(Circular) (Six-base) MAPSORT of: pFN21AB3334.seq Check: 4646 from: 1 to: 7015 pFN21AB3334 7015 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3732 4825 278 Size: 53 83 186 936 2196 1093 2468 Fragments arranged by size: 2468 2196 1093 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2232 4358 1937 Size: 295 2126 4594 Fragments arranged by size: 4594 2126 295 Acc65I G'GTAC_C Cuts at: 2062 4336 2062 Size: 2274 4741 AclI AA'CG_TT Cuts at: 1563 4314 5142 5515 1563 Size: 2751 828 373 3063 Fragments arranged by size: 3063 2751 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2595 2961 3686 4026 4193 5078 6462 6730 Size: 963 366 725 340 167 885 1384 268 Cuts at: 6730 1632 Size: 1917 Fragments arranged by size: 1917 1384 963 885 725 366 340 268 167 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6818 AflIII A'CryG_T Cuts at: 1203 3471 5914 6891 1203 Size: 2268 2443 977 1327 Fragments arranged by size: 2443 2268 1327 977 AgeI A'CCGG_T Cuts at: 5846 5846 Size: 7015 AhdI GACnn_n'nnGTC Cuts at: 5744 5744 Size: 7015 AleI CACnn'nnGTG Cuts at: 1166 2255 3606 4163 1166 Size: 1089 1351 557 4018 Fragments arranged by size: 4018 1351 1089 557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2534 1184 Size: 1350 5665 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4035 4067 4035 Size: 32 6983 AlwNI CAG_nnn'CTG Cuts at: 2849 3361 3971 4758 6330 2849 Size: 512 610 787 1572 3534 Fragments arranged by size: 3534 1572 787 610 512 ApaLI G'TGCA_C Cuts at: 5072 6228 5072 Size: 1156 5859 ApoI r'AATT_y Cuts at: 1493 2405 4025 4324 4550 4702 6811 1493 Size: 912 1620 299 226 152 2109 1697 Fragments arranged by size: 2109 1697 1620 912 299 226 152 AseI AT'TA_AT Cuts at: 160 1032 5569 160 Size: 872 4537 1606 Fragments arranged by size: 4537 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7015 AvaI C'yCGr_G Cuts at: 1870 1943 2049 3078 3140 4340 1870 Size: 73 106 1029 62 1200 4545 Fragments arranged by size: 4545 1200 1029 106 73 62 BamHI G'GATC_C Cuts at: 3559 4345 4712 3559 Size: 786 367 5862 Fragments arranged by size: 5862 786 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2062 4336 5791 618 Size: 325 217 627 275 2274 1455 1842 Fragments arranged by size: 2274 1842 1455 627 325 275 217 BanII G_rGCy'C Cuts at: 1391 3822 4334 1391 Size: 2431 512 4072 Fragments arranged by size: 4072 2431 512 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2676 928 Size: 971 777 5267 Fragments arranged by size: 5267 971 777 BbvCI CC'TCA_GC Cuts at: 2709 2778 2709 Size: 69 6946 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2856 2890 5206 5240 1903 Size: 34 50 34 835 34 2316 34 3678 Fragments arranged by size: 3678 2316 835 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4907 6117 4907 Size: 1210 5805 BclI T'GATC_A Cuts at: 1548 1548 Size: 7015 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2045 5626 136 Size: 107 122 71 631 605 373 3581 1525 Fragments arranged by size: 3581 1525 631 605 373 122 107 71 BglII A'GATC_T Cuts at: 3999 7010 3999 Size: 3011 4004 BlpI GC'TnA_GC Cuts at: 4432 4432 Size: 7015 Bme1580I G_kGCm'C Cuts at: 1790 2956 5076 6232 1790 Size: 1166 2120 1156 2573 Fragments arranged by size: 2573 2120 1166 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7015 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4746 5704 442 Size: 416 53 83 399 24 3329 958 1753 Fragments arranged by size: 3329 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7015 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2635 2893 4018 2635 Size: 258 1125 5632 Fragments arranged by size: 5632 1125 258 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 3443 3842 5675 1239 Size: 839 1365 399 1833 2579 Fragments arranged by size: 2579 1833 1365 839 399 Bpu10I CC'TnA_GC Cuts at: 2709 2778 2709 Size: 69 6946 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3038 3218 5140 6005 6303 3038 Size: 180 1922 865 298 3750 Fragments arranged by size: 3750 1922 865 298 180 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5678 882 Size: 617 4179 2219 Fragments arranged by size: 4179 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3472 4806 493 Size: 711 2268 1334 2702 Fragments arranged by size: 2702 2268 1334 711 BsaBI GATnn'nnATC Cuts at: 2166 4154 7009 2166 Size: 1988 2855 2172 Fragments arranged by size: 2855 2172 1988 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3729 4221 4822 5204 Size: 53 83 186 936 2196 492 601 382 Cuts at: 5204 5839 275 Size: 635 1451 Fragments arranged by size: 2196 1451 936 635 601 492 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2985 3582 5448 5846 6120 6267 6678 2985 Size: 597 1866 398 274 147 411 3322 Fragments arranged by size: 3322 1866 597 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6790 Fragments arranged by size: 6790 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2624 2708 3447 3686 4010 4253 1186 Size: 1438 84 739 239 324 243 3948 Fragments arranged by size: 3948 1438 739 324 243 239 84 BseYI C'CCAG_C Cuts at: 6218 6714 6218 Size: 496 6519 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6442 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3946 4465 5226 6254 1540 Size: 275 186 1945 519 761 1028 2301 Fragments arranged by size: 2301 1945 1028 761 519 275 186 BsiHKAI G_wGCw'C Cuts at: 2879 3822 5076 5161 6232 2879 Size: 943 1254 85 1071 3662 Fragments arranged by size: 3662 1254 1071 943 85 BsmI GAATG_Cn' Cuts at: 3868 4526 4619 3868 Size: 658 93 6264 Fragments arranged by size: 6264 658 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2885 2885 Size: 7015 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2879 2956 3822 4334 5076 5161 6232 Size: 399 1089 77 866 512 742 85 1071 Cuts at: 6232 1391 Size: 2174 Fragments arranged by size: 2174 1089 1071 866 742 512 399 85 77 BspEI T'CCGG_A Cuts at: 6678 6678 Size: 7015 BspHI T'CATG_A Cuts at: 3231 4904 6634 3231 Size: 1673 1730 3612 Fragments arranged by size: 3612 1730 1673 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2195 4370 844 Size: 1351 2175 3489 Fragments arranged by size: 3489 2175 1351 BsrBI CCG'CTC Cuts at: 1394 1823 3440 3575 4902 1394 Size: 429 1617 135 1327 3507 Fragments arranged by size: 3507 1617 1327 429 135 BsrDI GCAATG_nn' Cuts at: 65 1248 2817 2916 5510 5684 65 Size: 1183 1569 99 2594 174 1396 Fragments arranged by size: 2594 1569 1396 1183 174 99 BsrFI r'CCGG_y Cuts at: 1700 1810 5659 5846 1700 Size: 110 3849 187 2869 Fragments arranged by size: 3849 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7015 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7015 BssSI C'ACGA_G Cuts at: 5075 6087 5075 Size: 1012 6003 BstAPI GCAn_nnn'nTGC Cuts at: 794 2316 794 Size: 1522 5493 BstBI TT'CG_AA Cuts at: 5819 5819 Size: 7015 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7015 BstYI r'GATC_y Cuts at: 1972 2167 3082 3559 3579 3999 4345 4712 5097 Size: 195 915 477 20 420 346 367 385 Cuts at: 5097 5114 6566 6652 6664 7010 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 915 477 420 385 367 346 346 195 86 20 17 12 BtgI C'CryG_G Cuts at: 513 1065 3813 3863 513 Size: 552 2748 50 3665 Fragments arranged by size: 3665 2748 552 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6276 Fragments arranged by size: 6276 683 56 BtsI GCAGTG_nn' Cuts at: 2021 2321 2485 3669 4531 5325 5345 2021 Size: 300 164 1184 862 794 20 3691 Fragments arranged by size: 3691 1184 862 794 300 164 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7015 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2800 2835 529 Size: 35 2236 35 4709 Fragments arranged by size: 4709 2236 35 35 DraI TTT'AAA Cuts at: 4319 4319 Size: 7015 DraIII CAC_nnn'GTG Cuts at: 2338 2338 Size: 7015 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2624 4226 6022 809 Size: 507 1308 1602 1796 1802 Fragments arranged by size: 1802 1796 1602 1308 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 3018 3378 Size: 54 1425 50 275 18 204 984 360 Cuts at: 3378 3666 3735 4462 5351 6980 8 Size: 288 69 727 889 1629 43 Fragments arranged by size: 1629 1425 984 889 727 360 288 275 204 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4462 1537 Size: 275 2650 4090 Fragments arranged by size: 4090 2650 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2480 2766 2850 3000 3240 4945 1353 Size: 1127 286 84 150 240 1705 3423 Fragments arranged by size: 3423 1705 1127 286 240 150 84 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2652 2985 5602 5976 6122 387 Size: 1064 138 1063 333 2617 374 146 1280 Fragments arranged by size: 2617 1280 1064 1063 374 333 146 138 EcoICRI GAG'CTC Cuts at: 3820 3820 Size: 7015 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2595 2961 3443 3686 3842 4026 Size: 393 446 517 366 482 243 156 184 Cuts at: 4026 4193 5078 5675 6462 6730 1239 Size: 167 885 597 787 268 1524 Fragments arranged by size: 1524 885 787 597 517 482 446 393 366 268 243 184 167 156 EcoRI G'AATT_C Cuts at: 4025 4324 4702 4025 Size: 299 378 6338 Fragments arranged by size: 6338 378 299 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3212 3212 Size: 7015 FspI TGC'GCA Cuts at: 3551 5521 3551 Size: 1970 5045 FspAI rTGC'GCAy Cuts at: 3551 3551 Size: 7015 HaeII r_GCGC'y Cuts at: 1678 2057 3036 4246 6162 1678 Size: 379 979 1210 1916 2531 Fragments arranged by size: 2531 1916 1210 979 379 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2898 2930 3206 3238 5656 Size: 32 135 32 1326 32 276 32 2418 Cuts at: 5656 5688 5730 5762 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2418 1326 276 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3130 3978 4219 4359 4605 669 Size: 988 281 1192 848 241 140 246 3079 Fragments arranged by size: 3079 1192 988 848 281 246 241 140 HindIII A'AGCT_T Cuts at: 748 748 Size: 7015 HpaI GTT'AAC Cuts at: 4605 4605 Size: 7015 KpnI G_GTAC'C Cuts at: 2066 4340 2066 Size: 2274 4741 MfeI C'AATT_G Cuts at: 4614 4614 Size: 7015 MluI A'CGCG_T Cuts at: 6891 6891 Size: 7015 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4054 4307 6129 6313 1305 Size: 2749 253 1822 184 2007 Fragments arranged by size: 2749 2007 1822 253 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3380 3737 10 Size: 54 1425 343 1548 357 3288 Fragments arranged by size: 3288 1548 1425 357 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2135 2139 2255 3020 3606 4163 4203 Size: 648 969 4 116 765 586 557 40 Cuts at: 4203 4973 5332 5491 518 Size: 770 359 159 2042 Fragments arranged by size: 2042 969 770 765 648 586 557 359 159 116 40 4 MspA1I CmG'CkG Cuts at: 1606 2352 2922 3361 4226 4431 5108 6256 6501 Size: 746 570 439 865 205 677 1148 245 Cuts at: 6501 1606 Size: 2120 Fragments arranged by size: 2120 1148 865 746 677 570 439 245 205 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6463 NdeI CA'TA_TG Cuts at: 387 387 Size: 7015 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7015 NotI GC'GGCC_GC Cuts at: 4462 4462 Size: 7015 NspI r_CATG'y Cuts at: 1132 1229 2134 2478 2515 3170 3548 4373 5918 Size: 97 905 344 37 655 378 825 1545 Cuts at: 5918 1132 Size: 2229 Fragments arranged by size: 2229 1545 905 825 655 378 344 97 37 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7015 PciI A'CATG_T Cuts at: 5914 5914 Size: 7015 PflMI CCAn_nnn'nTGG Cuts at: 1108 3971 1108 Size: 2863 4152 PfoI T'CCnGG_A Cuts at: 3309 3309 Size: 7015 PmeI GTTT'AAAC Cuts at: 4319 4319 Size: 7015 PmlI CAC'GTG Cuts at: 3472 3472 Size: 7015 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5084 5116 6597 6629 5084 Size: 32 1481 32 5470 Fragments arranged by size: 5470 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7015 PsiI TTA'TAA Cuts at: 4585 4585 Size: 7015 PstI C_TGCA'G Cuts at: 830 2217 2247 3447 3664 4367 830 Size: 1387 30 1200 217 703 3478 Fragments arranged by size: 3478 1387 1200 703 217 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7015 PvuII CAG'CTG Cuts at: 3361 3361 Size: 7015 SacI G_AGCT'C Cuts at: 3822 3822 Size: 7015 SalI G'TCGA_C Cuts at: 1936 4357 1936 Size: 2421 4594 SbfI CC_TGCA'GG Cuts at: 4367 4367 Size: 7015 ScaI AGT'ACT Cuts at: 3011 5263 6975 3011 Size: 2252 1712 3051 Fragments arranged by size: 3051 2252 1712 SexAI A'CCwGG_T Cuts at: 3964 3964 Size: 7015 SfcI C'TryA_G Cuts at: 826 1045 2213 2243 3443 3660 4363 5498 6179 Size: 219 1168 30 1200 217 703 1135 681 Cuts at: 6179 6370 826 Size: 191 1471 Fragments arranged by size: 1471 1200 1168 1135 703 681 219 217 191 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7015 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7015 SmaI CCC'GGG Cuts at: 1872 4342 1872 Size: 2470 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3053 3140 3197 5119 6020 6282 Size: 197 926 1110 87 57 1922 901 262 Cuts at: 6282 820 Size: 1553 Fragments arranged by size: 1922 1553 1110 926 901 262 197 87 57 SnaBI TAC'GTA Cuts at: 493 4806 493 Size: 4313 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7015 SphI G_CATG'C Cuts at: 1132 4373 1132 Size: 3241 3774 SspI AAT'ATT Cuts at: 5 52 4939 5 Size: 47 4887 2081 Fragments arranged by size: 4887 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7015 StyI C'CwwG_G Cuts at: 513 1065 2199 3755 4161 4454 513 Size: 552 1134 1556 406 293 3074 Fragments arranged by size: 3074 1556 1134 552 406 293 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3812 3960 5042 5059 5212 5397 5842 Size: 1255 2232 148 1082 17 153 185 445 Cuts at: 5842 325 Size: 1498 Fragments arranged by size: 2232 1498 1255 1082 445 185 153 148 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3009 4185 5261 Size: 274 80 33 51 1443 1032 1176 1076 Cuts at: 5261 6973 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1176 1076 1032 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2615 2647 4107 4139 6975 7007 2615 Size: 32 1460 32 2836 32 2623 Fragments arranged by size: 2836 2623 1460 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7015 XbaI T'CTAG_A Cuts at: 4351 4351 Size: 7015 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2848 2848 Size: 7015 XhoI C'TCGA_G Cuts at: 1943 3140 1943 Size: 1197 5818 XmaI C'CCGG_G Cuts at: 1870 4340 1870 Size: 2470 4545 XmnI GAAnn'nnTTC Cuts at: 5144 5144 Size: 7015 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3730 4823 276 Size: 53 83 186 936 2196 1093 2468 Fragments arranged by size: 2468 2196 1093 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI