188 from: 1 to: 2574
KIBB3523.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 513 2574
Size: 513 2061
AccI GT'mk_AC
Cuts at: 0 206 2030 2574
Size: 206 1824 544
Fragments arranged by size:
1824 544 206
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 871 1017 1569 2262 2324 2574
Size: 525 346 146 552 693 62 250
Fragments arranged by size:
693 552 525 346 250 146 62
AfeI AGC'GCT
Cuts at: 0 2231 2574
Size: 2231 343
AflIII A'CryG_T
Cuts at: 0 143 770 2574
Size: 143 627 1804
Fragments arranged by size:
1804 627 143
AhdI GACnn_n'nnGTC
Cuts at: 0 2377 2574
Size: 2377 197
AleI CACnn'nnGTG
Cuts at: 0 1126 2574
Size: 1126 1448
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 862 1319 1663 1834 2574
Size: 862 457 344 171 740
Fragments arranged by size:
862 740 457 344 171
AlwNI CAG_nnn'CTG
Cuts at: 0 262 1666 2574
Size: 262 1404 908
Fragments arranged by size:
1404 908 262
ApaI G_GGCC'C
Cuts at: 0 1575 1614 2574
Size: 1575 39 960
Fragments arranged by size:
1575 960 39
ApoI r'AATT_y
Cuts at: 0 1279 2574
Size: 1279 1295
AvaI C'yCGr_G
Cuts at: 0 395 2210 2574
Size: 395 1815 364
Fragments arranged by size:
1815 395 364
BanI G'GyrC_C
Cuts at: 0 230 745 1203 1578 1746 2431 2574
Size: 230 515 458 375 168 685 143
Fragments arranged by size:
685 515 458 375 230 168 143
BanII G_rGCy'C
Cuts at: 0 1360 1575 1614 1790 1880 2143 2574
Size: 1360 215 39 176 90 263 431
Fragments arranged by size:
1360 431 263 215 176 90 39
BbeI G_GCGC'C
Cuts at: 0 234 2574
Size: 234 2340
BbsI GAAGACnn'nnnn_
Cuts at: 0 868 1916 1922 2574
Size: 868 1048 6 652
Fragments arranged by size:
1048 868 652 6
BbvCI CC'TCA_GC
Cuts at: 0 2054 2574
Size: 2054 520
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2281 2315 2574
Size: 2281 34 259
Fragments arranged by size:
2281 259 34
BglI GCCn_nnn'nGGC
Cuts at: 0 291 1609 1651 2574
Size: 291 1318 42 923
Fragments arranged by size:
1318 923 291 42
BglII A'GATC_T
Cuts at: 0 1079 2574
Size: 1079 1495
BlpI GC'TnA_GC
Cuts at: 0 818 2334 2574
Size: 818 1516 240
Fragments arranged by size:
1516 818 240
Bme1580I G_kGCm'C
Cuts at: 0 748 1475 1575 1583 1614 1751 1813 2434
Size: 748 727 100 8 31 137 62 621
Cuts at: 2434 2574
Size: 140
Fragments arranged by size:
748 727 621 140 137 100 62 31
8
BmgBI CAC'GTC
Cuts at: 0 771 2574
Size: 771 1803
BmrI ACTGGGnnnn_n'
Cuts at: 0 415 1752 1794 2079 2574
Size: 415 1337 42 285 495
Fragments arranged by size:
1337 495 415 285 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2349 2574
Size: 2349 225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 284 406 1093 1528 1618 1656 2574
Size: 83 201 122 687 435 90 38 918
Fragments arranged by size:
918 687 435 201 122 90 83 38
Bpu10I CC'TnA_GC
Cuts at: 0 325 1616 2054 2574
Size: 325 1291 438 520
Fragments arranged by size:
1291 520 438 325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 934 2574
Size: 532 402 1640
Fragments arranged by size:
1640 532 402
BsaI GGTCTCn'nnnn_
Cuts at: 0 59 260 2574
Size: 59 201 2314
Fragments arranged by size:
2314 201 59
BsaHI Gr'CG_yC
Cuts at: 0 231 2012 2574
Size: 231 1781 562
Fragments arranged by size:
1781 562 231
BsaWI w'CCGG_w
Cuts at: 0 983 1179 1869 2574
Size: 983 196 690 705
Fragments arranged by size:
983 705 690 196
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 56 86 463 493 755 785 1903 1933
Size: 56 30 377 30 262 30 1118 30
Cuts at: 1933 2574
Size: 641
Fragments arranged by size:
1118 641 377 262 56 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 256 346 1513 1531 1555 1798 1846
Size: 31 225 90 1167 18 24 243 48
Cuts at: 1846 1849 1932 2574
Size: 3 83 642
Fragments arranged by size:
1167 642 243 225 90 83 48 31
24 18 3
BseYI C'CCAG_C
Cuts at: 0 741 808 1137 1439 2574
Size: 741 67 329 302 1135
Fragments arranged by size:
1135 741 329 302 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 517 2574
Size: 467 50 2057
Fragments arranged by size:
2057 467 50
BsiEI CG_ry'CG
Cuts at: 0 470 2574
Size: 470 2104
BsiHKAI G_wGCw'C
Cuts at: 0 598 707 824 1031 1360 1642 1790 2574
Size: 598 109 117 207 329 282 148 784
Fragments arranged by size:
784 598 329 282 207 148 117 109
BsmI GAATG_Cn'
Cuts at: 0 1095 2574
Size: 1095 1479
Bsp1286I G_dGCh'C
Cuts at: 0 598 707 748 824 1031 1360 1475 1575
Size: 598 109 41 76 207 329 115 100
Cuts at: 1575 1583 1614 1642 1751 1790 1813 1880 2143
Size: 8 31 28 109 39 23 67 263
Cuts at: 2143 2434 2574
Size: 291 140
Fragments arranged by size:
598 329 291 263 207 140 115 109
109 100 76 67 41 39 31 28
23 8
BspEI T'CCGG_A
Cuts at: 0 983 1869 2574
Size: 983 886 705
Fragments arranged by size:
983 886 705
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 513 1611 2574
Size: 513 1098 963
Fragments arranged by size:
1098 963 513
BsrBI CCG'CTC
Cuts at: 0 1200 2333 2574
Size: 1200 1133 241
Fragments arranged by size:
1200 1133 241
BsrDI GCAATG_nn'
Cuts at: 0 1232 1847 2574
Size: 1232 615 727
Fragments arranged by size:
1232 727 615
BsrFI r'CCGG_y
Cuts at: 0 193 1292 2574
Size: 193 1099 1282
Fragments arranged by size:
1282 1099 193
BstAPI GCAn_nnn'nTGC
Cuts at: 0 495 2574
Size: 495 2079
BstEII G'GTnAC_C
Cuts at: 0 438 2574
Size: 438 2136
BstXI CCAn_nnnn'nTGG
Cuts at: 0 294 450 910 1758 2538 2574
Size: 294 156 460 848 780 36
Fragments arranged by size:
848 780 460 294 156 36
BstYI r'GATC_y
Cuts at: 0 1079 2273 2574
Size: 1079 1194 301
Fragments arranged by size:
1194 1079 301
Bsu36I CC'TnA_GG
Cuts at: 0 56 2171 2574
Size: 56 2115 403
Fragments arranged by size:
2115 403 56
BtgI C'CryG_G
Cuts at: 0 1143 1475 2198 2312 2574
Size: 1143 332 723 114 262
Fragments arranged by size:
1143 723 332 262 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1205 1937 2574
Size: 1205 732 637
Fragments arranged by size:
1205 732 637
BtsI GCAGTG_nn'
Cuts at: 0 455 684 1552 2574
Size: 455 229 868 1022
Fragments arranged by size:
1022 868 455 229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1665 1700 2574
Size: 1665 35 874
Fragments arranged by size:
1665 874 35
DraIII CAC_nnn'GTG
Cuts at: 0 699 2574
Size: 699 1875
DrdI GACnn_nn'nnGTC
Cuts at: 0 1705 2574
Size: 1705 869
EaeI y'GGCC_r
Cuts at: 0 195 467 1130 1415 2567 2574
Size: 195 272 663 285 1152 7
Fragments arranged by size:
1152 663 285 272 195 7
EagI C'GGCC_G
Cuts at: 0 467 2574
Size: 467 2107
EarI CTCTTCn'nnn_
Cuts at: 0 1036 1532 2574
Size: 1036 496 1042
Fragments arranged by size:
1042 1036 496
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 237 329 2574
Size: 19 218 92 2245
Fragments arranged by size:
2245 218 92 19
EcoICRI GAG'CTC
Cuts at: 0 1358 1788 2574
Size: 1358 430 786
Fragments arranged by size:
1358 786 430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 284 406 525 871 1017 1093 1528
Size: 83 201 122 119 346 146 76 435
Cuts at: 1528 1569 1618 1656 2262 2324 2574
Size: 41 49 38 606 62 250
Fragments arranged by size:
606 435 346 250 201 146 122 119
83 76 62 49 41 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 254 328 2574
Size: 254 74 2246
Fragments arranged by size:
2246 254 74
EcoO109I rG'GnC_Cy
Cuts at: 0 51 123 246 456 1254 1610 1611 2371
Size: 51 72 123 210 798 356 1 760
Cuts at: 2371 2535 2574
Size: 164 39
Fragments arranged by size:
798 760 356 210 164 123 72 51
39 1
EcoRI G'AATT_C
Cuts at: 0 1279 2574
Size: 1279 1295
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 555 1519 1746 1906 2574
Size: 555 964 227 160 668
Fragments arranged by size:
964 668 555 227 160
HaeII r_GCGC'y
Cuts at: 0 25 234 2233 2574
Size: 25 209 1999 341
Fragments arranged by size:
1999 341 209 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 56 88 463 495 760 792 811 843
Size: 56 32 375 32 265 32 19 32
Cuts at: 843 1903 1935 2265 2297 2335 2363 2367 2395
Size: 1060 32 330 32 38 28 4 28
Cuts at: 2395 2474 2506 2574
Size: 79 32 68
Fragments arranged by size:
1060 375 330 265 79 68 56 38
32 32 32 32 32 32 32 28
28 19 4
HincII GTy'rAC
Cuts at: 0 1449 2574
Size: 1449 1125
KasI G'GCGC_C
Cuts at: 0 230 2574
Size: 230 2344
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 866 1972 2574
Size: 536 330 1106 602
Fragments arranged by size:
1106 602 536 330
MscI TGG'CCA
Cuts at: 0 1132 1417 2569 2574
Size: 1132 285 1152 5
Fragments arranged by size:
1152 1132 285 5
MslI CAynn'nnrTG
Cuts at: 0 136 844 1126 1774 2087 2574
Size: 136 708 282 648 313 487
Fragments arranged by size:
708 648 487 313 282 136
MspA1I CmG'CkG
Cuts at: 0 906 1001 1464 1986 2048 2338 2574
Size: 906 95 463 522 62 290 236
Fragments arranged by size:
906 522 463 290 236 95 62
NarI GG'CG_CC
Cuts at: 0 231 2574
Size: 231 2343
NcoI C'CATG_G
Cuts at: 0 1143 1475 2198 2312 2574
Size: 1143 332 723 114 262
Fragments arranged by size:
1143 723 332 262 114
NdeI CA'TA_TG
Cuts at: 0 780 2574
Size: 780 1794
NspI r_CATG'y
Cuts at: 0 538 849 2574
Size: 538 311 1725
Fragments arranged by size:
1725 538 311
PflMI CCAn_nnn'nTGG
Cuts at: 0 699 1989 2069 2565 2574
Size: 699 1290 80 496 9
Fragments arranged by size:
1290 699 496 80 9
PfoI T'CCnGG_A
Cuts at: 0 800 929 2381 2391 2574
Size: 800 129 1452 10 183
Fragments arranged by size:
1452 800 183 129 10
PpuMI rG'GwC_Cy
Cuts at: 0 51 246 2371 2535 2574
Size: 51 195 2125 164 39
Fragments arranged by size:
2125 195 164 51 39
PspOMI G'GGCC_C
Cuts at: 0 1571 1610 2574
Size: 1571 39 964
Fragments arranged by size:
1571 964 39
PstI C_TGCA'G
Cuts at: 0 1652 1823 2574
Size: 1652 171 751
Fragments arranged by size:
1652 751 171
PvuII CAG'CTG
Cuts at: 0 1001 1464 1986 2338 2574
Size: 1001 463 522 352 236
Fragments arranged by size:
1001 522 463 352 236
SacI G_AGCT'C
Cuts at: 0 1360 1790 2574
Size: 1360 430 784
Fragments arranged by size:
1360 784 430
SanDI GG'GwC_CC
Cuts at: 0 51 2574
Size: 51 2523
SapI GCTCTTCn'nnn_
Cuts at: 0 1036 2574
Size: 1036 1538
SbfI CC_TGCA'GG
Cuts at: 0 1652 2574
Size: 1652 922
SexAI A'CCwGG_T
Cuts at: 0 1703 2574
Size: 1703 871
SfcI C'TryA_G
Cuts at: 0 254 1648 1819 2574
Size: 254 1394 171 755
Fragments arranged by size:
1394 755 254 171
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1609 1651 2574
Size: 1609 42 923
Fragments arranged by size:
1609 923 42
SfoI GGC'GCC
Cuts at: 0 232 2574
Size: 232 2342
SmaI CCC'GGG
Cuts at: 0 397 2212 2574
Size: 397 1815 362
Fragments arranged by size:
1815 397 362
SmlI C'TyrA_G
Cuts at: 0 547 949 2574
Size: 547 402 1625
Fragments arranged by size:
1625 547 402
SphI G_CATG'C
Cuts at: 0 538 2574
Size: 538 2036
StuI AGG'CCT
Cuts at: 0 1603 2221 2574
Size: 1603 618 353
Fragments arranged by size:
1603 618 353
StyI C'CwwG_G
Cuts at: 0 1143 1475 1890 2198 2312 2531 2574
Size: 1143 332 415 308 114 219 43
Fragments arranged by size:
1143 415 332 308 219 114 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1138 1453 1734 2475 2574
Size: 1138 315 281 741 99
Fragments arranged by size:
1138 741 315 281 99
TatI w'GTAC_w
Cuts at: 0 773 1433 2574
Size: 773 660 1141
Fragments arranged by size:
1141 773 660
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 755 787 2574
Size: 755 32 1787
Fragments arranged by size:
1787 755 32
Tth111I GACn'n_nGTC
Cuts at: 0 70 424 796 2574
Size: 70 354 372 1778
Fragments arranged by size:
1778 372 354 70
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2196 2574
Size: 2196 378
XmaI C'CCGG_G
Cuts at: 0 395 2210 2574
Size: 395 1815 364
Fragments arranged by size:
1815 395 364
XmnI GAAnn'nnTTC
Cuts at: 0 1081 1565 2574
Size: 1081 484 1009
Fragments arranged by size:
1081 1009 484
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AlfI
AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NarI NcoI NdeI NspI PflMI PfoI PpuMI
PspOMI PstI PvuII SacI SanDI SapI SbfI SexAI
SfcI SfiI SfoI SmaI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI
BmtI BsaAI BsaBI BsiWI BsmBI BspHI BsrGI BssHII
BssSI BstBI BstZ17I ClaI DraI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI NaeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI ScaI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI XbaI XhoI ZraI