Restriction map for
pFN21AB3404
(Linear) (Six-base) MAPSORT of: KIBB3404.seq Check: 1141 from: 1 to: 5073
KIBB3404.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AccI GT'mk_AC
Cuts at: 0 2382 5073
Size: 2382 2691
AclI AA'CG_TT
Cuts at: 0 1615 5073
Size: 1615 3458
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 542 691 1343 1940 2017 2339 2497
Size: 60 482 149 652 597 77 322 158
Cuts at: 2497 2558 2825 3254 3963 4497 5073
Size: 61 267 429 709 534 576
Fragments arranged by size:
709 652 597 576 534 482 429 322
267 158 149 77 61 60
AflII C'TTAA_G
Cuts at: 0 1848 2152 2376 5073
Size: 1848 304 224 2697
Fragments arranged by size:
2697 1848 304 224
AflIII A'CryG_T
Cuts at: 0 3633 5073
Size: 3633 1440
AhdI GACnn_n'nnGTC
Cuts at: 0 795 3086 5073
Size: 795 2291 1987
Fragments arranged by size:
2291 1987 795
AlwNI CAG_nnn'CTG
Cuts at: 0 709 3430 4650 5073
Size: 709 2721 1220 423
Fragments arranged by size:
2721 1220 709 423
ApaI G_GGCC'C
Cuts at: 0 53 5073
Size: 53 5020
ApoI r'AATT_y
Cuts at: 0 502 1107 1137 1732 2266 2559 3295 3496
Size: 502 605 30 595 534 293 736 201
Cuts at: 3496 3931 3941 4822 5073
Size: 435 10 881 251
Fragments arranged by size:
881 736 605 595 534 502 435 293
251 201 30 10
AseI AT'TA_AT
Cuts at: 0 4399 4585 5073
Size: 4399 186 488
Fragments arranged by size:
4399 488 186
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2491 2524 5073
Size: 2491 33 2549
Fragments arranged by size:
2549 2491 33
BanII G_rGCy'C
Cuts at: 0 53 3855 4882 5073
Size: 53 3802 1027 191
Fragments arranged by size:
3802 1027 191 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 230 1010 5073
Size: 230 780 4063
Fragments arranged by size:
4063 780 230
BclI T'GATC_A
Cuts at: 0 3705 5073
Size: 3705 1368
BglI GCCn_nnn'nGGC
Cuts at: 0 2680 5073
Size: 2680 2393
BglII A'GATC_T
Cuts at: 0 1635 2415 2815 4127 4195 4656 5073
Size: 1635 780 400 1312 68 461 417
Fragments arranged by size:
1635 1312 780 461 417 400 68
Bme1580I G_kGCm'C
Cuts at: 0 53 5073
Size: 53 5020
BmrI ACTGGGnnnn_n'
Cuts at: 0 3248 5002 5073
Size: 3248 1754 71
Fragments arranged by size:
3248 1754 71
BmtI G_CTAG'C
Cuts at: 0 3424 4769 5073
Size: 3424 1345 304
Fragments arranged by size:
3424 1345 304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 704 2659 3182 4116 4652 4850 5062
Size: 26 678 1955 523 934 536 198 212
Cuts at: 5062 5073
Size: 11
Fragments arranged by size:
1955 934 678 536 523 212 198 26
11
Bpu10I CC'TnA_GC
Cuts at: 0 941 2520 3152 4742 4990 5073
Size: 941 1579 632 1590 248 83
Fragments arranged by size:
1590 1579 941 632 248 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1372 5073
Size: 1372 3701
BsaI GGTCTCn'nnnn_
Cuts at: 0 3497 3775 4381 5073
Size: 3497 278 606 692
Fragments arranged by size:
3497 692 606 278
BsaAI yAC'GTr
Cuts at: 0 1945 5073
Size: 1945 3128
BsaBI GATnn'nnATC
Cuts at: 0 3973 5073
Size: 3973 1100
BsaWI w'CCGG_w
Cuts at: 0 4891 5073
Size: 4891 182
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2632 2662 5073
Size: 2632 30 2411
Fragments arranged by size:
2632 2411 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 269 4560 5073
Size: 269 4291 513
Fragments arranged by size:
4291 513 269
BseYI C'CCAG_C
Cuts at: 0 3380 4920 5063 5073
Size: 3380 1540 143 10
Fragments arranged by size:
3380 1540 143 10
BsiHKAI G_wGCw'C
Cuts at: 0 1961 2837 3189 3855 5050 5073
Size: 1961 876 352 666 1195 23
Fragments arranged by size:
1961 1195 876 666 352 23
BsmI GAATG_Cn'
Cuts at: 0 127 2850 4962 5073
Size: 127 2723 2112 111
Fragments arranged by size:
2723 2112 127 111
Bsp1286I G_dGCh'C
Cuts at: 0 53 1961 2837 3189 3855 4882 5050 5073
Size: 53 1908 876 352 666 1027 168 23
Fragments arranged by size:
1908 1027 876 666 352 168 53 23
BspEI T'CCGG_A
Cuts at: 0 4891 5073
Size: 4891 182
BspHI T'CATG_A
Cuts at: 0 881 3588 5073
Size: 881 2707 1485
Fragments arranged by size:
2707 1485 881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3876 5073
Size: 3876 1197
BsrBI CCG'CTC
Cuts at: 0 55 1752 3509 4754 5073
Size: 55 1697 1757 1245 319
Fragments arranged by size:
1757 1697 1245 319 55
BsrGI T'GTAC_A
Cuts at: 0 591 3630 4268 5073
Size: 591 3039 638 805
Fragments arranged by size:
3039 805 638 591
BssSI C'ACGA_G
Cuts at: 0 2501 2576 5073
Size: 2501 75 2497
Fragments arranged by size:
2501 2497 75
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1860 4860 5073
Size: 1860 3000 213
Fragments arranged by size:
3000 1860 213
BstBI TT'CG_AA
Cuts at: 0 954 5073
Size: 954 4119
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1272 5073
Size: 1272 3801
BstYI r'GATC_y
Cuts at: 0 945 1275 1635 2415 2815 3102 4127 4195
Size: 945 330 360 780 400 287 1025 68
Cuts at: 4195 4563 4646 4656 5073
Size: 368 83 10 417
Fragments arranged by size:
1025 945 780 417 400 368 360 330
287 83 68 10
BstZ17I GTA'TAC
Cuts at: 0 2383 5073
Size: 2383 2690
Bsu36I CC'TnA_GG
Cuts at: 0 4679 5073
Size: 4679 394
BtgI C'CryG_G
Cuts at: 0 752 3846 4307 5055 5073
Size: 752 3094 461 748 18
Fragments arranged by size:
3094 752 748 461 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2501 5073
Size: 2501 2572
BtsI GCAGTG_nn'
Cuts at: 0 76 4776 5073
Size: 76 4700 297
Fragments arranged by size:
4700 297 76
DraI TTT'AAA
Cuts at: 0 1133 2216 3936 5073
Size: 1133 1083 1720 1137
Fragments arranged by size:
1720 1137 1133 1083
DrdI GACnn_nn'nnGTC
Cuts at: 0 927 3511 3737 5073
Size: 927 2584 226 1336
Fragments arranged by size:
2584 1336 927 226
EaeI y'GGCC_r
Cuts at: 0 1179 1721 4219 5073
Size: 1179 542 2498 854
Fragments arranged by size:
2498 1179 854 542
EarI CTCTTCn'nnn_
Cuts at: 0 138 1808 2235 2399 2687 2801 4331 4520
Size: 138 1670 427 164 288 114 1530 189
Cuts at: 4520 5073
Size: 553
Fragments arranged by size:
1670 1530 553 427 288 189 164 138
114
EcoICRI GAG'CTC
Cuts at: 0 3853 5073
Size: 3853 1220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 60 542 691 704 1343 1940 2017
Size: 26 34 482 149 13 639 597 77
Cuts at: 2017 2339 2497 2558 2659 2825 3182 3254 3963
Size: 322 158 61 101 166 357 72 709
Cuts at: 3963 4116 4497 4652 4850 5062 5073
Size: 153 381 155 198 212 11
Fragments arranged by size:
709 639 597 482 381 357 322 212
198 166 158 155 153 149 101 77
72 61 34 26 13 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3110 4745 5073
Size: 3110 1635 328
Fragments arranged by size:
3110 1635 328
EcoO109I rG'GnC_Cy
Cuts at: 0 3088 3888 4627 5067 5073
Size: 3088 800 739 440 6
Fragments arranged by size:
3088 800 739 440 6
EcoRI G'AATT_C
Cuts at: 0 1732 4822 5073
Size: 1732 3090 251
Fragments arranged by size:
3090 1732 251
EcoRV GAT'ATC
Cuts at: 0 4290 5073
Size: 4290 783
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1381 2778 3617 5073
Size: 1381 1397 839 1456
Fragments arranged by size:
1456 1397 1381 839
FspI TGC'GCA
Cuts at: 0 1864 4119 5073
Size: 1864 2255 954
Fragments arranged by size:
2255 1864 954
HaeII r_GCGC'y
Cuts at: 0 1350 5073
Size: 1350 3723
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 715 747 781 813 2632 2664 2800 2832
Size: 715 32 34 32 1819 32 136 32
Cuts at: 2832 3072 3104 4288 4308 4314 4320 4340 4346
Size: 240 32 1184 20 6 6 20 6
Cuts at: 4346 4936 4968 5073
Size: 590 32 105
Fragments arranged by size:
1819 1184 715 590 240 136 105 34
32 32 32 32 32 32 20 20
6 6 6
HincII GTy'rAC
Cuts at: 0 1394 2315 5073
Size: 1394 921 2758
Fragments arranged by size:
2758 1394 921
HindIII A'AGCT_T
Cuts at: 0 1325 1845 2353 5073
Size: 1325 520 508 2720
Fragments arranged by size:
2720 1325 520 508
HpaI GTT'AAC
Cuts at: 0 2315 5073
Size: 2315 2758
MfeI C'AATT_G
Cuts at: 0 563 5073
Size: 563 4510
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 3076 3322 4364 4447 4621 4669 5000
Size: 8 3068 246 1042 83 174 48 331
Cuts at: 5000 5009 5073
Size: 9 64
Fragments arranged by size:
3068 1042 331 246 174 83 64 48
9 8
MscI TGG'CCA
Cuts at: 0 1181 1723 4221 5073
Size: 1181 542 2498 852
Fragments arranged by size:
2498 1181 852 542
MslI CAynn'nnrTG
Cuts at: 0 2135 3864 4067 5073
Size: 2135 1729 203 1006
Fragments arranged by size:
2135 1729 1006 203
MspA1I CmG'CkG
Cuts at: 0 2790 3160 5057 5073
Size: 2790 370 1897 16
Fragments arranged by size:
2790 1897 370 16
NcoI C'CATG_G
Cuts at: 0 752 3846 4307 5073
Size: 752 3094 461 766
Fragments arranged by size:
3094 766 752 461
NdeI CA'TA_TG
Cuts at: 0 3129 5073
Size: 3129 1944
NheI G'CTAG_C
Cuts at: 0 3420 4765 5073
Size: 3420 1345 308
Fragments arranged by size:
3420 1345 308
NspI r_CATG'y
Cuts at: 0 362 2134 3637 3863 5073
Size: 362 1772 1503 226 1210
Fragments arranged by size:
1772 1503 1210 362 226
PasI CC'CwG_GG
Cuts at: 0 3093 5073
Size: 3093 1980
PciI A'CATG_T
Cuts at: 0 3633 5073
Size: 3633 1440
PflMI CCAn_nnn'nTGG
Cuts at: 0 1198 3969 4313 5073
Size: 1198 2771 344 760
Fragments arranged by size:
2771 1198 760 344
PfoI T'CCnGG_A
Cuts at: 0 431 4629 5073
Size: 431 4198 444
Fragments arranged by size:
4198 444 431
PpuMI rG'GwC_Cy
Cuts at: 0 3088 3888 4627 5067 5073
Size: 3088 800 739 440 6
Fragments arranged by size:
3088 800 739 440 6
PspOMI G'GGCC_C
Cuts at: 0 49 5073
Size: 49 5024
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4427 4459 5073
Size: 4427 32 614
Fragments arranged by size:
4427 614 32
PstI C_TGCA'G
Cuts at: 0 1418 5073
Size: 1418 3655
PvuII CAG'CTG
Cuts at: 0 2790 3160 5073
Size: 2790 370 1913
Fragments arranged by size:
2790 1913 370
SacI G_AGCT'C
Cuts at: 0 3855 5073
Size: 3855 1218
SacII CC_GC'GG
Cuts at: 0 5058 5073
Size: 5058 15
SanDI GG'GwC_CC
Cuts at: 0 5067 5073
Size: 5067 6
SapI GCTCTTCn'nnn_
Cuts at: 0 2801 5073
Size: 2801 2272
SexAI A'CCwGG_T
Cuts at: 0 452 1823 5073
Size: 452 1371 3250
Fragments arranged by size:
3250 1371 452
SfcI C'TryA_G
Cuts at: 0 275 710 1414 3194 4839 5073
Size: 275 435 704 1780 1645 234
Fragments arranged by size:
1780 1645 704 435 275 234
SmlI C'TyrA_G
Cuts at: 0 1351 1848 2152 2376 5073
Size: 1351 497 304 224 2697
Fragments arranged by size:
2697 1351 497 304 224
SpeI A'CTAG_T
Cuts at: 0 1213 1650 5073
Size: 1213 437 3423
Fragments arranged by size:
3423 1213 437
SspI AAT'ATT
Cuts at: 0 236 320 1050 5073
Size: 236 84 730 4023
Fragments arranged by size:
4023 730 236 84
StuI AGG'CCT
Cuts at: 0 2518 5073
Size: 2518 2555
StyI C'CwwG_G
Cuts at: 0 752 3846 4307 4905 5073
Size: 752 3094 461 598 168
Fragments arranged by size:
3094 752 598 461 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1034 1786 3441 3799 5073
Size: 1034 752 1655 358 1274
Fragments arranged by size:
1655 1274 1034 752 358
TatI w'GTAC_w
Cuts at: 0 591 1224 3630 4268 5073
Size: 591 633 2406 638 805
Fragments arranged by size:
2406 805 638 633 591
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1388 1420 1929 1961 4201 4233 5073
Size: 1388 32 509 32 2240 32 840
Fragments arranged by size:
2240 1388 840 509 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 986 5073
Size: 986 4087
XbaI T'CTAG_A
Cuts at: 0 1302 4198 5073
Size: 1302 2896 875
Fragments arranged by size:
2896 1302 875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1887 5073
Size: 1887 3186
XmnI GAAnn'nnTTC
Cuts at: 0 743 2471 3695 3905 4316 5073
Size: 743 1728 1224 210 411 757
Fragments arranged by size:
1728 1224 757 743 411 210
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AhdI AlwNI ApaI
ApoI AseI BaeI BanII BbsI BclI BglI BglII
Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
MfeI MmeI MscI MslI MspA1I NcoI NdeI NheI
NspI PasI PciI PflMI PfoI PpuMI PspOMI PsrI
PstI PvuII SacI SacII SanDI SapI SexAI SfcI
SmlI SpeI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AleI AlfI AloI
ApaLI AscI AsiSI AvaI AvrII BamHI BanI BbeI
BbvCI BcgI BciVI BfrBI BlpI BmgBI BplI BsaHI
BsgI BsiEI BsiWI BsmBI BsrDI BsrFI BssHII BstEII
ClaI CspCI DraIII EagI EciI FseI FspAI KasI
KpnI MluI NaeI NarI NgoMIV NotI NruI NsiI
PacI PmeI PmlI PpiI PshAI PsiI PspXI PvuI
RsrII SalI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI SwaI XhoI XmaI ZraI