(Circular) (Six-base) MAPSORT of: pFN21AB3283.seq Check: 7079 from: 1 to: 8272 pFN21AB3283 8272 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2495 2721 3684 5505 2495 Size: 226 963 1821 5262 Fragments arranged by size: 5262 1821 963 226 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6082 278 Size: 53 83 186 936 4546 2468 Fragments arranged by size: 4546 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2565 5615 1937 Size: 628 3050 4594 Fragments arranged by size: 4594 3050 628 Acc65I G'GTAC_C Cuts at: 2913 5593 2913 Size: 2680 5592 AclI AA'CG_TT Cuts at: 1563 6399 6772 1563 Size: 4836 373 3063 Fragments arranged by size: 4836 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2048 2584 3161 3434 6335 7719 7987 1632 Size: 416 536 577 273 2901 1384 268 1917 Fragments arranged by size: 2901 1917 1384 577 536 416 273 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8075 AflIII A'CryG_T Cuts at: 1203 5169 7171 8148 1203 Size: 3966 2002 977 1327 Fragments arranged by size: 3966 2002 1327 977 AgeI A'CCGG_T Cuts at: 7103 7103 Size: 8272 AhdI GACnn_n'nnGTC Cuts at: 7001 7001 Size: 8272 AleI CACnn'nnGTG Cuts at: 1166 2941 1166 Size: 1775 6497 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4049 5072 1184 Size: 2865 1023 4384 Fragments arranged by size: 4384 2865 1023 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4413 4445 4413 Size: 32 8240 AlwNI CAG_nnn'CTG Cuts at: 2822 3114 3317 3896 4705 5392 6015 7587 2822 Size: 292 203 579 809 687 623 1572 3507 Fragments arranged by size: 3507 1572 809 687 623 579 292 203 ApaI G_GGCC'C Cuts at: 2210 2246 3235 3745 4105 4260 2210 Size: 36 989 510 360 155 6222 Fragments arranged by size: 6222 989 510 360 155 36 ApaLI G'TGCA_C Cuts at: 3946 6329 7485 3946 Size: 2383 1156 4733 Fragments arranged by size: 4733 2383 1156 ApoI r'AATT_y Cuts at: 1493 5581 5807 5959 8068 1493 Size: 4088 226 152 2109 1697 Fragments arranged by size: 4088 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6826 160 Size: 872 5794 1606 Fragments arranged by size: 5794 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8272 AvaI C'yCGr_G Cuts at: 1870 1943 2100 3254 3385 3442 4092 4106 4468 Size: 73 157 1154 131 57 650 14 362 Cuts at: 4468 5597 1870 Size: 1129 4545 Fragments arranged by size: 4545 1154 1129 650 362 157 131 73 57 14 BamHI G'GATC_C Cuts at: 2632 4632 5602 5969 2632 Size: 2000 970 367 4935 Fragments arranged by size: 4935 2000 970 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 2479 2709 2913 2943 Size: 325 217 627 246 446 230 204 30 Cuts at: 2943 3056 5085 5237 5252 5292 5359 5593 7048 Size: 113 2029 152 15 40 67 234 1455 Cuts at: 7048 618 Size: 1842 Fragments arranged by size: 2029 1842 1455 627 446 325 246 234 230 217 204 152 113 67 40 30 15 BanII G_rGCy'C Cuts at: 1391 2088 2210 2246 2470 2664 3235 3745 4105 Size: 697 122 36 224 194 571 510 360 Cuts at: 4105 4260 4557 4712 4805 4962 4991 5133 5374 Size: 155 297 155 93 157 29 142 241 Cuts at: 5374 5591 1391 Size: 217 4072 Fragments arranged by size: 4072 697 571 510 360 297 241 224 217 194 157 155 155 142 122 93 36 29 BbeI G_GCGC'C Cuts at: 5241 5256 5296 5241 Size: 15 40 8217 Fragments arranged by size: 8217 40 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2057 3152 3600 928 Size: 971 158 1095 448 5600 Fragments arranged by size: 5600 1095 971 448 158 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3646 3680 6463 6497 1903 Size: 34 1709 34 2783 34 3678 Fragments arranged by size: 3678 2783 1709 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6164 7374 6164 Size: 1210 7062 BclI T'GATC_A Cuts at: 1548 1548 Size: 8272 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2729 3042 5083 Size: 107 122 71 631 605 1057 313 2041 Cuts at: 5083 6883 136 Size: 1800 1525 Fragments arranged by size: 2041 1800 1525 1057 631 605 313 122 107 71 BglII A'GATC_T Cuts at: 8267 8267 Size: 8272 BlpI GC'TnA_GC Cuts at: 3653 4022 4706 5454 5689 3653 Size: 369 684 748 235 6236 Fragments arranged by size: 6236 748 684 369 235 Bme1580I G_kGCm'C Cuts at: 1790 2036 2210 2246 2351 2484 2592 2712 2829 Size: 246 174 36 105 133 108 120 117 Cuts at: 2829 2948 3235 3508 3534 3745 3950 3966 4071 Size: 119 287 273 26 211 205 16 105 Cuts at: 4071 4105 4260 4684 5095 5182 5225 6333 7489 Size: 34 155 424 411 87 43 1108 1156 Cuts at: 7489 1790 Size: 2573 Fragments arranged by size: 2573 1156 1108 424 411 287 273 246 211 205 174 155 133 120 119 117 108 105 105 87 43 36 34 26 16 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8272 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3906 3985 4362 Size: 416 53 83 399 24 2489 79 377 Cuts at: 4362 5527 6003 6961 442 Size: 1165 476 958 1753 Fragments arranged by size: 2489 1753 1165 958 476 416 399 377 83 79 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8272 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3582 4783 3582 Size: 1201 7071 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2465 2480 3068 3419 3537 3595 3985 4488 Size: 1226 15 588 351 118 58 390 503 Cuts at: 4488 4621 4976 5195 5264 5318 6932 1239 Size: 133 355 219 69 54 1614 2579 Fragments arranged by size: 2579 1614 1226 588 503 390 355 351 219 133 118 69 58 54 15 Bpu10I CC'TnA_GC Cuts at: 2165 2376 2605 2955 4776 2165 Size: 211 229 350 1821 5661 Fragments arranged by size: 5661 1821 350 229 211 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2381 6397 7262 7560 2381 Size: 4016 865 298 3093 Fragments arranged by size: 4016 3093 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2441 4202 4220 5142 5429 6935 882 Size: 617 942 1761 18 922 287 1506 2219 Fragments arranged by size: 2219 1761 1506 942 922 617 287 18 BsaAI yAC'GTr Cuts at: 493 1204 5170 6063 493 Size: 711 3966 893 2702 Fragments arranged by size: 3966 2702 893 711 BsaBI GATnn'nnATC Cuts at: 8266 8266 Size: 8272 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2160 5187 5238 5253 Size: 53 83 186 936 627 3027 51 15 Cuts at: 5253 5293 6079 6461 7096 275 Size: 40 786 382 635 1451 Fragments arranged by size: 3027 1451 936 786 635 627 382 186 83 53 51 40 15 BsaWI w'CCGG_w Cuts at: 3298 4882 6705 7103 7377 7524 7935 3298 Size: 1584 1823 398 274 147 411 3635 Fragments arranged by size: 3635 1823 1584 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4680 4710 5337 5367 5411 Size: 30 165 30 3277 30 627 30 44 Cuts at: 5411 5441 1178 Size: 30 4009 Fragments arranged by size: 4009 3277 627 165 44 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3221 3540 3956 4220 5318 1186 Size: 2035 319 416 264 1098 4140 Fragments arranged by size: 4140 2035 1098 416 319 264 BseYI C'CCAG_C Cuts at: 2018 2275 2888 2920 2970 3077 3122 3332 3744 Size: 257 613 32 50 107 45 210 412 Cuts at: 3744 4231 4557 4725 4730 4962 5190 5474 7475 Size: 487 326 168 5 232 228 284 2001 Cuts at: 7475 7971 2018 Size: 496 2319 Fragments arranged by size: 2319 2001 613 496 487 412 326 284 257 232 228 210 168 107 50 45 32 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2096 2732 4718 4802 5405 1163 Size: 573 360 636 1986 84 603 4030 Fragments arranged by size: 4030 1986 636 603 573 360 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5229 5722 6483 7511 1540 Size: 275 186 3228 493 761 1028 2301 Fragments arranged by size: 3228 2301 1028 761 493 275 186 BsiHKAI G_wGCw'C Cuts at: 2171 2470 3950 4028 4599 5402 6333 6418 7489 Size: 299 1480 78 571 803 931 85 1071 Cuts at: 7489 2171 Size: 2954 Fragments arranged by size: 2954 1480 1071 931 803 571 299 85 78 BsmI GAATG_Cn' Cuts at: 2330 2640 3789 5783 5876 2330 Size: 310 1149 1994 93 4726 Fragments arranged by size: 4726 1994 1149 310 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2088 2171 2210 2246 2351 2470 Size: 399 246 52 83 39 36 105 119 Cuts at: 2470 2484 2592 2664 2712 2829 2948 3235 3508 Size: 14 108 72 48 117 119 287 273 Cuts at: 3508 3534 3745 3950 3966 4028 4071 4105 4260 Size: 26 211 205 16 62 43 34 155 Cuts at: 4260 4557 4599 4684 4712 4805 4962 4991 5095 Size: 297 42 85 28 93 157 29 104 Cuts at: 5095 5133 5182 5225 5374 5402 5591 6333 6418 Size: 38 49 43 149 28 189 742 85 Cuts at: 6418 7489 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 297 287 273 246 211 205 189 157 155 149 119 119 117 108 105 104 93 85 85 83 72 62 52 49 48 43 43 42 39 38 36 34 29 28 28 26 16 14 BspEI T'CCGG_A Cuts at: 4882 7935 4882 Size: 3053 5219 BspHI T'CATG_A Cuts at: 6161 7891 6161 Size: 1730 6542 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2495 2721 3684 5505 5627 844 Size: 1651 226 963 1821 122 3489 Fragments arranged by size: 3489 1821 1651 963 226 122 BsrBI CCG'CTC Cuts at: 1394 1823 2800 3724 6159 1394 Size: 429 977 924 2435 3507 Fragments arranged by size: 3507 2435 977 924 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3276 4038 4177 4643 6767 6941 65 Size: 1183 2028 762 139 466 2124 174 1396 Fragments arranged by size: 2124 2028 1396 1183 762 466 174 139 BsrFI r'CCGG_y Cuts at: 1700 1810 2778 3214 3648 3861 6916 7103 1700 Size: 110 968 436 434 213 3055 187 2869 Fragments arranged by size: 3055 2869 968 436 434 213 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8272 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8272 BssSI C'ACGA_G Cuts at: 6332 7344 6332 Size: 1012 7260 BstAPI GCAn_nnn'nTGC Cuts at: 794 3349 3810 794 Size: 2555 461 5256 Fragments arranged by size: 5256 2555 461 BstBI TT'CG_AA Cuts at: 7076 7076 Size: 8272 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8272 BstXI CCAn_nnnn'nTGG Cuts at: 2181 2458 2538 2759 3515 4393 4738 4852 2181 Size: 277 80 221 756 878 345 114 5601 Fragments arranged by size: 5601 878 756 345 277 221 114 80 BstYI r'GATC_y Cuts at: 1972 2454 2632 3013 4428 4632 5602 5969 6354 Size: 482 178 381 1415 204 970 367 385 Cuts at: 6354 6371 7823 7909 7921 8267 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1415 970 482 385 381 367 346 204 178 86 17 12 BstZ17I GTA'TAC Cuts at: 2566 2566 Size: 8272 Bsu36I CC'TnA_GG Cuts at: 3198 3198 Size: 8272 BtgI C'CryG_G Cuts at: 513 1065 2004 2116 3817 3936 4299 4900 5547 Size: 552 939 112 1701 119 363 601 647 Cuts at: 5547 513 Size: 3238 Fragments arranged by size: 3238 1701 939 647 601 552 363 119 112 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2913 3856 487 Size: 683 56 1687 943 4903 Fragments arranged by size: 4903 1687 943 683 56 BtsI GCAGTG_nn' Cuts at: 2428 2744 3315 4026 4069 5072 5541 5788 6582 Size: 316 571 711 43 1003 469 247 794 Cuts at: 6582 6602 2428 Size: 20 4098 Fragments arranged by size: 4098 1003 794 711 571 469 316 247 43 20 ClaI AT'CG_AT Cuts at: 1554 3362 1554 Size: 1808 6464 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8237 DraI TTT'AAA Cuts at: 5576 5576 Size: 8272 DraIII CAC_nnn'GTG Cuts at: 5163 5383 5163 Size: 220 8052 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7279 809 Size: 507 5963 1802 Fragments arranged by size: 5963 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2389 2776 3293 Size: 54 1425 50 275 18 559 387 517 Cuts at: 3293 3421 4249 5034 5226 5544 5719 6608 8237 Size: 128 828 785 192 318 175 889 1629 Cuts at: 8237 8 Size: 43 Fragments arranged by size: 1629 1425 889 828 785 559 517 387 318 275 192 175 128 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5226 5719 1537 Size: 275 3414 493 4090 Fragments arranged by size: 4090 3414 493 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2807 3830 4592 6202 1353 Size: 1454 1023 762 1610 3423 Fragments arranged by size: 3423 1610 1454 1023 762 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5189 6859 7233 7379 387 Size: 1064 138 3600 1670 374 146 1280 Fragments arranged by size: 3600 1670 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2468 2468 Size: 8272 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2465 2480 2584 3068 3161 3419 Size: 393 416 417 15 104 484 93 258 Cuts at: 3419 3434 3537 3595 3985 4488 4621 4976 5195 Size: 15 103 58 390 503 133 355 219 Cuts at: 5195 5264 5318 6335 6932 7719 7987 1239 Size: 69 54 1017 597 787 268 1524 Fragments arranged by size: 1524 1017 787 597 503 484 417 416 393 390 355 268 258 219 133 104 103 93 69 58 54 15 15 EcoNI CCTnn'n_nnAGG Cuts at: 2152 3309 3570 4527 4589 5300 5333 2152 Size: 1157 261 957 62 711 33 5091 Fragments arranged by size: 5091 1157 957 711 261 62 33 EcoO109I rG'GnC_Cy Cuts at: 2206 2207 2242 2243 3043 3111 3232 3410 3439 Size: 1 35 1 800 68 121 178 29 Cuts at: 3439 3730 4102 4256 4257 4483 5338 5389 5471 Size: 291 372 154 1 226 855 51 82 Cuts at: 5471 2206 Size: 5007 Fragments arranged by size: 5007 855 800 372 291 226 178 154 121 82 68 51 35 29 1 1 1 EcoRI G'AATT_C Cuts at: 5581 5959 5581 Size: 378 7894 EcoRV GAT'ATC Cuts at: 4060 4060 Size: 8272 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2680 5123 2680 Size: 2443 5829 FspI TGC'GCA Cuts at: 4478 6778 4478 Size: 2300 5972 HaeII r_GCGC'y Cuts at: 1678 4706 5241 5256 5296 7419 1678 Size: 3028 535 15 40 2123 2531 Fragments arranged by size: 3028 2531 2123 535 40 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3368 3400 4657 4689 4787 Size: 32 135 32 1796 32 1257 32 98 Cuts at: 4787 4819 6913 6945 6987 7019 1373 Size: 32 2094 32 42 32 2626 Fragments arranged by size: 2626 2094 1796 1257 135 98 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2269 5616 5862 669 Size: 988 281 331 3347 246 3079 Fragments arranged by size: 3347 3079 988 331 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8272 HpaI GTT'AAC Cuts at: 5862 5862 Size: 8272 KasI G'GCGC_C Cuts at: 5237 5252 5292 5237 Size: 15 40 8217 Fragments arranged by size: 8217 40 15 KpnI G_GTAC'C Cuts at: 2917 5597 2917 Size: 2680 5592 MfeI C'AATT_G Cuts at: 5871 5871 Size: 8272 MluI A'CGCG_T Cuts at: 8148 8148 Size: 8272 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7386 7570 1305 Size: 6081 184 2007 Fragments arranged by size: 6081 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2391 3423 10 Size: 54 1425 343 559 1032 4859 Fragments arranged by size: 4859 1425 1032 559 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2385 2532 2941 4031 5054 6230 6589 Size: 648 1219 147 409 1090 1023 1176 359 Cuts at: 6589 6748 518 Size: 159 2042 Fragments arranged by size: 2042 1219 1176 1090 1023 648 409 359 159 147 MspA1I CmG'CkG Cuts at: 1606 2125 2326 2672 2920 2974 3515 3748 3852 Size: 519 201 346 248 54 541 233 104 Cuts at: 3852 3938 4223 4301 5265 5688 6365 7513 7758 Size: 86 285 78 964 423 677 1148 245 Cuts at: 7758 1606 Size: 2120 Fragments arranged by size: 2120 1148 964 677 541 519 423 346 285 248 245 233 201 104 86 78 54 NaeI GCC'GGC Cuts at: 2780 3650 2780 Size: 870 7402 NarI GG'CG_CC Cuts at: 5238 5253 5293 5238 Size: 15 40 8217 Fragments arranged by size: 8217 40 15 NcoI C'CATG_G Cuts at: 513 1065 2004 2116 3817 4900 513 Size: 552 939 112 1701 1083 3885 Fragments arranged by size: 3885 1701 1083 939 552 112 NdeI CA'TA_TG Cuts at: 387 387 Size: 8272 NgoMIV G'CCGG_C Cuts at: 2778 3648 2778 Size: 870 7402 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8272 NotI GC'GGCC_GC Cuts at: 5719 5719 Size: 8272 NspI r_CATG'y Cuts at: 1132 1229 2221 5063 5630 7175 1132 Size: 97 992 2842 567 1545 2229 Fragments arranged by size: 2842 2229 1545 992 567 97 PasI CC'CwG_GG Cuts at: 1348 2203 2559 3108 3629 4444 4529 1348 Size: 855 356 549 521 815 85 5091 Fragments arranged by size: 5091 855 815 549 521 356 85 PciI A'CATG_T Cuts at: 7171 7171 Size: 8272 PflMI CCAn_nnn'nTGG Cuts at: 1108 2326 2900 4012 1108 Size: 1218 574 1112 5368 Fragments arranged by size: 5368 1218 1112 574 PfoI T'CCnGG_A Cuts at: 2457 3435 4392 4431 4590 2457 Size: 978 957 39 159 6139 Fragments arranged by size: 6139 978 957 159 39 PmeI GTTT'AAAC Cuts at: 5576 5576 Size: 8272 PmlI CAC'GTG Cuts at: 5170 5170 Size: 8272 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3083 3115 6341 6373 7854 7886 3083 Size: 32 3226 32 1481 32 3469 Fragments arranged by size: 3469 3226 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3410 3439 5471 3410 Size: 29 2032 6211 Fragments arranged by size: 6211 2032 29 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8272 PsiI TTA'TAA Cuts at: 5842 5842 Size: 8272 PspOMI G'GGCC_C Cuts at: 2206 2242 3231 3741 4101 4256 2206 Size: 36 989 510 360 155 6222 Fragments arranged by size: 6222 989 510 360 155 36 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2897 2929 2897 Size: 32 8240 PstI C_TGCA'G Cuts at: 830 2718 3322 3585 3806 3857 4551 5368 5624 Size: 1888 604 263 221 51 694 817 256 Cuts at: 5624 830 Size: 3478 Fragments arranged by size: 3478 1888 817 694 604 263 256 221 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8272 PvuII CAG'CTG Cuts at: 2672 2920 3515 5265 2672 Size: 248 595 1750 5679 Fragments arranged by size: 5679 1750 595 248 SacI G_AGCT'C Cuts at: 2470 2470 Size: 8272 SacII CC_GC'GG Cuts at: 3939 4302 3939 Size: 363 7909 SalI G'TCGA_C Cuts at: 1936 5614 1936 Size: 3678 4594 SanDI GG'GwC_CC Cuts at: 5471 5471 Size: 8272 SapI GCTCTTCn'nnn_ Cuts at: 2807 2807 Size: 8272 SbfI CC_TGCA'GG Cuts at: 5368 5624 5368 Size: 256 8016 ScaI AGT'ACT Cuts at: 4889 6520 8232 4889 Size: 1631 1712 4929 Fragments arranged by size: 4929 1712 1631 SexAI A'CCwGG_T Cuts at: 4038 5040 5379 4038 Size: 1002 339 6931 Fragments arranged by size: 6931 1002 339 SfcI C'TryA_G Cuts at: 826 1045 2714 3318 3581 3802 3853 4547 5364 Size: 219 1669 604 263 221 51 694 817 Cuts at: 5364 5620 6755 7436 7627 826 Size: 256 1135 681 191 1471 Fragments arranged by size: 1669 1471 1135 817 694 681 604 263 256 221 219 191 51 SfoI GGC'GCC Cuts at: 5239 5254 5294 5239 Size: 15 40 8217 Fragments arranged by size: 8217 40 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8272 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8272 SmaI CCC'GGG Cuts at: 1872 3444 4094 4108 4470 5599 1872 Size: 1572 650 14 362 1129 4545 Fragments arranged by size: 4545 1572 1129 650 362 14 SmlI C'TyrA_G Cuts at: 820 1017 1943 2100 2396 6376 7277 7539 820 Size: 197 926 157 296 3980 901 262 1553 Fragments arranged by size: 3980 1553 926 901 296 262 197 157 SnaBI TAC'GTA Cuts at: 493 6063 493 Size: 5570 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8272 SphI G_CATG'C Cuts at: 1132 5063 5630 1132 Size: 3931 567 3774 Fragments arranged by size: 3931 3774 567 SspI AAT'ATT Cuts at: 5 52 6196 5 Size: 47 6144 2081 Fragments arranged by size: 6144 2081 47 StuI AGG'CCT Cuts at: 1517 2859 3098 3663 5115 1517 Size: 1342 239 565 1452 4674 Fragments arranged by size: 4674 1452 1342 565 239 StyI C'CwwG_G Cuts at: 513 1065 2004 2116 2211 2664 2908 2939 3757 Size: 552 939 112 95 453 244 31 818 Cuts at: 3757 3817 4900 5711 513 Size: 60 1083 811 3074 Fragments arranged by size: 3074 1083 939 818 811 552 453 244 112 95 60 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2467 2984 3013 3711 3848 4196 6299 Size: 1255 887 517 29 698 137 348 2103 Cuts at: 6299 6316 6469 6654 7099 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2103 1498 1255 887 698 517 445 348 185 153 137 29 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4887 6518 8230 Size: 274 80 33 51 1443 2910 1631 1712 Cuts at: 8230 96 Size: 138 Fragments arranged by size: 2910 1712 1631 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3686 3718 4680 4712 5337 5369 5411 5443 8232 Size: 32 962 32 625 32 42 32 2789 Cuts at: 8232 8264 3686 Size: 32 3694 Fragments arranged by size: 3694 2789 962 625 42 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5042 1316 Size: 3726 4546 XbaI T'CTAG_A Cuts at: 5608 5608 Size: 8272 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4009 5079 4009 Size: 1070 7202 XhoI C'TCGA_G Cuts at: 1943 2100 1943 Size: 157 8115 XmaI C'CCGG_G Cuts at: 1870 3442 4092 4106 4468 5597 1870 Size: 1572 650 14 362 1129 4545 Fragments arranged by size: 4545 1572 1129 650 362 14 XmnI GAAnn'nnTTC Cuts at: 5102 6401 5102 Size: 1299 6973 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6080 276 Size: 53 83 186 936 4546 2468 Fragments arranged by size: 4546 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI FseI FspAI NruI NsiI PacI PspXI RsrII SfiI SrfI SwaI