(Circular) (Six-base) MAPSORT of: pFN21AB3327.seq Check: 462 from: 1 to: 7390 pFN21AB3327 7390 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2838 3704 2838 Size: 866 6524 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5200 278 Size: 53 83 186 936 3664 2468 Fragments arranged by size: 3664 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4616 4733 1937 Size: 2679 117 4594 Fragments arranged by size: 4594 2679 117 Acc65I G'GTAC_C Cuts at: 2042 4711 2042 Size: 2669 4721 AclI AA'CG_TT Cuts at: 1563 5517 5890 1563 Size: 3954 373 3063 Fragments arranged by size: 3954 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2493 4578 5453 6837 7105 1632 Size: 861 2085 875 1384 268 1917 Fragments arranged by size: 2085 1917 1384 875 861 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7193 AflIII A'CryG_T Cuts at: 1203 2839 3101 6289 7266 1203 Size: 1636 262 3188 977 1327 Fragments arranged by size: 3188 1636 1327 977 262 AgeI A'CCGG_T Cuts at: 3263 6221 3263 Size: 2958 4432 AhdI GACnn_n'nnGTC Cuts at: 3546 3683 6119 3546 Size: 137 2436 4817 Fragments arranged by size: 4817 2436 137 AleI CACnn'nnGTG Cuts at: 1166 2580 2802 1166 Size: 1414 222 5754 Fragments arranged by size: 5754 1414 222 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4553 1184 Size: 3369 4021 AlwNI CAG_nnn'CTG Cuts at: 3143 4297 5133 6705 3143 Size: 1154 836 1572 3828 Fragments arranged by size: 3828 1572 1154 836 ApaLI G'TGCA_C Cuts at: 5447 6603 5447 Size: 1156 6234 ApoI r'AATT_y Cuts at: 1493 2931 3285 3807 3949 4699 4925 5077 7186 Size: 1438 354 522 142 750 226 152 2109 Cuts at: 7186 1493 Size: 1697 Fragments arranged by size: 2109 1697 1438 750 522 354 226 152 142 AseI AT'TA_AT Cuts at: 160 1032 5944 160 Size: 872 4912 1606 Fragments arranged by size: 4912 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7390 AvaI C'yCGr_G Cuts at: 1870 1943 4715 1870 Size: 73 2772 4545 Fragments arranged by size: 4545 2772 73 AvrII C'CTAG_G Cuts at: 2491 2491 Size: 7390 BamHI G'GATC_C Cuts at: 2404 3874 4720 5087 2404 Size: 1470 846 367 4707 Fragments arranged by size: 4707 1470 846 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 2756 3388 3739 3962 Size: 325 217 627 255 714 632 351 223 Cuts at: 3962 4537 4711 6166 618 Size: 575 174 1455 1842 Fragments arranged by size: 1842 1455 714 632 627 575 351 325 255 223 217 174 BanII G_rGCy'C Cuts at: 1391 2274 3959 4709 1391 Size: 883 1685 750 4072 Fragments arranged by size: 4072 1685 883 750 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2165 3266 3330 3542 4083 4392 928 Size: 971 266 1101 64 212 541 309 3926 Fragments arranged by size: 3926 1101 971 541 309 266 212 64 BbvCI CC'TCA_GC Cuts at: 3763 3763 Size: 7390 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5581 5615 1903 Size: 34 3644 34 3678 Fragments arranged by size: 3678 3644 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5282 6492 5282 Size: 1210 6180 BclI T'GATC_A Cuts at: 1548 2794 2962 1548 Size: 1246 168 5976 Fragments arranged by size: 5976 1246 168 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2496 6001 136 Size: 107 122 71 631 605 824 3505 1525 Fragments arranged by size: 3505 1525 824 631 605 122 107 71 BglII A'GATC_T Cuts at: 7385 7385 Size: 7390 BlpI GC'TnA_GC Cuts at: 2268 4807 2268 Size: 2539 4851 Bme1580I G_kGCm'C Cuts at: 1790 5451 6607 1790 Size: 3661 1156 2573 Fragments arranged by size: 3661 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7390 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5121 6079 442 Size: 416 53 83 399 24 3704 958 1753 Fragments arranged by size: 3704 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4440 1056 Size: 3384 4006 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2469 2487 6050 1239 Size: 1230 18 3563 2579 Fragments arranged by size: 3563 2579 1230 18 Bpu10I CC'TnA_GC Cuts at: 3763 3763 Size: 7390 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5515 6380 6678 5515 Size: 865 298 6227 Fragments arranged by size: 6227 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2487 3533 3691 6053 882 Size: 617 988 1046 158 2362 2219 Fragments arranged by size: 2362 2219 1046 988 617 158 BsaAI yAC'GTr Cuts at: 493 1204 5181 493 Size: 711 3977 2702 Fragments arranged by size: 3977 2702 711 BsaBI GATnn'nnATC Cuts at: 7384 7384 Size: 7390 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2321 2520 5197 5579 Size: 53 83 186 936 788 199 2677 382 Cuts at: 5579 6214 275 Size: 635 1451 Fragments arranged by size: 2677 1451 936 788 635 382 199 186 83 53 BsaWI w'CCGG_w Cuts at: 3263 5823 6221 6495 6642 7053 3263 Size: 2560 398 274 147 411 3600 Fragments arranged by size: 3600 2560 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2405 2435 3671 3701 4129 Size: 30 165 30 1002 30 1236 30 428 Cuts at: 4129 4159 1178 Size: 30 4409 Fragments arranged by size: 4409 1236 1002 428 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2233 2426 2648 3751 1186 Size: 1047 193 222 1103 4825 Fragments arranged by size: 4825 1103 1047 222 193 BseYI C'CCAG_C Cuts at: 2571 6593 7089 2571 Size: 4022 496 2872 Fragments arranged by size: 4022 2872 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2896 2989 3156 1163 Size: 573 1160 93 167 5397 Fragments arranged by size: 5397 1160 573 167 93 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2201 2214 2265 4840 5601 6629 Size: 275 186 200 13 51 2575 761 1028 Cuts at: 6629 1540 Size: 2301 Fragments arranged by size: 2575 2301 1028 761 275 200 186 51 13 BsiHKAI G_wGCw'C Cuts at: 5451 5536 6607 5451 Size: 85 1071 6234 Fragments arranged by size: 6234 1071 85 BsmI GAATG_Cn' Cuts at: 4901 4994 4901 Size: 93 7297 BsmBI CGTCTCn'nnnn_ Cuts at: 4149 4149 Size: 7390 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2274 3959 4709 5451 5536 6607 1391 Size: 399 484 1685 750 742 85 1071 2174 Fragments arranged by size: 2174 1685 1071 750 742 484 399 85 BspEI T'CCGG_A Cuts at: 7053 7053 Size: 7390 BspHI T'CATG_A Cuts at: 3793 5279 7009 3793 Size: 1486 1730 4174 Fragments arranged by size: 4174 1730 1486 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2838 3704 4745 844 Size: 1994 866 1041 3489 Fragments arranged by size: 3489 1994 1041 866 BsrBI CCG'CTC Cuts at: 1394 1823 5277 1394 Size: 429 3454 3507 Fragments arranged by size: 3507 3454 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2234 4196 5885 6059 65 Size: 1183 986 1962 1689 174 1396 Fragments arranged by size: 1962 1689 1396 1183 986 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2045 2200 2314 3263 6034 6221 1700 Size: 110 235 155 114 949 2771 187 2869 Fragments arranged by size: 2869 2771 949 235 187 155 114 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7390 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7390 BssSI C'ACGA_G Cuts at: 3897 5450 6462 3897 Size: 1553 1012 4825 Fragments arranged by size: 4825 1553 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7390 BstBI TT'CG_AA Cuts at: 6194 6194 Size: 7390 BstEII G'GTnAC_C Cuts at: 1184 2182 1184 Size: 998 6392 BstXI CCAn_nnnn'nTGG Cuts at: 2450 2450 Size: 7390 BstYI r'GATC_y Cuts at: 1972 2404 3874 4229 4447 4720 5087 5472 5489 Size: 432 1470 355 218 273 367 385 17 Cuts at: 5489 6941 7027 7039 7385 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1470 1452 432 385 367 355 346 273 218 86 17 12 BstZ17I GTA'TAC Cuts at: 4617 4617 Size: 7390 Bsu36I CC'TnA_GG Cuts at: 2409 3916 2409 Size: 1507 5883 BtgI C'CryG_G Cuts at: 513 1065 2328 2400 2623 513 Size: 552 1263 72 223 5280 Fragments arranged by size: 5280 1263 552 223 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6651 Fragments arranged by size: 6651 683 56 BtsI GCAGTG_nn' Cuts at: 2023 4906 5700 5720 2023 Size: 2883 794 20 3693 Fragments arranged by size: 3693 2883 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7390 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7355 DraI TTT'AAA Cuts at: 4694 4694 Size: 7390 DraIII CAC_nnn'GTG Cuts at: 3023 3023 Size: 7390 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2654 4597 6397 809 Size: 507 1338 1943 1800 1802 Fragments arranged by size: 1943 1802 1800 1338 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2198 2211 2262 Size: 54 1425 50 275 18 368 13 51 Cuts at: 2262 4165 4837 5726 7355 8 Size: 1903 672 889 1629 43 Fragments arranged by size: 1903 1629 1425 889 672 368 275 54 51 50 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 2198 2211 2262 4837 1537 Size: 275 386 13 51 2575 4090 Fragments arranged by size: 4090 2575 386 275 51 13 EarI CTCTTCn'nnn_ Cuts at: 1353 2469 3377 5320 1353 Size: 1116 908 1943 3423 Fragments arranged by size: 3423 1943 1116 908 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2052 2271 5977 6351 6497 387 Size: 1064 138 463 219 3706 374 146 1280 Fragments arranged by size: 3706 1280 1064 463 374 219 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2469 2487 2493 4578 5453 6050 6837 Size: 393 837 18 6 2085 875 597 787 Cuts at: 6837 7105 1239 Size: 268 1524 Fragments arranged by size: 2085 1524 875 837 787 597 393 268 18 6 EcoNI CCTnn'n_nnAGG Cuts at: 2344 4022 2344 Size: 1678 5712 EcoO109I rG'GnC_Cy Cuts at: 2349 2530 2349 Size: 181 7209 EcoRI G'AATT_C Cuts at: 3807 4699 5077 3807 Size: 892 378 6120 Fragments arranged by size: 6120 892 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2739 2739 Size: 7390 FspI TGC'GCA Cuts at: 5896 5896 Size: 7390 HaeII r_GCGC'y Cuts at: 1678 6537 1678 Size: 4859 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2405 2437 2829 2861 3532 Size: 32 135 32 833 32 392 32 671 Cuts at: 3532 3564 3669 3701 3830 3862 4300 4322 4332 Size: 32 105 32 129 32 438 22 10 Cuts at: 4332 4354 4408 4435 4440 4467 6031 6063 6105 Size: 22 54 27 5 27 1564 32 42 Cuts at: 6105 6137 1373 Size: 32 2626 Fragments arranged by size: 2626 1564 833 671 438 392 135 129 105 54 42 32 32 32 32 32 32 32 32 32 27 27 22 22 10 5 HincII GTy'rAC Cuts at: 669 1657 1938 4281 4734 4980 669 Size: 988 281 2343 453 246 3079 Fragments arranged by size: 3079 2343 988 453 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7390 HpaI GTT'AAC Cuts at: 4980 4980 Size: 7390 KpnI G_GTAC'C Cuts at: 2046 4715 2046 Size: 2669 4721 MfeI C'AATT_G Cuts at: 4989 4989 Size: 7390 MluI A'CGCG_T Cuts at: 7266 7266 Size: 7390 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2722 3873 4045 6504 6688 1305 Size: 1417 1151 172 2459 184 2007 Fragments arranged by size: 2459 2007 1417 1151 184 172 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5568 Fragments arranged by size: 5568 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2448 2550 2580 2790 2802 2956 3040 Size: 648 1282 102 30 210 12 154 84 Cuts at: 3040 5348 5707 5866 518 Size: 2308 359 159 2042 Fragments arranged by size: 2308 2042 1282 648 359 210 159 154 102 84 30 12 MspA1I CmG'CkG Cuts at: 1606 2112 2267 2330 2402 4806 5483 6631 6876 Size: 506 155 63 72 2404 677 1148 245 Cuts at: 6876 1606 Size: 2120 Fragments arranged by size: 2404 2120 1148 677 506 245 155 72 63 NaeI GCC'GGC Cuts at: 2202 2316 2202 Size: 114 7276 NcoI C'CATG_G Cuts at: 513 1065 2623 513 Size: 552 1558 5280 Fragments arranged by size: 5280 1558 552 NdeI CA'TA_TG Cuts at: 387 2997 387 Size: 2610 4780 NgoMIV G'CCGG_C Cuts at: 2200 2314 2200 Size: 114 7276 NheI G'CTAG_C Cuts at: 1052 4436 1052 Size: 3384 4006 NotI GC'GGCC_GC Cuts at: 4837 4837 Size: 7390 NspI r_CATG'y Cuts at: 1132 1229 2555 2843 3105 3307 4748 6293 1132 Size: 97 1326 288 262 202 1441 1545 2229 Fragments arranged by size: 2229 1545 1441 1326 288 262 202 97 PasI CC'CwG_GG Cuts at: 1348 2146 1348 Size: 798 6592 PciI A'CATG_T Cuts at: 2839 3101 6289 2839 Size: 262 3188 3940 Fragments arranged by size: 3940 3188 262 PflMI CCAn_nnn'nTGG Cuts at: 1108 2152 4297 1108 Size: 1044 2145 4201 Fragments arranged by size: 4201 2145 1044 PfoI T'CCnGG_A Cuts at: 2526 2526 Size: 7390 PmeI GTTT'AAAC Cuts at: 4694 4694 Size: 7390 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5459 5491 6972 7004 5459 Size: 32 1481 32 5845 Fragments arranged by size: 5845 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2349 2530 2349 Size: 181 7209 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7390 PsiI TTA'TAA Cuts at: 4960 4960 Size: 7390 PstI C_TGCA'G Cuts at: 830 3381 3715 4742 830 Size: 2551 334 1027 3478 Fragments arranged by size: 3478 2551 1027 334 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7390 SacII CC_GC'GG Cuts at: 2331 2403 2331 Size: 72 7318 SalI G'TCGA_C Cuts at: 1936 4732 1936 Size: 2796 4594 SbfI CC_TGCA'GG Cuts at: 4742 4742 Size: 7390 ScaI AGT'ACT Cuts at: 5638 7350 5638 Size: 1712 5678 SfcI C'TryA_G Cuts at: 826 1045 2800 2915 3377 3711 4738 5873 6554 Size: 219 1755 115 462 334 1027 1135 681 Cuts at: 6554 6745 826 Size: 191 1471 Fragments arranged by size: 1755 1471 1135 1027 681 462 334 219 191 115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7390 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7390 SmaI CCC'GGG Cuts at: 1872 4717 1872 Size: 2845 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5494 6395 6657 820 Size: 197 926 3551 901 262 1553 Fragments arranged by size: 3551 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5181 493 Size: 4688 2702 SpeI A'CTAG_T Cuts at: 152 3131 152 Size: 2979 4411 SphI G_CATG'C Cuts at: 1132 4748 1132 Size: 3616 3774 SspI AAT'ATT Cuts at: 5 52 3886 5314 5 Size: 47 3834 1428 2081 Fragments arranged by size: 3834 2081 1428 47 StuI AGG'CCT Cuts at: 1517 2490 1517 Size: 973 6417 StyI C'CwwG_G Cuts at: 513 1065 2491 2623 4829 513 Size: 552 1426 132 2206 3074 Fragments arranged by size: 3074 2206 1426 552 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3980 4684 5417 5434 5587 5772 6217 Size: 1255 2400 704 733 17 153 185 445 Cuts at: 6217 325 Size: 1498 Fragments arranged by size: 2400 1498 1255 733 704 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3560 4652 5636 Size: 274 80 33 51 1443 1583 1092 984 Cuts at: 5636 7348 96 Size: 1712 138 Fragments arranged by size: 1712 1583 1443 1092 984 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2701 2733 3007 3039 3671 3703 4102 4129 4134 Size: 32 274 32 632 32 399 27 5 Cuts at: 4134 4161 7350 7382 2701 Size: 27 3189 32 2709 Fragments arranged by size: 3189 2709 632 399 274 32 32 32 32 27 27 5 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7390 XbaI T'CTAG_A Cuts at: 4726 4726 Size: 7390 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2447 2734 3563 2447 Size: 287 829 6274 Fragments arranged by size: 6274 829 287 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7390 XmaI C'CCGG_G Cuts at: 1870 4715 1870 Size: 2845 4545 XmnI GAAnn'nnTTC Cuts at: 3936 5519 3936 Size: 1583 5807 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5198 276 Size: 53 83 186 936 3664 2468 Fragments arranged by size: 3664 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI