Restriction map for
pFN21AB3475
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BciVI BclI BfrI
BglII BlnI BsaAI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstBI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HindIII KasI KpnI KspI
MfeI MflI MluI MroI MunI
NarI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
Tth111I XbaI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AfaI 393 GTAC GT^AC
AlwI 366 GATCC GGATC(4/5)
AlwNI 844 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 461 GGGCCC GGGCC^C
BanI 469 GGCACC G^G[CT][GA]CC
BfaI 1063 CTAG C^TAG
BglI 1019 GCCTCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 567 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 567 GCTGAGC GC^T[ACGT]AGC
BsaBI 360 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 937 TCCGGT [AT]^CCGG[AT]
BsmAI 1360 GAGAC GTCTC(1/5)
BspHI 1049 TCATGA T^CATGA
BsrBI 789 CCGCTC CCGCTC(-3/-3)
BsrDI 1209 CATTGC GCAATG(2/0)
BssSI 882 CACGAG CACGAG(-5/-1)
BstAPI 747 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 768 GGTCACC G^GT[ACGT]ACC
BstPI 768 GGTCACC G^GT[ACGT]ACC
CelII 567 GCTGAGC GC^T[ACGT]AGC
EcoO65I 768 GGTCACC G^GT[ACGT]ACC
EcoRI 279 GAATTC G^AATTC
EcoT14I 1159 CCATGG C^C[AT][AT]GG
HpaI 259 GTTAAC GTT^AAC
MaeI 1063 CTAG C^TAG
MamI 360 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 260 TTAA T^TAA
NcoI 1159 CCATGG C^CATGG
NheI 1062 GCTAGC G^CTAGC
NspI 1256 GCATGT [GA]CATG^[CT]
PshAI 138 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1049 TCATGA T^CATGA
RsaI 393 GTAC GT^AC
SbfI 720 CCTGCAGG CCTGCA^GG
Sse8387I 720 CCTGCAGG CCTGCA^GG
StuI 1017 AGGCCT AGG^CCT
StyI 1159 CCATGG C^C[AT][AT]GG
TfiI 1465 GATTC G^A[AT]TC
Tru9I 260 TTAA T^TAA
XcmI 843 CCAGCAGCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 694 CGCG CG^CG
1059 CGCG
AcsI 279 GAATTC [GA]^AATT[CT]
1242 AAATTT
ApoI 279 GAATTC [GA]^AATT[CT]
1242 AAATTT
AspHI 322 GTGCTC G[AT]GC[AT]^C
1315 GTGCTC
AvaI 622 CCCGAG C^[CT]CG[GA]G
1435 CCCGAG
BalI 206 TGGCCA TGG^CCA
1109 TGGCCA
BanII 4 GAGCCC G[GA]GC[CT]^C
461 GGGCCC
BbsI 868 GTCTTC GAAGAC(2/6)
1297 GTCTTC
BcgI 488 GCATCGTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
500 GCATCCTCATCG
BcnI 585 CCCGG CC^[GC]GG
666 CCGGG
BpuAI 868 GTCTTC GAAGAC(2/6)
1297 GTCTTC
BsgI 475 CTGCAC GTGCAG(16/14)
907 GTGCAG
BsiHKAI 322 GTGCTC G[AT]GC[AT]^C
1315 GTGCTC
BsoBI 622 CCCGAG C^[CT]CG[GA]G
1435 CCCGAG
BspMI 299 ACCTGC ACCTGC(4/8)
719 ACCTGC
BsrFI 272 GCCGGC [GA]^CCGG[CT]
995 GCCGGC
BstUI 694 CGCG CG^CG
1059 CGCG
BstXI 147 CCACCGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
422 CCATGTATGTGG
CfoI 693 GCGC GCG^C
1060 GCGC
Cfr10I 272 GCCGGC [GA]^CCGG[CT]
995 GCCGGC
DsaI 44 CCGTGG C^C[GA][CT]GG
1159 CCATGG
EarI 1180 CTCTTC CTCTTC(1/4)
736 GAAGAG
HhaI 693 GCGC GCG^C
1060 GCGC
HinP1I 693 GCGC G^CGC
1060 GCGC
HincII 259 GTTAAC GT[CT]^[GA]AC
946 GTCAAC
HindII 259 GTTAAC GT[CT]^[GA]AC
946 GTCAAC
Ksp632I 1180 CTCTTC CTCTTC(1/4)
736 GAAGAG
MluNI 206 TGGCCA TGG^CCA
1109 TGGCCA
MscI 206 TGGCCA TGG^CCA
1109 TGGCCA
MvnI 694 CGCG CG^CG
1059 CGCG
NaeI 272 GCCGGC GCC^GGC
995 GCCGGC
NciI 585 CCCGG CC^[GC]GG
666 CCGGG
NgoMIV 272 GCCGGC G^CCGGC
995 GCCGGC
PpuMI 548 AGGACCT [GA]G^G[AT]CC[CT]
1143 GGGTCCT
Tsp509I 280 AATT ^AATT
1243 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AvaII BbvI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PflMI PleI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I