(Circular) (Six-base) MAPSORT of: pFN21AB3493.seq Check: 1626 from: 1 to: 6595 pFN21AB3493 6595 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2403 2403 Size: 6595 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4405 278 Size: 53 83 186 936 2869 2468 Fragments arranged by size: 2869 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2172 2970 3938 1937 Size: 235 798 968 4594 Fragments arranged by size: 4594 968 798 235 Acc65I G'GTAC_C Cuts at: 3916 3916 Size: 6595 AclI AA'CG_TT Cuts at: 1563 4722 5095 1563 Size: 3159 373 3063 Fragments arranged by size: 3159 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2357 3068 4658 6042 6310 1632 Size: 725 711 1590 1384 268 1917 Fragments arranged by size: 1917 1590 1384 725 711 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6398 AflIII A'CryG_T Cuts at: 1203 5494 6471 1203 Size: 4291 977 1327 Fragments arranged by size: 4291 1327 977 AgeI A'CCGG_T Cuts at: 5426 5426 Size: 6595 AhdI GACnn_n'nnGTC Cuts at: 3115 5324 3115 Size: 2209 4386 AleI CACnn'nnGTG Cuts at: 1166 3731 1166 Size: 2565 4030 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2032 1184 Size: 848 5747 AlwNI CAG_nnn'CTG Cuts at: 2233 2899 3508 4338 5910 2233 Size: 666 609 830 1572 2918 Fragments arranged by size: 2918 1572 830 666 609 ApaI G_GGCC'C Cuts at: 3617 3617 Size: 6595 ApaLI G'TGCA_C Cuts at: 4652 5808 4652 Size: 1156 5439 ApoI r'AATT_y Cuts at: 1493 2498 3904 4130 4282 6391 1493 Size: 1005 1406 226 152 2109 1697 Fragments arranged by size: 2109 1697 1406 1005 226 152 AseI AT'TA_AT Cuts at: 160 1032 5149 160 Size: 872 4117 1606 Fragments arranged by size: 4117 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6595 AvaI C'yCGr_G Cuts at: 1870 1943 2250 2510 2978 3920 1870 Size: 73 307 260 468 942 4545 Fragments arranged by size: 4545 942 468 307 260 73 BamHI G'GATC_C Cuts at: 3534 3925 4292 3534 Size: 391 367 5837 Fragments arranged by size: 5837 391 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3866 3916 5371 618 Size: 325 217 627 2079 50 1455 1842 Fragments arranged by size: 2079 1842 1455 627 325 217 50 BanII G_rGCy'C Cuts at: 1391 2061 2141 3078 3229 3360 3617 3914 1391 Size: 670 80 937 151 131 257 297 4072 Fragments arranged by size: 4072 937 670 297 257 151 131 80 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2360 928 Size: 971 461 5163 Fragments arranged by size: 5163 971 461 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4786 4820 1903 Size: 34 2849 34 3678 Fragments arranged by size: 3678 2849 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4487 5697 4487 Size: 1210 5385 BclI T'GATC_A Cuts at: 1548 1548 Size: 6595 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2246 2318 5206 Size: 107 122 71 631 605 574 72 2888 Cuts at: 5206 136 Size: 1525 Fragments arranged by size: 2888 1525 631 605 574 122 107 72 71 BglII A'GATC_T Cuts at: 3675 6590 3675 Size: 2915 3680 BlpI GC'TnA_GC Cuts at: 4012 4012 Size: 6595 Bme1580I G_kGCm'C Cuts at: 1790 2021 2156 3617 4656 5812 1790 Size: 231 135 1461 1039 1156 2573 Fragments arranged by size: 2573 1461 1156 1039 231 135 BmgBI CAC'GTC Cuts at: 1318 2124 2358 1318 Size: 806 234 5555 Fragments arranged by size: 5555 806 234 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3078 3553 4326 Size: 416 53 83 399 24 1661 475 773 Cuts at: 4326 5284 442 Size: 958 1753 Fragments arranged by size: 1753 1661 958 773 475 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6595 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2794 2794 Size: 6595 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2618 2948 3053 3120 3347 3383 5255 Size: 836 543 330 105 67 227 36 1872 Cuts at: 5255 1239 Size: 2579 Fragments arranged by size: 2579 1872 836 543 330 227 105 67 36 Bpu10I CC'TnA_GC Cuts at: 2576 3354 2576 Size: 778 5817 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2834 3296 4720 5585 5883 2834 Size: 462 1424 865 298 3546 Fragments arranged by size: 3546 1424 865 462 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2765 3157 3323 5258 882 Size: 617 1266 392 166 1935 2219 Fragments arranged by size: 2219 1935 1266 617 392 166 BsaAI yAC'GTr Cuts at: 493 1204 4386 493 Size: 711 3182 2702 Fragments arranged by size: 3182 2702 711 BsaBI GATnn'nnATC Cuts at: 6589 6589 Size: 6595 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2276 2651 2763 4402 Size: 53 83 186 936 743 375 112 1639 Cuts at: 4402 4784 5419 275 Size: 382 635 1451 Fragments arranged by size: 1639 1451 936 743 635 382 375 186 112 83 53 BsaWI w'CCGG_w Cuts at: 5028 5426 5700 5847 6258 5028 Size: 398 274 147 411 5365 Fragments arranged by size: 5365 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2024 2054 3342 3372 3629 Size: 30 165 30 621 30 1288 30 257 Cuts at: 3629 3659 1178 Size: 30 4114 Fragments arranged by size: 4114 1288 621 257 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2816 2837 2897 1186 Size: 859 771 21 60 4884 Fragments arranged by size: 4884 859 771 60 21 BseYI C'CCAG_C Cuts at: 2300 2478 3112 3203 3508 5798 6294 2300 Size: 178 634 91 305 2290 496 2601 Fragments arranged by size: 2601 2290 634 496 305 178 91 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2177 2280 3500 1163 Size: 573 441 103 1220 4258 Fragments arranged by size: 4258 1220 573 441 103 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2186 3473 3634 4045 4806 5834 Size: 275 186 185 1287 161 411 761 1028 Cuts at: 5834 1540 Size: 2301 Fragments arranged by size: 2301 1287 1028 761 411 275 186 185 161 BsiHKAI G_wGCw'C Cuts at: 2045 2394 2517 2662 3026 3078 3360 4656 4741 Size: 349 123 145 364 52 282 1296 85 Cuts at: 4741 5812 2045 Size: 1071 2828 Fragments arranged by size: 2828 1296 1071 364 349 282 145 123 85 52 BsmI GAATG_Cn' Cuts at: 2314 4106 4199 2314 Size: 1792 93 4710 Fragments arranged by size: 4710 1792 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2114 2114 Size: 6595 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2045 2061 2141 2156 2394 2517 Size: 399 231 24 16 80 15 238 123 Cuts at: 2517 2662 3026 3078 3229 3360 3617 3914 4656 Size: 145 364 52 151 131 257 297 742 Cuts at: 4656 4741 5812 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 364 297 257 238 231 151 145 131 123 85 80 52 24 16 15 BspEI T'CCGG_A Cuts at: 6258 6258 Size: 6595 BspHI T'CATG_A Cuts at: 4484 6214 4484 Size: 1730 4865 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2403 3950 844 Size: 1559 1547 3489 Fragments arranged by size: 3489 1559 1547 BsrBI CCG'CTC Cuts at: 1394 1823 4482 1394 Size: 429 2659 3507 Fragments arranged by size: 3507 2659 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5090 5264 65 Size: 1183 3842 174 1396 Fragments arranged by size: 3842 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2071 3468 3606 5239 5426 1700 Size: 110 261 1397 138 1633 187 2869 Fragments arranged by size: 2869 1633 1397 261 187 138 110 BsrGI T'GTAC_A Cuts at: 96 2631 96 Size: 2535 4060 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6595 BssSI C'ACGA_G Cuts at: 4655 5667 4655 Size: 1012 5583 BstAPI GCAn_nnn'nTGC Cuts at: 794 2233 2692 794 Size: 1439 459 4697 Fragments arranged by size: 4697 1439 459 BstBI TT'CG_AA Cuts at: 5399 5399 Size: 6595 BstEII G'GTnAC_C Cuts at: 1184 2074 3092 1184 Size: 890 1018 4687 Fragments arranged by size: 4687 1018 890 BstXI CCAn_nnnn'nTGG Cuts at: 2053 3325 3733 2053 Size: 1272 408 4915 Fragments arranged by size: 4915 1272 408 BstYI r'GATC_y Cuts at: 1972 2865 3534 3675 3925 4292 4677 4694 6146 Size: 893 669 141 250 367 385 17 1452 Cuts at: 6146 6232 6244 6590 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 893 669 385 367 346 250 141 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2609 2609 Size: 6595 BtgI C'CryG_G Cuts at: 513 1065 2004 3757 513 Size: 552 939 1753 3351 Fragments arranged by size: 3351 1753 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5856 Fragments arranged by size: 5856 683 56 BtsI GCAGTG_nn' Cuts at: 2165 2329 3059 3517 4111 4905 4925 2165 Size: 164 730 458 594 794 20 3835 Fragments arranged by size: 3835 794 730 594 458 164 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6595 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3556 3591 529 Size: 35 2992 35 3533 Fragments arranged by size: 3533 2992 35 35 DraI TTT'AAA Cuts at: 3249 3899 3249 Size: 650 5945 DraIII CAC_nnn'GTG Cuts at: 2085 2085 Size: 6595 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5602 809 Size: 507 4286 1802 Fragments arranged by size: 4286 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2089 3339 Size: 54 1425 50 275 18 195 64 1250 Cuts at: 3339 3470 3631 4042 4931 6560 8 Size: 131 161 411 889 1629 43 Fragments arranged by size: 1629 1425 1250 889 411 275 195 161 131 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3470 3631 4042 1537 Size: 275 1658 161 411 4090 Fragments arranged by size: 4090 1658 411 275 161 EarI CTCTTCn'nnn_ Cuts at: 1353 2793 2801 2852 4525 1353 Size: 1440 8 51 1673 3423 Fragments arranged by size: 3423 1673 1440 51 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5182 5556 5702 387 Size: 1064 138 3593 374 146 1280 Fragments arranged by size: 3593 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3076 3358 3076 Size: 282 6313 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2357 2618 2948 3053 3068 3120 Size: 393 443 282 261 330 105 15 52 Cuts at: 3120 3347 3383 4658 5255 6042 6310 1239 Size: 227 36 1275 597 787 268 1524 Fragments arranged by size: 1524 1275 787 597 443 393 330 282 268 261 227 105 52 36 15 EcoNI CCTnn'n_nnAGG Cuts at: 2027 2339 2579 2782 2027 Size: 312 240 203 5840 Fragments arranged by size: 5840 312 240 203 EcoO109I rG'GnC_Cy Cuts at: 3613 3613 Size: 6595 EcoRI G'AATT_C Cuts at: 3904 4282 3904 Size: 378 6217 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2377 2773 3586 2377 Size: 396 813 5386 Fragments arranged by size: 5386 813 396 FspI TGC'GCA Cuts at: 5101 5101 Size: 6595 HaeII r_GCGC'y Cuts at: 1678 2592 3645 5742 1678 Size: 914 1053 2097 2531 Fragments arranged by size: 2531 2097 1053 914 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 2921 2953 3101 Size: 32 135 32 449 32 868 32 148 Cuts at: 3101 3133 3342 3374 5236 5268 5310 5342 1373 Size: 32 209 32 1862 32 42 32 2626 Fragments arranged by size: 2626 1862 868 449 209 148 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2815 3939 4185 669 Size: 988 281 877 1124 246 3079 Fragments arranged by size: 3079 1124 988 877 281 246 HindIII A'AGCT_T Cuts at: 748 3209 748 Size: 2461 4134 HpaI GTT'AAC Cuts at: 4185 4185 Size: 6595 KpnI G_GTAC'C Cuts at: 3920 3920 Size: 6595 MfeI C'AATT_G Cuts at: 4194 4194 Size: 6595 MluI A'CGCG_T Cuts at: 6471 6471 Size: 6595 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5709 5893 1305 Size: 4404 184 2007 Fragments arranged by size: 4404 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3341 10 Size: 54 1425 343 1509 3264 Fragments arranged by size: 3264 1509 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2149 2744 3731 4553 4912 5071 518 Size: 648 983 595 987 822 359 159 2042 Fragments arranged by size: 2042 987 983 822 648 595 359 159 MspA1I CmG'CkG Cuts at: 1606 2191 2437 4011 4688 5836 6081 1606 Size: 585 246 1574 677 1148 245 2120 Fragments arranged by size: 2120 1574 1148 677 585 246 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5104 Fragments arranged by size: 5104 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6595 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6595 NotI GC'GGCC_GC Cuts at: 4042 4042 Size: 6595 NruI TCG'CGA Cuts at: 2454 2454 Size: 6595 NspI r_CATG'y Cuts at: 1132 1229 3953 5498 1132 Size: 97 2724 1545 2229 Fragments arranged by size: 2724 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6595 PciI A'CATG_T Cuts at: 5494 5494 Size: 6595 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6595 PfoI T'CCnGG_A Cuts at: 2424 3069 3360 3384 2424 Size: 645 291 24 5635 Fragments arranged by size: 5635 645 291 24 PmeI GTTT'AAAC Cuts at: 3899 3899 Size: 6595 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4664 4696 6177 6209 4664 Size: 32 1481 32 5050 Fragments arranged by size: 5050 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6595 PsiI TTA'TAA Cuts at: 4165 4165 Size: 6595 PspOMI G'GGCC_C Cuts at: 3613 3613 Size: 6595 PstI C_TGCA'G Cuts at: 830 2238 2400 2442 3274 3561 3947 830 Size: 1408 162 42 832 287 386 3478 Fragments arranged by size: 3478 1408 832 386 287 162 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6595 PvuII CAG'CTG Cuts at: 2191 2191 Size: 6595 SacI G_AGCT'C Cuts at: 3078 3360 3078 Size: 282 6313 SalI G'TCGA_C Cuts at: 1936 3937 1936 Size: 2001 4594 SbfI CC_TGCA'GG Cuts at: 2238 3947 2238 Size: 1709 4886 ScaI AGT'ACT Cuts at: 4843 6555 4843 Size: 1712 4883 SfcI C'TryA_G Cuts at: 826 1045 2234 2396 2438 3270 3557 3943 5078 Size: 219 1189 162 42 832 287 386 1135 Cuts at: 5078 5759 5950 826 Size: 681 191 1471 Fragments arranged by size: 1471 1189 1135 832 681 386 287 219 191 162 42 SfiI GGCCn_nnn'nGGCC Cuts at: 2246 2246 Size: 6595 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6595 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6595 SmaI CCC'GGG Cuts at: 1872 2252 3922 1872 Size: 380 1670 4545 Fragments arranged by size: 4545 1670 380 SmlI C'TyrA_G Cuts at: 820 1017 1943 2849 3311 4699 5600 5862 820 Size: 197 926 906 462 1388 901 262 1553 Fragments arranged by size: 1553 1388 926 906 901 462 262 197 SnaBI TAC'GTA Cuts at: 493 4386 493 Size: 3893 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6595 SphI G_CATG'C Cuts at: 1132 3953 1132 Size: 2821 3774 SspI AAT'ATT Cuts at: 5 52 4519 5 Size: 47 4467 2081 Fragments arranged by size: 4467 2081 47 StuI AGG'CCT Cuts at: 1517 2558 1517 Size: 1041 5554 StyI C'CwwG_G Cuts at: 513 1065 2004 3318 3720 3891 4034 513 Size: 552 939 1314 402 171 143 3074 Fragments arranged by size: 3074 1314 939 552 402 171 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2314 4622 4639 4792 4977 5422 325 Size: 1255 734 2308 17 153 185 445 1498 Fragments arranged by size: 2308 1498 1255 734 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2631 4841 6553 Size: 274 80 33 51 1443 654 2210 1712 Cuts at: 6553 96 Size: 138 Fragments arranged by size: 2210 1712 1443 654 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2024 2056 6555 6587 2024 Size: 32 4499 32 2032 Fragments arranged by size: 4499 2032 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3791 1316 Size: 2475 4120 XbaI T'CTAG_A Cuts at: 3931 3931 Size: 6595 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2593 2593 Size: 6595 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6595 XmaI C'CCGG_G Cuts at: 1870 2250 3920 1870 Size: 380 1670 4545 Fragments arranged by size: 4545 1670 380 XmnI GAAnn'nnTTC Cuts at: 3569 4724 3569 Size: 1155 5440 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4403 276 Size: 53 83 186 936 2869 2468 Fragments arranged by size: 2869 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI