(Circular) (Six-base) MAPSORT of: pFN21AB3287.seq Check: 9392 from: 1 to: 7573 pFN21AB3287 7573 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5383 278 Size: 53 83 186 936 3847 2468 Fragments arranged by size: 3847 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4916 1937 Size: 2979 4594 Acc65I G'GTAC_C Cuts at: 4894 4894 Size: 7573 AclI AA'CG_TT Cuts at: 1563 5700 6073 1563 Size: 4137 373 3063 Fragments arranged by size: 4137 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2711 3317 4361 5636 7020 7288 1632 Size: 1079 606 1044 1275 1384 268 1917 Fragments arranged by size: 1917 1384 1275 1079 1044 606 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7376 AflIII A'CryG_T Cuts at: 1203 4083 6472 7449 1203 Size: 2880 2389 977 1327 Fragments arranged by size: 2880 2389 1327 977 AgeI A'CCGG_T Cuts at: 6404 6404 Size: 7573 AhdI GACnn_n'nnGTC Cuts at: 2569 6302 2569 Size: 3733 3840 AleI CACnn'nnGTG Cuts at: 1166 3738 1166 Size: 2572 5001 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3971 1184 Size: 2787 4786 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3761 3793 3761 Size: 32 7541 AlwNI CAG_nnn'CTG Cuts at: 2968 3795 4717 4795 5316 6888 2968 Size: 827 922 78 521 1572 3653 Fragments arranged by size: 3653 1572 922 827 521 78 ApaLI G'TGCA_C Cuts at: 5630 6786 5630 Size: 1156 6417 ApoI r'AATT_y Cuts at: 1493 3095 4045 4147 4882 5108 5260 7369 1493 Size: 1602 950 102 735 226 152 2109 1697 Fragments arranged by size: 2109 1697 1602 950 735 226 152 102 AscI GG'CGCG_CC Cuts at: 4597 4597 Size: 7573 AseI AT'TA_AT Cuts at: 160 1032 6127 160 Size: 872 5095 1606 Fragments arranged by size: 5095 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7573 AvaI C'yCGr_G Cuts at: 1870 1943 2595 3431 4631 4772 4868 4898 1870 Size: 73 652 836 1200 141 96 30 4545 Fragments arranged by size: 4545 1200 836 652 141 96 73 30 BamHI G'GATC_C Cuts at: 2460 4903 5270 2460 Size: 2443 367 4763 Fragments arranged by size: 4763 2443 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2020 3035 3443 4894 6349 Size: 325 217 627 233 1015 408 1451 1455 Cuts at: 6349 618 Size: 1842 Fragments arranged by size: 1842 1455 1451 1015 627 408 325 233 217 BanII G_rGCy'C Cuts at: 1391 3830 4698 4869 4892 1391 Size: 2439 868 171 23 4072 Fragments arranged by size: 4072 2439 868 171 23 BbeI G_GCGC'C Cuts at: 2024 2024 Size: 7573 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6602 BbvCI CC'TCA_GC Cuts at: 3197 3197 Size: 7573 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4249 4283 4791 4825 5764 Size: 34 50 34 2228 34 508 34 939 Cuts at: 5764 5798 1903 Size: 34 3678 Fragments arranged by size: 3678 2228 939 508 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4589 5465 6675 4589 Size: 876 1210 5487 Fragments arranged by size: 5487 1210 876 BclI T'GATC_A Cuts at: 1548 3894 1548 Size: 2346 5227 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3671 3923 4618 Size: 107 122 71 631 605 1999 252 695 Cuts at: 4618 4739 6184 136 Size: 121 1445 1525 Fragments arranged by size: 1999 1525 1445 695 631 605 252 122 121 107 71 BglII A'GATC_T Cuts at: 3021 3487 7568 3021 Size: 466 4081 3026 Fragments arranged by size: 4081 3026 466 BlpI GC'TnA_GC Cuts at: 4664 4990 4664 Size: 326 7247 Bme1580I G_kGCm'C Cuts at: 1790 2895 3745 3960 4373 4655 4804 5634 6790 Size: 1105 850 215 413 282 149 830 1156 Cuts at: 6790 1790 Size: 2573 Fragments arranged by size: 2573 1156 1105 850 830 413 282 215 149 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7573 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2910 5304 6262 Size: 416 53 83 399 24 1493 2394 958 Cuts at: 6262 442 Size: 1753 Fragments arranged by size: 2394 1753 1493 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7573 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2149 2149 Size: 7573 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2296 2744 2768 4009 4292 4352 4856 6233 Size: 1057 448 24 1241 283 60 504 1377 Cuts at: 6233 1239 Size: 2579 Fragments arranged by size: 2579 1377 1241 1057 504 448 283 60 24 Bpu10I CC'TnA_GC Cuts at: 3197 3197 Size: 7573 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2098 2600 3344 5698 6563 6861 2098 Size: 502 744 2354 865 298 2810 Fragments arranged by size: 2810 2354 865 744 502 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6236 882 Size: 617 4737 2219 Fragments arranged by size: 4737 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3184 5364 493 Size: 711 1980 2180 2702 Fragments arranged by size: 2702 2180 1980 711 BsaBI GATnn'nnATC Cuts at: 3899 7567 3899 Size: 3668 3905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2021 3642 3710 3873 Size: 53 83 186 936 488 1621 68 163 Cuts at: 3873 5380 5762 6397 275 Size: 1507 382 635 1451 Fragments arranged by size: 1621 1507 1451 936 635 488 382 186 163 83 68 53 BsaWI w'CCGG_w Cuts at: 2973 3802 6006 6404 6678 6825 7236 2973 Size: 829 2204 398 274 147 411 3310 Fragments arranged by size: 3310 2204 829 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3761 3791 4007 4037 1178 Size: 30 165 30 2358 30 216 30 4714 Fragments arranged by size: 4714 2358 216 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2390 2603 2905 4139 4649 4841 1186 Size: 1204 213 302 1234 510 192 3918 Fragments arranged by size: 3918 1234 1204 510 302 213 192 BseYI C'CCAG_C Cuts at: 2179 2305 2426 2456 4100 6776 7272 2179 Size: 126 121 30 1644 2676 496 2480 Fragments arranged by size: 2676 2480 1644 496 126 121 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2134 2464 2981 3962 4735 1163 Size: 573 398 330 517 981 773 4001 Fragments arranged by size: 4001 981 773 573 517 398 330 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3807 4646 4808 5023 5784 6812 Size: 275 186 1806 839 162 215 761 1028 Cuts at: 6812 1540 Size: 2301 Fragments arranged by size: 2301 1806 1028 839 761 275 215 186 162 BsiHKAI G_wGCw'C Cuts at: 4121 5634 5719 6790 4121 Size: 1513 85 1071 4904 Fragments arranged by size: 4904 1513 1071 85 BsmI GAATG_Cn' Cuts at: 2519 3573 3884 5084 5177 2519 Size: 1054 311 1200 93 4915 Fragments arranged by size: 4915 1200 1054 311 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2020 2020 Size: 7573 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2895 3745 3830 3960 4121 4373 4655 Size: 399 1105 850 85 130 161 252 282 Cuts at: 4655 4698 4804 4869 4892 5634 5719 6790 1391 Size: 43 106 65 23 742 85 1071 2174 Fragments arranged by size: 2174 1105 1071 850 742 399 282 252 161 130 106 85 85 65 43 23 BspEI T'CCGG_A Cuts at: 7236 7236 Size: 7573 BspHI T'CATG_A Cuts at: 3045 3555 5462 7192 3045 Size: 510 1907 1730 3426 Fragments arranged by size: 3426 1907 1730 510 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3063 4928 844 Size: 2219 1865 3489 Fragments arranged by size: 3489 2219 1865 BsrBI CCG'CTC Cuts at: 1394 1823 2128 2611 5460 1394 Size: 429 305 483 2849 3507 Fragments arranged by size: 3507 2849 483 429 305 BsrDI GCAATG_nn' Cuts at: 65 1248 3331 6068 6242 65 Size: 1183 2083 2737 174 1396 Fragments arranged by size: 2737 2083 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3083 3750 4590 6217 6404 1700 Size: 110 1273 667 840 1627 187 2869 Fragments arranged by size: 2869 1627 1273 840 667 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7573 BssHII G'CGCG_C Cuts at: 1925 4597 1925 Size: 2672 4901 BssSI C'ACGA_G Cuts at: 2048 5633 6645 2048 Size: 3585 1012 2976 Fragments arranged by size: 3585 2976 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7573 BstBI TT'CG_AA Cuts at: 6377 6377 Size: 7573 BstEII G'GTnAC_C Cuts at: 1184 4540 1184 Size: 3356 4217 BstXI CCAn_nnnn'nTGG Cuts at: 2143 2275 3274 4468 2143 Size: 132 999 1194 5248 Fragments arranged by size: 5248 1194 999 132 BstYI r'GATC_y Cuts at: 1972 2259 2460 2622 3021 3375 3396 3487 4335 Size: 287 201 162 399 354 21 91 848 Cuts at: 4335 4903 5270 5655 5672 7124 7210 7222 7568 Size: 568 367 385 17 1452 86 12 346 Cuts at: 7568 1972 Size: 1977 Fragments arranged by size: 1977 1452 848 568 399 385 367 354 346 287 201 162 91 86 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 2106 4646 4744 513 Size: 552 1041 2540 98 3342 Fragments arranged by size: 3342 2540 1041 552 98 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3820 487 Size: 683 56 2594 4240 Fragments arranged by size: 4240 2594 683 56 BtsI GCAGTG_nn' Cuts at: 5089 5883 5903 5089 Size: 794 20 6759 Fragments arranged by size: 6759 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7573 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2317 2352 4055 4090 529 Size: 35 1753 35 1703 35 4012 Fragments arranged by size: 4012 1753 1703 35 35 35 DraI TTT'AAA Cuts at: 2523 4877 2523 Size: 2354 5219 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6580 809 Size: 507 5264 1802 Fragments arranged by size: 5264 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 3085 3172 Size: 54 1425 50 275 18 315 940 87 Cuts at: 3172 3672 3915 4299 4458 4805 5020 5909 7538 Size: 500 243 384 159 347 215 889 1629 Cuts at: 7538 8 Size: 43 Fragments arranged by size: 1629 1425 940 889 500 384 347 315 275 243 215 159 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4805 5020 1537 Size: 275 2993 215 4090 Fragments arranged by size: 4090 2993 275 215 EarI CTCTTCn'nnn_ Cuts at: 1353 3248 3656 3695 4109 5503 1353 Size: 1895 408 39 414 1394 3423 Fragments arranged by size: 3423 1895 1394 414 408 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3389 6160 6534 6680 387 Size: 1064 138 1800 2771 374 146 1280 Fragments arranged by size: 2771 1800 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2296 2711 2744 2768 3317 4009 4292 Size: 393 664 415 33 24 549 692 283 Cuts at: 4292 4352 4361 4856 5636 6233 7020 7288 1239 Size: 60 9 495 780 597 787 268 1524 Fragments arranged by size: 1524 787 780 692 664 597 549 495 415 393 283 268 60 33 24 9 EcoNI CCTnn'n_nnAGG Cuts at: 2267 2690 4568 2267 Size: 423 1878 5272 Fragments arranged by size: 5272 1878 423 EcoO109I rG'GnC_Cy Cuts at: 2350 2599 3193 4228 4792 2350 Size: 249 594 1035 564 5131 Fragments arranged by size: 5131 1035 594 564 249 EcoRI G'AATT_C Cuts at: 3095 4147 4882 5260 3095 Size: 1052 735 378 5408 Fragments arranged by size: 5408 1052 735 378 FspI TGC'GCA Cuts at: 3059 4553 6079 3059 Size: 1494 1526 4553 Fragments arranged by size: 4553 1526 1494 HaeII r_GCGC'y Cuts at: 1678 2024 4642 6720 1678 Size: 346 2618 2078 2531 Fragments arranged by size: 2618 2531 2078 346 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2555 2587 6214 6246 6288 Size: 32 135 32 983 32 3627 32 42 Cuts at: 6288 6320 1373 Size: 32 2626 Fragments arranged by size: 3627 2626 983 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2794 3708 4917 5163 669 Size: 988 281 856 914 1209 246 3079 Fragments arranged by size: 3079 1209 988 914 856 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7573 HpaI GTT'AAC Cuts at: 5163 5163 Size: 7573 KasI G'GCGC_C Cuts at: 2020 2020 Size: 7573 KpnI G_GTAC'C Cuts at: 4898 4898 Size: 7573 MfeI C'AATT_G Cuts at: 5172 5172 Size: 7573 MluI A'CGCG_T Cuts at: 7449 7449 Size: 7573 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3144 3249 4176 4458 6687 6871 1305 Size: 1839 105 927 282 2229 184 2007 Fragments arranged by size: 2229 2007 1839 927 282 184 105 MscI TGG'CCA Cuts at: 10 64 1489 1832 2147 4301 4460 10 Size: 54 1425 343 315 2154 159 3123 Fragments arranged by size: 3123 2154 1425 343 315 159 54 MslI CAynn'nnrTG Cuts at: 518 1166 2444 3216 3736 3738 3953 4466 5531 Size: 648 1278 772 520 2 215 513 1065 Cuts at: 5531 5890 6049 518 Size: 359 159 2042 Fragments arranged by size: 2042 1278 1065 772 648 520 513 359 215 159 2 MspA1I CmG'CkG Cuts at: 1606 3518 3681 3861 4312 4648 4746 4989 5666 Size: 1912 163 180 451 336 98 243 677 Cuts at: 5666 6814 7059 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1912 1148 677 451 336 245 243 180 163 98 NaeI GCC'GGC Cuts at: 4592 4592 Size: 7573 NarI GG'CG_CC Cuts at: 2021 2021 Size: 7573 NcoI C'CATG_G Cuts at: 513 1065 2106 513 Size: 552 1041 5980 Fragments arranged by size: 5980 1041 552 NdeI CA'TA_TG Cuts at: 387 3591 387 Size: 3204 4369 NgoMIV G'CCGG_C Cuts at: 4590 4590 Size: 7573 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7573 NotI GC'GGCC_GC Cuts at: 5020 5020 Size: 7573 NspI r_CATG'y Cuts at: 1132 1229 2584 3358 4075 4087 4264 4931 6476 Size: 97 1355 774 717 12 177 667 1545 Cuts at: 6476 1132 Size: 2229 Fragments arranged by size: 2229 1545 1355 774 717 667 177 97 12 PasI CC'CwG_GG Cuts at: 1348 3745 3781 1348 Size: 2397 36 5140 Fragments arranged by size: 5140 2397 36 PciI A'CATG_T Cuts at: 4083 6472 4083 Size: 2389 5184 PflMI CCAn_nnn'nTGG Cuts at: 1108 2767 3316 4537 1108 Size: 1659 549 1221 4144 Fragments arranged by size: 4144 1659 1221 549 PfoI T'CCnGG_A Cuts at: 2625 3273 3621 4167 4281 2625 Size: 648 348 546 114 5917 Fragments arranged by size: 5917 648 546 348 114 PmeI GTTT'AAAC Cuts at: 4877 4877 Size: 7573 PmlI CAC'GTG Cuts at: 3184 3184 Size: 7573 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3761 3793 5642 5674 7155 7187 3761 Size: 32 1849 32 1481 32 4147 Fragments arranged by size: 4147 1849 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2350 2599 2350 Size: 249 7324 PshAI GACnn'nnGTC Cuts at: 1613 2296 1613 Size: 683 6890 PsiI TTA'TAA Cuts at: 5143 5143 Size: 7573 PstI C_TGCA'G Cuts at: 830 2066 2245 4317 4925 830 Size: 1236 179 2072 608 3478 Fragments arranged by size: 3478 2072 1236 608 179 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7573 PvuII CAG'CTG Cuts at: 3518 3681 4312 3518 Size: 163 631 6779 Fragments arranged by size: 6779 631 163 SacII CC_GC'GG Cuts at: 4649 4747 4649 Size: 98 7475 SalI G'TCGA_C Cuts at: 1936 4915 1936 Size: 2979 4594 SanDI GG'GwC_CC Cuts at: 2599 2599 Size: 7573 SapI GCTCTTCn'nnn_ Cuts at: 3656 4109 3656 Size: 453 7120 SbfI CC_TGCA'GG Cuts at: 4925 4925 Size: 7573 ScaI AGT'ACT Cuts at: 3776 4190 5821 7533 3776 Size: 414 1631 1712 3816 Fragments arranged by size: 3816 1712 1631 414 SfcI C'TryA_G Cuts at: 826 1045 2062 2241 2451 4313 4921 6056 6737 Size: 219 1017 179 210 1862 608 1135 681 Cuts at: 6737 6928 826 Size: 191 1471 Fragments arranged by size: 1862 1471 1135 1017 681 608 219 210 191 179 SfoI GGC'GCC Cuts at: 2022 2022 Size: 7573 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7573 SgrAI Cr'CCGG_yG Cuts at: 1700 4590 1700 Size: 2890 4683 SmaI CCC'GGG Cuts at: 1872 2597 4900 1872 Size: 725 2303 4545 Fragments arranged by size: 4545 2303 725 SmlI C'TyrA_G Cuts at: 820 1017 1943 2077 2615 3359 5677 6578 6840 Size: 197 926 134 538 744 2318 901 262 Cuts at: 6840 820 Size: 1553 Fragments arranged by size: 2318 1553 926 901 744 538 262 197 134 SnaBI TAC'GTA Cuts at: 493 5364 493 Size: 4871 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7573 SphI G_CATG'C Cuts at: 1132 2584 4264 4931 1132 Size: 1452 1680 667 3774 Fragments arranged by size: 3774 1680 1452 667 SspI AAT'ATT Cuts at: 5 52 4417 5497 5 Size: 47 4365 1080 2081 Fragments arranged by size: 4365 2081 1080 47 StuI AGG'CCT Cuts at: 1517 3504 4401 1517 Size: 1987 897 4689 Fragments arranged by size: 4689 1987 897 StyI C'CwwG_G Cuts at: 513 1065 2106 2190 2268 2559 4242 4374 5012 Size: 552 1041 84 78 291 1683 132 638 Cuts at: 5012 513 Size: 3074 Fragments arranged by size: 3074 1683 1041 638 552 291 132 84 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3750 4559 5600 5617 5770 5955 6400 Size: 1255 2170 809 1041 17 153 185 445 Cuts at: 6400 325 Size: 1498 Fragments arranged by size: 2170 1498 1255 1041 809 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3774 4188 5819 Size: 274 80 33 51 1443 1797 414 1631 Cuts at: 5819 7531 96 Size: 1712 138 Fragments arranged by size: 1797 1712 1631 1443 414 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4007 4039 4416 4448 7533 7565 4007 Size: 32 377 32 3085 32 4015 Fragments arranged by size: 4015 3085 377 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7573 XbaI T'CTAG_A Cuts at: 4909 4909 Size: 7573 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2197 3967 2197 Size: 1770 5803 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7573 XmaI C'CCGG_G Cuts at: 1870 2595 4898 1870 Size: 725 2303 4545 Fragments arranged by size: 4545 2303 725 XmnI GAAnn'nnTTC Cuts at: 2471 5702 2471 Size: 3231 4342 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5381 276 Size: 53 83 186 936 3847 2468 Fragments arranged by size: 3847 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI