(Circular) (Six-base) MAPSORT of: pFN21AB3467.seq Check: 6660 from: 1 to: 6691 pFN21AB3467 6691 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2983 4501 278 Size: 53 83 186 936 1447 1518 2468 Fragments arranged by size: 2468 1518 1447 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4034 1937 Size: 2097 4594 Acc65I G'GTAC_C Cuts at: 4012 4012 Size: 6691 AclI AA'CG_TT Cuts at: 1563 3990 4818 5191 1563 Size: 2427 828 373 3063 Fragments arranged by size: 3063 2427 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2058 2242 3826 4754 6138 6406 1632 Size: 426 184 1584 928 1384 268 1917 Fragments arranged by size: 1917 1584 1384 928 426 268 184 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6494 AflIII A'CryG_T Cuts at: 1203 3538 3604 5590 6567 1203 Size: 2335 66 1986 977 1327 Fragments arranged by size: 2335 1986 1327 977 66 AgeI A'CCGG_T Cuts at: 5522 5522 Size: 6691 AhdI GACnn_n'nnGTC Cuts at: 3286 5420 3286 Size: 2134 4557 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6691 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2646 1184 Size: 1462 5229 AlwNI CAG_nnn'CTG Cuts at: 2382 2687 3178 4434 6006 2382 Size: 305 491 1256 1572 3067 Fragments arranged by size: 3067 1572 1256 491 305 ApaLI G'TGCA_C Cuts at: 4748 5904 4748 Size: 1156 5535 ApoI r'AATT_y Cuts at: 1493 2357 2867 4000 4226 4378 6487 1493 Size: 864 510 1133 226 152 2109 1697 Fragments arranged by size: 2109 1697 1133 864 510 226 152 AseI AT'TA_AT Cuts at: 160 1032 2478 5245 160 Size: 872 1446 2767 1606 Fragments arranged by size: 2767 1606 1446 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 AvaI C'yCGr_G Cuts at: 1870 1943 2033 4016 1870 Size: 73 90 1983 4545 Fragments arranged by size: 4545 1983 90 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3391 3424 3391 Size: 33 6658 BamHI G'GATC_C Cuts at: 4021 4388 4021 Size: 367 6324 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2787 2912 3797 3824 4012 Size: 325 217 627 1000 125 885 27 188 Cuts at: 4012 5467 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1000 885 627 325 217 188 125 27 BanII G_rGCy'C Cuts at: 1391 4010 1391 Size: 2619 4072 BbeI G_GCGC'C Cuts at: 2791 2791 Size: 6691 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5720 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4882 4916 1903 Size: 34 2945 34 3678 Fragments arranged by size: 3678 2945 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3392 4583 5793 3392 Size: 1191 1210 4290 Fragments arranged by size: 4290 1210 1191 BclI T'GATC_A Cuts at: 1548 1548 Size: 6691 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3873 5302 136 Size: 107 122 71 631 605 2201 1429 1525 Fragments arranged by size: 2201 1525 1429 631 605 122 107 71 BglII A'GATC_T Cuts at: 2847 6686 2847 Size: 3839 2852 BlpI GC'TnA_GC Cuts at: 4108 4108 Size: 6691 Bme1580I G_kGCm'C Cuts at: 1790 2677 4752 5908 1790 Size: 887 2075 1156 2573 Fragments arranged by size: 2573 2075 1156 887 BmgBI CAC'GTC Cuts at: 1318 3605 1318 Size: 2287 4404 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2481 4422 5380 Size: 416 53 83 399 24 1064 1941 958 Cuts at: 5380 442 Size: 1753 Fragments arranged by size: 1941 1753 1064 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6691 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2481 2601 2958 3395 5351 1239 Size: 1242 120 357 437 1956 2579 Fragments arranged by size: 2579 1956 1242 437 357 120 Bpu10I CC'TnA_GC Cuts at: 3469 3469 Size: 6691 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2740 4816 5681 5979 2740 Size: 2076 865 298 3452 Fragments arranged by size: 3452 2076 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3344 3417 5354 882 Size: 617 1845 73 1937 2219 Fragments arranged by size: 2219 1937 1845 617 73 BsaAI yAC'GTr Cuts at: 493 1204 4482 493 Size: 711 3278 2702 Fragments arranged by size: 3278 2702 711 BsaBI GATnn'nnATC Cuts at: 6685 6685 Size: 6691 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2788 2980 4498 4880 Size: 53 83 186 936 1255 192 1518 382 Cuts at: 4880 5515 275 Size: 635 1451 Fragments arranged by size: 1518 1451 1255 936 635 382 192 186 83 53 BsaWI w'CCGG_w Cuts at: 5124 5522 5796 5943 6354 5124 Size: 398 274 147 411 5461 Fragments arranged by size: 5461 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2792 2822 3100 3130 1178 Size: 30 165 30 1389 30 278 30 4739 Fragments arranged by size: 4739 1389 278 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2115 1186 Size: 929 5762 BseYI C'CCAG_C Cuts at: 5894 6390 5894 Size: 496 6195 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3384 1163 Size: 573 1648 4470 Fragments arranged by size: 4470 1648 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4141 4902 5930 1540 Size: 275 186 2140 761 1028 2301 Fragments arranged by size: 2301 2140 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4752 4837 5908 4752 Size: 85 1071 5535 Fragments arranged by size: 5535 1071 85 BsiWI C'GTAC_G Cuts at: 3596 3596 Size: 6691 BsmI GAATG_Cn' Cuts at: 4202 4295 4202 Size: 93 6598 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2677 4010 4752 4837 5908 1391 Size: 399 887 1333 742 85 1071 2174 Fragments arranged by size: 2174 1333 1071 887 742 399 85 BspEI T'CCGG_A Cuts at: 6354 6354 Size: 6691 BspHI T'CATG_A Cuts at: 4580 6310 4580 Size: 1730 4961 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4046 844 Size: 3202 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3468 4578 1394 Size: 429 1645 1110 3507 Fragments arranged by size: 3507 1645 1110 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5186 5360 65 Size: 1183 3938 174 1396 Fragments arranged by size: 3938 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5335 5522 1700 Size: 110 3525 187 2869 Fragments arranged by size: 3525 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3580 96 Size: 3484 3207 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6691 BssSI C'ACGA_G Cuts at: 3183 4751 5763 3183 Size: 1568 1012 4111 Fragments arranged by size: 4111 1568 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6691 BstBI TT'CG_AA Cuts at: 5495 5495 Size: 6691 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6691 BstYI r'GATC_y Cuts at: 1972 2222 2847 3676 4021 4388 4773 4790 6242 Size: 250 625 829 345 367 385 17 1452 Cuts at: 6242 6328 6340 6686 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 829 625 385 367 346 345 250 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3162 3162 Size: 6691 BtgI C'CryG_G Cuts at: 513 1065 2332 3418 3886 513 Size: 552 1267 1086 468 3318 Fragments arranged by size: 3318 1267 1086 552 468 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5952 Fragments arranged by size: 5952 683 56 BtsI GCAGTG_nn' Cuts at: 2025 3877 4207 5001 5021 2025 Size: 1852 330 794 20 3695 Fragments arranged by size: 3695 1852 794 330 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6691 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3471 3506 529 Size: 35 2907 35 3714 Fragments arranged by size: 3714 2907 35 35 DraI TTT'AAA Cuts at: 3995 3995 Size: 6691 DraIII CAC_nnn'GTG Cuts at: 2432 2432 Size: 6691 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5698 809 Size: 507 4382 1802 Fragments arranged by size: 4382 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3883 4138 5027 Size: 54 1425 50 275 18 2053 255 889 Cuts at: 5027 6656 8 Size: 1629 43 Fragments arranged by size: 2053 1629 1425 889 275 255 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4138 1537 Size: 275 2326 4090 Fragments arranged by size: 4090 2326 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2077 3217 3441 4621 1353 Size: 724 1140 224 1180 3423 Fragments arranged by size: 3423 1180 1140 724 224 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3119 3891 5278 5652 5798 387 Size: 1064 138 1530 772 1387 374 146 1280 Fragments arranged by size: 1530 1387 1280 1064 772 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 2242 2481 2601 2958 3395 3826 Size: 393 426 184 239 120 357 437 431 Cuts at: 3826 4754 5351 6138 6406 1239 Size: 928 597 787 268 1524 Fragments arranged by size: 1524 928 787 597 437 431 426 393 357 268 239 184 120 EcoO109I rG'GnC_Cy Cuts at: 3451 3451 Size: 6691 EcoRI G'AATT_C Cuts at: 4000 4378 4000 Size: 378 6313 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2269 3470 2269 Size: 1201 5490 FspI TGC'GCA Cuts at: 5197 5197 Size: 6691 HaeII r_GCGC'y Cuts at: 1678 2116 2791 5838 1678 Size: 438 675 3047 2531 Fragments arranged by size: 3047 2531 675 438 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2054 2086 2396 2428 2559 Size: 32 135 32 482 32 310 32 131 Cuts at: 2559 2591 3166 3198 3272 3304 5332 5364 5406 Size: 32 575 32 74 32 2028 32 42 Cuts at: 5406 5438 1373 Size: 32 2626 Fragments arranged by size: 2626 2028 575 482 310 135 131 74 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3976 4035 4281 669 Size: 988 281 2038 59 246 3079 Fragments arranged by size: 3079 2038 988 281 246 59 HindIII A'AGCT_T Cuts at: 748 748 Size: 6691 HpaI GTT'AAC Cuts at: 4281 4281 Size: 6691 KasI G'GCGC_C Cuts at: 2787 2787 Size: 6691 KpnI G_GTAC'C Cuts at: 4016 4016 Size: 6691 MfeI C'AATT_G Cuts at: 4290 4290 Size: 6691 MluI A'CGCG_T Cuts at: 6567 6567 Size: 6691 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3042 3654 5805 5989 1305 Size: 1737 612 2151 184 2007 Fragments arranged by size: 2151 2007 1737 612 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3885 10 Size: 54 1425 343 2053 2816 Fragments arranged by size: 2816 2053 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3140 4649 5008 5167 518 Size: 648 1974 1509 359 159 2042 Fragments arranged by size: 2042 1974 1509 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4107 4784 5932 6177 1606 Size: 2501 677 1148 245 2120 Fragments arranged by size: 2501 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2788 2788 Size: 6691 NcoI C'CATG_G Cuts at: 513 1065 3418 513 Size: 552 2353 3786 Fragments arranged by size: 3786 2353 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6691 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6691 NotI GC'GGCC_GC Cuts at: 4138 4138 Size: 6691 NspI r_CATG'y Cuts at: 1132 1229 2633 3408 3542 4049 5594 1132 Size: 97 1404 775 134 507 1545 2229 Fragments arranged by size: 2229 1545 1404 775 507 134 97 PasI CC'CwG_GG Cuts at: 1348 3967 1348 Size: 2619 4072 PciI A'CATG_T Cuts at: 3538 5590 3538 Size: 2052 4639 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6691 PfoI T'CCnGG_A Cuts at: 2974 2974 Size: 6691 PmeI GTTT'AAAC Cuts at: 3995 3995 Size: 6691 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2436 2468 4760 4792 6273 6305 2436 Size: 32 2292 32 1481 32 2822 Fragments arranged by size: 2822 2292 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6691 PsiI TTA'TAA Cuts at: 3960 4261 3960 Size: 301 6390 PstI C_TGCA'G Cuts at: 830 2701 4043 830 Size: 1871 1342 3478 Fragments arranged by size: 3478 1871 1342 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6691 SalI G'TCGA_C Cuts at: 1936 4033 1936 Size: 2097 4594 SbfI CC_TGCA'GG Cuts at: 4043 4043 Size: 6691 ScaI AGT'ACT Cuts at: 4939 6651 4939 Size: 1712 4979 SfcI C'TryA_G Cuts at: 826 1045 2697 3216 3495 4039 5174 5855 6046 Size: 219 1652 519 279 544 1135 681 191 Cuts at: 6046 826 Size: 1471 Fragments arranged by size: 1652 1471 1135 681 544 519 279 219 191 SfoI GGC'GCC Cuts at: 2789 2789 Size: 6691 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6691 SmaI CCC'GGG Cuts at: 1872 4018 1872 Size: 2146 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2719 4795 5696 5958 820 Size: 197 926 776 2076 901 262 1553 Fragments arranged by size: 2076 1553 926 901 776 262 197 SnaBI TAC'GTA Cuts at: 493 4482 493 Size: 3989 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6691 SphI G_CATG'C Cuts at: 1132 2633 4049 1132 Size: 1501 1416 3774 Fragments arranged by size: 3774 1501 1416 SspI AAT'ATT Cuts at: 5 52 3658 4615 5 Size: 47 3606 957 2081 Fragments arranged by size: 3606 2081 957 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6691 StyI C'CwwG_G Cuts at: 513 1065 2811 3418 3616 4130 513 Size: 552 1746 607 198 514 3074 Fragments arranged by size: 3074 1746 607 552 514 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3256 4718 4735 4888 5073 5518 325 Size: 1255 1676 1462 17 153 185 445 1498 Fragments arranged by size: 1676 1498 1462 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2096 2842 3580 Size: 274 80 33 51 1443 119 746 738 Cuts at: 3580 4937 6649 96 Size: 1357 1712 138 Fragments arranged by size: 1712 1443 1357 746 738 274 138 119 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2970 3002 6651 6683 2970 Size: 32 3649 32 2978 Fragments arranged by size: 3649 2978 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6691 XbaI T'CTAG_A Cuts at: 2882 4027 2882 Size: 1145 5546 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2051 2684 2011 Size: 40 633 6018 Fragments arranged by size: 6018 633 40 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6691 XmaI C'CCGG_G Cuts at: 1870 4016 1870 Size: 2146 4545 XmnI GAAnn'nnTTC Cuts at: 2869 4820 2869 Size: 1951 4740 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2981 4499 276 Size: 53 83 186 936 1447 1518 2468 Fragments arranged by size: 2468 1518 1447 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI