Restriction map for
pFN21AB3467
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspHI AspI AviII
AvrII BamHI BanII BbrPI BbsI
BbvCI BcgI BclI BcnI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsiEI BsiHKAI
BsmBI BsmI BspDI BspEI BspHI
BspMI BsrDI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HapII HgaI HindIII HpaI HpaII
KpnI KspI MamI MfeI MluI
MroI MspA1I MspI MunI NaeI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 974 GACGTC GACGT^C
AccII 1849 CGCG CG^CG
AhdI 1276 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1156 CCTAAGG CC^T[ACGT]AGG
AseI 472 ATTAAT AT^TAAT
AsnI 472 ATTAAT AT^TAAT
Asp700I 860 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1276 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 28 CTCGGG C^[CT]CG[GA]G
BalI 1878 TGGCCA TGG^CCA
BbeI 782 GGCGCC GGCGC^C
BciVI 1393 GGATAC GTATCC(6/5)
BglI 1862 GCCGCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 842 AGATCT A^GATCT
BmyI 668 GTGCCC G[AGT]GC[ACT]^C
BseRI 119 CTCCTC GAGGAG(10/8)
BsgI 1358 GTGCAG GTGCAG(16/14)
BsiWI 1591 CGTACG C^GTACG
BsmFI 1596 GGGAC GGGAC(10/14)
BsoBI 28 CTCGGG C^[CT]CG[GA]G
Bsp1286I 668 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 1575 TGTACA T^GTACA
BspLU11I 1533 ACATGT A^CATGT
BsrBI 1461 CCGCTC CCGCTC(-3/-3)
BsrGI 1575 TGTACA T^GTACA
BssSI 1178 CTCGTG CACGAG(-5/-1)
BstUI 1849 CGCG CG^CG
Bsu36I 1156 CCTAAGG CC^T[ACGT]AGG
DraII 1445 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 422 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1878 TGGCCA [CT]^GGCC[GA]
Eam1105I 1276 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1156 CCTAAGG CC^T[ACGT]AGG
EcoO109I 1445 AGGCCCT [GA]G^G[ACGT]CC[CT]
HincII 1969 GTTGAC GT[CT]^[GA]AC
HindII 1969 GTTGAC GT[CT]^[GA]AC
KasI 782 GGCGCC G^GCGCC
MluNI 1878 TGGCCA TGG^CCA
MscI 1878 TGGCCA TGG^CCA
MslI 1131 CACTATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1849 CGCG CG^CG
NarI 782 GGCGCC GG^CGCC
NcoI 1413 CCATGG C^CATGG
PshBI 472 ATTAAT AT^TAAT
PstI 692 CTGCAG CTGCA^G
SfoI 782 GGCGCC GGC^GCC
SmlI 714 CTTGAG C^T[CT][GA]AG
SphI 624 GCATGC GCATG^C
SspBI 1575 TGTACA T^GTACA
SspI 1651 AATATT AAT^ATT
XbaI 877 TCTAGA T^CTAGA
XmnI 860 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcsI 352 AAATTT [GA]^AATT[CT]
862 AAATTT
AcyI 782 GGCGCC G[GA]^CG[CT]C
974 GACGTC
AflIII 1533 ACATGT A^C[GA][CT]GT
1599 ACGTGT
ApoI 352 AAATTT [GA]^AATT[CT]
862 AAATTT
AvaII 36 GGACC G^G[AT]CC
736 GGTCC
BsaHI 782 GGCGCC G[GA]^CG[CT]C
974 GACGTC
BsaI 1345 GAGACC GGTCTC(1/5)
1418 GAGACC
HaeII 107 GGCGCT [GA]GCGC^[CT]
782 GGCGCC
Hin1I 782 GGCGCC G[GA]^CG[CT]C
974 GACGTC
HphI 331 GGTGA GGTGA(8/7)
1452 TCACC
PleI 3 GAGTC GAGTC(4/5)
717 GAGTC
TaqI 930 TCGA T^CGA
1094 TCGA
Tsp45I 332 GTGAC ^GT[GC]AC
383 GTGAC
TthHB8I 930 TCGA T^CGA
1094 TCGA
XcmI 39 CCAGCAGTTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
672 CCAAACAGGGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BanI BbvI BfaI BpmI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI HinP1I HinfI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp509I
TspRI XhoII