(Circular) (Six-base) MAPSORT of: pFN21AB3404.seq Check: 1565 from: 1 to: 9778 pFN21AB3404 9778 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7588 278 Size: 53 83 186 936 6052 2468 Fragments arranged by size: 6052 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4387 7121 1937 Size: 2450 2734 4594 Fragments arranged by size: 4594 2734 2450 Acc65I G'GTAC_C Cuts at: 7099 7099 Size: 9778 AclI AA'CG_TT Cuts at: 1563 3620 7905 8278 1563 Size: 2057 4285 373 3063 Fragments arranged by size: 4285 3063 2057 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2065 2547 2696 3348 3945 4022 4344 4502 Size: 433 482 149 652 597 77 322 158 Cuts at: 4502 4563 4830 5259 5968 6502 7841 9225 9493 Size: 61 267 429 709 534 1339 1384 268 Cuts at: 9493 1632 Size: 1917 Fragments arranged by size: 1917 1384 1339 709 652 597 534 482 433 429 322 268 267 158 149 77 61 AflII C'TTAA_G Cuts at: 820 1017 3853 4157 4381 820 Size: 197 2836 304 224 6217 Fragments arranged by size: 6217 2836 304 224 197 AflIII A'CryG_T Cuts at: 1203 5638 8677 9654 1203 Size: 4435 3039 977 1327 Fragments arranged by size: 4435 3039 1327 977 AgeI A'CCGG_T Cuts at: 8609 8609 Size: 9778 AhdI GACnn_n'nnGTC Cuts at: 2800 5091 8507 2800 Size: 2291 3416 4071 Fragments arranged by size: 4071 3416 2291 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9778 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9778 AlwNI CAG_nnn'CTG Cuts at: 2714 5435 6655 7521 9093 2714 Size: 2721 1220 866 1572 3399 Fragments arranged by size: 3399 2721 1572 1220 866 ApaI G_GGCC'C Cuts at: 2058 2058 Size: 9778 ApaLI G'TGCA_C Cuts at: 7835 8991 7835 Size: 1156 8622 ApoI r'AATT_y Cuts at: 1493 2507 3112 3142 3737 4271 4564 5300 5501 Size: 1014 605 30 595 534 293 736 201 Cuts at: 5501 5936 5946 6827 7087 7313 7465 9574 1493 Size: 435 10 881 260 226 152 2109 1697 Fragments arranged by size: 2109 1697 1014 881 736 605 595 534 435 293 260 226 201 152 30 10 AseI AT'TA_AT Cuts at: 160 1032 6404 6590 8332 160 Size: 872 5372 186 1742 1606 Fragments arranged by size: 5372 1742 1606 872 186 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9778 AvaI C'yCGr_G Cuts at: 1870 1943 7103 1870 Size: 73 5160 4545 Fragments arranged by size: 5160 4545 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4496 4529 4496 Size: 33 9745 BamHI G'GATC_C Cuts at: 7108 7475 7108 Size: 367 9411 BanI G'GyrC_C Cuts at: 618 943 1160 1787 7099 8554 618 Size: 325 217 627 5312 1455 1842 Fragments arranged by size: 5312 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2058 5860 6887 7097 1391 Size: 667 3802 1027 210 4072 Fragments arranged by size: 4072 3802 1027 667 210 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2235 3015 928 Size: 971 336 780 7691 Fragments arranged by size: 7691 971 780 336 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7969 8003 1903 Size: 34 6032 34 3678 Fragments arranged by size: 6032 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7670 8880 7670 Size: 1210 8568 BclI T'GATC_A Cuts at: 1548 5710 1548 Size: 4162 5616 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4685 8389 136 Size: 107 122 71 631 605 3013 3704 1525 Fragments arranged by size: 3704 3013 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3640 4420 4820 6132 6200 6661 9773 3640 Size: 780 400 1312 68 461 3112 3645 Fragments arranged by size: 3645 3112 1312 780 461 400 68 BlpI GC'TnA_GC Cuts at: 7195 7195 Size: 9778 Bme1580I G_kGCm'C Cuts at: 1790 2058 7839 8995 1790 Size: 268 5781 1156 2573 Fragments arranged by size: 5781 2573 1156 268 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9778 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5253 7007 7509 Size: 416 53 83 399 24 3836 1754 502 Cuts at: 7509 8467 442 Size: 958 1753 Fragments arranged by size: 3836 1754 1753 958 502 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5429 6774 1056 Size: 4373 1345 4060 Fragments arranged by size: 4373 4060 1345 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2031 2709 4664 5187 6121 6657 6855 7067 Size: 792 678 1955 523 934 536 198 212 Cuts at: 7067 8438 1239 Size: 1371 2579 Fragments arranged by size: 2579 1955 1371 934 792 678 536 523 212 198 Bpu10I CC'TnA_GC Cuts at: 2946 4525 5157 6747 6995 2946 Size: 1579 632 1590 248 5729 Fragments arranged by size: 5729 1590 1579 632 248 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3377 7903 8768 9066 3377 Size: 4526 865 298 4089 Fragments arranged by size: 4526 4089 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5502 5780 6386 8441 882 Size: 617 4003 278 606 2055 2219 Fragments arranged by size: 4003 2219 2055 617 606 278 BsaAI yAC'GTr Cuts at: 493 1204 3950 7569 493 Size: 711 2746 3619 2702 Fragments arranged by size: 3619 2746 2702 711 BsaBI GATnn'nnATC Cuts at: 5978 9772 5978 Size: 3794 5984 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7585 7967 8602 275 Size: 53 83 186 936 6052 382 635 1451 Fragments arranged by size: 6052 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6896 8211 8609 8883 9030 9441 6896 Size: 1315 398 274 147 411 7233 Fragments arranged by size: 7233 1315 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4637 4667 1178 Size: 30 165 30 3234 30 6289 Fragments arranged by size: 6289 3234 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2274 6565 1186 Size: 1088 4291 4399 Fragments arranged by size: 4399 4291 1088 BseYI C'CCAG_C Cuts at: 5385 6925 7068 8981 9477 5385 Size: 1540 143 1913 496 5686 Fragments arranged by size: 5686 1913 1540 496 143 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 9205 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7228 7989 9017 1540 Size: 275 186 5227 761 1028 2301 Fragments arranged by size: 5227 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3966 4842 5194 5860 7055 7839 7924 8995 3966 Size: 876 352 666 1195 784 85 1071 4749 Fragments arranged by size: 4749 1195 1071 876 784 666 352 85 BsmI GAATG_Cn' Cuts at: 2132 4855 6967 7289 7382 2132 Size: 2723 2112 322 93 4528 Fragments arranged by size: 4528 2723 2112 322 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 3966 4842 5194 5860 6887 7055 Size: 399 268 1908 876 352 666 1027 168 Cuts at: 7055 7097 7839 7924 8995 1391 Size: 42 742 85 1071 2174 Fragments arranged by size: 2174 1908 1071 1027 876 742 666 399 352 268 168 85 42 BspEI T'CCGG_A Cuts at: 6896 9441 6896 Size: 2545 7233 BspHI T'CATG_A Cuts at: 2886 5593 7667 9397 2886 Size: 2707 2074 1730 3267 Fragments arranged by size: 3267 2707 2074 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5881 7133 844 Size: 5037 1252 3489 Fragments arranged by size: 5037 3489 1252 BsrBI CCG'CTC Cuts at: 1394 1823 2060 3757 5514 6759 7665 1394 Size: 429 237 1697 1757 1245 906 3507 Fragments arranged by size: 3507 1757 1697 1245 906 429 237 BsrDI GCAATG_nn' Cuts at: 65 1248 8273 8447 65 Size: 1183 7025 174 1396 Fragments arranged by size: 7025 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 8422 8609 1700 Size: 110 6612 187 2869 Fragments arranged by size: 6612 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2596 5635 6273 96 Size: 2500 3039 638 3601 Fragments arranged by size: 3601 3039 2500 638 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9778 BssSI C'ACGA_G Cuts at: 4506 4581 7838 8850 4506 Size: 75 3257 1012 5434 Fragments arranged by size: 5434 3257 1012 75 BstAPI GCAn_nnn'nTGC Cuts at: 794 3865 6865 794 Size: 3071 3000 3707 Fragments arranged by size: 3707 3071 3000 BstBI TT'CG_AA Cuts at: 2959 8582 2959 Size: 5623 4155 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9778 BstXI CCAn_nnnn'nTGG Cuts at: 3277 3277 Size: 9778 BstYI r'GATC_y Cuts at: 1972 2950 3280 3640 4420 4820 5107 6132 6200 Size: 978 330 360 780 400 287 1025 68 Cuts at: 6200 6568 6651 6661 7108 7475 7860 7877 9329 Size: 368 83 10 447 367 385 17 1452 Cuts at: 9329 9415 9427 9773 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1025 978 780 447 400 385 368 367 360 346 330 287 86 83 68 17 12 10 BstZ17I GTA'TAC Cuts at: 4388 4388 Size: 9778 Bsu36I CC'TnA_GG Cuts at: 6684 6684 Size: 9778 BtgI C'CryG_G Cuts at: 513 1065 2757 5851 6312 7060 513 Size: 552 1692 3094 461 748 3231 Fragments arranged by size: 3231 3094 1692 748 552 461 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4506 487 Size: 683 56 3280 5759 Fragments arranged by size: 5759 3280 683 56 BtsI GCAGTG_nn' Cuts at: 2081 6781 7294 8088 8108 2081 Size: 4700 513 794 20 3751 Fragments arranged by size: 4700 3751 794 513 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9778 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9743 DraI TTT'AAA Cuts at: 3138 4221 5941 7082 3138 Size: 1083 1720 1141 5834 Fragments arranged by size: 5834 1720 1141 1083 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2932 5516 5742 8785 809 Size: 507 1616 2584 226 3043 1802 Fragments arranged by size: 3043 2584 1802 1616 507 226 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3184 3726 6224 Size: 54 1425 50 275 18 1354 542 2498 Cuts at: 6224 7225 8114 9743 8 Size: 1001 889 1629 43 Fragments arranged by size: 2498 1629 1425 1354 1001 889 542 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7225 1537 Size: 275 5413 4090 Fragments arranged by size: 5413 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2143 3813 4240 4404 4692 4806 6336 6525 Size: 790 1670 427 164 288 114 1530 189 Cuts at: 6525 7708 1353 Size: 1183 3423 Fragments arranged by size: 3423 1670 1530 1183 790 427 288 189 164 114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8365 8739 8885 387 Size: 1064 138 6776 374 146 1280 Fragments arranged by size: 6776 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5858 5858 Size: 9778 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2031 2065 2547 2696 2709 3348 3945 Size: 393 399 34 482 149 13 639 597 Cuts at: 3945 4022 4344 4502 4563 4664 4830 5187 5259 Size: 77 322 158 61 101 166 357 72 Cuts at: 5259 5968 6121 6502 6657 6855 7067 7841 8438 Size: 709 153 381 155 198 212 774 597 Cuts at: 8438 9225 9493 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 774 709 639 597 597 482 399 393 381 357 322 268 212 198 166 158 155 153 149 101 77 72 61 34 13 EcoNI CCTnn'n_nnAGG Cuts at: 5115 6750 5115 Size: 1635 8143 EcoO109I rG'GnC_Cy Cuts at: 5093 5893 6632 7072 5093 Size: 800 739 440 7799 Fragments arranged by size: 7799 800 739 440 EcoRI G'AATT_C Cuts at: 3737 6827 7087 7465 3737 Size: 3090 260 378 6050 Fragments arranged by size: 6050 3090 378 260 EcoRV GAT'ATC Cuts at: 6295 6295 Size: 9778 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3386 4783 5622 3386 Size: 1397 839 7542 Fragments arranged by size: 7542 1397 839 FspI TGC'GCA Cuts at: 3869 6124 8284 3869 Size: 2255 2160 5363 Fragments arranged by size: 5363 2255 2160 HaeII r_GCGC'y Cuts at: 1678 3355 8925 1678 Size: 1677 5570 2531 Fragments arranged by size: 5570 2531 1677 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2720 2752 2786 2818 4637 Size: 32 135 32 1148 32 34 32 1819 Cuts at: 4637 4669 4805 4837 5077 5109 6293 6313 6319 Size: 32 136 32 240 32 1184 20 6 Cuts at: 6319 6325 6345 6351 6941 6973 8419 8451 8493 Size: 6 20 6 590 32 1446 32 42 Cuts at: 8493 8525 1373 Size: 32 2626 Fragments arranged by size: 2626 1819 1446 1184 1148 590 240 136 135 42 34 32 32 32 32 32 32 32 32 32 32 20 20 6 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 3399 4320 7122 7368 669 Size: 988 281 1461 921 2802 246 3079 Fragments arranged by size: 3079 2802 1461 988 921 281 246 HindIII A'AGCT_T Cuts at: 748 3330 3850 4358 748 Size: 2582 520 508 6168 Fragments arranged by size: 6168 2582 520 508 HpaI GTT'AAC Cuts at: 4320 7368 4320 Size: 3048 6730 KpnI G_GTAC'C Cuts at: 7103 7103 Size: 9778 MfeI C'AATT_G Cuts at: 2568 7377 2568 Size: 4809 4969 MluI A'CGCG_T Cuts at: 9654 9654 Size: 9778 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2013 5081 5327 6369 6452 6626 6674 7005 Size: 708 3068 246 1042 83 174 48 331 Cuts at: 7005 7014 8892 9076 1305 Size: 9 1878 184 2007 Fragments arranged by size: 3068 2007 1878 1042 708 331 246 184 174 83 48 9 MscI TGG'CCA Cuts at: 10 64 1489 1832 3186 3728 6226 10 Size: 54 1425 343 1354 542 2498 3562 Fragments arranged by size: 3562 2498 1425 1354 542 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4140 5869 6072 7736 8095 8254 518 Size: 648 2974 1729 203 1664 359 159 2042 Fragments arranged by size: 2974 2042 1729 1664 648 359 203 159 MspA1I CmG'CkG Cuts at: 1606 4795 5165 7062 7194 7871 9019 9264 1606 Size: 3189 370 1897 132 677 1148 245 2120 Fragments arranged by size: 3189 2120 1897 1148 677 370 245 132 NcoI C'CATG_G Cuts at: 513 1065 2757 5851 6312 513 Size: 552 1692 3094 461 3979 Fragments arranged by size: 3979 3094 1692 552 461 NdeI CA'TA_TG Cuts at: 387 5134 387 Size: 4747 5031 NheI G'CTAG_C Cuts at: 1052 5425 6770 1052 Size: 4373 1345 4060 Fragments arranged by size: 4373 4060 1345 NotI GC'GGCC_GC Cuts at: 7225 7225 Size: 9778 NspI r_CATG'y Cuts at: 1132 1229 2367 4139 5642 5868 7136 8681 1132 Size: 97 1138 1772 1503 226 1268 1545 2229 Fragments arranged by size: 2229 1772 1545 1503 1268 1138 226 97 PasI CC'CwG_GG Cuts at: 1348 5098 1348 Size: 3750 6028 PciI A'CATG_T Cuts at: 5638 8677 5638 Size: 3039 6739 PflMI CCAn_nnn'nTGG Cuts at: 1108 3203 5974 6318 1108 Size: 2095 2771 344 4568 Fragments arranged by size: 4568 2771 2095 344 PfoI T'CCnGG_A Cuts at: 2436 6634 2436 Size: 4198 5580 PmeI GTTT'AAAC Cuts at: 7082 7082 Size: 9778 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7847 7879 9360 9392 7847 Size: 32 1481 32 8233 Fragments arranged by size: 8233 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5093 5893 6632 7072 5093 Size: 800 739 440 7799 Fragments arranged by size: 7799 800 739 440 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9778 PsiI TTA'TAA Cuts at: 7348 7348 Size: 9778 PspOMI G'GGCC_C Cuts at: 2054 2054 Size: 9778 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 6432 6464 6432 Size: 32 9746 PstI C_TGCA'G Cuts at: 830 3423 7130 830 Size: 2593 3707 3478 Fragments arranged by size: 3707 3478 2593 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9778 PvuII CAG'CTG Cuts at: 4795 5165 4795 Size: 370 9408 SacI G_AGCT'C Cuts at: 5860 5860 Size: 9778 SacII CC_GC'GG Cuts at: 7063 7063 Size: 9778 SalI G'TCGA_C Cuts at: 1936 7120 1936 Size: 5184 4594 SanDI GG'GwC_CC Cuts at: 7072 7072 Size: 9778 SapI GCTCTTCn'nnn_ Cuts at: 4806 4806 Size: 9778 SbfI CC_TGCA'GG Cuts at: 7130 7130 Size: 9778 ScaI AGT'ACT Cuts at: 8026 9738 8026 Size: 1712 8066 SexAI A'CCwGG_T Cuts at: 2457 3828 2457 Size: 1371 8407 SfcI C'TryA_G Cuts at: 826 1045 2280 2715 3419 5199 6844 7126 8261 Size: 219 1235 435 704 1780 1645 282 1135 Cuts at: 8261 8942 9133 826 Size: 681 191 1471 Fragments arranged by size: 1780 1645 1471 1235 1135 704 681 435 282 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9778 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9778 SmaI CCC'GGG Cuts at: 1872 7105 1872 Size: 5233 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3356 3853 4157 4381 7882 8783 Size: 197 926 1413 497 304 224 3501 901 Cuts at: 8783 9045 820 Size: 262 1553 Fragments arranged by size: 3501 1553 1413 926 901 497 304 262 224 197 SnaBI TAC'GTA Cuts at: 493 7569 493 Size: 7076 2702 SpeI A'CTAG_T Cuts at: 152 3218 3655 152 Size: 3066 437 6275 Fragments arranged by size: 6275 3066 437 SphI G_CATG'C Cuts at: 1132 7136 1132 Size: 6004 3774 SspI AAT'ATT Cuts at: 5 52 2241 2325 3055 7702 5 Size: 47 2189 84 730 4647 2081 Fragments arranged by size: 4647 2189 2081 730 84 47 StuI AGG'CCT Cuts at: 1517 4523 1517 Size: 3006 6772 StyI C'CwwG_G Cuts at: 513 1065 2757 5851 6312 6910 7217 513 Size: 552 1692 3094 461 598 307 3074 Fragments arranged by size: 3094 3074 1692 598 552 461 307 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3039 3791 5446 5804 7805 7822 7975 Size: 1255 1459 752 1655 358 2001 17 153 Cuts at: 7975 8160 8605 325 Size: 185 445 1498 Fragments arranged by size: 2001 1655 1498 1459 1255 752 445 358 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2596 3229 5635 Size: 274 80 33 51 1443 619 633 2406 Cuts at: 5635 6273 8024 9736 96 Size: 638 1751 1712 138 Fragments arranged by size: 2406 1751 1712 1443 638 633 619 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3393 3425 3934 3966 6206 6238 9738 9770 3393 Size: 32 509 32 2240 32 3500 32 3401 Fragments arranged by size: 3500 3401 2240 509 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2991 1316 Size: 1675 8103 XbaI T'CTAG_A Cuts at: 3307 6203 7114 3307 Size: 2896 911 5971 Fragments arranged by size: 5971 2896 911 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3892 3892 Size: 9778 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9778 XmaI C'CCGG_G Cuts at: 1870 7103 1870 Size: 5233 4545 XmnI GAAnn'nnTTC Cuts at: 2748 4476 5700 5910 6321 7907 2748 Size: 1728 1224 210 411 1586 4619 Fragments arranged by size: 4619 1728 1586 1224 411 210 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7586 276 Size: 53 83 186 936 6052 2468 Fragments arranged by size: 6052 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SfiI SfoI SrfI SwaI