Restriction map for
pFN21AB3475
(Linear) (Six-base) MAPSORT of: KIBB3475.seq Check: 3591 from: 1 to: 1476
KIBB3475.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1167 1476
Size: 1167 309
AleI CACnn'nnGTG
Cuts at: 0 964 1476
Size: 964 512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 625 1476
Size: 625 851
AlwNI CAG_nnn'CTG
Cuts at: 0 849 1476
Size: 849 627
ApaI G_GGCC'C
Cuts at: 0 465 1476
Size: 465 1011
ApoI r'AATT_y
Cuts at: 0 279 1242 1476
Size: 279 963 234
Fragments arranged by size:
963 279 234
AvaI C'yCGr_G
Cuts at: 0 622 1435 1476
Size: 622 813 41
Fragments arranged by size:
813 622 41
BanI G'GyrC_C
Cuts at: 0 469 1476
Size: 469 1007
BanII G_rGCy'C
Cuts at: 0 8 465 1476
Size: 8 457 1011
Fragments arranged by size:
1011 457 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 861 1290 1476
Size: 861 429 186
Fragments arranged by size:
861 429 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 477 489 511 523 1476
Size: 477 12 22 12 953
Fragments arranged by size:
953 477 22 12 12
BglI GCCn_nnn'nGGC
Cuts at: 0 1025 1476
Size: 1025 451
BlpI GC'TnA_GC
Cuts at: 0 568 1476
Size: 568 908
Bme1580I G_kGCm'C
Cuts at: 0 422 465 472 1476
Size: 422 43 7 1004
Fragments arranged by size:
1004 422 43 7
BmgBI CAC'GTC
Cuts at: 0 708 1198 1476
Size: 708 490 278
Fragments arranged by size:
708 490 278
BmrI ACTGGGnnnn_n'
Cuts at: 0 352 1476
Size: 352 1124
BmtI G_CTAG'C
Cuts at: 0 1066 1476
Size: 1066 410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 799 1093 1476
Size: 110 689 294 383
Fragments arranged by size:
689 383 294 110
BsaBI GATnn'nnATC
Cuts at: 0 364 1476
Size: 364 1112
BsaWI w'CCGG_w
Cuts at: 0 937 1476
Size: 937 539
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 41 71 1267 1297 1476
Size: 41 30 1196 30 179
Fragments arranged by size:
1196 179 41 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 65 136 673 1453 1476
Size: 65 71 537 780 23
Fragments arranged by size:
780 537 71 65 23
BseYI C'CCAG_C
Cuts at: 0 7 483 842 1476
Size: 7 476 359 634
Fragments arranged by size:
634 476 359 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 928 1476
Size: 460 468 548
Fragments arranged by size:
548 468 460
BsiHKAI G_wGCw'C
Cuts at: 0 326 1319 1476
Size: 326 993 157
Fragments arranged by size:
993 326 157
Bsp1286I G_dGCh'C
Cuts at: 0 8 326 422 465 472 1319 1476
Size: 8 318 96 43 7 847 157
Fragments arranged by size:
847 318 157 96 43 8 7
BspHI T'CATG_A
Cuts at: 0 1049 1476
Size: 1049 427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 308 728 1476
Size: 308 420 748
Fragments arranged by size:
748 420 308
BsrBI CCG'CTC
Cuts at: 0 791 1476
Size: 791 685
BsrDI GCAATG_nn'
Cuts at: 0 1208 1476
Size: 1208 268
BsrFI r'CCGG_y
Cuts at: 0 272 995 1476
Size: 272 723 481
Fragments arranged by size:
723 481 272
BssSI C'ACGA_G
Cuts at: 0 882 1476
Size: 882 594
BstAPI GCAn_nnn'nTGC
Cuts at: 0 753 1476
Size: 753 723
BstEII G'GTnAC_C
Cuts at: 0 768 1476
Size: 768 708
BstXI CCAn_nnnn'nTGG
Cuts at: 0 154 429 1476
Size: 154 275 1047
Fragments arranged by size:
1047 275 154
BtgI C'CryG_G
Cuts at: 0 44 1159 1476
Size: 44 1115 317
Fragments arranged by size:
1115 317 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 492 804 1041 1476
Size: 492 312 237 435
Fragments arranged by size:
492 435 312 237
BtsI GCAGTG_nn'
Cuts at: 0 62 604 1066 1476
Size: 62 542 462 410
Fragments arranged by size:
542 462 410 62
EaeI y'GGCC_r
Cuts at: 0 41 206 270 1109 1476
Size: 41 165 64 839 367
Fragments arranged by size:
839 367 165 64 41
EarI CTCTTCn'nnn_
Cuts at: 0 731 1186 1476
Size: 731 455 290
Fragments arranged by size:
731 455 290
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 291 306 1014 1476
Size: 291 15 708 462
Fragments arranged by size:
708 462 291 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 799 1093 1167 1476
Size: 110 689 294 74 309
Fragments arranged by size:
689 309 294 110 74
EcoO109I rG'GnC_Cy
Cuts at: 0 461 462 549 1144 1476
Size: 461 1 87 595 332
Fragments arranged by size:
595 461 332 87 1
EcoRI G'AATT_C
Cuts at: 0 279 1476
Size: 279 1197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 993 1025 1080 1112 1476
Size: 993 32 55 32 364
Fragments arranged by size:
993 364 55 32 32
HincII GTy'rAC
Cuts at: 0 261 948 1476
Size: 261 687 528
Fragments arranged by size:
687 528 261
HpaI GTT'AAC
Cuts at: 0 261 1476
Size: 261 1215
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1253 1476
Size: 1253 223
MscI TGG'CCA
Cuts at: 0 208 1111 1476
Size: 208 903 365
Fragments arranged by size:
903 365 208
MslI CAynn'nnrTG
Cuts at: 0 152 388 427 823 964 1048 1476
Size: 152 236 39 396 141 84 428
Fragments arranged by size:
428 396 236 152 141 84 39
MspA1I CmG'CkG
Cuts at: 0 483 703 837 849 1204 1476
Size: 483 220 134 12 355 272
Fragments arranged by size:
483 355 272 220 134 12
NaeI GCC'GGC
Cuts at: 0 274 997 1476
Size: 274 723 479
Fragments arranged by size:
723 479 274
NcoI C'CATG_G
Cuts at: 0 1159 1476
Size: 1159 317
NgoMIV G'CCGG_C
Cuts at: 0 272 995 1476
Size: 272 723 481
Fragments arranged by size:
723 481 272
NheI G'CTAG_C
Cuts at: 0 1062 1476
Size: 1062 414
NspI r_CATG'y
Cuts at: 0 1260 1476
Size: 1260 216
PasI CC'CwG_GG
Cuts at: 0 465 1455 1476
Size: 465 990 21
Fragments arranged by size:
990 465 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 191 1160 1272 1455 1476
Size: 191 969 112 183 21
Fragments arranged by size:
969 191 183 112 21
PfoI T'CCnGG_A
Cuts at: 0 776 1476
Size: 776 700
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1429 1461 1476
Size: 1429 32 15
Fragments arranged by size:
1429 32 15
PpuMI rG'GwC_Cy
Cuts at: 0 549 1144 1476
Size: 549 595 332
Fragments arranged by size:
595 549 332
PshAI GACnn'nnGTC
Cuts at: 0 142 1476
Size: 142 1334
PspOMI G'GGCC_C
Cuts at: 0 461 1476
Size: 461 1015
PstI C_TGCA'G
Cuts at: 0 74 599 725 836 1476
Size: 74 525 126 111 640
Fragments arranged by size:
640 525 126 111 74
PvuII CAG'CTG
Cuts at: 0 483 703 837 849 1204 1476
Size: 483 220 134 12 355 272
Fragments arranged by size:
483 355 272 220 134 12
SbfI CC_TGCA'GG
Cuts at: 0 725 1476
Size: 725 751
SfcI C'TryA_G
Cuts at: 0 70 595 721 832 1476
Size: 70 525 126 111 644
Fragments arranged by size:
644 525 126 111 70
StuI AGG'CCT
Cuts at: 0 1019 1476
Size: 1019 457
StyI C'CwwG_G
Cuts at: 0 1159 1476
Size: 1159 317
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 421 459 1476
Size: 421 38 1017
Fragments arranged by size:
1017 421 38
TatI w'GTAC_w
Cuts at: 0 392 1476
Size: 392 1084
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 41 73 1267 1299 1476
Size: 41 32 1194 32 177
Fragments arranged by size:
1194 177 41 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 850 1476
Size: 850 626
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI ApoI AvaI BanI
BanII BbsI BcgI BglI BlpI Bme1580I BmgBI BmrI
BmtI BpmI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BtgI BtgZI BtsI EaeI EarI
EciI Eco57MI EcoO109I EcoRI Hin4I HincII HpaI MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NheI NspI
PasI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI
PvuII SbfI SfcI StuI StyI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglII
BplI Bpu10I BpuEI BsaI BsaAI BsaHI BsiEI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstBI BstYI BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HindIII
KasI KpnI MfeI MluI NarI NdeI NotI NruI
NsiI PacI PciI PmeI PmlI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI