(Circular) (Six-base) MAPSORT of: pFN21AB3473.seq Check: 1237 from: 1 to: 6196 pFN21AB3473 6196 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2603 2603 Size: 6196 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4006 278 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2210 3539 1937 Size: 273 1329 4594 Fragments arranged by size: 4594 1329 273 Acc65I G'GTAC_C Cuts at: 3517 3517 Size: 6196 AclI AA'CG_TT Cuts at: 1563 2516 4323 4696 1563 Size: 953 1807 373 3063 Fragments arranged by size: 3063 1807 953 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4259 5643 5911 1632 Size: 2627 1384 268 1917 Fragments arranged by size: 2627 1917 1384 268 AfeI AGC'GCT Cuts at: 3007 3007 Size: 6196 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5999 AflIII A'CryG_T Cuts at: 1203 3483 5095 6072 1203 Size: 2280 1612 977 1327 Fragments arranged by size: 2280 1612 1327 977 AgeI A'CCGG_T Cuts at: 5027 5027 Size: 6196 AhdI GACnn_n'nnGTC Cuts at: 4925 4925 Size: 6196 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6196 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2765 1184 Size: 1581 4615 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2866 2898 2866 Size: 32 6164 AlwNI CAG_nnn'CTG Cuts at: 3939 5511 3939 Size: 1572 4624 ApaLI G'TGCA_C Cuts at: 4253 5409 4253 Size: 1156 5040 ApoI r'AATT_y Cuts at: 1493 2707 3505 3731 3883 5992 1493 Size: 1214 798 226 152 2109 1697 Fragments arranged by size: 2109 1697 1214 798 226 152 AseI AT'TA_AT Cuts at: 160 1032 2087 3156 4750 160 Size: 872 1055 1069 1594 1606 Fragments arranged by size: 1606 1594 1069 1055 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 AvaI C'yCGr_G Cuts at: 1870 1943 3204 3521 1870 Size: 73 1261 317 4545 Fragments arranged by size: 4545 1261 317 73 AvrII C'CTAG_G Cuts at: 3088 3088 Size: 6196 BamHI G'GATC_C Cuts at: 3388 3526 3893 3388 Size: 138 367 5691 Fragments arranged by size: 5691 367 138 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3517 4972 618 Size: 325 217 627 1730 1455 1842 Fragments arranged by size: 1842 1730 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2300 2584 3515 1391 Size: 909 284 931 4072 Fragments arranged by size: 4072 931 909 284 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5225 BbvCI CC'TCA_GC Cuts at: 3104 3104 Size: 6196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4387 4421 1903 Size: 34 2450 34 3678 Fragments arranged by size: 3678 2450 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4088 5298 4088 Size: 1210 4986 BclI T'GATC_A Cuts at: 1548 1548 Size: 6196 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4807 136 Size: 107 122 71 631 605 3135 1525 Fragments arranged by size: 3135 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2812 6191 2812 Size: 3379 2817 BlpI GC'TnA_GC Cuts at: 3613 3613 Size: 6196 Bme1580I G_kGCm'C Cuts at: 1790 4257 5413 1790 Size: 2467 1156 2573 Fragments arranged by size: 2573 2467 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6196 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2906 3927 4885 Size: 416 53 83 399 24 1489 1021 958 Cuts at: 4885 442 Size: 1753 Fragments arranged by size: 1753 1489 1021 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2181 1056 Size: 1125 5071 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2598 3141 4856 1239 Size: 1359 543 1715 2579 Fragments arranged by size: 2579 1715 1359 543 Bpu10I CC'TnA_GC Cuts at: 2737 3104 2737 Size: 367 5829 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2189 2677 2871 4321 5186 5484 2189 Size: 488 194 1450 865 298 2901 Fragments arranged by size: 2901 1450 865 488 298 194 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2584 4859 882 Size: 617 1085 2275 2219 Fragments arranged by size: 2275 2219 1085 617 BsaAI yAC'GTr Cuts at: 493 1204 3987 493 Size: 711 2783 2702 Fragments arranged by size: 2783 2702 711 BsaBI GATnn'nnATC Cuts at: 6190 6190 Size: 6196 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4003 4385 5020 275 Size: 53 83 186 936 2470 382 635 1451 Fragments arranged by size: 2470 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4629 5027 5301 5448 5859 4629 Size: 398 274 147 411 4966 Fragments arranged by size: 4966 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2746 2776 1178 Size: 30 165 30 1343 30 4598 Fragments arranged by size: 4598 1343 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2574 3192 1186 Size: 1388 618 4190 Fragments arranged by size: 4190 1388 618 BseYI C'CCAG_C Cuts at: 3055 3285 3306 5399 5895 3055 Size: 230 21 2093 496 3356 Fragments arranged by size: 3356 2093 496 230 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5623 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3646 4407 5435 1540 Size: 275 186 1645 761 1028 2301 Fragments arranged by size: 2301 1645 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 2325 2584 2630 4257 4342 5413 2118 Size: 207 259 46 1627 85 1071 2901 Fragments arranged by size: 2901 1627 1071 259 207 85 46 BsmI GAATG_Cn' Cuts at: 3051 3707 3800 3051 Size: 656 93 5447 Fragments arranged by size: 5447 656 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2300 2325 2584 2630 3515 4257 Size: 399 328 182 25 259 46 885 742 Cuts at: 4257 4342 5413 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 885 742 399 328 259 182 85 46 25 BspEI T'CCGG_A Cuts at: 5859 5859 Size: 6196 BspHI T'CATG_A Cuts at: 2230 4085 5815 2230 Size: 1855 1730 2611 Fragments arranged by size: 2611 1855 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2603 3551 844 Size: 1759 948 3489 Fragments arranged by size: 3489 1759 948 BsrBI CCG'CTC Cuts at: 1394 1823 4083 1394 Size: 429 2260 3507 Fragments arranged by size: 3507 2260 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2614 2973 4691 4865 65 Size: 1183 1366 359 1718 174 1396 Fragments arranged by size: 1718 1396 1366 1183 359 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4840 5027 1700 Size: 110 3030 187 2869 Fragments arranged by size: 3030 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6196 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6196 BssSI C'ACGA_G Cuts at: 4256 5268 4256 Size: 1012 5184 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6196 BstBI TT'CG_AA Cuts at: 5000 5000 Size: 6196 BstEII G'GTnAC_C Cuts at: 1184 3490 1184 Size: 2306 3890 BstYI r'GATC_y Cuts at: 1972 2812 3388 3526 3893 4278 4295 5747 5833 Size: 840 576 138 367 385 17 1452 86 Cuts at: 5833 5845 6191 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 840 576 385 367 346 138 86 17 12 BstZ17I GTA'TAC Cuts at: 2211 2211 Size: 6196 BtgI C'CryG_G Cuts at: 513 1065 2004 3392 513 Size: 552 939 1388 3317 Fragments arranged by size: 3317 1388 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5457 Fragments arranged by size: 5457 683 56 BtsI GCAGTG_nn' Cuts at: 3382 3712 4506 4526 3382 Size: 330 794 20 5052 Fragments arranged by size: 5052 794 330 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6196 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2596 2631 529 Size: 35 2032 35 4094 Fragments arranged by size: 4094 2032 35 35 DraI TTT'AAA Cuts at: 3500 3500 Size: 6196 DraIII CAC_nnn'GTG Cuts at: 2777 2777 Size: 6196 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5203 809 Size: 507 3887 1802 Fragments arranged by size: 3887 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3424 3643 4532 Size: 54 1425 50 275 18 1594 219 889 Cuts at: 4532 6161 8 Size: 1629 43 Fragments arranged by size: 1629 1594 1425 889 275 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3643 1537 Size: 275 1831 4090 Fragments arranged by size: 4090 1831 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4126 1353 Size: 2773 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2026 4783 5157 5303 387 Size: 1064 138 437 2757 374 146 1280 Fragments arranged by size: 2757 1280 1064 437 374 146 138 EcoICRI GAG'CTC Cuts at: 2582 2582 Size: 6196 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2598 3141 4259 4856 5643 5911 1239 Size: 393 966 543 1118 597 787 268 1524 Fragments arranged by size: 1524 1118 966 787 597 543 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2772 2946 2772 Size: 174 6022 EcoO109I rG'GnC_Cy Cuts at: 2390 2390 Size: 6196 EcoRI G'AATT_C Cuts at: 3505 3883 3505 Size: 378 5818 FspI TGC'GCA Cuts at: 4702 4702 Size: 6196 HaeII r_GCGC'y Cuts at: 1678 3009 3331 5343 1678 Size: 1331 322 2012 2531 Fragments arranged by size: 2531 2012 1331 322 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2811 2843 3184 3216 4837 Size: 32 135 32 1239 32 341 32 1621 Cuts at: 4837 4869 4911 4943 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1621 1239 341 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2520 3540 3786 669 Size: 988 281 582 1020 246 3079 Fragments arranged by size: 3079 1020 988 582 281 246 HindIII A'AGCT_T Cuts at: 748 2935 748 Size: 2187 4009 HpaI GTT'AAC Cuts at: 2520 3786 2520 Size: 1266 4930 KpnI G_GTAC'C Cuts at: 3521 3521 Size: 6196 MfeI C'AATT_G Cuts at: 3414 3795 3414 Size: 381 5815 MluI A'CGCG_T Cuts at: 3483 6072 3483 Size: 2589 3607 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5310 5494 1305 Size: 4005 184 2007 Fragments arranged by size: 4005 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4374 Fragments arranged by size: 4374 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2186 2565 4154 4513 4672 518 Size: 648 1020 379 1589 359 159 2042 Fragments arranged by size: 2042 1589 1020 648 379 359 159 MspA1I CmG'CkG Cuts at: 1606 3612 4289 5437 5682 1606 Size: 2006 677 1148 245 2120 Fragments arranged by size: 2120 2006 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4705 Fragments arranged by size: 4705 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6196 NheI G'CTAG_C Cuts at: 1052 2177 1052 Size: 1125 5071 NotI GC'GGCC_GC Cuts at: 3643 3643 Size: 6196 NspI r_CATG'y Cuts at: 1132 1229 2536 3554 5099 1132 Size: 97 1307 1018 1545 2229 Fragments arranged by size: 2229 1545 1307 1018 97 PasI CC'CwG_GG Cuts at: 1348 2474 1348 Size: 1126 5070 PciI A'CATG_T Cuts at: 5095 5095 Size: 6196 PflMI CCAn_nnn'nTGG Cuts at: 1108 2603 2777 2780 1108 Size: 1495 174 3 4524 Fragments arranged by size: 4524 1495 174 3 PfoI T'CCnGG_A Cuts at: 2575 2575 Size: 6196 PmeI GTTT'AAAC Cuts at: 3500 3500 Size: 6196 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4265 4297 5778 5810 4265 Size: 32 1481 32 4651 Fragments arranged by size: 4651 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3064 1613 Size: 1451 4745 PsiI TTA'TAA Cuts at: 3766 3766 Size: 6196 PstI C_TGCA'G Cuts at: 830 3548 830 Size: 2718 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6196 SacI G_AGCT'C Cuts at: 2584 2584 Size: 6196 SalI G'TCGA_C Cuts at: 1936 3538 1936 Size: 1602 4594 SbfI CC_TGCA'GG Cuts at: 3548 3548 Size: 6196 ScaI AGT'ACT Cuts at: 2602 4444 6156 2602 Size: 1842 1712 2642 Fragments arranged by size: 2642 1842 1712 SexAI A'CCwGG_T Cuts at: 2773 2773 Size: 6196 SfcI C'TryA_G Cuts at: 826 1045 2631 3544 4679 5360 5551 826 Size: 219 1586 913 1135 681 191 1471 Fragments arranged by size: 1586 1471 1135 913 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6196 SmaI CCC'GGG Cuts at: 1872 3523 1872 Size: 1651 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2204 2692 2886 4300 5201 5463 Size: 197 926 261 488 194 1414 901 262 Cuts at: 5463 820 Size: 1553 Fragments arranged by size: 1553 1414 926 901 488 262 261 197 194 SnaBI TAC'GTA Cuts at: 493 3987 493 Size: 3494 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6196 SphI G_CATG'C Cuts at: 1132 2536 3554 1132 Size: 1404 1018 3774 Fragments arranged by size: 3774 1404 1018 SspI AAT'ATT Cuts at: 5 52 4120 5 Size: 47 4068 2081 Fragments arranged by size: 4068 2081 47 StuI AGG'CCT Cuts at: 1517 2280 1517 Size: 763 5433 StyI C'CwwG_G Cuts at: 513 1065 2004 3088 3635 513 Size: 552 939 1084 547 3074 Fragments arranged by size: 3074 1084 939 552 547 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2882 3478 4223 4240 4393 4578 5023 Size: 1255 1302 596 745 17 153 185 445 Cuts at: 5023 325 Size: 1498 Fragments arranged by size: 1498 1302 1255 745 596 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2600 2958 4442 Size: 274 80 33 51 1443 623 358 1484 Cuts at: 4442 6154 96 Size: 1712 138 Fragments arranged by size: 1712 1484 1443 623 358 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6156 6188 6156 Size: 32 6164 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6196 XbaI T'CTAG_A Cuts at: 3532 3532 Size: 6196 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6196 XmaI C'CCGG_G Cuts at: 1870 3521 1870 Size: 1651 4545 XmnI GAAnn'nnTTC Cuts at: 4325 4325 Size: 6196 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4004 276 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI