(Circular) (Six-base) MAPSORT of: pFN21AB3481.seq Check: 8427 from: 1 to: 7051 pFN21AB3481 7051 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2128 2128 Size: 7051 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4861 278 Size: 53 83 186 936 3325 2468 Fragments arranged by size: 3325 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4394 1937 Size: 2457 4594 Acc65I G'GTAC_C Cuts at: 4372 4372 Size: 7051 AclI AA'CG_TT Cuts at: 1563 2851 5178 5551 1563 Size: 1288 2327 373 3063 Fragments arranged by size: 3063 2327 1288 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2225 2631 5114 6498 6766 1632 Size: 593 406 2483 1384 268 1917 Fragments arranged by size: 2483 1917 1384 593 406 268 AfeI AGC'GCT Cuts at: 4340 4340 Size: 7051 AflII C'TTAA_G Cuts at: 820 1017 2599 820 Size: 197 1582 5272 Fragments arranged by size: 5272 1582 197 AflIII A'CryG_T Cuts at: 1203 4318 5950 6927 1203 Size: 3115 1632 977 1327 Fragments arranged by size: 3115 1632 1327 977 AgeI A'CCGG_T Cuts at: 5882 5882 Size: 7051 AhdI GACnn_n'nnGTC Cuts at: 5780 5780 Size: 7051 AleI CACnn'nnGTG Cuts at: 1166 2159 3374 1166 Size: 993 1215 4843 Fragments arranged by size: 4843 1215 993 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7051 AlwNI CAG_nnn'CTG Cuts at: 3643 4163 4794 6366 3643 Size: 520 631 1572 4328 Fragments arranged by size: 4328 1572 631 520 ApaI G_GGCC'C Cuts at: 2694 2694 Size: 7051 ApaLI G'TGCA_C Cuts at: 3000 3332 5108 6264 3000 Size: 332 1776 1156 3787 Fragments arranged by size: 3787 1776 1156 332 ApoI r'AATT_y Cuts at: 1493 3445 3554 3623 3777 3787 4199 4360 4586 Size: 1952 109 69 154 10 412 161 226 Cuts at: 4586 4738 6847 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1952 1697 412 226 161 154 152 109 69 10 AseI AT'TA_AT Cuts at: 160 1032 5605 160 Size: 872 4573 1606 Fragments arranged by size: 4573 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7051 AvaI C'yCGr_G Cuts at: 1870 1943 2092 2703 4376 1870 Size: 73 149 611 1673 4545 Fragments arranged by size: 4545 1673 611 149 73 BamHI G'GATC_C Cuts at: 4381 4748 4381 Size: 367 6684 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2153 3537 4238 4306 4372 Size: 325 217 627 366 1384 701 68 66 Cuts at: 4372 5827 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1384 701 627 366 325 217 68 66 BanII G_rGCy'C Cuts at: 1391 2631 2694 4370 1391 Size: 1240 63 1676 4072 Fragments arranged by size: 4072 1676 1240 63 BbeI G_GCGC'C Cuts at: 4310 4310 Size: 7051 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4107 928 Size: 971 2208 3872 Fragments arranged by size: 3872 2208 971 BbvCI CC'TCA_GC Cuts at: 3528 3528 Size: 7051 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5242 5276 1903 Size: 34 3305 34 3678 Fragments arranged by size: 3678 3305 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4003 4943 6153 4003 Size: 940 1210 4901 Fragments arranged by size: 4901 1210 940 BclI T'GATC_A Cuts at: 1548 3645 4071 1548 Size: 2097 426 4528 Fragments arranged by size: 4528 2097 426 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5662 136 Size: 107 122 71 631 605 3990 1525 Fragments arranged by size: 3990 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3168 3879 7046 3168 Size: 711 3167 3173 Fragments arranged by size: 3173 3167 711 BlpI GC'TnA_GC Cuts at: 2432 4468 2432 Size: 2036 5015 Bme1580I G_kGCm'C Cuts at: 1790 2694 3004 3336 3542 5112 6268 1790 Size: 904 310 332 206 1570 1156 2573 Fragments arranged by size: 2573 1570 1156 904 332 310 206 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7051 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3127 4782 5740 Size: 416 53 83 399 24 1710 1655 958 Cuts at: 5740 442 Size: 1753 Fragments arranged by size: 1753 1710 1655 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7051 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4306 4306 Size: 7051 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2206 2259 2463 2555 2828 2885 3767 3883 Size: 967 53 204 92 273 57 882 116 Cuts at: 3883 4192 5711 1239 Size: 309 1519 2579 Fragments arranged by size: 2579 1519 967 882 309 273 204 116 92 57 53 Bpu10I CC'TnA_GC Cuts at: 3462 3528 3462 Size: 66 6985 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3278 5176 6041 6339 3278 Size: 1898 865 298 3990 Fragments arranged by size: 3990 1898 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2010 5714 882 Size: 617 511 3704 2219 Fragments arranged by size: 3704 2219 617 511 BsaAI yAC'GTr Cuts at: 493 1204 4842 493 Size: 711 3638 2702 Fragments arranged by size: 3638 2702 711 BsaBI GATnn'nnATC Cuts at: 7045 7045 Size: 7051 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2088 3904 4307 4858 Size: 53 83 186 936 555 1816 403 551 Cuts at: 4858 5240 5875 275 Size: 382 635 1451 Fragments arranged by size: 1816 1451 936 635 555 551 403 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2895 5484 5882 6156 6303 6714 2895 Size: 2589 398 274 147 411 3232 Fragments arranged by size: 3232 2589 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2204 2234 1178 Size: 30 165 30 801 30 5995 Fragments arranged by size: 5995 801 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4109 4193 4196 1186 Size: 2923 84 3 4041 Fragments arranged by size: 4041 2923 84 3 BseYI C'CCAG_C Cuts at: 3094 3241 3394 4193 4310 6254 6750 3094 Size: 147 153 799 117 1944 496 3395 Fragments arranged by size: 3395 1944 799 496 153 147 117 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3398 1163 Size: 573 1662 4816 Fragments arranged by size: 4816 1662 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4501 5262 6290 1540 Size: 275 186 2500 761 1028 2301 Fragments arranged by size: 2500 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2631 3004 3336 3369 5112 5197 6268 2631 Size: 373 332 33 1743 85 1071 3414 Fragments arranged by size: 3414 1743 1071 373 332 85 33 BsmI GAATG_Cn' Cuts at: 2597 4562 4655 2597 Size: 1965 93 4993 Fragments arranged by size: 4993 1965 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3762 3762 Size: 7051 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2631 2694 3004 3336 3369 3542 4370 Size: 399 841 63 310 332 33 173 828 Cuts at: 4370 5112 5197 6268 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 841 828 742 399 332 310 173 85 63 33 BspEI T'CCGG_A Cuts at: 2895 6714 2895 Size: 3819 3232 BspHI T'CATG_A Cuts at: 3315 3655 4940 6670 3315 Size: 340 1285 1730 3696 Fragments arranged by size: 3696 1730 1285 340 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2128 4406 844 Size: 1284 2278 3489 Fragments arranged by size: 3489 2278 1284 BsrBI CCG'CTC Cuts at: 1394 1823 2024 4330 4938 1394 Size: 429 201 2306 608 3507 Fragments arranged by size: 3507 2306 608 429 201 BsrDI GCAATG_nn' Cuts at: 65 1248 5546 5720 65 Size: 1183 4298 174 1396 Fragments arranged by size: 4298 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3174 5695 5882 1700 Size: 110 1364 2521 187 2869 Fragments arranged by size: 2869 2521 1364 187 110 BsrGI T'GTAC_A Cuts at: 96 3603 4275 4321 96 Size: 3507 672 46 2826 Fragments arranged by size: 3507 2826 672 46 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7051 BssSI C'ACGA_G Cuts at: 5111 6123 5111 Size: 1012 6039 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7051 BstBI TT'CG_AA Cuts at: 5855 5855 Size: 7051 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7051 BstXI CCAn_nnnn'nTGG Cuts at: 3484 4168 3484 Size: 684 6367 BstYI r'GATC_y Cuts at: 1972 2713 3168 3879 4211 4381 4748 5133 5150 Size: 741 455 711 332 170 367 385 17 Cuts at: 5150 6602 6688 6700 7046 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 741 711 455 385 367 346 332 170 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2106 2423 2106 Size: 317 6734 BtgI C'CryG_G Cuts at: 513 1065 2004 2739 3889 513 Size: 552 939 735 1150 3675 Fragments arranged by size: 3675 1150 939 735 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3493 4278 487 Size: 683 56 2267 785 3260 Fragments arranged by size: 3260 2267 785 683 56 BtsI GCAGTG_nn' Cuts at: 2689 3128 4567 5361 5381 2689 Size: 439 1439 794 20 4359 Fragments arranged by size: 4359 1439 794 439 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7051 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7016 DraI TTT'AAA Cuts at: 3444 4355 3444 Size: 911 6140 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6058 809 Size: 507 4742 1802 Fragments arranged by size: 4742 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2248 4498 5387 Size: 54 1425 50 275 18 418 2250 889 Cuts at: 5387 7016 8 Size: 1629 43 Fragments arranged by size: 2250 1629 1425 889 418 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4498 1537 Size: 275 2686 4090 Fragments arranged by size: 4090 2686 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2064 2076 2787 2963 3957 4119 4337 4981 Size: 711 12 711 176 994 162 218 644 Cuts at: 4981 1353 Size: 3423 Fragments arranged by size: 3423 994 711 711 644 218 176 162 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5638 6012 6158 387 Size: 1064 138 4049 374 146 1280 Fragments arranged by size: 4049 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2629 2629 Size: 7051 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2206 2225 2259 2463 2555 2631 2828 Size: 393 574 19 34 204 92 76 197 Cuts at: 2828 2885 3767 3883 4192 5114 5711 6498 6766 Size: 57 882 116 309 922 597 787 268 Cuts at: 6766 1239 Size: 1524 Fragments arranged by size: 1524 922 882 787 597 574 393 309 268 204 197 116 92 76 57 34 19 EcoO109I rG'GnC_Cy Cuts at: 2226 2575 2691 3261 3310 2226 Size: 349 116 570 49 5967 Fragments arranged by size: 5967 570 349 116 49 EcoRI G'AATT_C Cuts at: 4360 4738 4360 Size: 378 6673 EcoRV GAT'ATC Cuts at: 2920 2920 Size: 7051 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2527 2527 Size: 7051 FspI TGC'GCA Cuts at: 5557 5557 Size: 7051 HaeII r_GCGC'y Cuts at: 1678 4234 4310 4342 6198 1678 Size: 2556 76 32 1856 2531 Fragments arranged by size: 2556 2531 1856 76 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2204 2236 5692 5724 5766 Size: 32 135 32 632 32 3456 32 42 Cuts at: 5766 5798 1373 Size: 32 2626 Fragments arranged by size: 3456 2626 632 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2911 4395 4641 669 Size: 988 281 973 1484 246 3079 Fragments arranged by size: 3079 1484 988 973 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7051 HpaI GTT'AAC Cuts at: 4641 4641 Size: 7051 KasI G'GCGC_C Cuts at: 4306 4306 Size: 7051 KpnI G_GTAC'C Cuts at: 4376 4376 Size: 7051 MfeI C'AATT_G Cuts at: 4650 4650 Size: 7051 MluI A'CGCG_T Cuts at: 6927 6927 Size: 7051 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6165 6349 1305 Size: 4860 184 2007 Fragments arranged by size: 4860 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2250 10 Size: 54 1425 343 418 4811 Fragments arranged by size: 4811 1425 418 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2159 2343 2550 2738 3374 5009 5368 Size: 648 993 184 207 188 636 1635 359 Cuts at: 5368 5527 518 Size: 159 2042 Fragments arranged by size: 2042 1635 993 648 636 359 207 188 184 159 MspA1I CmG'CkG Cuts at: 1606 2203 2381 2945 3107 4467 5144 6292 6537 Size: 597 178 564 162 1360 677 1148 245 Cuts at: 6537 1606 Size: 2120 Fragments arranged by size: 2120 1360 1148 677 597 564 245 178 162 NarI GG'CG_CC Cuts at: 4307 4307 Size: 7051 NcoI C'CATG_G Cuts at: 513 1065 2004 2739 3889 513 Size: 552 939 735 1150 3675 Fragments arranged by size: 3675 1150 939 735 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7051 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7051 NotI GC'GGCC_GC Cuts at: 4498 4498 Size: 7051 NspI r_CATG'y Cuts at: 1132 1229 3069 4322 4409 5954 1132 Size: 97 1840 1253 87 1545 2229 Fragments arranged by size: 2229 1840 1545 1253 97 87 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7051 PciI A'CATG_T Cuts at: 4318 5950 4318 Size: 1632 5419 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7051 PfoI T'CCnGG_A Cuts at: 2045 2571 3321 2045 Size: 526 750 5775 Fragments arranged by size: 5775 750 526 PmeI GTTT'AAAC Cuts at: 4355 4355 Size: 7051 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5120 5152 6633 6665 5120 Size: 32 1481 32 5506 Fragments arranged by size: 5506 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2575 3261 2575 Size: 686 6365 PshAI GACnn'nnGTC Cuts at: 1613 2444 3413 3754 1613 Size: 831 969 341 4910 Fragments arranged by size: 4910 969 831 341 PsiI TTA'TAA Cuts at: 4621 4621 Size: 7051 PspOMI G'GGCC_C Cuts at: 2690 2690 Size: 7051 PstI C_TGCA'G Cuts at: 830 3495 4403 830 Size: 2665 908 3478 Fragments arranged by size: 3478 2665 908 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7051 PvuII CAG'CTG Cuts at: 2381 2945 3107 2381 Size: 564 162 6325 Fragments arranged by size: 6325 564 162 RsrII CG'GwC_CG Cuts at: 2026 2026 Size: 7051 SacI G_AGCT'C Cuts at: 2631 2631 Size: 7051 SalI G'TCGA_C Cuts at: 1936 4393 1936 Size: 2457 4594 SapI GCTCTTCn'nnn_ Cuts at: 2963 4119 4337 2963 Size: 1156 218 5677 Fragments arranged by size: 5677 1156 218 SbfI CC_TGCA'GG Cuts at: 4403 4403 Size: 7051 ScaI AGT'ACT Cuts at: 5299 7011 5299 Size: 1712 5339 SfcI C'TryA_G Cuts at: 826 1045 2185 3491 4399 5534 6215 6406 826 Size: 219 1140 1306 908 1135 681 191 1471 Fragments arranged by size: 1471 1306 1140 1135 908 681 219 191 SfoI GGC'GCC Cuts at: 4308 4308 Size: 7051 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7051 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7051 SmaI CCC'GGG Cuts at: 1872 2094 4378 1872 Size: 222 2284 4545 Fragments arranged by size: 4545 2284 222 SmlI C'TyrA_G Cuts at: 820 1017 1943 2599 3293 5155 6056 6318 820 Size: 197 926 656 694 1862 901 262 1553 Fragments arranged by size: 1862 1553 926 901 694 656 262 197 SnaBI TAC'GTA Cuts at: 493 4842 493 Size: 4349 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7051 SphI G_CATG'C Cuts at: 1132 3069 4409 1132 Size: 1937 1340 3774 Fragments arranged by size: 3774 1937 1340 SspI AAT'ATT Cuts at: 5 52 4975 5 Size: 47 4923 2081 Fragments arranged by size: 4923 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7051 StyI C'CwwG_G Cuts at: 513 1065 2004 2178 2739 3192 3828 3889 4490 Size: 552 939 174 561 453 636 61 601 Cuts at: 4490 513 Size: 3074 Fragments arranged by size: 3074 939 636 601 561 552 453 174 61 SwaI ATTT'AAAT Cuts at: 3444 3444 Size: 7051 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2034 2224 5078 5095 5248 5433 5878 Size: 1255 454 190 2854 17 153 185 445 Cuts at: 5878 325 Size: 1498 Fragments arranged by size: 2854 1498 1255 454 445 190 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2349 2480 3603 Size: 274 80 33 51 1443 372 131 1123 Cuts at: 3603 3966 4275 4321 5297 7009 96 Size: 363 309 46 976 1712 138 Fragments arranged by size: 1712 1443 1123 976 372 363 309 274 138 131 80 51 46 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7011 7043 7011 Size: 32 7019 Tth111I GACn'n_nGTC Cuts at: 1316 2806 1316 Size: 1490 5561 XbaI T'CTAG_A Cuts at: 4387 4387 Size: 7051 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7051 XmaI C'CCGG_G Cuts at: 1870 2092 4376 1870 Size: 222 2284 4545 Fragments arranged by size: 4545 2284 222 XmnI GAAnn'nnTTC Cuts at: 2115 5180 2115 Size: 3065 3986 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4859 276 Size: 53 83 186 936 3325 2468 Fragments arranged by size: 3325 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI SacII SanDI SexAI SfiI SrfI XcmI