Restriction map for
pFN21AB3279
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AgeI AhdI AocI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BcgI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsiWI BsmBI
BspDI BspHI BspLU11I BstAPI BstBI
Bsu36I CelII ClaI CpoI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoT14I EcoT22I FseI
FspI HindIII HpaI KpnI Ksp632I
MamI MfeI MunI NcoI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI Sse8387I SspI StuI StyI
SwaI Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 615 TCCGGA T^CCGGA
AclI 760 AACGTT AA^CGTT
AflII 891 CTTAAG C^TTAAG
AlwNI 148 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2061 GGGCCC GGGCC^C
Asp700I 414 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2515 TGGCCA TGG^CCA
BbsI 2295 GAAGAC GAAGAC(2/6)
BciVI 2524 GTATCC GTATCC(6/5)
BfrI 891 CTTAAG C^TTAAG
BpuAI 2295 GAAGAC GAAGAC(2/6)
BsaAI 1519 TACGTG [CT]AC^GT[GA]
BsaI 2761 GAGACC GGTCTC(1/5)
BseAI 615 TCCGGA T^CCGGA
BsgI 303 GTGCAG GTGCAG(16/14)
BsmAI 2761 GAGAC GTCTC(1/5)
BsmI 2477 GCATTC GAATGC(1/-1)
BspEI 615 TCCGGA T^CCGGA
BsrDI 64 GCAATG GCAATG(2/0)
BssSI 1573 CACGAG CACGAG(-5/-1)
Bst1107I 738 GTATAC GTA^TAC
BstXI 1988 CCACTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 738 GTATAC GTA^TAC
DraI 2605 TTTAAA TTT^AAA
EcoRI 258 GAATTC G^AATTC
EcoRV 481 GATATC GAT^ATC
KspI 210 CCGCGG CCGC^GG
MluNI 2515 TGGCCA TGG^CCA
MroI 615 TCCGGA T^CCGGA
MscI 2515 TGGCCA TGG^CCA
NdeI 919 CATATG CA^TATG
NspI 2444 GCATGC [GA]CATG^[CT]
Psp1406I 760 AACGTT AA^CGTT
PvuII 2595 CAGCTG CAG^CTG
SacI 1232 GAGCTC GAGCT^C
SacII 210 CCGCGG CCGC^GG
SmaI 2319 CCCGGG CCC^GGG
SphI 2444 GCATGC GCATG^C
XmaCI 2319 CCCGGG C^CCGGG
XmaI 2319 CCCGGG C^CCGGG
XmnI 414 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 328 GTAGAC GT^[AC][GT]AC
738 GTATAC
AcsI 258 GAATTC [GA]^AATT[CT]
2659 AAATTC
Alw44I 1528 GTGCAC G^TGCAC
2458 GTGCAC
Aor51HI 1502 AGCGCT AGC^GCT
1971 AGCGCT
ApaLI 1528 GTGCAC G^TGCAC
2458 GTGCAC
ApoI 258 GAATTC [GA]^AATT[CT]
2659 AAATTC
BbeI 1756 GGCGCC GGCGC^C
2022 GGCGCC
BclI 2099 TGATCA T^GATCA
2505 TGATCA
BsaWI 615 TCCGGA [AT]^CCGG[AT]
2635 TCCGGT
BsiEI 270 CGACCG CG[GA][CT]^CG
1747 CGGTCG
BsrFI 1686 GCCGGC [GA]^CCGG[CT]
1759 GCCGGC
BstEII 1152 GGTGACC G^GT[ACGT]ACC
1272 GGTTACC
BstPI 1152 GGTGACC G^GT[ACGT]ACC
1272 GGTTACC
Cfr10I 1686 GCCGGC [GA]^CCGG[CT]
1759 GCCGGC
Eco47III 1502 AGCGCT AGC^GCT
1971 AGCGCT
EcoNI 219 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2463 CCTAGAGGAGG
EcoO65I 1152 GGTGACC G^GT[ACGT]ACC
1272 GGTTACC
FbaI 2099 TGATCA T^GATCA
2505 TGATCA
KasI 1756 GGCGCC G^GCGCC
2022 GGCGCC
MluI 1811 ACGCGT A^CGCGT
1900 ACGCGT
MslI 126 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
787 CACCCCAATG
NaeI 1686 GCCGGC GCC^GGC
1759 GCCGGC
NarI 1756 GGCGCC GG^CGCC
2022 GGCGCC
NgoMIV 1686 GCCGGC G^CCGGC
1759 GCCGGC
PleI 445 GACTC GAGTC(4/5)
1798 GACTC
PpuMI 149 AGGACCT [GA]G^G[AT]CC[CT]
215 GGGACCT
SfoI 1756 GGCGCC GGC^GCC
2022 GGCGCC
SgrAI 1685 CGCCGGCG C[GA]^CCGG[CT]G
1758 CGCCGGCG
SmlI 9 CTCAAG C^T[CT][GA]AG
891 CTTAAG
XcmI 872 CCAAGTTCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1316 CCAGGGTGTCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrGI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII