Restriction map for
pFN21AB3334
##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AvrII BbeI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BsaI BseAI BsgI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FbaI FseI HindIII HpaI
KasI KspI MaeI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1723 GACGTC GACGT^C
Acc65I 57 GGTACC G^GTACC
AccI 226 GTCTAC GT^[AC][GT]AC
AccII 1495 CGCG CG^CG
AflIII 1466 ACGTGT A^C[GA][CT]GT
Asp718I 57 GGTACC G^GTACC
AviII 1544 TGCGCA TGC^GCA
BamHI 1554 GGATCC G^GATCC
BanI 57 GGTACC G^G[CT][GA]CC
BanII 1813 GAGCTC G[GA]GC[CT]^C
BbrPI 1465 CACGTG CAC^GTG
BbsI 664 GAAGAC GAAGAC(2/6)
BcgI 862 CGAGAGGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 7 CCCGG CC^[GC]GG
BglI 34 GCCTGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1994 AGATCT A^GATCT
BpuAI 664 GAAGAC GAAGAC(2/6)
BsaAI 1465 CACGTG [CT]AC^GT[GA]
BsiEI 1938 CGACCG CG[GA][CT]^CG
BsmBI 886 GAGACG CGTCTC(1/5)
BsmI 1863 GCATTC GAATGC(1/-1)
BspHI 1226 TCATGA T^CATGA
BspMI 181 ACCTGC ACCTGC(4/8)
BstAPI 305 GCAAGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1495 CGCG CG^CG
DraIII 328 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 2020 GAATTC G^AATTC
FspI 1544 TGCGCA TGC^GCA
HgaI 2215 GACGC GACGC(5/10)
KpnI 57 GGTACC GGTAC^C
MseI 1085 TTAA T^TAA
MvnI 1495 CGCG CG^CG
NciI 7 CCCGG CC^[GC]GG
PaeR7I 1135 CTCGAG C^TCGAG
PflMI 1960 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1465 CACGTG CAC^GTG
PmlI 1465 CACGTG CAC^GTG
PvuII 1354 CAGCTG CAG^CTG
RcaI 1226 TCATGA T^CATGA
SacI 1813 GAGCTC GAGCT^C
ScaI 1004 AGTACT AGT^ACT
SexAI 1959 ACCAGGT A^CC[AT]GGT
Tru9I 1085 TTAA T^TAA
Van91I 1960 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 836 CCACAGAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1135 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 400 AAATTC [GA]^AATT[CT]
2020 GAATTC
AcyI 1723 GACGTC G[GA]^CG[CT]C
2215 GACGCC
ApoI 400 AAATTC [GA]^AATT[CT]
2020 GAATTC
AspHI 870 GTGCTC G[AT]GC[AT]^C
1813 GAGCTC
AvaII 173 GGACC G^G[AT]CC
1580 GGTCC
BalI 1373 TGGCCA TGG^CCA
1730 TGGCCA
BbvCI 703 GCTGAGG CCTCAGC(-5/-2)
772 GCTGAGG
BsaBI 157 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2145 GATCGTCATC
BsaHI 1723 GACGTC G[GA]^CG[CT]C
2215 GACGCC
BsaWI 980 ACCGGA [AT]^CCGG[AT]
1577 TCCGGT
BsiHKAI 870 GTGCTC G[AT]GC[AT]^C
1813 GAGCTC
BsrBI 1433 CCGCTC CCGCTC(-3/-3)
1568 CCGCTC
BsrDI 813 CATTGC GCAATG(2/0)
904 GCAATG
DrdI 613 GACTCGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2215 GACGCCGCTGTC
DsaI 1808 CCGTGG C^C[GA][CT]GG
1858 CCGTGG
Hin1I 1723 GACGTC G[GA]^CG[CT]C
2215 GACGCC
MamI 157 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2145 GATCGTCATC
MluNI 1373 TGGCCA TGG^CCA
1730 TGGCCA
MscI 1373 TGGCCA TGG^CCA
1730 TGGCCA
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI BbvI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII