(Circular) (Six-base) MAPSORT of: pFN21AB3332.seq Check: 215 from: 1 to: 8095 pFN21AB3332 8095 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2404 3396 3944 3955 5260 2404 Size: 992 548 11 1305 5239 Fragments arranged by size: 5239 1305 992 548 11 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5905 278 Size: 53 83 186 936 4369 2468 Fragments arranged by size: 4369 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5438 1937 Size: 3501 4594 Acc65I G'GTAC_C Cuts at: 5416 5416 Size: 8095 AclI AA'CG_TT Cuts at: 1563 6222 6595 1563 Size: 4659 373 3063 Fragments arranged by size: 4659 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2786 3335 3967 4041 4512 4611 5109 6158 Size: 1154 549 632 74 471 99 498 1049 Cuts at: 6158 7542 7810 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1154 1049 632 549 498 471 268 99 74 AfeI AGC'GCT Cuts at: 5094 5094 Size: 8095 AflII C'TTAA_G Cuts at: 820 1017 3422 820 Size: 197 2405 5493 Fragments arranged by size: 5493 2405 197 AflIII A'CryG_T Cuts at: 1203 3564 6994 7971 1203 Size: 2361 3430 977 1327 Fragments arranged by size: 3430 2361 1327 977 AgeI A'CCGG_T Cuts at: 6926 6926 Size: 8095 AhdI GACnn_n'nnGTC Cuts at: 2070 3142 6824 2070 Size: 1072 3682 3341 Fragments arranged by size: 3682 3341 1072 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8095 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8095 AlwNI CAG_nnn'CTG Cuts at: 2855 4085 5387 5838 7410 2855 Size: 1230 1302 451 1572 3540 Fragments arranged by size: 3540 1572 1302 1230 451 ApaI G_GGCC'C Cuts at: 2031 2096 2251 2836 3932 3991 4013 5148 2031 Size: 65 155 585 1096 59 22 1135 4978 Fragments arranged by size: 4978 1135 1096 585 155 65 59 22 ApaLI G'TGCA_C Cuts at: 4929 6152 7308 4929 Size: 1223 1156 5716 Fragments arranged by size: 5716 1223 1156 ApoI r'AATT_y Cuts at: 1493 4805 5404 5630 5782 7891 1493 Size: 3312 599 226 152 2109 1697 Fragments arranged by size: 3312 2109 1697 599 226 152 AseI AT'TA_AT Cuts at: 160 1032 4647 6649 160 Size: 872 3615 2002 1606 Fragments arranged by size: 3615 2002 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8095 AvaI C'yCGr_G Cuts at: 1870 1943 2089 2204 2625 3903 4379 5012 5420 Size: 73 146 115 421 1278 476 633 408 Cuts at: 5420 1870 Size: 4545 Fragments arranged by size: 4545 1278 633 476 421 408 146 115 73 AvrII C'CTAG_G Cuts at: 2968 3697 4129 2968 Size: 729 432 6934 Fragments arranged by size: 6934 729 432 BamHI G'GATC_C Cuts at: 3189 5425 5792 3189 Size: 2236 367 5492 Fragments arranged by size: 5492 2236 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4418 5416 6871 618 Size: 325 217 627 2631 998 1455 1842 Fragments arranged by size: 2631 1842 1455 998 627 325 217 BanII G_rGCy'C Cuts at: 1391 2031 2042 2090 2096 2211 2251 2363 2693 Size: 640 11 48 6 115 40 112 330 Cuts at: 2693 2836 2976 3001 3449 3722 3932 3991 4013 Size: 143 140 25 448 273 210 59 22 Cuts at: 4013 4031 5148 5178 5414 1391 Size: 18 1117 30 236 4072 Fragments arranged by size: 4072 1117 640 448 330 273 236 210 143 140 115 112 59 48 40 30 25 22 18 11 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4734 4893 928 Size: 971 2835 159 4130 Fragments arranged by size: 4130 2835 971 159 BbvCI CC'TCA_GC Cuts at: 2694 3050 3590 4039 4075 2694 Size: 356 540 449 36 6714 Fragments arranged by size: 6714 540 449 356 36 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2070 2104 3551 3585 6286 6320 1903 Size: 34 133 34 1447 34 2701 34 3678 Fragments arranged by size: 3678 2701 1447 133 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5987 7197 5987 Size: 1210 6885 BclI T'GATC_A Cuts at: 1548 1548 Size: 8095 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4384 5330 Size: 107 122 71 631 605 337 2375 946 Cuts at: 5330 6706 136 Size: 1376 1525 Fragments arranged by size: 2375 1525 1376 946 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 4335 8090 4335 Size: 3755 4340 BlpI GC'TnA_GC Cuts at: 2981 3392 5512 2981 Size: 411 2120 5564 Fragments arranged by size: 5564 2120 411 Bme1580I G_kGCm'C Cuts at: 1790 2031 2096 2251 2836 3808 3932 3991 4013 Size: 241 65 155 585 972 124 59 22 Cuts at: 4013 4092 4287 4380 4933 5148 6156 7312 1790 Size: 79 195 93 553 215 1008 1156 2573 Fragments arranged by size: 2573 1156 1008 972 585 553 241 215 195 155 124 93 79 65 59 22 BmgBI CAC'GTC Cuts at: 1318 2297 3565 1318 Size: 979 1268 5848 Fragments arranged by size: 5848 1268 979 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3477 4455 4573 Size: 416 53 83 399 24 2060 978 118 Cuts at: 4573 5826 6784 442 Size: 1253 958 1753 Fragments arranged by size: 2060 1753 1253 978 958 416 399 118 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8095 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3266 3871 5407 3266 Size: 605 1536 5954 Fragments arranged by size: 5954 1536 605 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2915 3240 3250 3387 3936 4913 4938 Size: 899 777 325 10 137 549 977 25 Cuts at: 4938 5279 6755 1239 Size: 341 1476 2579 Fragments arranged by size: 2579 1476 977 899 777 549 341 325 137 25 10 Bpu10I CC'TnA_GC Cuts at: 2694 3050 3590 4039 4075 2694 Size: 356 540 449 36 6714 Fragments arranged by size: 6714 540 449 356 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2707 3146 3866 6220 7085 7383 2707 Size: 439 720 2354 865 298 3419 Fragments arranged by size: 3419 2354 865 720 439 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2078 3856 6758 882 Size: 617 579 1778 2902 2219 Fragments arranged by size: 2902 2219 1778 617 579 BsaAI yAC'GTr Cuts at: 493 1204 5886 493 Size: 711 4682 2702 Fragments arranged by size: 4682 2702 711 BsaBI GATnn'nnATC Cuts at: 3194 8089 3194 Size: 4895 3200 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5902 6284 6919 275 Size: 53 83 186 936 4369 382 635 1451 Fragments arranged by size: 4369 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2167 3534 6528 6926 7200 7347 7758 2167 Size: 1367 2994 398 274 147 411 2504 Fragments arranged by size: 2994 2504 1367 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2988 3018 3223 3253 3704 Size: 30 165 30 1585 30 205 30 451 Cuts at: 3704 3734 3934 3964 4555 4585 4902 4932 4936 Size: 30 200 30 591 30 317 30 4 Cuts at: 4936 4966 1178 Size: 30 4307 Fragments arranged by size: 4307 1585 591 451 317 205 200 165 30 30 30 30 30 30 30 30 30 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2468 3372 3644 3768 3783 3844 4331 4499 Size: 1282 904 272 124 15 61 487 168 Cuts at: 4499 4685 4688 4736 4796 4862 4871 4874 5048 Size: 186 3 48 60 66 9 3 174 Cuts at: 5048 5063 5066 5219 1186 Size: 15 3 153 4062 Fragments arranged by size: 4062 1282 904 487 272 186 174 168 153 124 66 61 60 48 15 15 9 3 3 3 BseYI C'CCAG_C Cuts at: 2048 2493 2557 2781 2827 4618 7298 7794 2048 Size: 445 64 224 46 1791 2680 496 2349 Fragments arranged by size: 2680 2349 1791 496 445 224 64 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2487 2801 2885 3038 3132 3341 3561 Size: 573 751 314 84 153 94 209 220 Cuts at: 3561 5009 5252 1163 Size: 1448 243 4006 Fragments arranged by size: 4006 1448 751 573 314 243 220 209 153 94 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3202 5545 6306 7334 1540 Size: 275 186 1201 2343 761 1028 2301 Fragments arranged by size: 2343 2301 1201 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2090 3001 3722 4933 6156 6241 7312 2090 Size: 911 721 1211 1223 85 1071 2873 Fragments arranged by size: 2873 1223 1211 1071 911 721 85 BsmI GAATG_Cn' Cuts at: 2406 3351 5606 5699 2406 Size: 945 2255 93 4802 Fragments arranged by size: 4802 2255 945 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2303 3780 2303 Size: 1477 6618 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2042 2090 2096 2211 2251 2363 Size: 399 241 11 48 6 115 40 112 Cuts at: 2363 2693 2836 2976 3001 3449 3722 3808 3932 Size: 330 143 140 25 448 273 86 124 Cuts at: 3932 3991 4013 4031 4092 4287 4380 4933 5148 Size: 59 22 18 61 195 93 553 215 Cuts at: 5148 5178 5414 6156 6241 7312 1391 Size: 30 236 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 553 448 399 330 273 241 236 215 195 143 140 124 115 112 93 86 85 61 59 48 40 30 25 22 18 11 6 BspEI T'CCGG_A Cuts at: 7758 7758 Size: 8095 BspHI T'CATG_A Cuts at: 5984 7714 5984 Size: 1730 6365 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2404 3147 3313 3396 3944 3955 5260 5450 Size: 1560 743 166 83 548 11 1305 190 Cuts at: 5450 844 Size: 3489 Fragments arranged by size: 3489 1560 1305 743 548 190 166 83 11 BsrBI CCG'CTC Cuts at: 1394 1823 2187 5982 1394 Size: 429 364 3795 3507 Fragments arranged by size: 3795 3507 429 364 BsrDI GCAATG_nn' Cuts at: 65 1248 3556 6590 6764 65 Size: 1183 2308 3034 174 1396 Fragments arranged by size: 3034 2308 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3171 6739 6926 1700 Size: 110 1361 3568 187 2869 Fragments arranged by size: 3568 2869 1361 187 110 BsrGI T'GTAC_A Cuts at: 96 3679 96 Size: 3583 4512 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8095 BssSI C'ACGA_G Cuts at: 3715 6155 7167 3715 Size: 2440 1012 4643 Fragments arranged by size: 4643 2440 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2824 2855 794 Size: 2030 31 6034 Fragments arranged by size: 6034 2030 31 BstBI TT'CG_AA Cuts at: 6899 6899 Size: 8095 BstEII G'GTnAC_C Cuts at: 1184 3877 1184 Size: 2693 5402 BstXI CCAn_nnnn'nTGG Cuts at: 2537 2537 Size: 8095 BstYI r'GATC_y Cuts at: 1972 3189 4335 5425 5792 6177 6194 7646 7732 Size: 1217 1146 1090 367 385 17 1452 86 Cuts at: 7732 7744 8090 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1217 1146 1090 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2468 2564 3966 4414 2468 Size: 96 1402 448 6149 Fragments arranged by size: 6149 1402 448 96 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 2096 2457 3437 3924 513 Size: 552 939 39 53 361 980 487 4684 Fragments arranged by size: 4684 980 939 552 487 361 53 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3960 487 Size: 683 56 2734 4622 Fragments arranged by size: 4622 2734 683 56 BtsI GCAGTG_nn' Cuts at: 2088 2813 4666 5611 6405 6425 2088 Size: 725 1853 945 794 20 3758 Fragments arranged by size: 3758 1853 945 794 725 20 ClaI AT'CG_AT Cuts at: 1554 5343 1554 Size: 3789 4306 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4563 4598 529 Size: 35 3999 35 4026 Fragments arranged by size: 4026 3999 35 35 DraI TTT'AAA Cuts at: 5399 5399 Size: 8095 DraIII CAC_nnn'GTG Cuts at: 2389 2389 Size: 8095 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7102 809 Size: 507 5786 1802 Fragments arranged by size: 5786 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2454 3199 3826 Size: 54 1425 50 275 18 624 745 627 Cuts at: 3826 5331 5542 6431 8060 8 Size: 1505 211 889 1629 43 Fragments arranged by size: 1629 1505 1425 889 745 627 624 275 211 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3199 5542 1537 Size: 275 1387 2343 4090 Fragments arranged by size: 4090 2343 1387 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4623 4662 4674 4683 4698 4707 4713 4734 Size: 3270 39 12 9 15 9 6 21 Cuts at: 4734 4740 4749 4764 4773 4785 4839 4872 5166 Size: 6 9 15 9 12 54 33 294 Cuts at: 5166 5196 5256 6025 1353 Size: 30 60 769 3423 Fragments arranged by size: 3423 3270 769 294 60 54 39 33 30 21 15 15 12 12 9 9 9 9 6 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2093 4527 6682 7056 7202 387 Size: 1064 138 504 2434 2155 374 146 1280 Fragments arranged by size: 2434 2155 1280 1064 504 374 146 138 EcoICRI GAG'CTC Cuts at: 2088 2999 3720 2088 Size: 911 721 6463 Fragments arranged by size: 6463 911 721 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2786 2915 3240 3250 3335 3387 Size: 393 506 648 129 325 10 85 52 Cuts at: 3387 3936 3967 4041 4512 4611 4913 4938 5109 Size: 549 31 74 471 99 302 25 171 Cuts at: 5109 5279 6158 6755 7542 7810 1239 Size: 170 879 597 787 268 1524 Fragments arranged by size: 1524 879 787 648 597 549 506 471 393 325 302 268 171 170 129 99 85 74 52 31 25 10 EcoNI CCTnn'n_nnAGG Cuts at: 2840 4059 4628 5139 2840 Size: 1219 569 511 5796 Fragments arranged by size: 5796 1219 569 511 EcoO109I rG'GnC_Cy Cuts at: 2028 2247 2326 2431 2473 2832 2833 2876 3217 Size: 219 79 105 42 359 1 43 341 Cuts at: 3217 3333 3373 3694 3929 3970 3987 3988 4010 Size: 116 40 321 235 41 17 1 22 Cuts at: 4010 4046 4064 4082 4213 4514 4613 5144 5145 Size: 36 18 18 131 301 99 531 1 Cuts at: 5145 2028 Size: 4978 Fragments arranged by size: 4978 531 359 341 321 301 235 219 131 116 105 99 79 43 42 41 40 36 22 18 18 17 1 1 1 EcoRI G'AATT_C Cuts at: 5404 5782 5404 Size: 378 7717 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3860 3860 Size: 8095 FspI TGC'GCA Cuts at: 6601 6601 Size: 8095 HaeII r_GCGC'y Cuts at: 1678 3483 3761 5096 7242 1678 Size: 1805 278 1335 2146 2531 Fragments arranged by size: 2531 2146 1805 1335 278 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2056 2088 3128 3160 3236 Size: 32 135 32 484 32 1040 32 76 Cuts at: 3236 3268 3959 3991 4307 4339 4555 4587 6736 Size: 32 691 32 316 32 216 32 2149 Cuts at: 6736 6768 6810 6842 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2149 1040 691 484 316 216 135 76 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5439 5685 669 Size: 988 281 3501 246 3079 Fragments arranged by size: 3501 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8095 HpaI GTT'AAC Cuts at: 5685 5685 Size: 8095 KpnI G_GTAC'C Cuts at: 5420 5420 Size: 8095 MfeI C'AATT_G Cuts at: 5694 5694 Size: 8095 MluI A'CGCG_T Cuts at: 7971 7971 Size: 8095 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2725 5178 7209 7393 1305 Size: 1420 2453 2031 184 2007 Fragments arranged by size: 2453 2031 2007 1420 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6273 Fragments arranged by size: 6273 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2522 3084 3605 6053 6412 6571 518 Size: 648 1356 562 521 2448 359 159 2042 Fragments arranged by size: 2448 2042 1356 648 562 521 359 159 MspA1I CmG'CkG Cuts at: 1606 2045 2098 2497 2964 3413 3439 3726 5321 Size: 439 53 399 467 449 26 287 1595 Cuts at: 5321 5511 6188 7336 7581 1606 Size: 190 677 1148 245 2120 Fragments arranged by size: 2120 1595 1148 677 467 449 439 399 287 245 190 53 26 NaeI GCC'GGC Cuts at: 3173 3173 Size: 8095 NcoI C'CATG_G Cuts at: 513 1065 2004 3924 513 Size: 552 939 1920 4684 Fragments arranged by size: 4684 1920 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8095 NgoMIV G'CCGG_C Cuts at: 3171 3171 Size: 8095 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8095 NotI GC'GGCC_GC Cuts at: 5542 5542 Size: 8095 NspI r_CATG'y Cuts at: 1132 1229 5453 6998 1132 Size: 97 4224 1545 2229 Fragments arranged by size: 4224 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2879 4511 5104 1348 Size: 1531 1632 593 4339 Fragments arranged by size: 4339 1632 1531 593 PciI A'CATG_T Cuts at: 6994 6994 Size: 8095 PflMI CCAn_nnn'nTGG Cuts at: 1108 2707 1108 Size: 1599 6496 PfoI T'CCnGG_A Cuts at: 2511 3027 3227 5295 2511 Size: 516 200 2068 5311 Fragments arranged by size: 5311 2068 516 200 PmeI GTTT'AAAC Cuts at: 5399 5399 Size: 8095 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6164 6196 7677 7709 6164 Size: 32 1481 32 6550 Fragments arranged by size: 6550 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2431 2473 3217 3694 4213 4613 2431 Size: 42 744 477 519 400 5913 Fragments arranged by size: 5913 744 519 477 400 42 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8095 PsiI TTA'TAA Cuts at: 5665 5665 Size: 8095 PspOMI G'GGCC_C Cuts at: 2027 2092 2247 2832 3928 3987 4009 5144 2027 Size: 65 155 585 1096 59 22 1135 4978 Fragments arranged by size: 4978 1135 1096 585 155 65 59 22 PstI C_TGCA'G Cuts at: 830 3921 4507 5257 5447 830 Size: 3091 586 750 190 3478 Fragments arranged by size: 3478 3091 750 586 190 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8095 PvuII CAG'CTG Cuts at: 2497 3413 5321 2497 Size: 916 1908 5271 Fragments arranged by size: 5271 1908 916 SacI G_AGCT'C Cuts at: 2090 3001 3722 2090 Size: 911 721 6463 Fragments arranged by size: 6463 911 721 SacII CC_GC'GG Cuts at: 2046 2099 3440 2046 Size: 53 1341 6701 Fragments arranged by size: 6701 1341 53 SalI G'TCGA_C Cuts at: 1936 5437 1936 Size: 3501 4594 SapI GCTCTTCn'nnn_ Cuts at: 5166 5166 Size: 8095 SbfI CC_TGCA'GG Cuts at: 5447 5447 Size: 8095 ScaI AGT'ACT Cuts at: 6343 8055 6343 Size: 1712 6383 SexAI A'CCwGG_T Cuts at: 3654 3654 Size: 8095 SfcI C'TryA_G Cuts at: 826 1045 3917 4434 4503 5253 5443 6578 7259 Size: 219 2872 517 69 750 190 1135 681 Cuts at: 7259 7450 826 Size: 191 1471 Fragments arranged by size: 2872 1471 1135 750 681 517 219 191 190 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8095 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8095 SmaI CCC'GGG Cuts at: 1872 2206 3905 5014 5422 1872 Size: 334 1699 1109 408 4545 Fragments arranged by size: 4545 1699 1109 408 334 SmlI C'TyrA_G Cuts at: 820 1017 1943 2686 3125 3422 3845 6199 7100 Size: 197 926 743 439 297 423 2354 901 Cuts at: 7100 7362 820 Size: 262 1553 Fragments arranged by size: 2354 1553 926 901 743 439 423 297 262 197 SnaBI TAC'GTA Cuts at: 493 5886 493 Size: 5393 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8095 SphI G_CATG'C Cuts at: 1132 5453 1132 Size: 4321 3774 SrfI GCCC'GGGC Cuts at: 2206 2206 Size: 8095 SspI AAT'ATT Cuts at: 5 52 3070 4763 6019 5 Size: 47 3018 1693 1256 2081 Fragments arranged by size: 3018 2081 1693 1256 47 StuI AGG'CCT Cuts at: 1517 2763 3131 3271 3588 4134 4149 1517 Size: 1246 368 140 317 546 15 5463 Fragments arranged by size: 5463 1246 546 368 317 140 15 StyI C'CwwG_G Cuts at: 513 1065 2004 2758 2968 3132 3291 3697 3924 Size: 552 939 754 210 164 159 406 227 Cuts at: 3924 4129 4341 5534 513 Size: 205 212 1193 3074 Fragments arranged by size: 3074 1193 939 754 552 406 227 212 210 205 164 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3307 3398 3665 6122 6139 6292 6477 Size: 1255 1727 91 267 2457 17 153 185 Cuts at: 6477 6922 325 Size: 445 1498 Fragments arranged by size: 2457 1727 1498 1255 445 267 185 153 91 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3679 6341 8053 Size: 274 80 33 51 1443 1702 2662 1712 Cuts at: 8053 96 Size: 138 Fragments arranged by size: 2662 1712 1702 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2988 3020 3934 3966 4588 4620 8055 8087 2988 Size: 32 914 32 622 32 3435 32 2996 Fragments arranged by size: 3435 2996 914 622 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3656 1316 Size: 2340 5755 XbaI T'CTAG_A Cuts at: 5431 5431 Size: 8095 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2449 4021 2449 Size: 1572 6523 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8095 XmaI C'CCGG_G Cuts at: 1870 2204 3903 5012 5420 1870 Size: 334 1699 1109 408 4545 Fragments arranged by size: 4545 1699 1109 408 334 XmnI GAAnn'nnTTC Cuts at: 4497 6224 4497 Size: 1727 6368 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5903 276 Size: 53 83 186 936 4369 2468 Fragments arranged by size: 4369 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SfiI SfoI SwaI