(Circular) (Six-base) MAPSORT of: pFN21AB3304.seq Check: 9579 from: 1 to: 5848 pFN21AB3304 5848 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3658 278 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2316 3191 1937 Size: 379 875 4594 Fragments arranged by size: 4594 875 379 Acc65I G'GTAC_C Cuts at: 3169 3169 Size: 5848 AclI AA'CG_TT Cuts at: 1563 3975 4348 1563 Size: 2412 373 3063 Fragments arranged by size: 3063 2412 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3911 5295 5563 1632 Size: 2279 1384 268 1917 Fragments arranged by size: 2279 1917 1384 268 AfeI AGC'GCT Cuts at: 2091 2308 2091 Size: 217 5631 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5651 AflIII A'CryG_T Cuts at: 1203 3073 4747 5724 1203 Size: 1870 1674 977 1327 Fragments arranged by size: 1870 1674 1327 977 AgeI A'CCGG_T Cuts at: 4679 4679 Size: 5848 AhdI GACnn_n'nnGTC Cuts at: 4577 4577 Size: 5848 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5848 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5848 AlwNI CAG_nnn'CTG Cuts at: 2206 2645 2906 3591 5163 2206 Size: 439 261 685 1572 2891 Fragments arranged by size: 2891 1572 685 439 261 ApaLI G'TGCA_C Cuts at: 2119 3905 5061 2119 Size: 1786 1156 2906 Fragments arranged by size: 2906 1786 1156 ApoI r'AATT_y Cuts at: 1493 2747 3157 3383 3535 5644 1493 Size: 1254 410 226 152 2109 1697 Fragments arranged by size: 2109 1697 1254 410 226 152 AseI AT'TA_AT Cuts at: 160 1032 4402 160 Size: 872 3370 1606 Fragments arranged by size: 3370 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 AvaI C'yCGr_G Cuts at: 1870 1943 3173 1870 Size: 73 1230 4545 Fragments arranged by size: 4545 1230 73 BamHI G'GATC_C Cuts at: 3178 3545 3178 Size: 367 5481 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3169 4624 618 Size: 325 217 627 1382 1455 1842 Fragments arranged by size: 1842 1455 1382 627 325 217 BanII G_rGCy'C Cuts at: 1391 2064 3167 1391 Size: 673 1103 4072 Fragments arranged by size: 4072 1103 673 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4877 BbvCI CC'TCA_GC Cuts at: 2310 2310 Size: 5848 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4039 4073 1903 Size: 34 2102 34 3678 Fragments arranged by size: 3678 2102 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3740 4950 3740 Size: 1210 4638 BclI T'GATC_A Cuts at: 1548 2536 1548 Size: 988 4860 BfrBI ATG'CAT Cuts at: 2813 2813 Size: 5848 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4459 136 Size: 107 122 71 631 605 2787 1525 Fragments arranged by size: 2787 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5843 5843 Size: 5848 BlpI GC'TnA_GC Cuts at: 3265 3265 Size: 5848 Bme1580I G_kGCm'C Cuts at: 1790 2123 3909 5065 1790 Size: 333 1786 1156 2573 Fragments arranged by size: 2573 1786 1156 333 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5848 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2264 3579 4537 Size: 416 53 83 399 24 847 1315 958 Cuts at: 4537 442 Size: 1753 Fragments arranged by size: 1753 1315 958 847 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4508 1239 Size: 3269 2579 Bpu10I CC'TnA_GC Cuts at: 2310 2310 Size: 5848 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2218 3973 4838 5136 2218 Size: 1755 865 298 2930 Fragments arranged by size: 2930 1755 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2131 2476 4511 882 Size: 617 632 345 2035 2219 Fragments arranged by size: 2219 2035 632 617 345 BsaAI yAC'GTr Cuts at: 493 1204 3639 493 Size: 711 2435 2702 Fragments arranged by size: 2702 2435 711 BsaBI GATnn'nnATC Cuts at: 5842 5842 Size: 5848 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3655 4037 4672 275 Size: 53 83 186 936 2122 382 635 1451 Fragments arranged by size: 2122 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4281 4679 4953 5100 5511 4281 Size: 398 274 147 411 4618 Fragments arranged by size: 4618 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2478 2508 1178 Size: 30 165 30 1075 30 4518 Fragments arranged by size: 4518 1075 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2198 2244 2247 1186 Size: 862 150 46 3 4787 Fragments arranged by size: 4787 862 150 46 3 BseYI C'CCAG_C Cuts at: 5051 5547 5051 Size: 496 5352 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2132 2846 2876 1163 Size: 573 396 714 30 4135 Fragments arranged by size: 4135 714 573 396 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2686 3298 4059 5087 1540 Size: 275 186 685 612 761 1028 2301 Fragments arranged by size: 2301 1028 761 685 612 275 186 BsiHKAI G_wGCw'C Cuts at: 2064 2123 3909 3994 5065 2064 Size: 59 1786 85 1071 2847 Fragments arranged by size: 2847 1786 1071 85 59 BsmI GAATG_Cn' Cuts at: 2147 3359 3452 2147 Size: 1212 93 4543 Fragments arranged by size: 4543 1212 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2540 2540 Size: 5848 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2123 3167 3909 3994 5065 1391 Size: 399 274 59 1044 742 85 1071 2174 Fragments arranged by size: 2174 1071 1044 742 399 274 85 59 BspEI T'CCGG_A Cuts at: 5511 5511 Size: 5848 BspHI T'CATG_A Cuts at: 3737 5467 3737 Size: 1730 4118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3203 844 Size: 2359 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3735 1394 Size: 429 1912 3507 Fragments arranged by size: 3507 1912 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4343 4517 65 Size: 1183 3095 174 1396 Fragments arranged by size: 3095 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4492 4679 1700 Size: 110 2682 187 2869 Fragments arranged by size: 2869 2682 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5848 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5848 BssSI C'ACGA_G Cuts at: 3006 3027 3908 4920 3006 Size: 21 881 1012 3934 Fragments arranged by size: 3934 1012 881 21 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5848 BstBI TT'CG_AA Cuts at: 4652 4652 Size: 5848 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5848 BstXI CCAn_nnnn'nTGG Cuts at: 2272 2272 Size: 5848 BstYI r'GATC_y Cuts at: 1972 3178 3545 3930 3947 5399 5485 5497 5843 Size: 1206 367 385 17 1452 86 12 346 Cuts at: 5843 1972 Size: 1977 Fragments arranged by size: 1977 1452 1206 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2153 2153 Size: 5848 BtgI C'CryG_G Cuts at: 513 1065 2004 2142 513 Size: 552 939 138 4219 Fragments arranged by size: 4219 939 552 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5109 Fragments arranged by size: 5109 683 56 BtsI GCAGTG_nn' Cuts at: 2121 3364 4158 4178 2121 Size: 1243 794 20 3791 Fragments arranged by size: 3791 1243 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5848 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3004 3039 529 Size: 35 2440 35 3338 Fragments arranged by size: 3338 2440 35 35 DraI TTT'AAA Cuts at: 3152 3152 Size: 5848 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4855 809 Size: 507 3539 1802 Fragments arranged by size: 3539 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2379 3295 4184 Size: 54 1425 50 275 18 549 916 889 Cuts at: 4184 5813 8 Size: 1629 43 Fragments arranged by size: 1629 1425 916 889 549 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3295 1537 Size: 275 1483 4090 Fragments arranged by size: 4090 1483 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2928 3778 1353 Size: 1575 850 3423 Fragments arranged by size: 3423 1575 850 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4435 4809 4955 387 Size: 1064 138 2846 374 146 1280 Fragments arranged by size: 2846 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2062 2062 Size: 5848 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3911 4508 5295 5563 1239 Size: 393 2279 597 787 268 1524 Fragments arranged by size: 2279 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2960 2960 Size: 5848 EcoRI G'AATT_C Cuts at: 2747 3157 3535 2747 Size: 410 378 5060 Fragments arranged by size: 5060 410 378 FspI TGC'GCA Cuts at: 4354 4354 Size: 5848 HaeII r_GCGC'y Cuts at: 1678 2093 2310 4995 1678 Size: 415 217 2685 2531 Fragments arranged by size: 2685 2531 415 217 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2188 2220 2239 2271 2286 Size: 32 135 32 616 32 19 32 15 Cuts at: 2286 2318 2321 2353 2986 3018 4489 4521 4563 Size: 32 3 32 633 32 1471 32 42 Cuts at: 4563 4595 1373 Size: 32 2626 Fragments arranged by size: 2626 1471 633 616 135 42 32 32 32 32 32 32 32 32 32 19 15 3 HincII GTy'rAC Cuts at: 669 1657 1938 2563 3192 3438 669 Size: 988 281 625 629 246 3079 Fragments arranged by size: 3079 988 629 625 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5848 HpaI GTT'AAC Cuts at: 2563 3438 2563 Size: 875 4973 KpnI G_GTAC'C Cuts at: 3173 3173 Size: 5848 MfeI C'AATT_G Cuts at: 3447 3447 Size: 5848 MluI A'CGCG_T Cuts at: 5724 5724 Size: 5848 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2154 4962 5146 1305 Size: 849 2808 184 2007 Fragments arranged by size: 2808 2007 849 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4026 Fragments arranged by size: 4026 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2427 3806 4165 4324 518 Size: 648 1261 1379 359 159 2042 Fragments arranged by size: 2042 1379 1261 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2903 3264 3941 5089 5334 1606 Size: 1297 361 677 1148 245 2120 Fragments arranged by size: 2120 1297 1148 677 361 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2142 513 Size: 552 939 138 4219 Fragments arranged by size: 4219 939 552 138 NdeI CA'TA_TG Cuts at: 387 387 Size: 5848 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5848 NotI GC'GGCC_GC Cuts at: 3295 3295 Size: 5848 NsiI A_TGCA'T Cuts at: 2815 2815 Size: 5848 NspI r_CATG'y Cuts at: 1132 1229 3206 4751 1132 Size: 97 1977 1545 2229 Fragments arranged by size: 2229 1977 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5848 PciI A'CATG_T Cuts at: 4747 4747 Size: 5848 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5848 PmeI GTTT'AAAC Cuts at: 3152 3152 Size: 5848 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3917 3949 5430 5462 3917 Size: 32 1481 32 4303 Fragments arranged by size: 4303 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2552 1613 Size: 939 4909 PsiI TTA'TAA Cuts at: 3418 3418 Size: 5848 PstI C_TGCA'G Cuts at: 830 2211 3200 830 Size: 1381 989 3478 Fragments arranged by size: 3478 1381 989 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5848 PvuII CAG'CTG Cuts at: 2903 2903 Size: 5848 SacI G_AGCT'C Cuts at: 2064 2064 Size: 5848 SalI G'TCGA_C Cuts at: 1936 3190 1936 Size: 1254 4594 SbfI CC_TGCA'GG Cuts at: 3200 3200 Size: 5848 ScaI AGT'ACT Cuts at: 4096 5808 4096 Size: 1712 4136 SfcI C'TryA_G Cuts at: 826 1045 2207 2695 3196 4331 5012 5203 826 Size: 219 1162 488 501 1135 681 191 1471 Fragments arranged by size: 1471 1162 1135 681 501 488 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5848 SmaI CCC'GGG Cuts at: 1872 3175 1872 Size: 1303 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2233 3952 4853 5115 820 Size: 197 926 290 1719 901 262 1553 Fragments arranged by size: 1719 1553 926 901 290 262 197 SnaBI TAC'GTA Cuts at: 493 3639 493 Size: 3146 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5848 SphI G_CATG'C Cuts at: 1132 3206 1132 Size: 2074 3774 SspI AAT'ATT Cuts at: 5 52 3772 5 Size: 47 3720 2081 Fragments arranged by size: 3720 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5848 StyI C'CwwG_G Cuts at: 513 1065 2004 2142 3287 513 Size: 552 939 138 1145 3074 Fragments arranged by size: 3074 1145 939 552 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2278 2458 2700 2910 3072 3875 3892 Size: 1255 698 180 242 210 162 803 17 Cuts at: 3892 4045 4230 4675 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 803 698 445 242 210 185 180 162 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3120 4094 5806 Size: 274 80 33 51 1443 1143 974 1712 Cuts at: 5806 96 Size: 138 Fragments arranged by size: 1712 1443 1143 974 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2478 2510 5808 5840 2478 Size: 32 3298 32 2486 Fragments arranged by size: 3298 2486 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5848 XbaI T'CTAG_A Cuts at: 3184 3184 Size: 5848 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5848 XmaI C'CCGG_G Cuts at: 1870 3173 1870 Size: 1303 4545 XmnI GAAnn'nnTTC Cuts at: 3075 3977 3075 Size: 902 4946 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3656 276 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI