Restriction map for
pFN21AB3404
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AcyI AgeI Alw44I
Aor51HI ApaLI AscI Asp718I AvaI
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BcnI BlnI
BlpI Bpu1102I BsaHI BsgI BsiEI
BsiWI BsmBI BsoBI BspDI BsrDI
BsrFI BssHII BstEII BstPI CelII
Cfr10I ClaI CpoI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoT22I
FseI Hin1I KasI KpnI MluI
NaeI NarI NciI NgoMIV NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SalI SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 2381 GTATAC GT^[AC][GT]AC
AccII 5056 CGCG CG^CG
AccIII 4891 TCCGGA T^CCGGA
AclI 1614 AACGTT AA^CGTT
AflIII 3633 ACATGT A^C[GA][CT]GT
AocI 4678 CCTCAGG CC^T[ACGT]AGG
ApaI 49 GGGCCC GGGCC^C
AspI 983 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 3705 TGATCA T^GATCA
BglI 2674 GCCAAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1943 TACGTG [CT]AC^GT[GA]
BsaBI 3969 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4891 TCCGGA [AT]^CCGG[AT]
BseAI 4891 TCCGGA T^CCGGA
BspEI 4891 TCCGGA T^CCGGA
BspLU11I 3633 ACATGT A^CATGT
BspMI 3885 GCAGGT ACCTGC(4/8)
Bst1107I 2381 GTATAC GTA^TAC
BstBI 953 TTCGAA TT^CGAA
BstUI 5056 CGCG CG^CG
BstXI 1265 CCACCTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2381 GTATAC GTA^TAC
Bsu36I 4678 CCTCAGG CC^T[ACGT]AGG
Eco81I 4678 CCTCAGG CC^T[ACGT]AGG
EcoRV 4288 GATATC GAT^ATC
FbaI 3705 TGATCA T^GATCA
HaeII 1346 AGCGCC [GA]GCGC^[CT]
HapII 4892 CCGG C^CGG
HpaI 2313 GTTAAC GTT^AAC
HpaII 4892 CCGG C^CGG
KspI 5055 CCGCGG CCGC^GG
MamI 3969 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 563 CAATTG C^AATTG
MroI 4891 TCCGGA T^CCGGA
MspI 4892 CCGG C^CGG
MunI 563 CAATTG C^AATTG
MvnI 5056 CGCG CG^CG
NdeI 3128 CATATG CA^TATG
NspV 953 TTCGAA TT^CGAA
PflFI 983 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1614 AACGTT AA^CGTT
PstI 1414 CTGCAG CTGCA^G
SacI 3851 GAGCTC GAGCT^C
SacII 5055 CCGCGG CCGC^GG
SapI 2806 GAAGAGC GCTCTTC(1/4)
SfuI 953 TTCGAA TT^CGAA
StuI 2516 AGGCCT AGG^CCT
Tth111I 983 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1880 CCAAAAAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AhdI 790 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3081 GACAAAAGGTC
AseI 4398 ATTAAT AT^TAAT
4584 ATTAAT
AsnI 4398 ATTAAT AT^TAAT
4584 ATTAAT
AspEI 790 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3081 GACAAAAGGTC
AviII 1862 TGCGCA TGC^GCA
4117 TGCGCA
BbsI 223 GAAGAC GAAGAC(2/6)
1003 GAAGAC
BpuAI 223 GAAGAC GAAGAC(2/6)
1003 GAAGAC
BseRI 254 GAGGAG GAGGAG(10/8)
4545 GAGGAG
BspHI 881 TCATGA T^CATGA
3588 TCATGA
BssSI 2501 CACGAG CACGAG(-5/-1)
2576 CACGAG
BstAPI 1854 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4854 GCAGCCCATGC
Eam1105I 790 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3081 GACAAAAGGTC
EcoNI 3106 CCTCAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4741 CCTGAGCCAGG
EcoRI 1732 GAATTC G^AATTC
4822 GAATTC
FspI 1862 TGCGCA TGC^GCA
4117 TGCGCA
HgaI 1699 GACGC GACGC(5/10)
2604 GCGTC
HincII 1392 GTCAAC GT[CT]^[GA]AC
2313 GTTAAC
HindII 1392 GTCAAC GT[CT]^[GA]AC
2313 GTTAAC
MaeII 1615 ACGT A^CGT
1944 ACGT
NheI 3420 GCTAGC G^CTAGC
4765 GCTAGC
PshBI 4398 ATTAAT AT^TAAT
4584 ATTAAT
PvuII 2788 CAGCTG CAG^CTG
3158 CAGCTG
RcaI 881 TCATGA T^CATGA
3588 TCATGA
SexAI 452 ACCTGGT A^CC[AT]GGT
1823 ACCTGGT
SpeI 1213 ACTAGT A^CTAGT
1650 ACTAGT
XbaI 1302 TCTAGA T^CTAGA
4198 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanII BbvI BfaI
BfrI BglII BmyI BpmI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BsrBI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DrdI DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HindIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI