(Circular) (Six-base) MAPSORT of: pFN21AB3350.seq Check: 1780 from: 1 to: 6610 pFN21AB3350 6610 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2049 2049 Size: 6610 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4420 278 Size: 53 83 186 936 2884 2468 Fragments arranged by size: 2884 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3953 1937 Size: 2016 4594 Acc65I G'GTAC_C Cuts at: 3931 3931 Size: 6610 AclI AA'CG_TT Cuts at: 1563 3909 4737 5110 1563 Size: 2346 828 373 3063 Fragments arranged by size: 3063 2346 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4673 6057 6325 1632 Size: 3041 1384 268 1917 Fragments arranged by size: 3041 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6413 AflIII A'CryG_T Cuts at: 1203 3397 5509 6486 1203 Size: 2194 2112 977 1327 Fragments arranged by size: 2194 2112 1327 977 AgeI A'CCGG_T Cuts at: 5441 5441 Size: 6610 AhdI GACnn_n'nnGTC Cuts at: 5339 5339 Size: 6610 AleI CACnn'nnGTG Cuts at: 1166 3041 1166 Size: 1875 4735 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3491 1184 Size: 2307 4303 AlwNI CAG_nnn'CTG Cuts at: 2431 3283 4353 5925 2431 Size: 852 1070 1572 3116 Fragments arranged by size: 3116 1572 1070 852 ApaI G_GGCC'C Cuts at: 2361 2361 Size: 6610 ApaLI G'TGCA_C Cuts at: 3476 4667 5823 3476 Size: 1191 1156 4263 Fragments arranged by size: 4263 1191 1156 ApoI r'AATT_y Cuts at: 1493 3919 4145 4297 6406 1493 Size: 2426 226 152 2109 1697 Fragments arranged by size: 2426 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5164 160 Size: 872 4132 1606 Fragments arranged by size: 4132 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6610 AvaI C'yCGr_G Cuts at: 1870 1943 2067 2526 2690 3068 3935 1870 Size: 73 124 459 164 378 867 4545 Fragments arranged by size: 4545 867 459 378 164 124 73 BamHI G'GATC_C Cuts at: 3940 4307 3940 Size: 367 6243 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2219 3780 3931 5386 618 Size: 325 217 627 432 1561 151 1455 1842 Fragments arranged by size: 1842 1561 1455 627 432 325 217 151 BanII G_rGCy'C Cuts at: 1391 2361 3056 3140 3929 1391 Size: 970 695 84 789 4072 Fragments arranged by size: 4072 970 789 695 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3129 928 Size: 971 1230 4409 Fragments arranged by size: 4409 1230 971 BbvCI CC'TCA_GC Cuts at: 2460 2460 Size: 6610 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4801 4835 1903 Size: 34 2864 34 3678 Fragments arranged by size: 3678 2864 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4502 5712 4502 Size: 1210 5400 BclI T'GATC_A Cuts at: 1548 1548 Size: 6610 BfrBI ATG'CAT Cuts at: 3633 3633 Size: 6610 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5221 136 Size: 107 122 71 631 605 3549 1525 Fragments arranged by size: 3549 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3484 6605 3484 Size: 3121 3489 BlpI GC'TnA_GC Cuts at: 4027 4027 Size: 6610 Bme1580I G_kGCm'C Cuts at: 1790 2361 3480 3785 4671 5827 1790 Size: 571 1119 305 886 1156 2573 Fragments arranged by size: 2573 1156 1119 886 571 305 BmgBI CAC'GTC Cuts at: 1318 3901 1318 Size: 2583 4027 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2364 3143 4341 Size: 416 53 83 399 24 947 779 1198 Cuts at: 4341 5299 442 Size: 958 1753 Fragments arranged by size: 1753 1198 958 947 779 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6610 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2453 2486 3637 3691 5270 1239 Size: 1214 33 1151 54 1579 2579 Fragments arranged by size: 2579 1579 1214 1151 54 33 Bpu10I CC'TnA_GC Cuts at: 2460 2460 Size: 6610 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2297 2860 4735 5600 5898 2297 Size: 563 1875 865 298 3009 Fragments arranged by size: 3009 1875 865 563 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5273 882 Size: 617 3774 2219 Fragments arranged by size: 3774 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4401 493 Size: 711 3197 2702 Fragments arranged by size: 3197 2702 711 BsaBI GATnn'nnATC Cuts at: 6604 6604 Size: 6610 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4417 4799 5434 275 Size: 53 83 186 936 2884 382 635 1451 Fragments arranged by size: 2884 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3625 5043 5441 5715 5862 6273 3625 Size: 1418 398 274 147 411 3962 Fragments arranged by size: 3962 1418 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2264 2294 2591 2621 3635 Size: 30 165 30 861 30 297 30 1014 Cuts at: 3635 3665 1178 Size: 30 4123 Fragments arranged by size: 4123 1014 861 297 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2300 3086 3476 1186 Size: 901 213 786 390 4320 Fragments arranged by size: 4320 901 786 390 213 BseYI C'CCAG_C Cuts at: 5813 6309 5813 Size: 496 6114 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2363 2612 2630 1163 Size: 573 627 249 18 5143 Fragments arranged by size: 5143 627 573 249 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4060 4821 5849 1540 Size: 275 186 2059 761 1028 2301 Fragments arranged by size: 2301 2059 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3480 4671 4756 5827 3480 Size: 1191 85 1071 4263 Fragments arranged by size: 4263 1191 1071 85 BsmI GAATG_Cn' Cuts at: 3635 3698 4121 4214 3635 Size: 63 423 93 6031 Fragments arranged by size: 6031 423 93 63 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2361 3056 3140 3480 3785 3929 4671 Size: 399 571 695 84 340 305 144 742 Cuts at: 4671 4756 5827 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 695 571 399 340 305 144 85 84 BspEI T'CCGG_A Cuts at: 3625 6273 3625 Size: 2648 3962 BspHI T'CATG_A Cuts at: 3816 4499 6229 3816 Size: 683 1730 4197 Fragments arranged by size: 4197 1730 683 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2049 2424 3965 844 Size: 1205 375 1541 3489 Fragments arranged by size: 3489 1541 1205 375 BsrBI CCG'CTC Cuts at: 1394 1823 2206 3239 3772 4497 1394 Size: 429 383 1033 533 725 3507 Fragments arranged by size: 3507 1033 725 533 429 383 BsrDI GCAATG_nn' Cuts at: 65 1248 5105 5279 65 Size: 1183 3857 174 1396 Fragments arranged by size: 3857 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2234 2328 2940 5254 5441 1700 Size: 110 424 94 612 2314 187 2869 Fragments arranged by size: 2869 2314 612 424 187 110 94 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6610 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6610 BssSI C'ACGA_G Cuts at: 4670 5682 4670 Size: 1012 5598 BstAPI GCAn_nnn'nTGC Cuts at: 794 3563 794 Size: 2769 3841 BstBI TT'CG_AA Cuts at: 5414 5414 Size: 6610 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6610 BstXI CCAn_nnnn'nTGG Cuts at: 3596 3596 Size: 6610 BstYI r'GATC_y Cuts at: 1972 3484 3940 4307 4692 4709 6161 6247 6259 Size: 1512 456 367 385 17 1452 86 12 Cuts at: 6259 6605 1972 Size: 346 1977 Fragments arranged by size: 1977 1512 1452 456 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2166 2173 2512 3030 3198 3441 Size: 552 939 162 7 339 518 168 243 Cuts at: 3441 3595 513 Size: 154 3528 Fragments arranged by size: 3528 939 552 518 339 243 168 162 154 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2215 3678 487 Size: 683 56 989 1463 3419 Fragments arranged by size: 3419 1463 989 683 56 BtsI GCAGTG_nn' Cuts at: 3557 3873 4126 4920 4940 3557 Size: 316 253 794 20 5227 Fragments arranged by size: 5227 794 316 253 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6610 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6575 DraI TTT'AAA Cuts at: 3914 3914 Size: 6610 DraIII CAC_nnn'GTG Cuts at: 3563 3563 Size: 6610 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5617 809 Size: 507 4301 1802 Fragments arranged by size: 4301 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2908 4057 Size: 54 1425 50 275 18 177 901 1149 Cuts at: 4057 4946 6575 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1149 901 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4057 1537 Size: 275 2245 4090 Fragments arranged by size: 4090 2245 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4540 1353 Size: 3187 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5197 5571 5717 387 Size: 1064 138 3608 374 146 1280 Fragments arranged by size: 3608 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2453 2486 3637 3691 4673 5270 6057 Size: 393 821 33 1151 54 982 597 787 Cuts at: 6057 6325 1239 Size: 268 1524 Fragments arranged by size: 1524 1151 982 821 787 597 393 268 54 33 EcoNI CCTnn'n_nnAGG Cuts at: 2093 2931 2093 Size: 838 5772 EcoO109I rG'GnC_Cy Cuts at: 2244 2358 3008 2244 Size: 114 650 5846 Fragments arranged by size: 5846 650 114 EcoRI G'AATT_C Cuts at: 3919 4297 3919 Size: 378 6232 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2338 2338 Size: 6610 FspI TGC'GCA Cuts at: 3884 5116 3884 Size: 1232 5378 HaeII r_GCGC'y Cuts at: 1678 2034 5757 1678 Size: 356 3723 2531 Fragments arranged by size: 3723 2531 356 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3314 3346 3796 3828 5251 Size: 32 135 32 1742 32 450 32 1423 Cuts at: 5251 5283 5325 5357 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1742 1423 450 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3954 4200 669 Size: 988 281 2016 246 3079 Fragments arranged by size: 3079 2016 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6610 HpaI GTT'AAC Cuts at: 4200 4200 Size: 6610 KpnI G_GTAC'C Cuts at: 3935 3935 Size: 6610 MfeI C'AATT_G Cuts at: 4209 4209 Size: 6610 MluI A'CGCG_T Cuts at: 6486 6486 Size: 6610 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5724 5908 1305 Size: 4419 184 2007 Fragments arranged by size: 4419 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2910 10 Size: 54 1425 343 177 901 3710 Fragments arranged by size: 3710 1425 901 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3041 3446 3752 4568 4927 5086 518 Size: 648 1875 405 306 816 359 159 2042 Fragments arranged by size: 2042 1875 816 648 405 359 306 159 MspA1I CmG'CkG Cuts at: 1606 2016 2464 2861 3283 4026 4703 5851 6096 Size: 410 448 397 422 743 677 1148 245 Cuts at: 6096 1606 Size: 2120 Fragments arranged by size: 2120 1148 743 677 448 422 410 397 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2512 3030 3441 3595 513 Size: 552 939 508 518 411 154 3528 Fragments arranged by size: 3528 939 552 518 508 411 154 NdeI CA'TA_TG Cuts at: 387 387 Size: 6610 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6610 NotI GC'GGCC_GC Cuts at: 4057 4057 Size: 6610 NsiI A_TGCA'T Cuts at: 3635 3635 Size: 6610 NspI r_CATG'y Cuts at: 1132 1229 3401 3968 5513 1132 Size: 97 2172 567 1545 2229 Fragments arranged by size: 2229 2172 1545 567 97 PasI CC'CwG_GG Cuts at: 1348 2991 1348 Size: 1643 4967 PciI A'CATG_T Cuts at: 3397 5509 3397 Size: 2112 4498 PflMI CCAn_nnn'nTGG Cuts at: 1108 2906 1108 Size: 1798 4812 PfoI T'CCnGG_A Cuts at: 3332 3332 Size: 6610 PmeI GTTT'AAAC Cuts at: 3914 3914 Size: 6610 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4679 4711 6192 6224 4679 Size: 32 1481 32 5065 Fragments arranged by size: 5065 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2244 2244 Size: 6610 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6610 PsiI TTA'TAA Cuts at: 2742 4180 2742 Size: 1438 5172 PspOMI G'GGCC_C Cuts at: 2357 2357 Size: 6610 PstI C_TGCA'G Cuts at: 830 2421 3001 3790 3962 830 Size: 1591 580 789 172 3478 Fragments arranged by size: 3478 1591 789 580 172 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6610 PvuII CAG'CTG Cuts at: 2464 3283 2464 Size: 819 5791 SalI G'TCGA_C Cuts at: 1936 3952 1936 Size: 2016 4594 SbfI CC_TGCA'GG Cuts at: 2421 3790 3962 2421 Size: 1369 172 5069 Fragments arranged by size: 5069 1369 172 ScaI AGT'ACT Cuts at: 2953 3455 4858 6570 2953 Size: 502 1403 1712 2993 Fragments arranged by size: 2993 1712 1403 502 SexAI A'CCwGG_T Cuts at: 2088 2088 Size: 6610 SfcI C'TryA_G Cuts at: 826 1045 2417 2997 3786 3958 5093 5774 5965 Size: 219 1372 580 789 172 1135 681 191 Cuts at: 5965 826 Size: 1471 Fragments arranged by size: 1471 1372 1135 789 681 580 219 191 172 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6610 SgrAI Cr'CCGG_yG Cuts at: 1700 2234 1700 Size: 534 6076 SmaI CCC'GGG Cuts at: 1872 2069 3937 1872 Size: 197 1868 4545 Fragments arranged by size: 4545 1868 197 SmlI C'TyrA_G Cuts at: 820 1017 1943 2312 2875 4714 5615 5877 820 Size: 197 926 369 563 1839 901 262 1553 Fragments arranged by size: 1839 1553 926 901 563 369 262 197 SnaBI TAC'GTA Cuts at: 493 4401 493 Size: 3908 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6610 SphI G_CATG'C Cuts at: 1132 3968 1132 Size: 2836 3774 SspI AAT'ATT Cuts at: 5 52 4534 5 Size: 47 4482 2081 Fragments arranged by size: 4482 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6610 StyI C'CwwG_G Cuts at: 513 1065 2004 2335 2504 2512 2932 3030 3084 Size: 552 939 331 169 8 420 98 54 Cuts at: 3084 3441 3595 4049 513 Size: 357 154 454 3074 Fragments arranged by size: 3074 939 552 454 420 357 331 169 154 98 54 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2138 3117 4637 4654 4807 4992 5437 Size: 1255 558 979 1520 17 153 185 445 Cuts at: 5437 325 Size: 1498 Fragments arranged by size: 1520 1498 1255 979 558 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2951 3453 4856 Size: 274 80 33 51 1443 974 502 1403 Cuts at: 4856 6568 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1403 974 502 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2264 2296 2903 2935 6570 6602 2264 Size: 32 607 32 3635 32 2272 Fragments arranged by size: 3635 2272 607 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2854 1316 Size: 1538 5072 XbaI T'CTAG_A Cuts at: 3946 3946 Size: 6610 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2017 2291 2017 Size: 274 6336 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6610 XmaI C'CCGG_G Cuts at: 1870 2067 3935 1870 Size: 197 1868 4545 Fragments arranged by size: 4545 1868 197 XmnI GAAnn'nnTTC Cuts at: 2321 3426 4739 2321 Size: 1105 1313 4192 Fragments arranged by size: 4192 1313 1105 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4418 276 Size: 53 83 186 936 2884 2468 Fragments arranged by size: 2884 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI