(Circular) (Six-base) MAPSORT of: pFN21AB3349.seq Check: 3051 from: 1 to: 9286 pFN21AB3349 9286 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3532 4792 5698 6222 3532 Size: 1260 906 524 6596 Fragments arranged by size: 6596 1260 906 524 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7096 278 Size: 53 83 186 936 5560 2468 Fragments arranged by size: 5560 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2610 5014 6629 1937 Size: 673 2404 1615 4594 Fragments arranged by size: 4594 2404 1615 673 Acc65I G'GTAC_C Cuts at: 6607 6607 Size: 9286 AclI AA'CG_TT Cuts at: 1563 7413 7786 1563 Size: 5850 373 3063 Fragments arranged by size: 5850 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2557 2805 2924 3527 3557 4103 5432 7349 Size: 925 248 119 603 30 546 1329 1917 Cuts at: 7349 8733 9001 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1917 1384 1329 925 603 546 268 248 119 30 AfeI AGC'GCT Cuts at: 2638 3260 4160 5384 2638 Size: 622 900 1224 6540 Fragments arranged by size: 6540 1224 900 622 AflII C'TTAA_G Cuts at: 820 1017 3736 820 Size: 197 2719 6370 Fragments arranged by size: 6370 2719 197 AflIII A'CryG_T Cuts at: 1203 3666 6219 8185 9162 1203 Size: 2463 2553 1966 977 1327 Fragments arranged by size: 2553 2463 1966 1327 977 AgeI A'CCGG_T Cuts at: 5580 8117 5580 Size: 2537 6749 AhdI GACnn_n'nnGTC Cuts at: 2725 4336 8015 2725 Size: 1611 3679 3996 Fragments arranged by size: 3996 3679 1611 AleI CACnn'nnGTG Cuts at: 1166 4694 5306 5375 6224 1166 Size: 3528 612 69 849 4228 Fragments arranged by size: 4228 3528 849 612 69 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3518 1184 Size: 2334 6952 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5012 5044 5204 5236 5858 5890 5012 Size: 32 160 32 622 32 8408 Fragments arranged by size: 8408 622 160 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2770 2773 2831 2949 2971 3097 3577 3922 4174 Size: 3 58 118 22 126 480 345 252 Cuts at: 4174 4986 5146 5471 7029 8601 2770 Size: 812 160 325 1558 1572 3455 Fragments arranged by size: 3455 1572 1558 812 480 345 325 252 160 126 118 58 22 3 ApaI G_GGCC'C Cuts at: 2305 3916 4505 2305 Size: 1611 589 7086 Fragments arranged by size: 7086 1611 589 ApaLI G'TGCA_C Cuts at: 2066 4058 5942 7343 8499 2066 Size: 1992 1884 1401 1156 2853 Fragments arranged by size: 2853 1992 1884 1401 1156 ApoI r'AATT_y Cuts at: 1493 5061 6512 6595 6821 6973 9082 1493 Size: 3568 1451 83 226 152 2109 1697 Fragments arranged by size: 3568 2109 1697 1451 226 152 83 AseI AT'TA_AT Cuts at: 160 1032 7840 160 Size: 872 6808 1606 Fragments arranged by size: 6808 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9286 AvaI C'yCGr_G Cuts at: 1870 1943 4290 5671 6133 6315 6611 1870 Size: 73 2347 1381 462 182 296 4545 Fragments arranged by size: 4545 2347 1381 462 296 182 73 AvrII C'CTAG_G Cuts at: 3346 3346 Size: 9286 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5520 5553 5520 Size: 33 9253 BamHI G'GATC_C Cuts at: 3807 4101 6616 6983 3807 Size: 294 2515 367 6110 Fragments arranged by size: 6110 2515 367 294 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 2175 2870 3899 4581 Size: 325 217 627 335 53 695 1029 682 Cuts at: 4581 4776 5474 5812 6607 8062 618 Size: 195 698 338 795 1455 1842 Fragments arranged by size: 1842 1455 1029 795 698 695 682 627 338 335 325 217 195 53 BanII G_rGCy'C Cuts at: 1391 2034 2305 3528 3570 3916 4073 4505 4872 Size: 643 271 1223 42 346 157 432 367 Cuts at: 4872 4908 6233 6322 6605 1391 Size: 36 1325 89 283 4072 Fragments arranged by size: 4072 1325 1223 643 432 367 346 283 271 157 89 42 36 BbeI G_GCGC'C Cuts at: 5816 5816 Size: 9286 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2394 4650 5985 928 Size: 971 495 2256 1335 4229 Fragments arranged by size: 4229 2256 1335 971 495 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2165 2199 7477 7511 1903 Size: 34 228 34 5278 34 3678 Fragments arranged by size: 5278 3678 228 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7178 8388 7178 Size: 1210 8076 BclI T'GATC_A Cuts at: 1548 3009 3648 1548 Size: 1461 639 7186 Fragments arranged by size: 7186 1461 639 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2915 3704 7897 Size: 107 122 71 631 605 1243 789 4193 Cuts at: 7897 136 Size: 1525 Fragments arranged by size: 4193 1525 1243 789 631 605 122 107 71 BglII A'GATC_T Cuts at: 2496 9281 2496 Size: 6785 2501 BlpI GC'TnA_GC Cuts at: 2999 5588 6703 2999 Size: 2589 1115 5582 Fragments arranged by size: 5582 2589 1115 Bme1580I G_kGCm'C Cuts at: 1790 2070 2127 2305 2875 3916 3970 4062 4089 Size: 280 57 178 570 1041 54 92 27 Cuts at: 4089 4505 4584 4732 4831 5946 7347 8503 1790 Size: 416 79 148 99 1115 1401 1156 2573 Fragments arranged by size: 2573 1401 1156 1115 1041 570 416 280 178 148 99 92 79 57 54 27 BmgBI CAC'GTC Cuts at: 1318 4381 1318 Size: 3063 6223 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3144 3162 3757 Size: 416 53 83 399 24 1727 18 595 Cuts at: 3757 5988 6409 7017 7975 442 Size: 2231 421 608 958 1753 Fragments arranged by size: 2231 1753 1727 958 608 595 421 416 399 83 53 24 18 BmtI G_CTAG'C Cuts at: 1056 4196 4478 1056 Size: 3140 282 5864 Fragments arranged by size: 5864 3140 282 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2053 6341 2053 Size: 4288 4998 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2729 2795 2914 3387 3554 4196 4223 4607 Size: 1490 66 119 473 167 642 27 384 Cuts at: 4607 6044 6398 6507 7946 1239 Size: 1437 354 109 1439 2579 Fragments arranged by size: 2579 1490 1439 1437 642 473 384 354 167 119 109 66 27 Bpu10I CC'TnA_GC Cuts at: 3821 3821 Size: 9286 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2204 7411 8276 8574 2204 Size: 5207 865 298 2916 Fragments arranged by size: 5207 2916 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2624 5919 7949 882 Size: 617 1125 3295 2030 2219 Fragments arranged by size: 3295 2219 2030 1125 617 BsaAI yAC'GTr Cuts at: 493 1204 3667 3673 5861 7077 493 Size: 711 2463 6 2188 1216 2702 Fragments arranged by size: 2702 2463 2188 1216 711 6 BsaBI GATnn'nnATC Cuts at: 2684 9280 2684 Size: 6596 2690 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 3696 5043 5813 Size: 53 83 186 936 477 1686 1347 770 Cuts at: 5813 7093 7475 8110 275 Size: 1280 382 635 1451 Fragments arranged by size: 1686 1451 1347 1280 936 770 635 477 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5580 5877 7719 8117 8391 8538 8949 5580 Size: 297 1842 398 274 147 411 5917 Fragments arranged by size: 5917 1842 411 398 297 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2960 2990 4956 4986 5204 Size: 30 165 30 1557 30 1966 30 218 Cuts at: 5204 5234 5282 5312 5558 5588 5858 5888 1178 Size: 30 48 30 246 30 270 30 4576 Fragments arranged by size: 4576 1966 1557 270 246 218 165 48 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2726 4856 5671 6194 1186 Size: 1540 2130 815 523 4278 Fragments arranged by size: 4278 2130 1540 815 523 BseYI C'CCAG_C Cuts at: 2866 3062 3255 3527 4027 4089 4510 5038 6234 Size: 196 193 272 500 62 421 528 1196 Cuts at: 6234 8489 8985 2866 Size: 2255 496 3167 Fragments arranged by size: 3167 2255 1196 528 500 496 421 272 196 193 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2783 3646 3920 4096 4635 4820 5399 Size: 573 1047 863 274 176 539 185 579 Cuts at: 5399 5619 6200 6412 1163 Size: 220 581 212 4037 Fragments arranged by size: 4037 1047 863 581 579 573 539 274 220 212 185 176 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5903 6736 7497 8525 1540 Size: 275 186 3902 833 761 1028 2301 Fragments arranged by size: 3902 2301 1028 833 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 2070 3083 3570 4062 4872 5367 5628 5946 Size: 36 1013 487 492 810 495 261 318 Cuts at: 5946 6233 6263 7347 7432 8503 2034 Size: 287 30 1084 85 1071 2817 Fragments arranged by size: 2817 1084 1071 1013 810 495 492 487 318 287 261 85 36 30 BsmI GAATG_Cn' Cuts at: 6797 6890 6797 Size: 93 9193 BsmBI CGTCTCn'nnnn_ Cuts at: 2257 2257 Size: 9286 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2070 2127 2305 2875 3083 3528 Size: 399 244 36 57 178 570 208 445 Cuts at: 3528 3570 3916 3970 4062 4073 4089 4505 4584 Size: 42 346 54 92 11 16 416 79 Cuts at: 4584 4732 4831 4872 4908 5367 5628 5946 6233 Size: 148 99 41 36 459 261 318 287 Cuts at: 6233 6263 6322 6605 7347 7432 8503 1391 Size: 30 59 283 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 570 459 445 416 399 346 318 287 283 261 244 208 178 148 99 92 85 79 59 57 54 42 41 36 36 30 16 11 BspEI T'CCGG_A Cuts at: 5877 8949 5877 Size: 3072 6214 BspHI T'CATG_A Cuts at: 7175 8905 7175 Size: 1730 7556 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2178 2979 3532 3930 4792 5698 6096 6222 Size: 1334 801 553 398 862 906 398 126 Cuts at: 6222 6434 6641 844 Size: 212 207 3489 Fragments arranged by size: 3489 1334 906 862 801 553 398 398 212 207 126 BsrBI CCG'CTC Cuts at: 1394 1823 2383 2463 5332 5436 7173 1394 Size: 429 560 80 2869 104 1737 3507 Fragments arranged by size: 3507 2869 1737 560 429 104 80 BsrDI GCAATG_nn' Cuts at: 65 1248 6534 7781 7955 65 Size: 1183 5286 1247 174 1396 Fragments arranged by size: 5286 1396 1247 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2697 5580 5898 6238 6450 7930 8117 Size: 110 887 2883 318 340 212 1480 187 Cuts at: 8117 1700 Size: 2869 Fragments arranged by size: 2883 2869 1480 887 340 318 212 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9286 BssHII G'CGCG_C Cuts at: 1925 3424 1925 Size: 1499 7787 BssSI C'ACGA_G Cuts at: 6302 7346 8358 6302 Size: 1044 1012 7230 Fragments arranged by size: 7230 1044 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2770 5642 794 Size: 1976 2872 4438 Fragments arranged by size: 4438 2872 1976 BstBI TT'CG_AA Cuts at: 8090 8090 Size: 9286 BstEII G'GTnAC_C Cuts at: 1184 2344 3640 1184 Size: 1160 1296 6830 Fragments arranged by size: 6830 1296 1160 BstXI CCAn_nnnn'nTGG Cuts at: 2186 2356 3031 4900 5101 5308 6376 6484 2186 Size: 170 675 1869 201 207 1068 108 4988 Fragments arranged by size: 4988 1869 1068 675 207 201 170 108 BstYI r'GATC_y Cuts at: 1972 2469 2496 2747 3483 3807 4101 4666 6616 Size: 497 27 251 736 324 294 565 1950 Cuts at: 6616 6983 7368 7385 8837 8923 8935 9281 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1950 1452 736 565 497 385 367 346 324 294 251 86 27 17 12 Bsu36I CC'TnA_GG Cuts at: 5033 5033 Size: 9286 BtgI C'CryG_G Cuts at: 513 1065 2004 2281 2293 3030 3978 4074 4341 Size: 552 939 277 12 737 948 96 267 Cuts at: 4341 4899 5001 5094 5100 513 Size: 558 102 93 6 4699 Fragments arranged by size: 4699 948 939 737 558 552 277 267 102 96 93 12 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 6427 487 Size: 683 56 5201 3346 Fragments arranged by size: 5201 3346 683 56 BtsI GCAGTG_nn' Cuts at: 4631 5144 6802 7596 7616 4631 Size: 513 1658 794 20 6301 Fragments arranged by size: 6301 1658 794 513 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9286 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9251 DraI TTT'AAA Cuts at: 6590 6590 Size: 9286 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3953 5234 8293 809 Size: 507 2637 1281 3059 1802 Fragments arranged by size: 3059 2637 1802 1281 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2296 2358 2682 Size: 54 1425 50 275 18 466 62 324 Cuts at: 2682 2916 3411 3975 4359 4920 5097 5319 5900 Size: 234 495 564 384 561 177 222 581 Cuts at: 5900 6564 6733 7622 9251 8 Size: 664 169 889 1629 43 Fragments arranged by size: 1629 1425 889 664 581 564 561 495 466 384 324 275 234 222 177 169 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5900 6733 1537 Size: 275 4088 833 4090 Fragments arranged by size: 4090 4088 833 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2733 4122 5339 7216 1353 Size: 1380 1389 1217 1877 3423 Fragments arranged by size: 3423 1877 1389 1380 1217 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2276 7873 8247 8393 387 Size: 1064 138 687 5597 374 146 1280 Fragments arranged by size: 5597 1280 1064 687 374 146 138 EcoICRI GAG'CTC Cuts at: 2032 3568 4870 6231 2032 Size: 1536 1302 1361 5087 Fragments arranged by size: 5087 1536 1361 1302 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2557 2729 2795 2805 2914 2924 3387 Size: 393 925 172 66 10 109 10 463 Cuts at: 3387 3527 3554 3557 4103 4196 4223 4607 5432 Size: 140 27 3 546 93 27 384 825 Cuts at: 5432 6044 6398 6507 7349 7946 8733 9001 1239 Size: 612 354 109 842 597 787 268 1524 Fragments arranged by size: 1524 925 842 825 787 612 597 546 463 393 384 354 268 172 140 109 109 93 66 27 27 10 10 3 EcoNI CCTnn'n_nnAGG Cuts at: 2572 3539 3782 5844 6395 2572 Size: 967 243 2062 551 5463 Fragments arranged by size: 5463 2062 967 551 243 EcoO109I rG'GnC_Cy Cuts at: 2302 2410 2719 2883 2908 2968 3350 3919 3955 Size: 108 309 164 25 60 382 569 36 Cuts at: 3955 3986 6118 6138 6481 2302 Size: 31 2132 20 343 5107 Fragments arranged by size: 5107 2132 569 382 343 309 164 108 60 36 31 25 20 EcoRI G'AATT_C Cuts at: 5061 6595 6973 5061 Size: 1534 378 7374 Fragments arranged by size: 7374 1534 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2914 3851 4387 2914 Size: 937 536 7813 Fragments arranged by size: 7813 937 536 FspI TGC'GCA Cuts at: 3581 6146 7792 3581 Size: 2565 1646 5075 Fragments arranged by size: 5075 2565 1646 HaeII r_GCGC'y Cuts at: 1678 2640 3262 4162 5386 5816 8433 1678 Size: 962 622 900 1224 430 2617 2531 Fragments arranged by size: 2617 2531 1224 962 900 622 430 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2711 2732 2743 2764 2960 Size: 32 135 32 1139 21 11 21 196 Cuts at: 2960 2992 3732 3764 4322 4354 5704 5736 5741 Size: 32 740 32 558 32 1350 32 5 Cuts at: 5741 5773 6077 6109 7927 7959 8001 8033 1373 Size: 32 304 32 1818 32 42 32 2626 Fragments arranged by size: 2626 1818 1350 1139 740 558 304 196 135 42 32 32 32 32 32 32 32 32 32 32 21 21 11 5 HincII GTy'rAC Cuts at: 669 1657 1938 2611 3235 6630 6876 669 Size: 988 281 673 624 3395 246 3079 Fragments arranged by size: 3395 3079 988 673 624 281 246 HindIII A'AGCT_T Cuts at: 748 2957 748 Size: 2209 7077 HpaI GTT'AAC Cuts at: 6876 6876 Size: 9286 KasI G'GCGC_C Cuts at: 5812 5812 Size: 9286 KpnI G_GTAC'C Cuts at: 6611 6611 Size: 9286 MfeI C'AATT_G Cuts at: 6885 6885 Size: 9286 MluI A'CGCG_T Cuts at: 9162 9162 Size: 9286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2079 3048 4587 5601 8400 8584 1305 Size: 774 969 1539 1014 2799 184 2007 Fragments arranged by size: 2799 2007 1539 1014 969 774 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2360 2684 2918 4361 4922 Size: 54 1425 343 528 324 234 1443 561 Cuts at: 4922 5099 5321 6566 10 Size: 177 222 1245 2730 Fragments arranged by size: 2730 1443 1425 1245 561 528 343 324 234 222 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2184 2678 3128 3245 3500 3620 4469 Size: 648 1018 494 450 117 255 120 849 Cuts at: 4469 4694 5099 5267 5306 5375 5597 6224 6464 Size: 225 405 168 39 69 222 627 240 Cuts at: 6464 7244 7603 7762 518 Size: 780 359 159 2042 Fragments arranged by size: 2042 1018 849 780 648 627 494 450 405 359 255 240 225 222 168 159 120 117 69 39 MspA1I CmG'CkG Cuts at: 1606 2770 2995 3574 4270 4276 4343 4596 4723 Size: 1164 225 579 696 6 67 253 127 Cuts at: 4723 5108 6100 6365 6702 7379 8527 8772 1606 Size: 385 992 265 337 677 1148 245 2120 Fragments arranged by size: 2120 1164 1148 992 696 677 579 385 337 265 253 245 225 127 67 6 NaeI GCC'GGC Cuts at: 6240 6240 Size: 9286 NarI GG'CG_CC Cuts at: 5813 5813 Size: 9286 NcoI C'CATG_G Cuts at: 513 1065 2004 2281 2293 3030 5001 5094 5100 Size: 552 939 277 12 737 1971 93 6 Cuts at: 5100 513 Size: 4699 Fragments arranged by size: 4699 1971 939 737 552 277 93 12 6 NdeI CA'TA_TG Cuts at: 387 387 Size: 9286 NgoMIV G'CCGG_C Cuts at: 6238 6238 Size: 9286 NheI G'CTAG_C Cuts at: 1052 4192 4474 1052 Size: 3140 282 5864 Fragments arranged by size: 5864 3140 282 NotI GC'GGCC_GC Cuts at: 6733 6733 Size: 9286 NspI r_CATG'y Cuts at: 1132 1229 3757 5942 6644 8189 1132 Size: 97 2528 2185 702 1545 2229 Fragments arranged by size: 2528 2229 2185 1545 702 97 PasI CC'CwG_GG Cuts at: 1348 5817 1348 Size: 4469 4817 PciI A'CATG_T Cuts at: 8185 8185 Size: 9286 PflMI CCAn_nnn'nTGG Cuts at: 1108 2046 2287 2758 3850 4974 5583 1108 Size: 938 241 471 1092 1124 609 4811 Fragments arranged by size: 4811 1124 1092 938 609 471 241 PfoI T'CCnGG_A Cuts at: 2964 6021 6489 2964 Size: 3057 468 5761 Fragments arranged by size: 5761 3057 468 PmeI GTTT'AAAC Cuts at: 6590 6590 Size: 9286 PmlI CAC'GTG Cuts at: 5861 5861 Size: 9286 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5204 5236 5858 5890 7355 7387 8868 8900 5204 Size: 32 622 32 1465 32 1481 32 5590 Fragments arranged by size: 5590 1481 1465 622 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2410 2719 2968 3350 3919 3955 6118 6138 6481 Size: 309 249 382 569 36 2163 20 343 Cuts at: 6481 2410 Size: 5215 Fragments arranged by size: 5215 2163 569 382 343 309 249 36 20 PshAI GACnn'nnGTC Cuts at: 1613 4489 5931 1613 Size: 2876 1442 4968 Fragments arranged by size: 4968 2876 1442 PsiI TTA'TAA Cuts at: 6856 6856 Size: 9286 PspOMI G'GGCC_C Cuts at: 2301 3912 4501 2301 Size: 1611 589 7086 Fragments arranged by size: 7086 1611 589 PspXI vC'TCGA_Gb Cuts at: 4290 4290 Size: 9286 PstI C_TGCA'G Cuts at: 830 2391 3312 4524 6638 830 Size: 1561 921 1212 2114 3478 Fragments arranged by size: 3478 2114 1561 1212 921 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9286 PvuII CAG'CTG Cuts at: 2770 2995 3574 4596 4723 6100 6365 2770 Size: 225 579 1022 127 1377 265 5691 Fragments arranged by size: 5691 1377 1022 579 265 225 127 SacI G_AGCT'C Cuts at: 2034 3570 4872 6233 2034 Size: 1536 1302 1361 5087 Fragments arranged by size: 5087 1536 1361 1302 SacII CC_GC'GG Cuts at: 4344 4344 Size: 9286 SalI G'TCGA_C Cuts at: 1936 2609 6628 1936 Size: 673 4019 4594 Fragments arranged by size: 4594 4019 673 SanDI GG'GwC_CC Cuts at: 6138 6138 Size: 9286 SapI GCTCTTCn'nnn_ Cuts at: 5339 5339 Size: 9286 SbfI CC_TGCA'GG Cuts at: 6638 6638 Size: 9286 ScaI AGT'ACT Cuts at: 5792 7534 9246 5792 Size: 1742 1712 5832 Fragments arranged by size: 5832 1742 1712 SexAI A'CCwGG_T Cuts at: 5913 5913 Size: 9286 SfcI C'TryA_G Cuts at: 826 1045 2387 3172 3308 4520 6634 7769 8450 Size: 219 1342 785 136 1212 2114 1135 681 Cuts at: 8450 8641 826 Size: 191 1471 Fragments arranged by size: 2114 1471 1342 1212 1135 785 681 219 191 136 SfiI GGCCn_nnn'nGGCC Cuts at: 2915 2915 Size: 9286 SfoI GGC'GCC Cuts at: 5814 5814 Size: 9286 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9286 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9286 SmaI CCC'GGG Cuts at: 1872 5673 6613 1872 Size: 3801 940 4545 Fragments arranged by size: 4545 3801 940 SmlI C'TyrA_G Cuts at: 820 1017 1943 2219 3736 4290 7390 8291 8553 Size: 197 926 276 1517 554 3100 901 262 Cuts at: 8553 820 Size: 1553 Fragments arranged by size: 3100 1553 1517 926 901 554 276 262 197 SnaBI TAC'GTA Cuts at: 493 7077 493 Size: 6584 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9286 SphI G_CATG'C Cuts at: 1132 6644 1132 Size: 5512 3774 SspI AAT'ATT Cuts at: 5 52 7210 5 Size: 47 7158 2081 Fragments arranged by size: 7158 2081 47 StuI AGG'CCT Cuts at: 1517 3707 4181 6246 1517 Size: 2190 474 2065 4557 Fragments arranged by size: 4557 2190 2065 474 StyI C'CwwG_G Cuts at: 513 1065 2004 2281 2293 2331 2481 3030 3346 Size: 552 939 277 12 38 150 549 316 Cuts at: 3346 4576 4893 5001 5094 5100 5223 6725 513 Size: 1230 317 108 93 6 123 1502 3074 Fragments arranged by size: 3074 1502 1230 939 552 549 317 316 277 150 123 108 93 38 12 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2110 2222 2858 3152 3649 5809 6428 Size: 1255 530 112 636 294 497 2160 619 Cuts at: 6428 7313 7330 7483 7668 8113 325 Size: 885 17 153 185 445 1498 Fragments arranged by size: 2160 1498 1255 885 636 619 530 497 445 294 185 153 112 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5790 7532 9244 Size: 274 80 33 51 1443 3813 1742 1712 Cuts at: 9244 96 Size: 138 Fragments arranged by size: 3813 1742 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 3148 3180 4956 4988 6176 6208 6242 Size: 32 1116 32 1776 32 1188 32 34 Cuts at: 6242 6274 9246 9278 2000 Size: 32 2972 32 2008 Fragments arranged by size: 2972 2008 1776 1188 1116 34 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4379 4755 1316 Size: 3063 376 5847 Fragments arranged by size: 5847 3063 376 XbaI T'CTAG_A Cuts at: 6622 6622 Size: 9286 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2221 4861 2221 Size: 2640 6646 XhoI C'TCGA_G Cuts at: 1943 4290 1943 Size: 2347 6939 XmaI C'CCGG_G Cuts at: 1870 5671 6611 1870 Size: 3801 940 4545 Fragments arranged by size: 4545 3801 940 XmnI GAAnn'nnTTC Cuts at: 5216 7415 5216 Size: 2199 7087 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7094 276 Size: 53 83 186 936 5560 2468 Fragments arranged by size: 5560 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BbvCI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PsrI RsrII SrfI SwaI