(Circular) (Six-base) MAPSORT of: pFN21AB3475.seq Check: 3898 from: 1 to: 6181 pFN21AB3475 6181 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3991 278 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3524 1937 Size: 1587 4594 Acc65I G'GTAC_C Cuts at: 3502 3502 Size: 6181 AclI AA'CG_TT Cuts at: 1563 4308 4681 1563 Size: 2745 373 3063 Fragments arranged by size: 3063 2745 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3172 4244 5628 5896 1632 Size: 1540 1072 1384 268 1917 Fragments arranged by size: 1917 1540 1384 1072 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5984 AflIII A'CryG_T Cuts at: 1203 5080 6057 1203 Size: 3877 977 1327 Fragments arranged by size: 3877 1327 977 AgeI A'CCGG_T Cuts at: 5012 5012 Size: 6181 AhdI GACnn_n'nnGTC Cuts at: 4910 4910 Size: 6181 AleI CACnn'nnGTG Cuts at: 1166 2969 1166 Size: 1803 4378 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2630 1184 Size: 1446 4735 AlwNI CAG_nnn'CTG Cuts at: 2854 3924 5496 2854 Size: 1070 1572 3539 Fragments arranged by size: 3539 1572 1070 ApaI G_GGCC'C Cuts at: 2470 2470 Size: 6181 ApaLI G'TGCA_C Cuts at: 4238 5394 4238 Size: 1156 5025 ApoI r'AATT_y Cuts at: 1493 2284 3247 3490 3716 3868 5977 1493 Size: 791 963 243 226 152 2109 1697 Fragments arranged by size: 2109 1697 963 791 243 226 152 AseI AT'TA_AT Cuts at: 160 1032 4735 160 Size: 872 3703 1606 Fragments arranged by size: 3703 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 AvaI C'yCGr_G Cuts at: 1870 1943 2627 3440 3506 1870 Size: 73 684 813 66 4545 Fragments arranged by size: 4545 813 684 73 66 BamHI G'GATC_C Cuts at: 3511 3878 3511 Size: 367 5814 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2474 3502 4957 618 Size: 325 217 627 687 1028 1455 1842 Fragments arranged by size: 1842 1455 1028 687 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2470 3500 1391 Size: 622 457 1030 4072 Fragments arranged by size: 4072 1030 622 457 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2866 3295 928 Size: 971 967 429 3814 Fragments arranged by size: 3814 971 967 429 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2482 2494 2516 2528 4372 4406 1903 Size: 34 545 12 22 12 1844 34 3678 Fragments arranged by size: 3678 1844 545 34 34 22 12 12 BciVI GTATCCnnnnn_n' Cuts at: 4073 5283 4073 Size: 1210 4971 BclI T'GATC_A Cuts at: 1548 1548 Size: 6181 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3030 4792 136 Size: 107 122 71 631 605 1358 1762 1525 Fragments arranged by size: 1762 1525 1358 631 605 122 107 71 BglII A'GATC_T Cuts at: 6176 6176 Size: 6181 BlpI GC'TnA_GC Cuts at: 2573 3598 2573 Size: 1025 5156 Bme1580I G_kGCm'C Cuts at: 1790 2427 2470 2477 4242 5398 1790 Size: 637 43 7 1765 1156 2573 Fragments arranged by size: 2573 1765 1156 637 43 7 BmgBI CAC'GTC Cuts at: 1318 2713 3203 1318 Size: 1395 490 4296 Fragments arranged by size: 4296 1395 490 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2357 3912 4870 Size: 416 53 83 399 24 940 1555 958 Cuts at: 4870 442 Size: 1753 Fragments arranged by size: 1753 1555 958 940 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3071 1056 Size: 2015 4166 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2115 2804 3098 4841 1239 Size: 876 689 294 1743 2579 Fragments arranged by size: 2579 1743 876 689 294 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4306 5171 5469 4306 Size: 865 298 5018 Fragments arranged by size: 5018 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4844 882 Size: 617 3345 2219 Fragments arranged by size: 3345 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3972 493 Size: 711 2768 2702 Fragments arranged by size: 2768 2702 711 BsaBI GATnn'nnATC Cuts at: 2369 6175 2369 Size: 3806 2375 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3988 4370 5005 275 Size: 53 83 186 936 2455 382 635 1451 Fragments arranged by size: 2455 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2942 4614 5012 5286 5433 5844 2942 Size: 1672 398 274 147 411 3279 Fragments arranged by size: 3279 1672 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2046 2076 3272 3302 1178 Size: 30 165 30 643 30 1196 30 4057 Fragments arranged by size: 4057 1196 643 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2070 2141 2678 3458 1186 Size: 884 71 537 780 3909 Fragments arranged by size: 3909 884 780 537 71 BseYI C'CCAG_C Cuts at: 2012 2488 2847 5384 5880 2012 Size: 476 359 2537 496 2313 Fragments arranged by size: 2537 2313 496 476 359 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2465 2933 1163 Size: 573 729 468 4411 Fragments arranged by size: 4411 729 573 468 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3631 4392 5420 1540 Size: 275 186 1630 761 1028 2301 Fragments arranged by size: 2301 1630 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2331 3324 4242 4327 5398 2331 Size: 993 918 85 1071 3114 Fragments arranged by size: 3114 1071 993 918 85 BsmI GAATG_Cn' Cuts at: 3692 3785 3692 Size: 93 6088 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2331 2427 2470 2477 3324 3500 Size: 399 223 318 96 43 7 847 176 Cuts at: 3500 4242 4327 5398 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 847 742 399 318 223 176 96 85 43 7 BspEI T'CCGG_A Cuts at: 5844 5844 Size: 6181 BspHI T'CATG_A Cuts at: 3054 4070 5800 3054 Size: 1016 1730 3435 Fragments arranged by size: 3435 1730 1016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2313 2733 3536 844 Size: 1469 420 803 3489 Fragments arranged by size: 3489 1469 803 420 BsrBI CCG'CTC Cuts at: 1394 1823 2796 4068 1394 Size: 429 973 1272 3507 Fragments arranged by size: 3507 1272 973 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3213 4676 4850 65 Size: 1183 1965 1463 174 1396 Fragments arranged by size: 1965 1463 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2277 3000 4825 5012 1700 Size: 110 467 723 1825 187 2869 Fragments arranged by size: 2869 1825 723 467 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6181 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6181 BssSI C'ACGA_G Cuts at: 2887 4241 5253 2887 Size: 1354 1012 3815 Fragments arranged by size: 3815 1354 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2758 794 Size: 1964 4217 BstBI TT'CG_AA Cuts at: 4985 4985 Size: 6181 BstEII G'GTnAC_C Cuts at: 1184 2773 1184 Size: 1589 4592 BstXI CCAn_nnnn'nTGG Cuts at: 2159 2434 2159 Size: 275 5906 BstYI r'GATC_y Cuts at: 1972 3511 3878 4263 4280 5732 5818 5830 6176 Size: 1539 367 385 17 1452 86 12 346 Cuts at: 6176 1972 Size: 1977 Fragments arranged by size: 1977 1539 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2049 3164 513 Size: 552 939 45 1115 3530 Fragments arranged by size: 3530 1115 939 552 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2497 2809 3046 487 Size: 683 56 1271 312 237 3622 Fragments arranged by size: 3622 1271 683 312 237 56 BtsI GCAGTG_nn' Cuts at: 2067 2609 3071 3697 4491 4511 2067 Size: 542 462 626 794 20 3737 Fragments arranged by size: 3737 794 626 542 462 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6181 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6146 DraI TTT'AAA Cuts at: 3485 3485 Size: 6181 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5188 809 Size: 507 3872 1802 Fragments arranged by size: 3872 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2211 2275 Size: 54 1425 50 275 18 216 165 64 Cuts at: 2275 3114 3628 4517 6146 8 Size: 839 514 889 1629 43 Fragments arranged by size: 1629 1425 889 839 514 275 216 165 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3628 1537 Size: 275 1816 4090 Fragments arranged by size: 4090 1816 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2736 3191 4111 1353 Size: 1383 455 920 3423 Fragments arranged by size: 3423 1383 920 455 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2296 2311 3019 4768 5142 5288 Size: 1064 138 707 15 708 1749 374 146 Cuts at: 5288 387 Size: 1280 Fragments arranged by size: 1749 1280 1064 708 707 374 146 138 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2804 3098 3172 4244 4841 5628 Size: 393 483 689 294 74 1072 597 787 Cuts at: 5628 5896 1239 Size: 268 1524 Fragments arranged by size: 1524 1072 787 689 597 483 393 294 268 74 EcoO109I rG'GnC_Cy Cuts at: 2466 2467 2554 3149 2466 Size: 1 87 595 5498 Fragments arranged by size: 5498 595 87 1 EcoRI G'AATT_C Cuts at: 2284 3490 3868 2284 Size: 1206 378 4597 Fragments arranged by size: 4597 1206 378 FspI TGC'GCA Cuts at: 4687 4687 Size: 6181 HaeII r_GCGC'y Cuts at: 1678 5328 1678 Size: 3650 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2998 3030 3085 3117 4822 Size: 32 135 32 1426 32 55 32 1705 Cuts at: 4822 4854 4896 4928 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1705 1426 135 55 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2266 2953 3525 3771 669 Size: 988 281 328 687 572 246 3079 Fragments arranged by size: 3079 988 687 572 328 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6181 HpaI GTT'AAC Cuts at: 2266 3771 2266 Size: 1505 4676 KpnI G_GTAC'C Cuts at: 3506 3506 Size: 6181 MfeI C'AATT_G Cuts at: 3780 3780 Size: 6181 MluI A'CGCG_T Cuts at: 6057 6057 Size: 6181 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3258 5295 5479 1305 Size: 1953 2037 184 2007 Fragments arranged by size: 2037 2007 1953 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2213 3116 10 Size: 54 1425 343 381 903 3075 Fragments arranged by size: 3075 1425 903 381 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2157 2393 2432 2828 2969 3053 4139 Size: 648 991 236 39 396 141 84 1086 Cuts at: 4139 4498 4657 518 Size: 359 159 2042 Fragments arranged by size: 2042 1086 991 648 396 359 236 159 141 84 39 MspA1I CmG'CkG Cuts at: 1606 2488 2708 2842 2854 3209 3597 4274 5422 Size: 882 220 134 12 355 388 677 1148 Cuts at: 5422 5667 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 882 677 388 355 245 220 134 12 NaeI GCC'GGC Cuts at: 2279 3002 2279 Size: 723 5458 NcoI C'CATG_G Cuts at: 513 1065 2004 3164 513 Size: 552 939 1160 3530 Fragments arranged by size: 3530 1160 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6181 NgoMIV G'CCGG_C Cuts at: 2277 3000 2277 Size: 723 5458 NheI G'CTAG_C Cuts at: 1052 3067 1052 Size: 2015 4166 NotI GC'GGCC_GC Cuts at: 3628 3628 Size: 6181 NspI r_CATG'y Cuts at: 1132 1229 3265 3539 5084 1132 Size: 97 2036 274 1545 2229 Fragments arranged by size: 2229 2036 1545 274 97 PasI CC'CwG_GG Cuts at: 1348 2470 3460 1348 Size: 1122 990 4069 Fragments arranged by size: 4069 1122 990 PciI A'CATG_T Cuts at: 5080 5080 Size: 6181 PflMI CCAn_nnn'nTGG Cuts at: 1108 2196 3165 3277 3460 1108 Size: 1088 969 112 183 3829 Fragments arranged by size: 3829 1088 969 183 112 PfoI T'CCnGG_A Cuts at: 2781 2781 Size: 6181 PmeI GTTT'AAAC Cuts at: 3485 3485 Size: 6181 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3434 3466 4250 4282 5763 5795 3434 Size: 32 784 32 1481 32 3820 Fragments arranged by size: 3820 1481 784 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2554 3149 2554 Size: 595 5586 PshAI GACnn'nnGTC Cuts at: 1613 2147 1613 Size: 534 5647 PsiI TTA'TAA Cuts at: 3751 3751 Size: 6181 PspOMI G'GGCC_C Cuts at: 2466 2466 Size: 6181 PstI C_TGCA'G Cuts at: 830 2079 2604 2730 2841 3533 830 Size: 1249 525 126 111 692 3478 Fragments arranged by size: 3478 1249 692 525 126 111 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6181 PvuII CAG'CTG Cuts at: 2488 2708 2842 2854 3209 2488 Size: 220 134 12 355 5460 Fragments arranged by size: 5460 355 220 134 12 SalI G'TCGA_C Cuts at: 1936 3523 1936 Size: 1587 4594 SbfI CC_TGCA'GG Cuts at: 2730 3533 2730 Size: 803 5378 ScaI AGT'ACT Cuts at: 4429 6141 4429 Size: 1712 4469 SfcI C'TryA_G Cuts at: 826 1045 2075 2600 2726 2837 3529 4664 5345 Size: 219 1030 525 126 111 692 1135 681 Cuts at: 5345 5536 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1030 692 681 525 219 191 126 111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6181 SmaI CCC'GGG Cuts at: 1872 3508 1872 Size: 1636 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4285 5186 5448 820 Size: 197 926 2342 901 262 1553 Fragments arranged by size: 2342 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3972 493 Size: 3479 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6181 SphI G_CATG'C Cuts at: 1132 3539 1132 Size: 2407 3774 SspI AAT'ATT Cuts at: 5 52 4105 5 Size: 47 4053 2081 Fragments arranged by size: 4053 2081 47 StuI AGG'CCT Cuts at: 1517 3024 1517 Size: 1507 4674 StyI C'CwwG_G Cuts at: 513 1065 2004 3164 3620 513 Size: 552 939 1160 456 3074 Fragments arranged by size: 3074 1160 939 552 456 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2426 2464 4208 4225 4378 4563 5008 Size: 1255 846 38 1744 17 153 185 445 Cuts at: 5008 325 Size: 1498 Fragments arranged by size: 1744 1498 1255 846 445 185 153 38 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2397 4427 6139 Size: 274 80 33 51 1443 420 2030 1712 Cuts at: 6139 96 Size: 138 Fragments arranged by size: 2030 1712 1443 420 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2046 2078 3272 3304 6141 6173 2046 Size: 32 1194 32 2837 32 2054 Fragments arranged by size: 2837 2054 1194 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6181 XbaI T'CTAG_A Cuts at: 3517 3517 Size: 6181 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2855 2855 Size: 6181 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6181 XmaI C'CCGG_G Cuts at: 1870 3506 1870 Size: 1636 4545 XmnI GAAnn'nnTTC Cuts at: 4310 4310 Size: 6181 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3989 276 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI