Restriction map for
pFN21AB3458
(Linear) (Six-base) MAPSORT of: KIBB3458.seq Check: 8942 from: 1 to: 3351
KIBB3458.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 597 1043 1847 3351
Size: 597 446 804 1504
Fragments arranged by size:
1504 804 597 446
AatII G_ACGT'C
Cuts at: 0 3248 3351
Size: 3248 103
AccI GT'mk_AC
Cuts at: 0 1072 3351
Size: 1072 2279
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1240 1573 1905 3302 3316 3351
Size: 646 594 333 332 1397 14 35
Fragments arranged by size:
1397 646 594 333 332 35 14
AflIII A'CryG_T
Cuts at: 0 2537 3351
Size: 2537 814
AleI CACnn'nnGTG
Cuts at: 0 442 3351
Size: 442 2909
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1535 3351
Size: 1535 1816
AlwNI CAG_nnn'CTG
Cuts at: 0 15 271 495 552 717 750 768 1410
Size: 15 256 224 57 165 33 18 642
Cuts at: 1410 2059 2766 2838 3351
Size: 649 707 72 513
Fragments arranged by size:
707 649 642 513 256 224 165 72
57 33 18 15
ApaI G_GGCC'C
Cuts at: 0 3164 3351
Size: 3164 187
ApoI r'AATT_y
Cuts at: 0 467 881 2241 2287 3351
Size: 467 414 1360 46 1064
Fragments arranged by size:
1360 1064 467 414 46
AvaI C'yCGr_G
Cuts at: 0 237 1325 1782 1828 1911 2888 3351
Size: 237 1088 457 46 83 977 463
Fragments arranged by size:
1088 977 463 457 237 83 46
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 958 991 3351
Size: 958 33 2360
Fragments arranged by size:
2360 958 33
BamHI G'GATC_C
Cuts at: 0 1373 3351
Size: 1373 1978
BanI G'GyrC_C
Cuts at: 0 327 609 689 726 1715 1786 1954 2697
Size: 327 282 80 37 989 71 168 743
Cuts at: 2697 2866 2893 2926 3171 3351
Size: 169 27 33 245 180
Fragments arranged by size:
989 743 327 282 245 180 169 168
80 71 37 33 27
BanII G_rGCy'C
Cuts at: 0 347 528 575 1117 1145 1264 1315 1461
Size: 347 181 47 542 28 119 51 146
Cuts at: 1461 1682 1897 1912 1919 2959 3164 3351
Size: 221 215 15 7 1040 205 187
Fragments arranged by size:
1040 542 347 221 215 205 187 181
146 119 51 47 28 15 7
BbeI G_GCGC'C
Cuts at: 0 1790 1958 3175 3351
Size: 1790 168 1217 176
Fragments arranged by size:
1790 1217 176 168
BbsI GAAGACnn'nnnn_
Cuts at: 0 1228 3024 3351
Size: 1228 1796 327
Fragments arranged by size:
1796 1228 327
BbvCI CC'TCA_GC
Cuts at: 0 1397 3351
Size: 1397 1954
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 865 899 3351
Size: 865 34 2452
Fragments arranged by size:
2452 865 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 225 3351
Size: 225 3126
BclI T'GATC_A
Cuts at: 0 3206 3312 3351
Size: 3206 106 39
Fragments arranged by size:
3206 106 39
BglI GCCn_nnn'nGGC
Cuts at: 0 145 3351
Size: 145 3206
BglII A'GATC_T
Cuts at: 0 1317 3351
Size: 1317 2034
BlpI GC'TnA_GC
Cuts at: 0 341 1258 1891 3351
Size: 341 917 633 1460
Fragments arranged by size:
1460 917 633 341
Bme1580I G_kGCm'C
Cuts at: 0 330 614 692 1667 2869 3164 3351
Size: 330 284 78 975 1202 295 187
Fragments arranged by size:
1202 975 330 295 284 187 78
BmrI ACTGGGnnnn_n'
Cuts at: 0 1119 1361 1412 3351
Size: 1119 242 51 1939
Fragments arranged by size:
1939 1119 242 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2081 2200 3351
Size: 2081 119 1151
Fragments arranged by size:
2081 1151 119
Bpu10I CC'TnA_GC
Cuts at: 0 722 1397 3351
Size: 722 675 1954
Fragments arranged by size:
1954 722 675
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 1669 2124 3351
Size: 158 1511 455 1227
Fragments arranged by size:
1511 1227 455 158
BsaI GGTCTCn'nnnn_
Cuts at: 0 1295 3351
Size: 1295 2056
BsaAI yAC'GTr
Cuts at: 0 292 2540 3351
Size: 292 2248 811
Fragments arranged by size:
2248 811 292
BsaHI Gr'CG_yC
Cuts at: 0 80 1787 1955 1978 1987 3172 3245 3351
Size: 80 1707 168 23 9 1185 73 106
Fragments arranged by size:
1707 1185 168 106 80 73 23 9
BsaWI w'CCGG_w
Cuts at: 0 389 2573 3351
Size: 389 2184 778
Fragments arranged by size:
2184 778 389
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 355 385 3351
Size: 355 30 2966
Fragments arranged by size:
2966 355 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1690 2650 2653 2656 2659 2662 3351
Size: 1690 960 3 3 3 3 689
Fragments arranged by size:
1690 960 689 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 576 614 1145 1190 1305 1460 2559 3351
Size: 576 38 531 45 115 155 1099 792
Fragments arranged by size:
1099 792 576 531 155 115 45 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 754 1714 3351
Size: 481 273 960 1637
Fragments arranged by size:
1637 960 481 273
BsiEI CG_ry'CG
Cuts at: 0 1723 3351
Size: 1723 1628
BsiHKAI G_wGCw'C
Cuts at: 0 540 1264 1315 2959 3351
Size: 540 724 51 1644 392
Fragments arranged by size:
1644 724 540 392 51
BsmI GAATG_Cn'
Cuts at: 0 117 3351
Size: 117 3234
Bsp1286I G_dGCh'C
Cuts at: 0 330 347 528 540 575 614 692 1117
Size: 330 17 181 12 35 39 78 425
Cuts at: 1117 1145 1264 1315 1461 1667 1682 1897 1912
Size: 28 119 51 146 206 15 215 15
Cuts at: 1912 1919 2869 2959 3164 3351
Size: 7 950 90 205 187
Fragments arranged by size:
950 425 330 215 206 205 187 181
146 119 90 78 51 39 35 28
17 15 15 12 7
BspEI T'CCGG_A
Cuts at: 0 389 3351
Size: 389 2962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 470 597 1043 1577 1847 3351
Size: 470 127 446 534 270 1504
Fragments arranged by size:
1504 534 470 446 270 127
BsrDI GCAATG_nn'
Cuts at: 0 1932 2260 3351
Size: 1932 328 1091
Fragments arranged by size:
1932 1091 328
BsrFI r'CCGG_y
Cuts at: 0 582 1718 2404 3351
Size: 582 1136 686 947
Fragments arranged by size:
1136 947 686 582
BsrGI T'GTAC_A
Cuts at: 0 17 2484 3351
Size: 17 2467 867
Fragments arranged by size:
2467 867 17
BssSI C'ACGA_G
Cuts at: 0 1858 3351
Size: 1858 1493
BstAPI GCAn_nnn'nTGC
Cuts at: 0 789 1005 1029 3351
Size: 789 216 24 2322
Fragments arranged by size:
2322 789 216 24
BstEII G'GTnAC_C
Cuts at: 0 4 2046 2877 3351
Size: 4 2042 831 474
Fragments arranged by size:
2042 831 474 4
BstXI CCAn_nnnn'nTGG
Cuts at: 0 33 106 292 1951 2511 3351
Size: 33 73 186 1659 560 840
Fragments arranged by size:
1659 840 560 186 73 33
BstYI r'GATC_y
Cuts at: 0 386 1317 1373 3351
Size: 386 931 56 1978
Fragments arranged by size:
1978 931 386 56
BstZ17I GTA'TAC
Cuts at: 0 1073 3351
Size: 1073 2278
Bsu36I CC'TnA_GG
Cuts at: 0 143 2778 3351
Size: 143 2635 573
Fragments arranged by size:
2635 573 143
BtgI C'CryG_G
Cuts at: 0 39 566 1266 1599 1944 1996 2741 2859
Size: 39 527 700 333 345 52 745 118
Cuts at: 2859 3164 3351
Size: 305 187
Fragments arranged by size:
745 700 527 345 333 305 187 118
52 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1333 3351
Size: 1333 2018
BtsI GCAGTG_nn'
Cuts at: 0 518 748 3351
Size: 518 230 2603
Fragments arranged by size:
2603 518 230
ClaI AT'CG_AT
Cuts at: 0 2027 3351
Size: 2027 1324
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3003 3038 3351
Size: 3003 35 313
Fragments arranged by size:
3003 313 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 3244 3247 3351
Size: 3244 3 104
Fragments arranged by size:
3244 104 3
EaeI y'GGCC_r
Cuts at: 0 222 302 1485 1584 1720 1941 2191 2844
Size: 222 80 1183 99 136 221 250 653
Cuts at: 2844 3351
Size: 507
Fragments arranged by size:
1183 653 507 250 222 221 136 99
80
EagI C'GGCC_G
Cuts at: 0 1720 3351
Size: 1720 1631
EarI CTCTTCn'nnn_
Cuts at: 0 665 1738 1924 2621 2681 3269 3351
Size: 665 1073 186 697 60 588 82
Fragments arranged by size:
1073 697 665 588 186 82 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 143 532 2580 3351
Size: 143 389 2048 771
Fragments arranged by size:
2048 771 389 143
EcoICRI GAG'CTC
Cuts at: 0 1262 1313 2957 3351
Size: 1262 51 1644 394
Fragments arranged by size:
1644 1262 394 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1240 1573 1905 2081 2200 3302 3316
Size: 646 594 333 332 176 119 1102 14
Cuts at: 3316 3351
Size: 35
Fragments arranged by size:
1102 646 594 333 332 176 119 35
14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 683 1289 1630 2152 3351
Size: 683 606 341 522 1199
Fragments arranged by size:
1199 683 606 522 341
EcoO109I rG'GnC_Cy
Cuts at: 0 268 756 1059 1272 1279 1393 1469 1613
Size: 268 488 303 213 7 114 76 144
Cuts at: 1613 2056 2652 2763 3160 3351
Size: 443 596 111 397 191
Fragments arranged by size:
596 488 443 397 303 268 213 191
144 114 111 76 7
EcoRI G'AATT_C
Cuts at: 0 467 3351
Size: 467 2884
EcoRV GAT'ATC
Cuts at: 0 2139 3351
Size: 2139 1212
HaeII r_GCGC'y
Cuts at: 0 1790 1958 3175 3351
Size: 1790 168 1217 176
Fragments arranged by size:
1790 1217 176 168
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1302 1334 2319 2351 2499 2531 2656 2688
Size: 1302 32 985 32 148 32 125 32
Cuts at: 2688 2882 2914 3351
Size: 194 32 437
Fragments arranged by size:
1302 985 437 194 148 125 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 477 3351
Size: 477 2874
HindIII A'AGCT_T
Cuts at: 0 400 3351
Size: 400 2951
KasI G'GCGC_C
Cuts at: 0 1786 1954 3171 3351
Size: 1786 168 1217 180
Fragments arranged by size:
1786 1217 180 168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2042 3351
Size: 2042 1309
MscI TGG'CCA
Cuts at: 0 224 304 1487 1586 2193 2846 3351
Size: 224 80 1183 99 607 653 505
Fragments arranged by size:
1183 653 607 505 224 99 80
MslI CAynn'nnrTG
Cuts at: 0 44 104 442 1294 2178 3351
Size: 44 60 338 852 884 1173
Fragments arranged by size:
1173 884 852 338 60 44
MspA1I CmG'CkG
Cuts at: 0 89 410 549 939 948 1101 1218 1410
Size: 89 321 139 390 9 153 117 192
Cuts at: 1410 1809 1968 2981 3127 3198 3351
Size: 399 159 1013 146 71 153
Fragments arranged by size:
1013 399 390 321 192 159 153 153
146 139 117 89 71 9
NarI GG'CG_CC
Cuts at: 0 1787 1955 3172 3351
Size: 1787 168 1217 179
Fragments arranged by size:
1787 1217 179 168
NcoI C'CATG_G
Cuts at: 0 39 566 1266 3351
Size: 39 527 700 2085
Fragments arranged by size:
2085 700 527 39
NspI r_CATG'y
Cuts at: 0 783 1541 1593 3351
Size: 783 758 52 1758
Fragments arranged by size:
1758 783 758 52
PasI CC'CwG_GG
Cuts at: 0 1110 2053 2766 3351
Size: 1110 943 713 585
Fragments arranged by size:
1110 943 713 585
PflMI CCAn_nnn'nTGG
Cuts at: 0 76 2059 3258 3351
Size: 76 1983 1199 93
Fragments arranged by size:
1983 1199 93 76
PfoI T'CCnGG_A
Cuts at: 0 1844 3351
Size: 1844 1507
PmlI CAC'GTG
Cuts at: 0 2540 3351
Size: 2540 811
PpuMI rG'GwC_Cy
Cuts at: 0 1059 1272 1469 2652 3351
Size: 1059 213 197 1183 699
Fragments arranged by size:
1183 1059 699 213 197
PshAI GACnn'nnGTC
Cuts at: 0 1327 3351
Size: 1327 2024
PspOMI G'GGCC_C
Cuts at: 0 3160 3351
Size: 3160 191
PspXI vC'TCGA_Gb
Cuts at: 0 237 3351
Size: 237 3114
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 43 75 3351
Size: 43 32 3276
Fragments arranged by size:
3276 43 32
PstI C_TGCA'G
Cuts at: 0 755 944 1040 2762 3351
Size: 755 189 96 1722 589
Fragments arranged by size:
1722 755 589 189 96
PvuII CAG'CTG
Cuts at: 0 549 939 1101 1218 1410 1809 1968 3127
Size: 549 390 162 117 192 399 159 1159
Cuts at: 3127 3198 3351
Size: 71 153
Fragments arranged by size:
1159 549 399 390 192 162 159 153
117 71
SacI G_AGCT'C
Cuts at: 0 1264 1315 2959 3351
Size: 1264 51 1644 392
Fragments arranged by size:
1644 1264 392 51
SanDI GG'GwC_CC
Cuts at: 0 1059 1469 3351
Size: 1059 410 1882
Fragments arranged by size:
1882 1059 410
SapI GCTCTTCn'nnn_
Cuts at: 0 665 1924 3351
Size: 665 1259 1427
Fragments arranged by size:
1427 1259 665
SbfI CC_TGCA'GG
Cuts at: 0 2762 3351
Size: 2762 589
ScaI AGT'ACT
Cuts at: 0 1129 2530 3351
Size: 1129 1401 821
Fragments arranged by size:
1401 1129 821
SexAI A'CCwGG_T
Cuts at: 0 2873 3351
Size: 2873 478
SfcI C'TryA_G
Cuts at: 0 751 934 940 1036 1155 2072 2758 3351
Size: 751 183 6 96 119 917 686 593
Fragments arranged by size:
917 751 686 593 183 119 96 6
SfoI GGC'GCC
Cuts at: 0 1788 1956 3173 3351
Size: 1788 168 1217 178
Fragments arranged by size:
1788 1217 178 168
SmaI CCC'GGG
Cuts at: 0 1327 3351
Size: 1327 2024
SmlI C'TyrA_G
Cuts at: 0 173 237 1684 2103 3351
Size: 173 64 1447 419 1248
Fragments arranged by size:
1447 1248 419 173 64
StuI AGG'CCT
Cuts at: 0 148 3351
Size: 148 3203
StyI C'CwwG_G
Cuts at: 0 39 219 566 684 1266 2882 3351
Size: 39 180 347 118 582 1616 469
Fragments arranged by size:
1616 582 469 347 180 118 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 454 1870 1899 2944 3351
Size: 454 1416 29 1045 407
Fragments arranged by size:
1416 1045 454 407 29
TatI w'GTAC_w
Cuts at: 0 17 1127 2484 2528 3351
Size: 17 1110 1357 44 823
Fragments arranged by size:
1357 1110 823 44 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 65 97 572 604 1079 1111 1579 1611
Size: 65 32 475 32 475 32 468 32
Cuts at: 1611 2708 2740 3351
Size: 1097 32 611
Fragments arranged by size:
1097 611 475 475 468 65 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3244 3247 3351
Size: 3244 3 104
Fragments arranged by size:
3244 104 3
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 37 3222 3351
Size: 37 3185 129
Fragments arranged by size:
3185 129 37
XhoI C'TCGA_G
Cuts at: 0 237 3351
Size: 237 3114
XmaI C'CCGG_G
Cuts at: 0 1325 3351
Size: 1325 2026
XmnI GAAnn'nnTTC
Cuts at: 0 55 1558 1708 3351
Size: 55 1503 150 1643
Fragments arranged by size:
1643 1503 150 55
ZraI GAC'GTC
Cuts at: 0 3246 3351
Size: 3246 105
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AlwNI
ApaI ApoI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII
HindIII KasI MmeI MscI MslI MspA1I NarI NcoI
NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuII SacI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AloI ApaLI
AscI AseI AsiSI AvrII BfrBI BmgBI BmtI BplI
BsaBI BsiWI BsmBI BspHI BsrBI BssHII BstBI DraI
DraIII FalI FseI FspI FspAI HpaI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PpiI PsiI PvuI RsrII SacII
SalI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XbaI