Restriction map for
pFN21AB3458
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# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BfrI BlnI BsaBI BsiWI BsmBI
BspHI BspLU11I BsrBI BssHII BstBI
CpoI DraI DraIII Eam1105I Eco47III
EcoT22I FseI FspI HpaI KpnI
KspI MamI MfeI MluI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SfiI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI XbaI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 3244 GACGTC GACGT^C
AccI 1071 GTATAC GT^[AC][GT]AC
AccIII 389 TCCGGA T^CCGGA
AflIII 2537 ACACGT A^C[GA][CT]GT
ApaI 3160 GGGCCC GGGCC^C
BamHI 1373 GGATCC G^GATCC
BbrPI 2538 CACGTG CAC^GTG
BbvCI 1396 CCTCAGC CCTCAGC(-5/-2)
BcgI 876 GCAACAAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 231 GGATAC GTATCC(6/5)
BglI 139 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1317 AGATCT A^GATCT
BsaI 1301 GAGACC GGTCTC(1/5)
BseAI 389 TCCGGA T^CCGGA
BsiEI 1720 CGGCCG CG[GA][CT]^CG
BsmI 111 GAATGC GAATGC(1/-1)
BspDI 2026 ATCGAT AT^CGAT
BspEI 389 TCCGGA T^CCGGA
BssSI 1858 CACGAG CACGAG(-5/-1)
Bst1107I 1071 GTATAC GTA^TAC
BstZ17I 1071 GTATAC GTA^TAC
ClaI 2026 ATCGAT AT^CGAT
EagI 1720 CGGCCG C^GGCCG
EclXI 1720 CGGCCG C^GGCCG
Eco52I 1720 CGGCCG C^GGCCG
EcoRI 467 GAATTC G^AATTC
EcoRV 2137 GATATC GAT^ATC
HincII 475 GTCAAC GT[CT]^[GA]AC
HindII 475 GTCAAC GT[CT]^[GA]AC
HindIII 400 AAGCTT A^AGCTT
MroI 389 TCCGGA T^CCGGA
PaeR7I 237 CTCGAG C^TCGAG
PmaCI 2538 CACGTG CAC^GTG
PmlI 2538 CACGTG CAC^GTG
PshAI 1323 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2757 CCTGCAGG CCTGCA^GG
SexAI 2873 ACCAGGT A^CC[AT]GGT
SmaI 1325 CCCGGG CCC^GGG
Sse8387I 2757 CCTGCAGG CCTGCA^GG
StuI 146 AGGCCT AGG^CCT
XhoI 237 CTCGAG C^TCGAG
XmaCI 1325 CCCGGG C^CCGGG
XmaI 1325 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 1820 CGCG CG^CG
1822 CGCG
AocI 142 CCTCAGG CC^T[ACGT]AGG
2777 CCTCAGG
AspI 3241 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3244 GACGTCGTC
BbsI 1221 GAAGAC GAAGAC(2/6)
3031 GTCTTC
BclI 3206 TGATCA T^GATCA
3312 TGATCA
BpmI 2215 CTCCAG CTGGAG(16/14)
2060 CTGGAG
BpuAI 1221 GAAGAC GAAGAC(2/6)
3031 GTCTTC
BsaAI 290 TACGTG [CT]AC^GT[GA]
2538 CACGTG
BsaWI 389 TCCGGA [AT]^CCGG[AT]
2573 ACCGGA
Bsp1407I 17 TGTACA T^GTACA
2484 TGTACA
BsrDI 2261 CATTGC GCAATG(2/0)
1925 GCAATG
BsrGI 17 TGTACA T^GTACA
2484 TGTACA
BstUI 1820 CGCG CG^CG
1822 CGCG
Bsu36I 142 CCTCAGG CC^T[ACGT]AGG
2777 CCTCAGG
DrdI 3238 GACGACGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3241 GACGACGTCGTC
Eco81I 142 CCTCAGG CC^T[ACGT]AGG
2777 CCTCAGG
FbaI 3206 TGATCA T^GATCA
3312 TGATCA
MseI 2371 TTAA T^TAA
2444 TTAA
MvnI 1820 CGCG CG^CG
1822 CGCG
PflFI 3241 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3244 GACGTCGTC
SapI 658 GCTCTTC GCTCTTC(1/4)
1917 GCTCTTC
ScaI 1127 AGTACT AGT^ACT
2528 AGTACT
SspBI 17 TGTACA T^GTACA
2484 TGTACA
Tru9I 2371 TTAA T^TAA
2444 TTAA
Tth111I 3241 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3244 GACGTCGTC
XcmI 30 CCATGTGGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3215 CCATCAAGGATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvI BcnI BfaI BlpI
BmyI Bpu1102I BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI