Restriction map for
pFN21AB3334
(Linear) (Six-base) MAPSORT of: KIBB3334.seq Check: 4606 from: 1 to: 2310
KIBB3334.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AatII G_ACGT'C
Cuts at: 0 1727 2310
Size: 1727 583
AccI GT'mk_AC
Cuts at: 0 227 2310
Size: 227 2083
Acc65I G'GTAC_C
Cuts at: 0 57 2310
Size: 57 2253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 590 956 1681 2021 2188 2310
Size: 590 366 725 340 167 122
Fragments arranged by size:
725 590 366 340 167 122
AflIII A'CryG_T
Cuts at: 0 1466 2310
Size: 1466 844
AleI CACnn'nnGTG
Cuts at: 0 250 1601 2158 2310
Size: 250 1351 557 152
Fragments arranged by size:
1351 557 250 152
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 529 2310
Size: 529 1781
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2030 2062 2310
Size: 2030 32 248
Fragments arranged by size:
2030 248 32
AlwNI CAG_nnn'CTG
Cuts at: 0 844 1356 1966 2310
Size: 844 512 610 344
Fragments arranged by size:
844 610 512 344
ApoI r'AATT_y
Cuts at: 0 400 2020 2310
Size: 400 1620 290
Fragments arranged by size:
1620 400 290
AvaI C'yCGr_G
Cuts at: 0 44 1073 1135 2310
Size: 44 1029 62 1175
Fragments arranged by size:
1175 1029 62 44
BamHI G'GATC_C
Cuts at: 0 1554 2310
Size: 1554 756
BanI G'GyrC_C
Cuts at: 0 57 2310
Size: 57 2253
BanII G_rGCy'C
Cuts at: 0 1817 2310
Size: 1817 493
BbsI GAAGACnn'nnnn_
Cuts at: 0 671 2310
Size: 671 1639
BbvCI CC'TCA_GC
Cuts at: 0 704 773 2310
Size: 704 69 1537
Fragments arranged by size:
1537 704 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 851 885 2310
Size: 851 34 1425
Fragments arranged by size:
1425 851 34
BglI GCCn_nnn'nGGC
Cuts at: 0 40 2310
Size: 40 2270
BglII A'GATC_T
Cuts at: 0 1994 2310
Size: 1994 316
Bme1580I G_kGCm'C
Cuts at: 0 951 2310
Size: 951 1359
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 630 888 2013 2310
Size: 630 258 1125 297
Fragments arranged by size:
1125 630 297 258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 1438 1837 2310
Size: 73 1365 399 473
Fragments arranged by size:
1365 473 399 73
Bpu10I CC'TnA_GC
Cuts at: 0 704 773 2310
Size: 704 69 1537
Fragments arranged by size:
1537 704 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1213 2310
Size: 1033 180 1097
Fragments arranged by size:
1097 1033 180
BsaAI yAC'GTr
Cuts at: 0 1467 2310
Size: 1467 843
BsaBI GATnn'nnATC
Cuts at: 0 161 2149 2310
Size: 161 1988 161
Fragments arranged by size:
1988 161 161
BsaHI Gr'CG_yC
Cuts at: 0 1724 2216 2310
Size: 1724 492 94
Fragments arranged by size:
1724 492 94
BsaWI w'CCGG_w
Cuts at: 0 980 1577 2310
Size: 980 597 733
Fragments arranged by size:
980 733 597
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 619 703 1442 1681 2005 2248 2310
Size: 619 84 739 239 324 243 62
Fragments arranged by size:
739 619 324 243 239 84 62
BsiEI CG_ry'CG
Cuts at: 0 1941 2310
Size: 1941 369
BsiHKAI G_wGCw'C
Cuts at: 0 874 1817 2310
Size: 874 943 493
Fragments arranged by size:
943 874 493
BsmI GAATG_Cn'
Cuts at: 0 1863 2310
Size: 1863 447
BsmBI CGTCTCn'nnnn_
Cuts at: 0 880 2310
Size: 880 1430
Bsp1286I G_dGCh'C
Cuts at: 0 874 951 1817 2310
Size: 874 77 866 493
Fragments arranged by size:
874 866 493 77
BspHI T'CATG_A
Cuts at: 0 1226 2310
Size: 1226 1084
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 190 2310
Size: 190 2120
BsrBI CCG'CTC
Cuts at: 0 1435 1570 2310
Size: 1435 135 740
Fragments arranged by size:
1435 740 135
BsrDI GCAATG_nn'
Cuts at: 0 812 911 2310
Size: 812 99 1399
Fragments arranged by size:
1399 812 99
BstAPI GCAn_nnn'nTGC
Cuts at: 0 311 2310
Size: 311 1999
BstYI r'GATC_y
Cuts at: 0 162 1077 1554 1574 1994 2310
Size: 162 915 477 20 420 316
Fragments arranged by size:
915 477 420 316 162 20
BtgI C'CryG_G
Cuts at: 0 1808 1858 2310
Size: 1808 50 452
Fragments arranged by size:
1808 452 50
BtsI GCAGTG_nn'
Cuts at: 0 16 316 480 1664 2310
Size: 16 300 164 1184 646
Fragments arranged by size:
1184 646 300 164 16
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 795 830 2310
Size: 795 35 1480
Fragments arranged by size:
1480 795 35
DraIII CAC_nnn'GTG
Cuts at: 0 333 2310
Size: 333 1977
DrdI GACnn_nn'nnGTC
Cuts at: 0 619 2221 2310
Size: 619 1602 89
Fragments arranged by size:
1602 619 89
EaeI y'GGCC_r
Cuts at: 0 29 1013 1373 1661 1730 2310
Size: 29 984 360 288 69 580
Fragments arranged by size:
984 580 360 288 69 29
EarI CTCTTCn'nnn_
Cuts at: 0 475 761 845 995 1235 2310
Size: 475 286 84 150 240 1075
Fragments arranged by size:
1075 475 286 240 150 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 647 980 2310
Size: 647 333 1330
Fragments arranged by size:
1330 647 333
EcoICRI GAG'CTC
Cuts at: 0 1815 2310
Size: 1815 495
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 590 956 1438 1681 1837 2021 2188
Size: 73 517 366 482 243 156 184 167
Cuts at: 2188 2310
Size: 122
Fragments arranged by size:
517 482 366 243 184 167 156 122
73
EcoRI G'AATT_C
Cuts at: 0 2020 2310
Size: 2020 290
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1207 2310
Size: 1207 1103
FspI TGC'GCA
Cuts at: 0 1546 2310
Size: 1546 764
FspAI rTGC'GCAy
Cuts at: 0 1546 2310
Size: 1546 764
HaeII r_GCGC'y
Cuts at: 0 52 1031 2241 2310
Size: 52 979 1210 69
Fragments arranged by size:
1210 979 69 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 893 925 1201 1233 2310
Size: 893 32 276 32 1077
Fragments arranged by size:
1077 893 276 32 32
HincII GTy'rAC
Cuts at: 0 1125 1973 2214 2310
Size: 1125 848 241 96
Fragments arranged by size:
1125 848 241 96
KpnI G_GTAC'C
Cuts at: 0 61 2310
Size: 61 2249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2049 2302 2310
Size: 2049 253 8
Fragments arranged by size:
2049 253 8
MscI TGG'CCA
Cuts at: 0 1375 1732 2310
Size: 1375 357 578
Fragments arranged by size:
1375 578 357
MslI CAynn'nnrTG
Cuts at: 0 130 134 250 1015 1601 2158 2198 2310
Size: 130 4 116 765 586 557 40 112
Fragments arranged by size:
765 586 557 130 116 112 40 4
MspA1I CmG'CkG
Cuts at: 0 347 917 1356 2221 2310
Size: 347 570 439 865 89
Fragments arranged by size:
865 570 439 347 89
NspI r_CATG'y
Cuts at: 0 129 473 510 1165 1543 2310
Size: 129 344 37 655 378 767
Fragments arranged by size:
767 655 378 344 129 37
PflMI CCAn_nnn'nTGG
Cuts at: 0 1966 2310
Size: 1966 344
PfoI T'CCnGG_A
Cuts at: 0 1304 2310
Size: 1304 1006
PmlI CAC'GTG
Cuts at: 0 1467 2310
Size: 1467 843
PstI C_TGCA'G
Cuts at: 0 212 242 1442 1659 2310
Size: 212 30 1200 217 651
Fragments arranged by size:
1200 651 217 212 30
PvuII CAG'CTG
Cuts at: 0 1356 2310
Size: 1356 954
SacI G_AGCT'C
Cuts at: 0 1817 2310
Size: 1817 493
ScaI AGT'ACT
Cuts at: 0 1006 2310
Size: 1006 1304
SexAI A'CCwGG_T
Cuts at: 0 1959 2310
Size: 1959 351
SfcI C'TryA_G
Cuts at: 0 208 238 1438 1655 2310
Size: 208 30 1200 217 655
Fragments arranged by size:
1200 655 217 208 30
SmlI C'TyrA_G
Cuts at: 0 1048 1135 1192 2310
Size: 1048 87 57 1118
Fragments arranged by size:
1118 1048 87 57
StyI C'CwwG_G
Cuts at: 0 194 1750 2156 2310
Size: 194 1556 406 154
Fragments arranged by size:
1556 406 194 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1807 1955 2310
Size: 1807 148 355
Fragments arranged by size:
1807 355 148
TatI w'GTAC_w
Cuts at: 0 1004 2180 2310
Size: 1004 1176 130
Fragments arranged by size:
1176 1004 130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 610 642 2102 2134 2310
Size: 610 32 1460 32 176
Fragments arranged by size:
1460 610 176 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 843 2310
Size: 843 1467
XhoI C'TCGA_G
Cuts at: 0 1135 2310
Size: 1135 1175
ZraI GAC'GTC
Cuts at: 0 1725 2310
Size: 1725 585
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AleI AlfI AloI
AlwNI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BglI BglII Bme1580I BplI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BstAPI BstYI
BtgI BtsI CspCI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI FspAI HaeII Hin4I
HincII KpnI MmeI MscI MslI MspA1I NspI PflMI
PfoI PmlI PstI PvuII SacI ScaI SexAI SfcI
SmlI StyI TaqII TatI TstI XcmI XhoI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI
BfrBI BlpI BmgBI BmrI BmtI BsaI BsaXI BseYI
BsgI BsiWI BspEI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI EagI
EcoNI EcoO109I EcoRV FseI HindIII HpaI KasI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XmaI XmnI