Restriction map for
pFN21AB3493
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BssHII BssSI
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DrdI Eco47III EcoRI EcoRV
EcoT22I FbaI FseI FspI HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspI SwaI Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 493 GAATTT [GA]^AATT[CT]
AhdI 1105 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 603 CCTGAGG CC^T[ACGT]AGG
ApaI 1608 GGGCCC GGGCC^C
ApoI 493 GAATTT [GA]^AATT[CT]
Asp700I 1560 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1105 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1783 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1334 TGGCCA TGG^CCA
BamHI 1529 GGATCC G^GATCC
BanI 1861 GGTGCC G^G[CT][GA]CC
BbsI 348 GAAGAC GAAGAC(2/6)
BglII 1670 AGATCT A^GATCT
BpuAI 348 GAAGAC GAAGAC(2/6)
BsmBI 115 GAGACG CGTCTC(1/5)
BsmI 303 GAATGC GAATGC(1/-1)
Bsp1407I 626 TGTACA T^GTACA
BspMI 389 ACCTGC ACCTGC(4/8)
BsrGI 626 TGTACA T^GTACA
Bsu36I 603 CCTGAGG CC^T[ACGT]AGG
DraI 1242 TTTAAA TTT^AAA
DraII 1607 AGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 75 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1752 CCACGG C^C[GA][CT]GG
Eam1105I 1105 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 603 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1607 AGGGCCC [GA]G^G[ACGT]CC[CT]
HincII 808 GTCAAC GT[CT]^[GA]AC
HindII 808 GTCAAC GT[CT]^[GA]AC
HindIII 1204 AAGCTT A^AGCTT
MluNI 1334 TGGCCA TGG^CCA
MscI 1334 TGGCCA TGG^CCA
NruI 447 TCGCGA TCG^CGA
PflFI 1783 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 184 CAGCTG CAG^CTG
SbfI 228 CCTGCAGG CCTGCA^GG
SfiI 234 GGCCGACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 245 CCCGGG CCC^GGG
Sse8387I 228 CCTGCAGG CCTGCA^GG
SspBI 626 TGTACA T^GTACA
StuI 551 AGGCCT AGG^CCT
Tsp509I 494 AATT ^AATT
Tth111I 1783 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 581 CCAGCGCCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 245 CCCGGG C^CCGGG
XmaI 245 CCCGGG C^CCGGG
XmnI 1560 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 166 GTCTAC GT^[AC][GT]AC
964 GTCTAC
AfaI 627 GTAC GT^AC
1833 GTAC
AvaII 423 GGACC G^G[AT]CC
1788 GGTCC
BglI 235 GCCGACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
307 GCCCAGGTGGC
BsmFI 567 GTCCC GGGAC(10/14)
1186 GTCCC
BstAPI 222 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
681 GCAGGATGTGC
BstEII 69 GGTCACC G^GT[ACGT]ACC
1087 GGTGACC
BstPI 69 GGTCACC G^GT[ACGT]ACC
1087 GGTGACC
EagI 1465 CGGCCG C^GGCCG
1626 CGGCCG
EclXI 1465 CGGCCG C^GGCCG
1626 CGGCCG
Eco52I 1465 CGGCCG C^GGCCG
1626 CGGCCG
EcoO65I 69 GGTCACC G^GT[ACGT]ACC
1087 GGTGACC
EcoT14I 1313 CCAAGG C^C[AT][AT]GG
1715 CCAAGG
HaeII 583 AGCGCC [GA]GCGC^[CT]
1636 AGCGCC
HinfI 837 GATTC G^A[ACGT]TC
947 GAATC
MseI 1243 TTAA T^TAA
1370 TTAA
MspA1I 184 CAGCTG C[AC]G^C[GT]G
430 CCGCTG
RsaI 627 GTAC GT^AC
1833 GTAC
SacI 1069 GAGCTC GAGCT^C
1351 GAGCTC
SmlI 844 CTCAAG C^T[CT][GA]AG
1306 CTCAAG
StyI 1313 CCAAGG C^C[AT][AT]GG
1715 CCAAGG
TfiI 837 GATTC G^A[AT]TC
947 GAATC
Tru9I 1243 TTAA T^TAA
1370 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AspHI AvaI BanII BbvI
BcnI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII EaeI
EarI EcoNI EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PstI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI Tsp45I
TspRI TthHB8I XhoII