(Circular) (Six-base) MAPSORT of: pFN21AB3321.seq Check: 6650 from: 1 to: 7474 pFN21AB3321 7474 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2029 2345 3790 3815 2029 Size: 316 1445 25 5688 Fragments arranged by size: 5688 1445 316 25 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5284 278 Size: 53 83 186 936 3748 2468 Fragments arranged by size: 3748 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3161 4401 4817 1937 Size: 1224 1240 416 4594 Fragments arranged by size: 4594 1240 1224 416 Acc65I G'GTAC_C Cuts at: 4795 4795 Size: 7474 AclI AA'CG_TT Cuts at: 1563 5601 5974 1563 Size: 4038 373 3063 Fragments arranged by size: 4038 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4739 5537 6921 7189 1632 Size: 3107 798 1384 268 1917 Fragments arranged by size: 3107 1917 1384 798 268 AfeI AGC'GCT Cuts at: 4121 4121 Size: 7474 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7277 AflIII A'CryG_T Cuts at: 1203 6373 7350 1203 Size: 5170 977 1327 Fragments arranged by size: 5170 1327 977 AgeI A'CCGG_T Cuts at: 2550 3156 6305 2550 Size: 606 3149 3719 Fragments arranged by size: 3719 3149 606 AhdI GACnn_n'nnGTC Cuts at: 2438 6203 2438 Size: 3765 3709 AleI CACnn'nnGTG Cuts at: 1166 4499 1166 Size: 3333 4141 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3107 3867 1184 Size: 1923 760 4791 Fragments arranged by size: 4791 1923 760 AlwNI CAG_nnn'CTG Cuts at: 2179 2215 2479 3657 3805 4090 4672 4698 4717 Size: 36 264 1178 148 285 582 26 19 Cuts at: 4717 5217 6789 2179 Size: 500 1572 2864 Fragments arranged by size: 2864 1572 1178 582 500 285 264 148 36 26 19 ApaI G_GGCC'C Cuts at: 2565 3954 4082 4201 2565 Size: 1389 128 119 5838 Fragments arranged by size: 5838 1389 128 119 ApaLI G'TGCA_C Cuts at: 5531 6687 5531 Size: 1156 6318 ApoI r'AATT_y Cuts at: 1493 4783 5009 5161 7270 1493 Size: 3290 226 152 2109 1697 Fragments arranged by size: 3290 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6028 160 Size: 872 4996 1606 Fragments arranged by size: 4996 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7474 AvaI C'yCGr_G Cuts at: 1870 1943 2558 2564 2570 2964 4194 4364 4758 Size: 73 615 6 6 394 1230 170 394 Cuts at: 4758 4799 1870 Size: 41 4545 Fragments arranged by size: 4545 1230 615 394 394 170 73 41 6 6 BamHI G'GATC_C Cuts at: 4378 4804 5171 4378 Size: 426 367 6681 Fragments arranged by size: 6681 426 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2107 2492 3119 3176 4795 Size: 325 217 627 320 385 627 57 1619 Cuts at: 4795 6250 618 Size: 1455 1842 Fragments arranged by size: 1842 1619 1455 627 627 385 325 320 217 57 BanII G_rGCy'C Cuts at: 1391 2095 2565 2571 2577 3642 3882 3954 3965 Size: 704 470 6 6 1065 240 72 11 Cuts at: 3965 4082 4201 4793 1391 Size: 117 119 592 4072 Fragments arranged by size: 4072 1065 704 592 470 240 119 117 72 11 6 6 BbeI G_GCGC'C Cuts at: 3123 3123 Size: 7474 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2997 3277 928 Size: 971 1098 280 5125 Fragments arranged by size: 5125 1098 971 280 BbvCI CC'TCA_GC Cuts at: 3257 3524 3969 4286 3257 Size: 267 445 317 6445 Fragments arranged by size: 6445 445 317 267 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2300 2334 5665 5699 1903 Size: 34 363 34 3331 34 3678 Fragments arranged by size: 3678 3331 363 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4055 5366 6576 4055 Size: 1311 1210 4953 Fragments arranged by size: 4953 1311 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7474 BfrBI ATG'CAT Cuts at: 3584 3584 Size: 7474 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3544 3619 6085 Size: 107 122 71 631 605 1872 75 2466 Cuts at: 6085 136 Size: 1525 Fragments arranged by size: 2466 1872 1525 631 605 122 107 75 71 BglII A'GATC_T Cuts at: 3396 7469 3396 Size: 4073 3401 BlpI GC'TnA_GC Cuts at: 4891 4891 Size: 7474 Bme1580I G_kGCm'C Cuts at: 1790 2497 2565 2846 3954 4082 4188 4201 5535 Size: 707 68 281 1108 128 106 13 1334 Cuts at: 5535 6691 1790 Size: 1156 2573 Fragments arranged by size: 2573 1334 1156 1108 707 281 128 106 68 13 BmgBI CAC'GTC Cuts at: 1318 2104 1318 Size: 786 6688 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2527 4162 5205 Size: 416 53 83 399 24 1110 1635 1043 Cuts at: 5205 6163 442 Size: 958 1753 Fragments arranged by size: 1753 1635 1110 1043 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7474 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2296 3127 3129 2296 Size: 831 2 6641 Fragments arranged by size: 6641 831 2 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2204 2291 3122 3458 4048 4136 4787 6134 Size: 965 87 831 336 590 88 651 1347 Cuts at: 6134 1239 Size: 2579 Fragments arranged by size: 2579 1347 965 831 651 590 336 88 87 Bpu10I CC'TnA_GC Cuts at: 2297 3257 3524 3969 4208 4286 4673 2297 Size: 960 267 445 239 78 387 5098 Fragments arranged by size: 5098 960 445 387 267 239 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4223 5599 6464 6762 4223 Size: 1376 865 298 4935 Fragments arranged by size: 4935 1376 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2349 6137 882 Size: 617 850 3788 2219 Fragments arranged by size: 3788 2219 850 617 BsaAI yAC'GTr Cuts at: 493 1204 5265 493 Size: 711 4061 2702 Fragments arranged by size: 4061 2702 711 BsaBI GATnn'nnATC Cuts at: 7468 7468 Size: 7474 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2646 3120 5281 5663 Size: 53 83 186 936 1113 474 2161 382 Cuts at: 5663 6298 275 Size: 635 1451 Fragments arranged by size: 2161 1451 1113 936 635 474 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2358 2550 3156 4344 5907 6305 6579 6726 7137 Size: 192 606 1188 1563 398 274 147 411 Cuts at: 7137 2358 Size: 2695 Fragments arranged by size: 2695 1563 1188 606 411 398 274 192 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2588 2618 1178 Size: 30 165 30 1185 30 6034 Fragments arranged by size: 6034 1185 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2258 2612 2615 2732 3579 1186 Size: 1072 354 3 117 847 5081 Fragments arranged by size: 5081 1072 847 354 117 3 BseYI C'CCAG_C Cuts at: 2065 2303 2631 3293 3946 4075 4083 4200 6677 Size: 238 328 662 653 129 8 117 2477 Cuts at: 6677 7173 2065 Size: 496 2366 Fragments arranged by size: 2477 2366 662 653 496 328 238 129 117 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2711 3305 4544 1163 Size: 573 975 594 1239 4093 Fragments arranged by size: 4093 1239 975 594 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4924 5685 6713 1540 Size: 275 186 2923 761 1028 2301 Fragments arranged by size: 2923 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2080 2190 2298 3235 4679 5535 5620 6691 2080 Size: 110 108 937 1444 856 85 1071 2863 Fragments arranged by size: 2863 1444 1071 937 856 110 108 85 BsmI GAATG_Cn' Cuts at: 3586 4985 5078 3586 Size: 1399 93 5982 Fragments arranged by size: 5982 1399 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3896 3896 Size: 7474 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2080 2095 2190 2298 2497 2565 2571 Size: 399 290 15 95 108 199 68 6 Cuts at: 2571 2577 2846 3235 3642 3882 3954 3965 4082 Size: 6 269 389 407 240 72 11 117 Cuts at: 4082 4188 4201 4679 4793 5535 5620 6691 1391 Size: 106 13 478 114 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 478 407 399 389 290 269 240 199 117 114 108 106 95 85 72 68 15 13 11 6 6 BspEI T'CCGG_A Cuts at: 4344 7137 4344 Size: 2793 4681 BspHI T'CATG_A Cuts at: 2587 3235 4480 5363 7093 2587 Size: 648 1245 883 1730 2968 Fragments arranged by size: 2968 1730 1245 883 648 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2029 2345 3528 3790 3815 4829 844 Size: 1185 316 1183 262 25 1014 3489 Fragments arranged by size: 3489 1185 1183 1014 316 262 25 BsrBI CCG'CTC Cuts at: 1394 1823 2290 4327 5361 1394 Size: 429 467 2037 1034 3507 Fragments arranged by size: 3507 2037 1034 467 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5969 6143 65 Size: 1183 4721 174 1396 Fragments arranged by size: 4721 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2151 2550 3122 3156 3699 6118 6305 Size: 110 341 399 572 34 543 2419 187 Cuts at: 6305 1700 Size: 2869 Fragments arranged by size: 2869 2419 572 543 399 341 187 110 34 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7474 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7474 BssSI C'ACGA_G Cuts at: 4625 5534 6546 4625 Size: 909 1012 5553 Fragments arranged by size: 5553 1012 909 BstAPI GCAn_nnn'nTGC Cuts at: 794 3400 3830 794 Size: 2606 430 4438 Fragments arranged by size: 4438 2606 430 BstBI TT'CG_AA Cuts at: 2668 6278 2668 Size: 3610 3864 BstEII G'GTnAC_C Cuts at: 1184 2884 2905 1184 Size: 1700 21 5753 Fragments arranged by size: 5753 1700 21 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3070 3782 3874 4000 2011 Size: 1059 712 92 126 5485 Fragments arranged by size: 5485 1059 712 126 92 BstYI r'GATC_y Cuts at: 1972 2418 2870 3189 3396 4378 4804 5171 5556 Size: 446 452 319 207 982 426 367 385 Cuts at: 5556 5573 7025 7111 7123 7469 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 982 452 446 426 385 367 346 319 207 86 17 12 BstZ17I GTA'TAC Cuts at: 3162 4402 3162 Size: 1240 6234 Bsu36I CC'TnA_GG Cuts at: 2372 2372 Size: 7474 BtgI C'CryG_G Cuts at: 513 1065 2081 2577 2890 3213 3856 513 Size: 552 1016 496 313 323 643 4131 Fragments arranged by size: 4131 1016 643 552 496 323 313 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3565 487 Size: 683 56 2339 4396 Fragments arranged by size: 4396 2339 683 56 BtsI GCAGTG_nn' Cuts at: 2393 3539 4564 4990 5784 5804 2393 Size: 1146 1025 426 794 20 4063 Fragments arranged by size: 4063 1146 1025 794 426 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7474 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7439 DraI TTT'AAA Cuts at: 4778 4778 Size: 7474 DraIII CAC_nnn'GTG Cuts at: 2850 3715 4054 4072 2850 Size: 865 339 18 6252 Fragments arranged by size: 6252 865 339 18 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6481 809 Size: 507 5165 1802 Fragments arranged by size: 5165 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2018 2331 2398 Size: 54 1425 50 275 18 188 313 67 Cuts at: 2398 2607 3459 3536 3701 3864 4158 4296 4539 Size: 209 852 77 165 163 294 138 243 Cuts at: 4539 4921 5810 7439 8 Size: 382 889 1629 43 Fragments arranged by size: 1629 1425 889 852 382 313 294 275 243 209 188 165 163 138 77 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4921 1537 Size: 275 3109 4090 Fragments arranged by size: 4090 3109 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2691 4040 5404 1353 Size: 1338 1349 1364 3423 Fragments arranged by size: 3423 1364 1349 1338 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4348 6061 6435 6581 387 Size: 1064 138 2759 1713 374 146 1280 Fragments arranged by size: 2759 1713 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2291 3122 3458 4048 4136 4739 Size: 393 572 87 831 336 590 88 603 Cuts at: 4739 4787 5537 6134 6921 7189 1239 Size: 48 750 597 787 268 1524 Fragments arranged by size: 1524 831 787 750 603 597 590 572 393 336 268 88 87 48 EcoNI CCTnn'n_nnAGG Cuts at: 2273 2918 4625 2273 Size: 645 1707 5122 Fragments arranged by size: 5122 1707 645 EcoO109I rG'GnC_Cy Cuts at: 2639 3950 3997 4079 2639 Size: 1311 47 82 6034 Fragments arranged by size: 6034 1311 82 47 EcoRI G'AATT_C Cuts at: 4783 5161 4783 Size: 378 7096 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3043 3043 Size: 7474 FspI TGC'GCA Cuts at: 5980 5980 Size: 7474 HaeII r_GCGC'y Cuts at: 1678 3123 3300 4123 6621 1678 Size: 1445 177 823 2498 2531 Fragments arranged by size: 2531 2498 1445 823 177 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2424 2456 4398 4430 6115 Size: 32 135 32 852 32 1942 32 1685 Cuts at: 6115 6147 6189 6221 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1942 1685 852 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2878 3720 3904 4818 5064 669 Size: 988 281 940 842 184 914 246 3079 Fragments arranged by size: 3079 988 940 914 842 281 246 184 HindIII A'AGCT_T Cuts at: 748 748 Size: 7474 HpaI GTT'AAC Cuts at: 5064 5064 Size: 7474 KasI G'GCGC_C Cuts at: 3119 3119 Size: 7474 KpnI G_GTAC'C Cuts at: 4799 4799 Size: 7474 MfeI C'AATT_G Cuts at: 5073 5073 Size: 7474 MluI A'CGCG_T Cuts at: 7350 7350 Size: 7474 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2778 3186 3543 6588 6772 1305 Size: 1473 408 357 3045 184 2007 Fragments arranged by size: 3045 2007 1473 408 357 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2020 2333 3461 3538 3866 Size: 54 1425 343 188 313 1128 77 328 Cuts at: 3866 4160 4541 10 Size: 294 381 2943 Fragments arranged by size: 2943 1425 1128 381 343 328 313 294 188 77 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3330 4499 5432 5791 5950 518 Size: 648 843 1321 1169 933 359 159 2042 Fragments arranged by size: 2042 1321 1169 933 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2083 2176 2314 2635 2941 3657 3730 3946 Size: 477 93 138 321 306 716 73 216 Cuts at: 3946 4012 4204 4717 4738 4890 5567 6715 6960 Size: 66 192 513 21 152 677 1148 245 Cuts at: 6960 1606 Size: 2120 Fragments arranged by size: 2120 1148 716 677 513 477 321 306 245 216 192 152 138 93 73 66 21 NaeI GCC'GGC Cuts at: 3124 3701 3124 Size: 577 6897 NarI GG'CG_CC Cuts at: 3120 3120 Size: 7474 NcoI C'CATG_G Cuts at: 513 1065 2890 3213 513 Size: 552 1825 323 4774 Fragments arranged by size: 4774 1825 552 323 NdeI CA'TA_TG Cuts at: 387 387 Size: 7474 NgoMIV G'CCGG_C Cuts at: 3122 3699 3122 Size: 577 6897 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7474 NotI GC'GGCC_GC Cuts at: 4921 4921 Size: 7474 NsiI A_TGCA'T Cuts at: 3586 3586 Size: 7474 NspI r_CATG'y Cuts at: 1132 1229 4659 4832 6377 1132 Size: 97 3430 173 1545 2229 Fragments arranged by size: 3430 2229 1545 173 97 PasI CC'CwG_GG Cuts at: 1348 2428 1348 Size: 1080 6394 PciI A'CATG_T Cuts at: 6373 6373 Size: 7474 PflMI CCAn_nnn'nTGG Cuts at: 1108 2461 3421 3562 4054 4698 1108 Size: 1353 960 141 492 644 3884 Fragments arranged by size: 3884 1353 960 644 492 141 PfoI T'CCnGG_A Cuts at: 2447 2526 2447 Size: 79 7395 PmeI GTTT'AAAC Cuts at: 4778 4778 Size: 7474 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5543 5575 7056 7088 5543 Size: 32 1481 32 5929 Fragments arranged by size: 5929 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3997 3997 Size: 7474 PshAI GACnn'nnGTC Cuts at: 1613 2997 1613 Size: 1384 6090 PsiI TTA'TAA Cuts at: 5044 5044 Size: 7474 PspOMI G'GGCC_C Cuts at: 2561 3950 4078 4197 2561 Size: 1389 128 119 5838 Fragments arranged by size: 5838 1389 128 119 PstI C_TGCA'G Cuts at: 830 3729 3826 3846 4149 4826 830 Size: 2899 97 20 303 677 3478 Fragments arranged by size: 3478 2899 677 303 97 20 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7474 PvuII CAG'CTG Cuts at: 2176 2314 2941 3657 3730 3946 4012 4204 4717 Size: 138 627 716 73 216 66 192 513 Cuts at: 4717 2176 Size: 4933 Fragments arranged by size: 4933 716 627 513 216 192 138 73 66 SacII CC_GC'GG Cuts at: 2084 2084 Size: 7474 SalI G'TCGA_C Cuts at: 1936 4816 1936 Size: 2880 4594 SapI GCTCTTCn'nnn_ Cuts at: 2691 2691 Size: 7474 SbfI CC_TGCA'GG Cuts at: 4826 4826 Size: 7474 ScaI AGT'ACT Cuts at: 4151 5722 7434 4151 Size: 1571 1712 4191 Fragments arranged by size: 4191 1712 1571 SexAI A'CCwGG_T Cuts at: 3489 3981 4050 3489 Size: 492 69 6913 Fragments arranged by size: 6913 492 69 SfcI C'TryA_G Cuts at: 826 1045 3725 3822 3842 4145 4822 5957 6638 Size: 219 2680 97 20 303 677 1135 681 Cuts at: 6638 6829 826 Size: 191 1471 Fragments arranged by size: 2680 1471 1135 681 677 303 219 191 97 20 SfoI GGC'GCC Cuts at: 3121 3121 Size: 7474 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7474 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7474 SmaI CCC'GGG Cuts at: 1872 2560 2966 4196 4801 1872 Size: 688 406 1230 605 4545 Fragments arranged by size: 4545 1230 688 605 406 SmlI C'TyrA_G Cuts at: 820 1017 1943 4238 4364 5578 6479 6741 820 Size: 197 926 2295 126 1214 901 262 1553 Fragments arranged by size: 2295 1553 1214 926 901 262 197 126 SnaBI TAC'GTA Cuts at: 493 5265 493 Size: 4772 2702 SpeI A'CTAG_T Cuts at: 152 4498 152 Size: 4346 3128 SphI G_CATG'C Cuts at: 1132 4832 1132 Size: 3700 3774 SspI AAT'ATT Cuts at: 5 52 5398 5 Size: 47 5346 2081 Fragments arranged by size: 5346 2081 47 StuI AGG'CCT Cuts at: 1517 2279 1517 Size: 762 6712 StyI C'CwwG_G Cuts at: 513 1065 2890 3213 3792 3993 4913 513 Size: 552 1825 323 579 201 920 3074 Fragments arranged by size: 3074 1825 920 579 552 323 201 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2547 3409 4144 5501 5518 5671 5856 Size: 1255 967 862 735 1357 17 153 185 Cuts at: 5856 6301 325 Size: 445 1498 Fragments arranged by size: 1498 1357 1255 967 862 735 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4149 4353 5720 Size: 274 80 33 51 1443 2172 204 1367 Cuts at: 5720 7432 96 Size: 1712 138 Fragments arranged by size: 2172 1712 1443 1367 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4669 4701 7434 7466 4669 Size: 32 2733 32 4677 Fragments arranged by size: 4677 2733 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7474 XbaI T'CTAG_A Cuts at: 4810 4810 Size: 7474 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4111 4695 4111 Size: 584 6890 XhoI C'TCGA_G Cuts at: 1943 4364 1943 Size: 2421 5053 XmaI C'CCGG_G Cuts at: 1870 2558 2964 4194 4799 1870 Size: 688 406 1230 605 4545 Fragments arranged by size: 4545 1230 688 605 406 XmnI GAAnn'nnTTC Cuts at: 5603 5603 Size: 7474 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5282 276 Size: 53 83 186 936 3748 2468 Fragments arranged by size: 3748 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BsiWI EcoICRI EcoRV FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SacI SanDI SfiI SrfI SwaI