Restriction map for
pFN21AB3329
(Linear) (Six-base) MAPSORT of: KIBB3329.seq Check: 1507 from: 1 to: 2847
KIBB3329.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1641 2847
Size: 1641 1206
AccI GT'mk_AC
Cuts at: 0 2149 2847
Size: 2149 698
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1061 2295 2847
Size: 1061 1234 552
Fragments arranged by size:
1234 1061 552
AflIII A'CryG_T
Cuts at: 0 1247 1517 1808 1897 2087 2847
Size: 1247 270 291 89 190 760
Fragments arranged by size:
1247 760 291 270 190 89
AleI CACnn'nnGTG
Cuts at: 0 130 2212 2847
Size: 130 2082 635
Fragments arranged by size:
2082 635 130
AlwNI CAG_nnn'CTG
Cuts at: 0 153 186 2068 2847
Size: 153 33 1882 779
Fragments arranged by size:
1882 779 153 33
ApaI G_GGCC'C
Cuts at: 0 2287 2847
Size: 2287 560
ApaLI G'TGCA_C
Cuts at: 0 1525 2455 2847
Size: 1525 930 392
Fragments arranged by size:
1525 930 392
ApoI r'AATT_y
Cuts at: 0 64 258 2656 2847
Size: 64 194 2398 191
Fragments arranged by size:
2398 194 191 64
AvaI C'yCGr_G
Cuts at: 0 1369 2847
Size: 1369 1478
BanI G'GyrC_C
Cuts at: 0 28 124 670 2019 2050 2847
Size: 28 96 546 1349 31 797
Fragments arranged by size:
1349 797 546 96 31 28
BanII G_rGCy'C
Cuts at: 0 2062 2287 2847
Size: 2062 225 560
Fragments arranged by size:
2062 560 225
BbeI G_GCGC'C
Cuts at: 0 2023 2054 2847
Size: 2023 31 793
Fragments arranged by size:
2023 793 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 870 2314 2847
Size: 870 1444 533
Fragments arranged by size:
1444 870 533
BciVI GTATCCnnnnn_n'
Cuts at: 0 1329 2532 2847
Size: 1329 1203 315
Fragments arranged by size:
1329 1203 315
BclI T'GATC_A
Cuts at: 0 1109 2096 2502 2847
Size: 1109 987 406 345
Fragments arranged by size:
1109 987 406 345
BlpI GC'TnA_GC
Cuts at: 0 1085 2847
Size: 1085 1762
Bme1580I G_kGCm'C
Cuts at: 0 31 1529 1905 2287 2459 2847
Size: 31 1498 376 382 172 388
Fragments arranged by size:
1498 388 382 376 172 31
BmgBI CAC'GTC
Cuts at: 0 1338 2847
Size: 1338 1509
BmrI ACTGGGnnnn_n'
Cuts at: 0 1063 2312 2847
Size: 1063 1249 535
Fragments arranged by size:
1249 1063 535
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1935 2847
Size: 1935 912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 244 337 1600 2549 2847
Size: 181 63 93 1263 949 298
Fragments arranged by size:
1263 949 298 181 93 63
BsaI GGTCTCn'nnnn_
Cuts at: 0 2752 2847
Size: 2752 95
BsaAI yAC'GTr
Cuts at: 0 1518 1764 2847
Size: 1518 246 1083
Fragments arranged by size:
1518 1083 246
BsaHI Gr'CG_yC
Cuts at: 0 199 1165 2020 2051 2847
Size: 199 966 855 31 796
Fragments arranged by size:
966 855 796 199 31
BsaWI w'CCGG_w
Cuts at: 0 615 2632 2847
Size: 615 2017 215
Fragments arranged by size:
2017 615 215
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1474 1504 1709 1739 2356 2386 2410 2440
Size: 1474 30 205 30 617 30 24 30
Cuts at: 2440 2847
Size: 407
Fragments arranged by size:
1474 617 407 205 30 30 30 30
24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 49 124 292 1588 2293 2359 2479
Size: 46 3 75 168 1296 705 66 120
Cuts at: 2479 2770 2847
Size: 291 77
Fragments arranged by size:
1296 705 291 168 120 77 75 66
46 3
BseYI C'CCAG_C
Cuts at: 0 1090 2847
Size: 1090 1757
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 324 2847
Size: 199 125 2523
Fragments arranged by size:
2523 199 125
BsiEI CG_ry'CG
Cuts at: 0 273 1500 1747 2847
Size: 273 1227 247 1100
Fragments arranged by size:
1227 1100 273 247
BsiHKAI G_wGCw'C
Cuts at: 0 785 1529 2221 2275 2459 2847
Size: 785 744 692 54 184 388
Fragments arranged by size:
785 744 692 388 184 54
BsmI GAATG_Cn'
Cuts at: 0 498 2474 2847
Size: 498 1976 373
Fragments arranged by size:
1976 498 373
BsmBI CGTCTCn'nnnn_
Cuts at: 0 108 800 2847
Size: 108 692 2047
Fragments arranged by size:
2047 692 108
Bsp1286I G_dGCh'C
Cuts at: 0 31 785 1529 1905 2062 2221 2275 2287
Size: 31 754 744 376 157 159 54 12
Cuts at: 2287 2459 2847
Size: 172 388
Fragments arranged by size:
754 744 388 376 172 159 157 54
31 12
BspEI T'CCGG_A
Cuts at: 0 615 2847
Size: 615 2232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 333 979 1162 1641 2847
Size: 333 646 183 479 1206
Fragments arranged by size:
1206 646 479 333 183
BsrBI CCG'CTC
Cuts at: 0 83 1485 1538 2012 2847
Size: 83 1402 53 474 835
Fragments arranged by size:
1402 835 474 83 53
BsrDI GCAATG_nn'
Cuts at: 0 1100 2847
Size: 1100 1747
BsrFI r'CCGG_y
Cuts at: 0 390 1683 1833 1965 2847
Size: 390 1293 150 132 882
Fragments arranged by size:
1293 882 390 150 132
BsrGI T'GTAC_A
Cuts at: 0 997 1874 2847
Size: 997 877 973
Fragments arranged by size:
997 973 877
BssHII G'CGCG_C
Cuts at: 0 1502 1604 1606 1779 2192 2847
Size: 1502 102 2 173 413 655
Fragments arranged by size:
1502 655 413 173 102 2
BssSI C'ACGA_G
Cuts at: 0 1570 2847
Size: 1570 1277
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1789 2847
Size: 1789 1058
BstEII G'GTnAC_C
Cuts at: 0 1149 2847
Size: 1149 1698
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1992 2112 2127 2847
Size: 1992 120 15 720
Fragments arranged by size:
1992 720 120 15
BstYI r'GATC_y
Cuts at: 0 2671 2847
Size: 2671 176
BstZ17I GTA'TAC
Cuts at: 0 2150 2847
Size: 2150 697
BtgI C'CryG_G
Cuts at: 0 1307 1322 1741 1979 2847
Size: 1307 15 419 238 868
Fragments arranged by size:
1307 868 419 238 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 126 1625 2085 2847
Size: 126 1499 460 762
Fragments arranged by size:
1499 762 460 126
BtsI GCAGTG_nn'
Cuts at: 0 1687 1790 2515 2847
Size: 1687 103 725 332
Fragments arranged by size:
1687 725 332 103
DraI TTT'AAA
Cuts at: 0 587 2604 2847
Size: 587 2017 243
Fragments arranged by size:
2017 587 243
DraIII CAC_nnn'GTG
Cuts at: 0 1767 2847
Size: 1767 1080
DrdI GACnn_nn'nnGTC
Cuts at: 0 814 1059 1135 2847
Size: 814 245 76 1712
Fragments arranged by size:
1712 814 245 76
EaeI y'GGCC_r
Cuts at: 0 84 1310 1331 1976 1982 2512 2847
Size: 84 1226 21 645 6 530 335
Fragments arranged by size:
1226 645 530 335 84 21 6
EarI CTCTTCn'nnn_
Cuts at: 0 482 2037 2847
Size: 482 1555 810
Fragments arranged by size:
1555 810 482
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 184 2847
Size: 184 2663
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 244 337 1061 1600 2295 2549 2847
Size: 181 63 93 724 539 695 254 298
Fragments arranged by size:
724 695 539 298 254 181 93 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 223 1909 2464 2847
Size: 223 1686 555 383
Fragments arranged by size:
1686 555 383 223
EcoO109I rG'GnC_Cy
Cuts at: 0 150 216 2283 2497 2847
Size: 150 66 2067 214 350
Fragments arranged by size:
2067 350 214 150 66
EcoRI G'AATT_C
Cuts at: 0 64 258 2847
Size: 64 194 2589
Fragments arranged by size:
2589 194 64
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 793 2847
Size: 793 2054
HaeII r_GCGC'y
Cuts at: 0 186 1232 1972 2023 2054 2158 2847
Size: 186 1046 740 51 31 104 689
Fragments arranged by size:
1046 740 689 186 104 51 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 61 1114 1146 1780 1812 2356 2388
Size: 29 32 1053 32 634 32 544 32
Cuts at: 2388 2847
Size: 459
Fragments arranged by size:
1053 634 544 459 32 32 32 32
29
HincII GTy'rAC
Cuts at: 0 705 1140 1728 2085 2583 2847
Size: 705 435 588 357 498 264
Fragments arranged by size:
705 588 498 435 357 264
HpaI GTT'AAC
Cuts at: 0 1728 2847
Size: 1728 1119
KasI G'GCGC_C
Cuts at: 0 2019 2050 2847
Size: 2019 31 797
Fragments arranged by size:
2019 797 31
MluI A'CGCG_T
Cuts at: 0 1808 1897 2847
Size: 1808 89 950
Fragments arranged by size:
1808 950 89
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1544 2847
Size: 1544 1303
MscI TGG'CCA
Cuts at: 0 2514 2847
Size: 2514 333
MslI CAynn'nnrTG
Cuts at: 0 130 2110 2212 2847
Size: 130 1980 102 635
Fragments arranged by size:
1980 635 130 102
MspA1I CmG'CkG
Cuts at: 0 339 1743 2594 2847
Size: 339 1404 851 253
Fragments arranged by size:
1404 851 339 253
NaeI GCC'GGC
Cuts at: 0 1685 1835 1967 2847
Size: 1685 150 132 880
Fragments arranged by size:
1685 880 150 132
NarI GG'CG_CC
Cuts at: 0 2020 2051 2847
Size: 2020 31 796
Fragments arranged by size:
2020 796 31
NdeI CA'TA_TG
Cuts at: 0 1821 2847
Size: 1821 1026
NgoMIV G'CCGG_C
Cuts at: 0 1683 1833 1965 2847
Size: 1683 150 132 882
Fragments arranged by size:
1683 882 150 132
NspI r_CATG'y
Cuts at: 0 2445 2847
Size: 2445 402
PflMI CCAn_nnn'nTGG
Cuts at: 0 553 696 1767 2847
Size: 553 143 1071 1080
Fragments arranged by size:
1080 1071 553 143
PfoI T'CCnGG_A
Cuts at: 0 2035 2490 2847
Size: 2035 455 357
Fragments arranged by size:
2035 455 357
PmlI CAC'GTG
Cuts at: 0 1764 2847
Size: 1764 1083
PpuMI rG'GwC_Cy
Cuts at: 0 150 216 2847
Size: 150 66 2631
Fragments arranged by size:
2631 150 66
PsiI TTA'TAA
Cuts at: 0 944 2847
Size: 944 1903
PspOMI G'GGCC_C
Cuts at: 0 2283 2847
Size: 2283 564
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1366 1398 2847
Size: 1366 32 1449
Fragments arranged by size:
1449 1366 32
PstI C_TGCA'G
Cuts at: 0 239 344 1559 1652 2847
Size: 239 105 1215 93 1195
Fragments arranged by size:
1215 1195 239 105 93
PvuII CAG'CTG
Cuts at: 0 2594 2847
Size: 2594 253
SacII CC_GC'GG
Cuts at: 0 1744 2847
Size: 1744 1103
ScaI AGT'ACT
Cuts at: 0 1943 2847
Size: 1943 904
SfcI C'TryA_G
Cuts at: 0 40 235 340 507 641 962 1555 1588
Size: 40 195 105 167 134 321 593 33
Cuts at: 1588 1648 2847
Size: 60 1199
Fragments arranged by size:
1199 593 321 195 167 134 105 60
40 33
SfoI GGC'GCC
Cuts at: 0 2021 2052 2847
Size: 2021 31 795
Fragments arranged by size:
2021 795 31
SgrAI Cr'CCGG_yG
Cuts at: 0 390 2847
Size: 390 2457
SphI G_CATG'C
Cuts at: 0 2445 2847
Size: 2445 402
StuI AGG'CCT
Cuts at: 0 2029 2847
Size: 2029 818
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 818 996 1741 2847
Size: 818 178 745 1106
Fragments arranged by size:
1106 818 745 178
TatI w'GTAC_w
Cuts at: 0 954 997 1373 1874 1941 2847
Size: 954 43 376 501 67 906
Fragments arranged by size:
954 906 501 376 67 43
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1320 2847
Size: 1320 1527
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BciVI BclI
BlpI Bme1580I BmgBI BmrI BplI BpmI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII HpaI KasI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PflMI PfoI PmlI
PpuMI PsiI PspOMI PsrI PstI PvuII SacII ScaI
SfcI SfoI SgrAI SphI StuI TaqII TatI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BfrBI BglI BglII BmtI Bpu10I BpuEI BsaBI
BsiWI BspHI BstBI Bsu36I ClaI CspCI EagI EcoICRI
EcoRV FseI FspI FspAI HindIII KpnI MfeI NcoI
NheI NotI NruI NsiI PacI PasI PciI PmeI
PpiI PshAI PspXI PvuI RsrII SacI SalI SanDI
SapI SbfI SexAI SfiI SgfI SmaI SmlI SnaBI
SpeI SrfI SspI StyI SwaI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI