Restriction map for
pFN21AB3329
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII Acc65I AclI AflII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbvCI BcgI
BfrI BglI BglII BlnI BsaBI
BsiWI BspDI BspHI BspLU11I BstBI
Bsu36I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
EcoT14I EcoT22I FseI FspI HindIII
KpnI MamI MfeI MunI NcoI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
SexAI SfiI SfuI SmaI SmlI
SnaBI SpeI Sse8387I SspI StyI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 2148 GTATAC GT^[AC][GT]AC
AccIII 615 TCCGGA T^CCGGA
ApaI 2283 GGGCCC GGGCC^C
AvaI 1369 CCCGAG C^[CT]CG[GA]G
BalI 2512 TGGCCA TGG^CCA
BbrPI 1762 CACGTG CAC^GTG
BlpI 1084 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1084 GCTCAGC GC^T[ACGT]AGC
BsaI 2758 GAGACC GGTCTC(1/5)
BseAI 615 TCCGGA T^CCGGA
BsoBI 1369 CCCGAG C^[CT]CG[GA]G
BspEI 615 TCCGGA T^CCGGA
BsrDI 1101 CATTGC GCAATG(2/0)
BssSI 1570 CACGAG CACGAG(-5/-1)
Bst1107I 2148 GTATAC GTA^TAC
BstAPI 1783 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1149 GGTGACC G^GT[ACGT]ACC
BstPI 1149 GGTGACC G^GT[ACGT]ACC
BstYI 2671 GGATCT [GA]^GATC[CT]
BstZ17I 2148 GTATAC GTA^TAC
CelII 1084 GCTCAGC GC^T[ACGT]AGC
DraIII 1762 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1149 GGTGACC G^GT[ACGT]ACC
HpaI 1726 GTTAAC GTT^AAC
KspI 1741 CCGCGG CCGC^GG
MflI 2671 GGATCT [GA]^GATC[CT]
MluNI 2512 TGGCCA TGG^CCA
MroI 615 TCCGGA T^CCGGA
MscI 2512 TGGCCA TGG^CCA
NdeI 1820 CATATG CA^TATG
NspI 2441 GCATGC [GA]CATG^[CT]
PmaCI 1762 CACGTG CAC^GTG
PmlI 1762 CACGTG CAC^GTG
PvuII 2592 CAGCTG CAG^CTG
SacII 1741 CCGCGG CCGC^GG
ScaI 1941 AGTACT AGT^ACT
SgrAI 389 CGCCGGTG C[GA]^CCGG[CT]G
SphI 2441 GCATGC GCATG^C
StuI 2027 AGGCCT AGG^CCT
XcmI 1313 CCAGGGTATCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2671 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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Alw44I 1525 GTGCAC G^TGCAC
2455 GTGCAC
ApaLI 1525 GTGCAC G^TGCAC
2455 GTGCAC
BanII 2058 GAGCCC G[GA]GC[CT]^C
2283 GGGCCC
BbeI 2019 GGCGCC GGCGC^C
2050 GGCGCC
BbsI 863 GAAGAC GAAGAC(2/6)
2321 GTCTTC
BciVI 1318 GTATCC GTATCC(6/5)
2521 GTATCC
BpuAI 863 GAAGAC GAAGAC(2/6)
2321 GTCTTC
BsaAI 1516 TACGTG [CT]AC^GT[GA]
1762 CACGTG
BsaWI 615 TCCGGA [AT]^CCGG[AT]
2632 TCCGGT
BsgI 178 GTGCAG GTGCAG(16/14)
303 GTGCAG
BsmBI 102 CGTCTC CGTCTC(1/5)
806 GAGACG
BsmI 492 GAATGC GAATGC(1/-1)
2474 GCATTC
Bsp1407I 997 TGTACA T^GTACA
1874 TGTACA
BsrGI 997 TGTACA T^GTACA
1874 TGTACA
DraI 585 TTTAAA TTT^AAA
2602 TTTAAA
EarI 2031 CTCTTC CTCTTC(1/4)
487 GAAGAG
EcoRI 64 GAATTC G^AATTC
258 GAATTC
KasI 2019 GGCGCC G^GCGCC
2050 GGCGCC
Ksp632I 2031 CTCTTC CTCTTC(1/4)
487 GAAGAG
MluI 1808 ACGCGT A^CGCGT
1897 ACGCGT
NarI 2019 GGCGCC GG^CGCC
2050 GGCGCC
PpuMI 149 AGGACCT [GA]G^G[AT]CC[CT]
215 GGGACCT
SfoI 2019 GGCGCC GGC^GCC
2050 GGCGCC
SspBI 997 TGTACA T^GTACA
1874 TGTACA
TfiI 430 GAATC G^A[AT]TC
541 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaII BanI BbvI BclI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EcoNI
EcoO109I EcoRII FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I