Restriction map for
pFN21AB3467
(Linear) (Six-base) MAPSORT of: KIBB3467.seq Check: 4753 from: 1 to: 1986
KIBB3467.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AatII G_ACGT'C
Cuts at: 0 978 1986
Size: 978 1008
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 237 1821 1986
Size: 53 184 1584 165
Fragments arranged by size:
1584 184 165 53
AflIII A'CryG_T
Cuts at: 0 1533 1599 1986
Size: 1533 66 387
Fragments arranged by size:
1533 387 66
AhdI GACnn_n'nnGTC
Cuts at: 0 1281 1986
Size: 1281 705
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 641 1986
Size: 641 1345
AlwNI CAG_nnn'CTG
Cuts at: 0 377 682 1173 1986
Size: 377 305 491 813
Fragments arranged by size:
813 491 377 305
ApoI r'AATT_y
Cuts at: 0 352 862 1986
Size: 352 510 1124
Fragments arranged by size:
1124 510 352
AseI AT'TA_AT
Cuts at: 0 473 1986
Size: 473 1513
AvaI C'yCGr_G
Cuts at: 0 28 1986
Size: 28 1958
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1386 1419 1986
Size: 1386 33 567
Fragments arranged by size:
1386 567 33
BanI G'GyrC_C
Cuts at: 0 782 907 1792 1819 1986
Size: 782 125 885 27 167
Fragments arranged by size:
885 782 167 125 27
BbeI G_GCGC'C
Cuts at: 0 786 1986
Size: 786 1200
BciVI GTATCCnnnnn_n'
Cuts at: 0 1387 1986
Size: 1387 599
BglI GCCn_nnn'nGGC
Cuts at: 0 1868 1986
Size: 1868 118
BglII A'GATC_T
Cuts at: 0 842 1986
Size: 842 1144
Bme1580I G_kGCm'C
Cuts at: 0 672 1986
Size: 672 1314
BmgBI CAC'GTC
Cuts at: 0 1600 1986
Size: 1600 386
BmrI ACTGGGnnnn_n'
Cuts at: 0 476 1986
Size: 476 1510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 596 953 1390 1986
Size: 476 120 357 437 596
Fragments arranged by size:
596 476 437 357 120
Bpu10I CC'TnA_GC
Cuts at: 0 1464 1986
Size: 1464 522
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 735 1986
Size: 735 1251
BsaI GGTCTCn'nnnn_
Cuts at: 0 1339 1412 1986
Size: 1339 73 574
Fragments arranged by size:
1339 574 73
BsaHI Gr'CG_yC
Cuts at: 0 783 975 1986
Size: 783 192 1011
Fragments arranged by size:
1011 783 192
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 787 817 1095 1125 1986
Size: 787 30 278 30 861
Fragments arranged by size:
861 787 278 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 110 1986
Size: 110 1876
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1379 1986
Size: 1379 607
BsiWI C'GTAC_G
Cuts at: 0 1591 1986
Size: 1591 395
Bsp1286I G_dGCh'C
Cuts at: 0 672 1986
Size: 672 1314
BsrBI CCG'CTC
Cuts at: 0 1463 1986
Size: 1463 523
BsrGI T'GTAC_A
Cuts at: 0 1575 1986
Size: 1575 411
BssSI C'ACGA_G
Cuts at: 0 1178 1986
Size: 1178 808
BstYI r'GATC_y
Cuts at: 0 217 842 1671 1986
Size: 217 625 829 315
Fragments arranged by size:
829 625 315 217
Bsu36I CC'TnA_GG
Cuts at: 0 1157 1986
Size: 1157 829
BtgI C'CryG_G
Cuts at: 0 327 1413 1881 1986
Size: 327 1086 468 105
Fragments arranged by size:
1086 468 327 105
BtsI GCAGTG_nn'
Cuts at: 0 20 1872 1986
Size: 20 1852 114
Fragments arranged by size:
1852 114 20
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1466 1501 1986
Size: 1466 35 485
Fragments arranged by size:
1466 485 35
DraIII CAC_nnn'GTG
Cuts at: 0 427 1986
Size: 427 1559
EaeI y'GGCC_r
Cuts at: 0 1878 1986
Size: 1878 108
EarI CTCTTCn'nnn_
Cuts at: 0 72 1212 1436 1986
Size: 72 1140 224 550
Fragments arranged by size:
1140 550 224 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1114 1886 1986
Size: 1114 772 100
Fragments arranged by size:
1114 772 100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 237 476 596 953 1390 1821 1986
Size: 53 184 239 120 357 437 431 165
Fragments arranged by size:
437 431 357 239 184 165 120 53
EcoO109I rG'GnC_Cy
Cuts at: 0 1446 1986
Size: 1446 540
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 264 1465 1986
Size: 264 1201 521
Fragments arranged by size:
1201 521 264
HaeII r_GCGC'y
Cuts at: 0 111 786 1986
Size: 111 675 1200
Fragments arranged by size:
1200 675 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 49 81 391 423 554 586 1161 1193
Size: 49 32 310 32 131 32 575 32
Cuts at: 1193 1267 1299 1986
Size: 74 32 687
Fragments arranged by size:
687 575 310 131 74 49 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1971 1986
Size: 1971 15
KasI G'GCGC_C
Cuts at: 0 782 1986
Size: 782 1204
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 1649 1986
Size: 1037 612 337
Fragments arranged by size:
1037 612 337
MscI TGG'CCA
Cuts at: 0 1880 1986
Size: 1880 106
MslI CAynn'nnrTG
Cuts at: 0 1135 1986
Size: 1135 851
NarI GG'CG_CC
Cuts at: 0 783 1986
Size: 783 1203
NcoI C'CATG_G
Cuts at: 0 1413 1986
Size: 1413 573
NspI r_CATG'y
Cuts at: 0 628 1403 1537 1986
Size: 628 775 134 449
Fragments arranged by size:
775 628 449 134
PasI CC'CwG_GG
Cuts at: 0 1962 1986
Size: 1962 24
PciI A'CATG_T
Cuts at: 0 1533 1986
Size: 1533 453
PfoI T'CCnGG_A
Cuts at: 0 969 1986
Size: 969 1017
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 431 463 1986
Size: 431 32 1523
Fragments arranged by size:
1523 431 32
PsiI TTA'TAA
Cuts at: 0 1955 1986
Size: 1955 31
PstI C_TGCA'G
Cuts at: 0 696 1986
Size: 696 1290
SfcI C'TryA_G
Cuts at: 0 692 1211 1490 1986
Size: 692 519 279 496
Fragments arranged by size:
692 519 496 279
SfoI GGC'GCC
Cuts at: 0 784 1986
Size: 784 1202
SmlI C'TyrA_G
Cuts at: 0 714 1986
Size: 714 1272
SphI G_CATG'C
Cuts at: 0 628 1986
Size: 628 1358
SspI AAT'ATT
Cuts at: 0 1653 1986
Size: 1653 333
StyI C'CwwG_G
Cuts at: 0 806 1413 1611 1986
Size: 806 607 198 375
Fragments arranged by size:
806 607 375 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1251 1986
Size: 1251 735
TatI w'GTAC_w
Cuts at: 0 91 837 1575 1986
Size: 91 746 738 411
Fragments arranged by size:
746 738 411 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 965 997 1986
Size: 965 32 989
Fragments arranged by size:
989 965 32
XbaI T'CTAG_A
Cuts at: 0 877 1986
Size: 877 1109
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 46 679 1986
Size: 46 633 1307
Fragments arranged by size:
1307 633 46
XmnI GAAnn'nnTTC
Cuts at: 0 864 1986
Size: 864 1122
ZraI GAC'GTC
Cuts at: 0 976 1986
Size: 976 1010
Enzymes that do cut:
AatII AcuI AflIII AhdI AlfI AlwNI ApoI AseI
AvaI BaeI BanI BbeI BciVI BglI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BsgI BsiWI Bsp1286I BsrBI BsrGI BssSI BstYI
Bsu36I BtgI BtsI CspCI DraIII EaeI EarI EciI
Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI MmeI
MscI MslI NarI NcoI NspI PasI PciI PfoI
PpiI PsiI PstI SfcI SfoI SmlI SphI SspI
StyI TaqII TatI TstI XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AleI
AloI ApaI ApaLI AscI AsiSI AvrII BamHI BanII
BbsI BbvCI BcgI BclI BfrBI BlpI BmtI BplI
BsaAI BsaBI BsaWI BseYI BsiEI BsiHKAI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrFI BssHII BstAPI BstBI
BstEII BstXI BstZ17I BtgZI ClaI DraI DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MspA1I NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PflMI PmeI PmlI
PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI
SwaI Tth111I XhoI XmaI